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The map label for this gene is narK [H]

Identifier: 75674975

GI number: 75674975

Start: 868091

End: 869347

Strand: Direct

Name: narK [H]

Synonym: Nwi_0779

Alternate gene names: 75674975

Gene position: 868091-869347 (Clockwise)

Preceding gene: 75674974

Following gene: 75674976

Centisome position: 25.52

GC content: 52.59

Gene sequence:

>1257_bases
GTGGAACAATCAGCTGGCAAGGTCGAGACGTCGGGCGAACCGCAGGGCAACGCGCGCGTGCTGTGGATATCGACCATAGC
GTTTTCGCTAGGGTTCGCTATATGGGGAATGTTCTCGGCCCTCGCACCGTTTCTGATAGAGTGGTACCATTTTACAAGTA
CGCAGGTGCTTGTGTTGGCCGCTATGGAGCCGCTTTTTGCAGCTGCAATCAGCATTCCACTCGGTATTTGGACGGACAAG
TTCGGCGGACGAACGGTGTTTACGATTCTGCTGGTTTTGCTTTCAGTGGTTTTAATCTTGGGAACTTTTGCAGAGGGTTA
TTATTCCTTCTTGTTCCTTGGGTCGATGCTGGGGCTCGGTGGAGCAACCTTCGTGGTAGGTAACGCGCATGTTTCCTCGT
GGTATCCGAAGTCTAAGCAGGGTACTGCCTTGGGGTTGTTTGCCTTGGGTAATATAGGGATTACCTTTGGCATGATGGCT
GTCACCTTCGTCATTACAAGCGTTGCTGACCAGAGCGACCCTGAAGGTTGGCGTATTATATTTCCGATCTTTGCCATTCC
CACGTTGTTTATGGCGGCGATCTATATGTTCTTTACTTCTGATCCGCCAAACAGGAAGCTTAAGAATACGTCGACGCGAG
AAATTTGGGCTGTCTATCGGTCGGGTGTCGTTGTATGGCTGGTGCCAGGTCTCTATTGGGTGGCTTTTGGTACTTTGGTT
TTCTTTGCGTCTACGATGCCGACTTATTTGGTCGATCATTGGCACGTTGACGCTACCTCGGCGGTGGCGTTTTACACCCC
CGTGTTGGTGGTTTGCGTAGCAGTTACCCGACCGCTCGGTGGCTGGGCAGCTGACCGATACGACGTGCTGACGCTTCTAA
GTTGGATGTTTGGCGCTATGTCGGTTCTCGCGGTGCTGATGGCGTTGCAATTGTCTTTCAGCTTGGAGCTTGTAGCGATC
TACGGGTTGGCGTTGTTGTCAGGTGCCGCTGCGGCTGCGGTGATCAAGCTGATCCCGATCTATTTTGAGCACGTTGGTGC
GGTTAGCGGGTTGGCGAAGGCGGCTGGTGCTGCTTGTGGTTTCACGATGACCGCTGTGCTAGCTGCTAGCAATTTCCTGG
TGGGGGGATACACGCTCGGTTTTATCGTCTGGGCATTAATGAACATGGCAGCTTTTTATGTGTCGTTCTCTCGAGTGGGC
TTTAGAGATGTCAAGCCCATTGGCGCTCCAGTAGCTGAAGCCGTACCTGCTCATTGA

Upstream 100 bases:

>100_bases
TAGCGGCTGGTAGTCTTGCTAGGTCGACAATCTAGGTAGATAACCTGTTTTCTTAGAAGTAACGCAAGCTAAATAGCTAT
TTTCTTCGTAGGAGCTACCG

Downstream 100 bases:

>100_bases
TTGCCGTCAGGTCTCGGACATTTGATCGTCAGATTGAGCATTGTCACCGCCCCTAATGAGGGTGGTGACATATTGCGGTG
CAGAGCGTAGGGATTAGTAG

Product: major facilitator transporter

Products: NA

Alternate protein names: Nitrite facilitator [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MEQSAGKVETSGEPQGNARVLWISTIAFSLGFAIWGMFSALAPFLIEWYHFTSTQVLVLAAMEPLFAAAISIPLGIWTDK
FGGRTVFTILLVLLSVVLILGTFAEGYYSFLFLGSMLGLGGATFVVGNAHVSSWYPKSKQGTALGLFALGNIGITFGMMA
VTFVITSVADQSDPEGWRIIFPIFAIPTLFMAAIYMFFTSDPPNRKLKNTSTREIWAVYRSGVVVWLVPGLYWVAFGTLV
FFASTMPTYLVDHWHVDATSAVAFYTPVLVVCVAVTRPLGGWAADRYDVLTLLSWMFGAMSVLAVLMALQLSFSLELVAI
YGLALLSGAAAAAVIKLIPIYFEHVGAVSGLAKAAGAACGFTMTAVLAASNFLVGGYTLGFIVWALMNMAAFYVSFSRVG
FRDVKPIGAPVAEAVPAH

Sequences:

>Translated_418_residues
MEQSAGKVETSGEPQGNARVLWISTIAFSLGFAIWGMFSALAPFLIEWYHFTSTQVLVLAAMEPLFAAAISIPLGIWTDK
FGGRTVFTILLVLLSVVLILGTFAEGYYSFLFLGSMLGLGGATFVVGNAHVSSWYPKSKQGTALGLFALGNIGITFGMMA
VTFVITSVADQSDPEGWRIIFPIFAIPTLFMAAIYMFFTSDPPNRKLKNTSTREIWAVYRSGVVVWLVPGLYWVAFGTLV
FFASTMPTYLVDHWHVDATSAVAFYTPVLVVCVAVTRPLGGWAADRYDVLTLLSWMFGAMSVLAVLMALQLSFSLELVAI
YGLALLSGAAAAAVIKLIPIYFEHVGAVSGLAKAAGAACGFTMTAVLAASNFLVGGYTLGFIVWALMNMAAFYVSFSRVG
FRDVKPIGAPVAEAVPAH
>Mature_418_residues
MEQSAGKVETSGEPQGNARVLWISTIAFSLGFAIWGMFSALAPFLIEWYHFTSTQVLVLAAMEPLFAAAISIPLGIWTDK
FGGRTVFTILLVLLSVVLILGTFAEGYYSFLFLGSMLGLGGATFVVGNAHVSSWYPKSKQGTALGLFALGNIGITFGMMA
VTFVITSVADQSDPEGWRIIFPIFAIPTLFMAAIYMFFTSDPPNRKLKNTSTREIWAVYRSGVVVWLVPGLYWVAFGTLV
FFASTMPTYLVDHWHVDATSAVAFYTPVLVVCVAVTRPLGGWAADRYDVLTLLSWMFGAMSVLAVLMALQLSFSLELVAI
YGLALLSGAAAAAVIKLIPIYFEHVGAVSGLAKAAGAACGFTMTAVLAASNFLVGGYTLGFIVWALMNMAAFYVSFSRVG
FRDVKPIGAPVAEAVPAH

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44864; Mature: 44864

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQSAGKVETSGEPQGNARVLWISTIAFSLGFAIWGMFSALAPFLIEWYHFTSTQVLVLA
CCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
AMEPLFAAAISIPLGIWTDKFGGRTVFTILLVLLSVVLILGTFAEGYYSFLFLGSMLGLG
HHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GATFVVGNAHVSSWYPKSKQGTALGLFALGNIGITFGMMAVTFVITSVADQSDPEGWRII
CCEEEEECCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
FPIFAIPTLFMAAIYMFFTSDPPNRKLKNTSTREIWAVYRSGVVVWLVPGLYWVAFGTLV
EHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCEEEEHHHHHHHHHHHHHH
FFASTMPTYLVDHWHVDATSAVAFYTPVLVVCVAVTRPLGGWAADRYDVLTLLSWMFGAM
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
SVLAVLMALQLSFSLELVAIYGLALLSGAAAAAVIKLIPIYFEHVGAVSGLAKAAGAACG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FTMTAVLAASNFLVGGYTLGFIVWALMNMAAFYVSFSRVGFRDVKPIGAPVAEAVPAH
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCC
>Mature Secondary Structure
MEQSAGKVETSGEPQGNARVLWISTIAFSLGFAIWGMFSALAPFLIEWYHFTSTQVLVLA
CCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
AMEPLFAAAISIPLGIWTDKFGGRTVFTILLVLLSVVLILGTFAEGYYSFLFLGSMLGLG
HHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GATFVVGNAHVSSWYPKSKQGTALGLFALGNIGITFGMMAVTFVITSVADQSDPEGWRII
CCEEEEECCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
FPIFAIPTLFMAAIYMFFTSDPPNRKLKNTSTREIWAVYRSGVVVWLVPGLYWVAFGTLV
EHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCEEEEHHHHHHHHHHHHHH
FFASTMPTYLVDHWHVDATSAVAFYTPVLVVCVAVTRPLGGWAADRYDVLTLLSWMFGAM
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
SVLAVLMALQLSFSLELVAIYGLALLSGAAAAAVIKLIPIYFEHVGAVSGLAKAAGAACG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FTMTAVLAASNFLVGGYTLGFIVWALMNMAAFYVSFSRVGFRDVKPIGAPVAEAVPAH
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9384377; 9353933 [H]