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The map label for this gene is yjbJ [H]

Identifier: 75675045

GI number: 75675045

Start: 926329

End: 927339

Strand: Direct

Name: yjbJ [H]

Synonym: Nwi_0850

Alternate gene names: 75675045

Gene position: 926329-927339 (Clockwise)

Preceding gene: 75675044

Following gene: 75675048

Centisome position: 27.23

GC content: 66.86

Gene sequence:

>1011_bases
ATGACGCAGATCGGCACGTTCACGAAGACCAAGACCGGCTTCAGCGGTCGCCTCCGCACGCTCGCGCTCGACGTCGAGCG
CCCGTCGTTCCGATCCTTGTCTGCCGTCATCCCTCTGATCGATCGGATCGCGGATCATCCCTCCGACCCGATCGCCCGTC
AGGCGGCCGTCGCGCGCAGCGCAGGTCAAGGGCGGCCGCAGGGCCGGCGCAGCCTTGCCCTTGACCGAAGCGAGCACGGT
GGCAGGCTGATGGCGTCTCGGAATGGCGGGCCATGCACGGCCCTGTCATCGATCATCGCGACGTTGATCGCGTGCGGCGT
GATCGTTGTAGCCGCGCCTACCGCGTCGGCCGATCCGACACCCACAAATCGCGGCTCGACGGTTGCGCCGTCCGGTCCGT
GGGCGCCGCACATCGCCGAGGCGGCGAAGCGCTTCGCCATTCCCGAGCGCTGGATACGCGCGGTCATGGCCGTCGAGAGC
CACGGCGATCGGACCGCGCGATCGCCCAAGGGCGCGATCGGCTTGATGCAGATCATGCCGAAGACATGGGACGAGTTGCG
CGCGCGTCACGCACTCGGAAATGACCCTTGGCAACCGCGCGCCAACATCCTGGCCGGCGCGGCGTACCTGCGCGAGATGC
ATGACCGCTACGGCACGGTAGAAGCGATGCTTGCTGCTTACAATGCCGGCCCTGGCCGCTATGACAAGCATCTTGCCACC
GGCCAGGCGCTCCCCGCCGAAACCATCGACTATGTCGCGAAGATCACGCCGACGATCGACGTTACGGCCTCCGTTGTGCG
GTCCATTGGCCGTCCATTGCACCCGTCATGGTCACGCGCTCCGCTGTTCGTTGCGCGTTCCATCGCCGCAGGCGAGGACG
ATCGCGATGCCGGCGAGTCTCCTTCCGGCCGACCTTCGAACGGATTGCCGATTACCGGTCTATCAGCGCTCGTGCCGCCG
TCCGCCGGACTCTTCGTGCGCAGGCCTGAAGACGACGGAGGACAGCGATGA

Upstream 100 bases:

>100_bases
TGGACCACCGACGGCACCGATCCGGCCGCGGAATCCCTCCGCGCGCCGGTCTCCGACGAGCCGTGACGGCTCGCTTCTCC
AACCAGATGAGGATATCGCC

Downstream 100 bases:

>100_bases
ACATCGTGTTTCCTCGCTTCGCGAAGTGCGCAATCGTAGAGAGTGTGGAACGCTGCGGGGCAGCACAGGCAACGACCGAG
CGAATGCCTCCCGTAATCGG

Product: lytic transglycosylase, catalytic

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MTQIGTFTKTKTGFSGRLRTLALDVERPSFRSLSAVIPLIDRIADHPSDPIARQAAVARSAGQGRPQGRRSLALDRSEHG
GRLMASRNGGPCTALSSIIATLIACGVIVVAAPTASADPTPTNRGSTVAPSGPWAPHIAEAAKRFAIPERWIRAVMAVES
HGDRTARSPKGAIGLMQIMPKTWDELRARHALGNDPWQPRANILAGAAYLREMHDRYGTVEAMLAAYNAGPGRYDKHLAT
GQALPAETIDYVAKITPTIDVTASVVRSIGRPLHPSWSRAPLFVARSIAAGEDDRDAGESPSGRPSNGLPITGLSALVPP
SAGLFVRRPEDDGGQR

Sequences:

>Translated_336_residues
MTQIGTFTKTKTGFSGRLRTLALDVERPSFRSLSAVIPLIDRIADHPSDPIARQAAVARSAGQGRPQGRRSLALDRSEHG
GRLMASRNGGPCTALSSIIATLIACGVIVVAAPTASADPTPTNRGSTVAPSGPWAPHIAEAAKRFAIPERWIRAVMAVES
HGDRTARSPKGAIGLMQIMPKTWDELRARHALGNDPWQPRANILAGAAYLREMHDRYGTVEAMLAAYNAGPGRYDKHLAT
GQALPAETIDYVAKITPTIDVTASVVRSIGRPLHPSWSRAPLFVARSIAAGEDDRDAGESPSGRPSNGLPITGLSALVPP
SAGLFVRRPEDDGGQR
>Mature_335_residues
TQIGTFTKTKTGFSGRLRTLALDVERPSFRSLSAVIPLIDRIADHPSDPIARQAAVARSAGQGRPQGRRSLALDRSEHGG
RLMASRNGGPCTALSSIIATLIACGVIVVAAPTASADPTPTNRGSTVAPSGPWAPHIAEAAKRFAIPERWIRAVMAVESH
GDRTARSPKGAIGLMQIMPKTWDELRARHALGNDPWQPRANILAGAAYLREMHDRYGTVEAMLAAYNAGPGRYDKHLATG
QALPAETIDYVAKITPTIDVTASVVRSIGRPLHPSWSRAPLFVARSIAAGEDDRDAGESPSGRPSNGLPITGLSALVPPS
AGLFVRRPEDDGGQR

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 35641; Mature: 35510

Theoretical pI: Translated: 10.76; Mature: 10.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQIGTFTKTKTGFSGRLRTLALDVERPSFRSLSAVIPLIDRIADHPSDPIARQAAVARS
CCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
AGQGRPQGRRSLALDRSEHGGRLMASRNGGPCTALSSIIATLIACGVIVVAAPTASADPT
CCCCCCCCCHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCC
PTNRGSTVAPSGPWAPHIAEAAKRFAIPERWIRAVMAVESHGDRTARSPKGAIGLMQIMP
CCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
KTWDELRARHALGNDPWQPRANILAGAAYLREMHDRYGTVEAMLAAYNAGPGRYDKHLAT
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHC
GQALPAETIDYVAKITPTIDVTASVVRSIGRPLHPSWSRAPLFVARSIAAGEDDRDAGES
CCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCC
PSGRPSNGLPITGLSALVPPSAGLFVRRPEDDGGQR
CCCCCCCCCCCCCHHHHCCCCCCEEEECCCCCCCCC
>Mature Secondary Structure 
TQIGTFTKTKTGFSGRLRTLALDVERPSFRSLSAVIPLIDRIADHPSDPIARQAAVARS
CCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
AGQGRPQGRRSLALDRSEHGGRLMASRNGGPCTALSSIIATLIACGVIVVAAPTASADPT
CCCCCCCCCHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCC
PTNRGSTVAPSGPWAPHIAEAAKRFAIPERWIRAVMAVESHGDRTARSPKGAIGLMQIMP
CCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
KTWDELRARHALGNDPWQPRANILAGAAYLREMHDRYGTVEAMLAAYNAGPGRYDKHLAT
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHC
GQALPAETIDYVAKITPTIDVTASVVRSIGRPLHPSWSRAPLFVARSIAAGEDDRDAGES
CCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCC
PSGRPSNGLPITGLSALVPPSAGLFVRRPEDDGGQR
CCCCCCCCCCCCCHHHHCCCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]