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The map label for this gene is epsC [H]

Identifier: 75675265

GI number: 75675265

Start: 1173006

End: 1174949

Strand: Direct

Name: epsC [H]

Synonym: Nwi_1072

Alternate gene names: 75675265

Gene position: 1173006-1174949 (Clockwise)

Preceding gene: 75675264

Following gene: 75675267

Centisome position: 34.48

GC content: 60.55

Gene sequence:

>1944_bases
ATGAACCTCCGCCGAAAATTCACGCTCCGAAACGCCCTGATTGCGCTGCATGACCTGGTGGCGGTCGTGGCCGCTCTGTT
TGTGAGCTGCTATTTCCGTTTTGAGTATTCCGCCTTTACCGCTCGTCTGCCGTTGCTGATCGAGGTGCTGCCCTACTTTC
TGGCCGTCGCCGCGATTGTTTTGTATGTTTTCCATCTGACCACGGCAAAATGGCGATTTTTTTCGCTTCTGGATGCCCTC
AATATCGTCAAGGCCTCGACGCTTCTTGCGCTTGTCATTCTGGTCTTCGACTATGTCGTCATCGCGCCGGAGTTTTACGG
TGCGTTCTTCCTCGGAAAAAAGACGATCATCCTCTACTGGCTGTTGCAGATTCTTCTTCTGGCGGCCACGCGCTCCACCT
ATCGATACTTCCGCTACAGGCGGACTCTGGTTCACGTAAAGGCCGATGACGCGACGCCTGCGATCGTGGTCGGCCGCGCC
GCGGATGCCGAGATACTTTTCCGCTCGGTCGAGAGCGGAGCGGTGAAGCAGTTGCGTCCGGTCGGCCTGCTGTCGCCGTC
GCCGGCCGATATCGGCCAGACGATACGCGGCGTTTCAGTCTTGGGCGGAGTGGACGAACTCGAGGAGGTGATAGCTGATA
TCGCGGAGCGGCAGGAGCCGATCAAGCGCATCATCATGACGCCTTCAGCGTTCGAACCGGATGCCCGGCCCGAAGCGATT
CTCGCCCACGGCCGCAGACTTGGACTCAAGGTAAGCCGGTTGCCCTCGCTCGGGGAGGGAGGGGAGATTCCGCGCCTCAC
GCCGGTCGCCGTTGAAGACCTTCTGCTGCGCCCAAGCCGCACTATCGACTATGAGCGGCTCGAAGGATTGATCAAGAACA
AGTCGGTTGTCGTGACCGGCGGCGGCGGATCGATCGGATCCGAAATCTGCAAACGCGTCGTGACGTTCGGCGCGAGCCGC
CTGCTGATCGTCGAGAATTCCGAACCGGCGCTCTACGCGATCGTGGAGGAACTCGCCGCCTCCAGCCGGGCCGCCGTGGT
GGAAGGTCGTGTTGCCGACATTCGCGATCGCGAGCGGCTGATGCGTTTGATGAACGAATTCAAGCCTGATATCGTGTTTC
ATGCGGCTGCGCTCAAGCATGTTCCCATTCTCGAACGGGACTGGGACGAGGGCGTCAAGACCAACATTTTCGGTTCAGTC
AACGTCGCCGATGCAGCGCTGGCATCGGGCGCCGAGTCCATGGTGATGATCTCCACCGACAAGGCGATCGAACCGGTGTC
GATGCTCGGGCTGACCAAACGTTTCGCGGAGATGTATTGTCAGGCGCTGGATCGCGATCTGGCGGTGCGGGCGGCCGGCA
AGGTTCCGATGCGGCTGATTTCGGTTCGTTTCGGCAACGTGCTGGCGTCGAACGGGTCGGTGGTGCCGAAGTTCAAGGCG
CAGATCGAGGCGGGCGGTCCGGTGACCGTCACGCATCCGGACATGGTTCGCTACTTCATGACGATCCGCGAAGCCTGCGA
TCTCGTCATTACCGCGGCGAGCCATGCGGCGATGCCGGAAAGACATGACGTATCCGTGTATGTCCTGAACATGGGCCAGC
CGGTGAAGATCATCGATCTTGCCGAACGCATGATCAGCCTGTCCGGCTTGCAGCCTCACCGCGACATCGAGATTGTCTTT
ACCGGGGTTCGGCCCGGCGAACGAATGAACGAAATCTTGTTCGCCAGCGAGGAGCCGACGATCGAGATCGGCTTACCGGG
GATCCTGGCGGCAAAGCCGAACGAGCCGCCGATGGAGACGATCCGTGAGTGGATCGCGGTGCTCAAGGAAGCCGTCGCGA
GCGACAACAAGCTGGCGATCCGCTCGATCCTGAAAGACGCCGTGCCGGAGTTCGGCGGGCAGCCCGCGCAAACATCGCCT
CTTGCGCCCGATATGCCGCGATGA

Upstream 100 bases:

>100_bases
CATCGCTGGTGAAGCGCGCCGGAGGGGCATGATCGATCGTGTTCGACCGGCGCTTCCCCGTATCCCAATGACTTTCCGCC
TCTGACGATCTGGACGACGT

Downstream 100 bases:

>100_bases
ATCGCGGCGTCATTCGGTGCTCTCGACTGCGATCGATCCTTTCCGGCTCCTTTCGGCGCTGAAAATCCAGACGAGTCCCC
CGAGCGCGCCGACGACCAGA

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 647; Mature: 647

Protein sequence:

>647_residues
MNLRRKFTLRNALIALHDLVAVVAALFVSCYFRFEYSAFTARLPLLIEVLPYFLAVAAIVLYVFHLTTAKWRFFSLLDAL
NIVKASTLLALVILVFDYVVIAPEFYGAFFLGKKTIILYWLLQILLLAATRSTYRYFRYRRTLVHVKADDATPAIVVGRA
ADAEILFRSVESGAVKQLRPVGLLSPSPADIGQTIRGVSVLGGVDELEEVIADIAERQEPIKRIIMTPSAFEPDARPEAI
LAHGRRLGLKVSRLPSLGEGGEIPRLTPVAVEDLLLRPSRTIDYERLEGLIKNKSVVVTGGGGSIGSEICKRVVTFGASR
LLIVENSEPALYAIVEELAASSRAAVVEGRVADIRDRERLMRLMNEFKPDIVFHAAALKHVPILERDWDEGVKTNIFGSV
NVADAALASGAESMVMISTDKAIEPVSMLGLTKRFAEMYCQALDRDLAVRAAGKVPMRLISVRFGNVLASNGSVVPKFKA
QIEAGGPVTVTHPDMVRYFMTIREACDLVITAASHAAMPERHDVSVYVLNMGQPVKIIDLAERMISLSGLQPHRDIEIVF
TGVRPGERMNEILFASEEPTIEIGLPGILAAKPNEPPMETIREWIAVLKEAVASDNKLAIRSILKDAVPEFGGQPAQTSP
LAPDMPR

Sequences:

>Translated_647_residues
MNLRRKFTLRNALIALHDLVAVVAALFVSCYFRFEYSAFTARLPLLIEVLPYFLAVAAIVLYVFHLTTAKWRFFSLLDAL
NIVKASTLLALVILVFDYVVIAPEFYGAFFLGKKTIILYWLLQILLLAATRSTYRYFRYRRTLVHVKADDATPAIVVGRA
ADAEILFRSVESGAVKQLRPVGLLSPSPADIGQTIRGVSVLGGVDELEEVIADIAERQEPIKRIIMTPSAFEPDARPEAI
LAHGRRLGLKVSRLPSLGEGGEIPRLTPVAVEDLLLRPSRTIDYERLEGLIKNKSVVVTGGGGSIGSEICKRVVTFGASR
LLIVENSEPALYAIVEELAASSRAAVVEGRVADIRDRERLMRLMNEFKPDIVFHAAALKHVPILERDWDEGVKTNIFGSV
NVADAALASGAESMVMISTDKAIEPVSMLGLTKRFAEMYCQALDRDLAVRAAGKVPMRLISVRFGNVLASNGSVVPKFKA
QIEAGGPVTVTHPDMVRYFMTIREACDLVITAASHAAMPERHDVSVYVLNMGQPVKIIDLAERMISLSGLQPHRDIEIVF
TGVRPGERMNEILFASEEPTIEIGLPGILAAKPNEPPMETIREWIAVLKEAVASDNKLAIRSILKDAVPEFGGQPAQTSP
LAPDMPR
>Mature_647_residues
MNLRRKFTLRNALIALHDLVAVVAALFVSCYFRFEYSAFTARLPLLIEVLPYFLAVAAIVLYVFHLTTAKWRFFSLLDAL
NIVKASTLLALVILVFDYVVIAPEFYGAFFLGKKTIILYWLLQILLLAATRSTYRYFRYRRTLVHVKADDATPAIVVGRA
ADAEILFRSVESGAVKQLRPVGLLSPSPADIGQTIRGVSVLGGVDELEEVIADIAERQEPIKRIIMTPSAFEPDARPEAI
LAHGRRLGLKVSRLPSLGEGGEIPRLTPVAVEDLLLRPSRTIDYERLEGLIKNKSVVVTGGGGSIGSEICKRVVTFGASR
LLIVENSEPALYAIVEELAASSRAAVVEGRVADIRDRERLMRLMNEFKPDIVFHAAALKHVPILERDWDEGVKTNIFGSV
NVADAALASGAESMVMISTDKAIEPVSMLGLTKRFAEMYCQALDRDLAVRAAGKVPMRLISVRFGNVLASNGSVVPKFKA
QIEAGGPVTVTHPDMVRYFMTIREACDLVITAASHAAMPERHDVSVYVLNMGQPVKIIDLAERMISLSGLQPHRDIEIVF
TGVRPGERMNEILFASEEPTIEIGLPGILAAKPNEPPMETIREWIAVLKEAVASDNKLAIRSILKDAVPEFGGQPAQTSP
LAPDMPR

Specific function: Involved in biofilm formation [H]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 71051; Mature: 71051

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLRRKFTLRNALIALHDLVAVVAALFVSCYFRFEYSAFTARLPLLIEVLPYFLAVAAIV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHH
LYVFHLTTAKWRFFSLLDALNIVKASTLLALVILVFDYVVIAPEFYGAFFLGKKTIILYW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LLQILLLAATRSTYRYFRYRRTLVHVKADDATPAIVVGRAADAEILFRSVESGAVKQLRP
HHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEEECCCCHHHHHHHHHCCHHHHHCC
VGLLSPSPADIGQTIRGVSVLGGVDELEEVIADIAERQEPIKRIIMTPSAFEPDARPEAI
CCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
LAHGRRLGLKVSRLPSLGEGGEIPRLTPVAVEDLLLRPSRTIDYERLEGLIKNKSVVVTG
HHCCHHCCEEHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEC
GGGSIGSEICKRVVTFGASRLLIVENSEPALYAIVEELAASSRAAVVEGRVADIRDRERL
CCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEECCCHHHHHHHHHH
MRLMNEFKPDIVFHAAALKHVPILERDWDEGVKTNIFGSVNVADAALASGAESMVMISTD
HHHHHHCCCHHEEHHHHHHCCCCCCCCCCCCCCCCEECCCCHHHHHHHCCCCCEEEEECC
KAIEPVSMLGLTKRFAEMYCQALDRDLAVRAAGKVPMRLISVRFGNVLASNGSVVPKFKA
CCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHCCEEECCCCCCCCHHH
QIEAGGPVTVTHPDMVRYFMTIREACDLVITAASHAAMPERHDVSVYVLNMGQPVKIIDL
EECCCCCEEECCHHHHHHHHHHHHHHHEEEEECHHHCCCCCCCCEEEEEECCCCCHHHHH
AERMISLSGLQPHRDIEIVFTGVRPGERMNEILFASEEPTIEIGLPGILAAKPNEPPMET
HHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHEEECCCCEEEECCCEEEECCCCCCHHHH
IREWIAVLKEAVASDNKLAIRSILKDAVPEFGGQPAQTSPLAPDMPR
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNLRRKFTLRNALIALHDLVAVVAALFVSCYFRFEYSAFTARLPLLIEVLPYFLAVAAIV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHH
LYVFHLTTAKWRFFSLLDALNIVKASTLLALVILVFDYVVIAPEFYGAFFLGKKTIILYW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LLQILLLAATRSTYRYFRYRRTLVHVKADDATPAIVVGRAADAEILFRSVESGAVKQLRP
HHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEEECCCCHHHHHHHHHCCHHHHHCC
VGLLSPSPADIGQTIRGVSVLGGVDELEEVIADIAERQEPIKRIIMTPSAFEPDARPEAI
CCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
LAHGRRLGLKVSRLPSLGEGGEIPRLTPVAVEDLLLRPSRTIDYERLEGLIKNKSVVVTG
HHCCHHCCEEHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEC
GGGSIGSEICKRVVTFGASRLLIVENSEPALYAIVEELAASSRAAVVEGRVADIRDRERL
CCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEECCCHHHHHHHHHH
MRLMNEFKPDIVFHAAALKHVPILERDWDEGVKTNIFGSVNVADAALASGAESMVMISTD
HHHHHHCCCHHEEHHHHHHCCCCCCCCCCCCCCCCEECCCCHHHHHHHCCCCCEEEEECC
KAIEPVSMLGLTKRFAEMYCQALDRDLAVRAAGKVPMRLISVRFGNVLASNGSVVPKFKA
CCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHCCEEECCCCCCCCHHH
QIEAGGPVTVTHPDMVRYFMTIREACDLVITAASHAAMPERHDVSVYVLNMGQPVKIIDL
EECCCCCEEECCHHHHHHHHHHHHHHHEEEEECHHHCCCCCCCCEEEEEECCCCCHHHHH
AERMISLSGLQPHRDIEIVFTGVRPGERMNEILFASEEPTIEIGLPGILAAKPNEPPMET
HHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHEEECCCCEEEECCCEEEECCCCCCHHHH
IREWIAVLKEAVASDNKLAIRSILKDAVPEFGGQPAQTSPLAPDMPR
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]