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The map label for this gene is narX [H]

Identifier: 75674974

GI number: 75674974

Start: 867182

End: 867859

Strand: Direct

Name: narX [H]

Synonym: Nwi_0778

Alternate gene names: 75674974

Gene position: 867182-867859 (Clockwise)

Preceding gene: 75674973

Following gene: 75674975

Centisome position: 25.49

GC content: 50.74

Gene sequence:

>678_bases
ATGTATGACGTATTCCTGTTCGCGGTTCTGCCATACTTGGCGATCGTGGTGGCGGTTCTGGGTGGTGTTCATCGGTATCG
GACGAATCGCTTCTCGTATTCGACGTTGTCTTCGCAGCTCTTGGAGAATCGCCGTTTGTTCTGGGGATCTGTACCTTGGC
ATTACGGTATTGTGGTTGTCTTGTTAGCCCATATGATCGGGTTCCTTGTTCCCGGTATGTGGTCCGCCCTGACATCGACT
CCCACGGCACTTTACACGGTTGAGTTGATAGGTTTTGCATTTGGTTTTGCTGCCTTGATAGGCCTATGCGTGTTGATAGT
GCGTCGATTTACGACTGAGCGAATTCTTGCTGTAACCAGCCCGATGGATGGCATACTGCTAGTTGCGTTGCTCGTTCAGG
TTGCGCTTGGTTTTTGGGTTTCGCTGGCTTACAGGTGGGGTGCTGACTGGTATATCGATACGGCTGTGCCTTGGTTGATT
TCTTTGGTGTCGCTAAACCCACAGACCCATTATGTGTCAGCTTTGCCCTTTCCAGTTAAGGCGCACATGCTCACGGGGTT
CCTTGTAATTGCACTGTTCCCATTTTCTCGGTTGGTGCATTTGATCATGTTCCCGTTCTGGTACCTGTGGCGCCCCTATC
AGATCCAGATCAGAAATACTGTGAGGTCCGCGCATTAG

Upstream 100 bases:

>100_bases
CATTTCAAGGTGTATAAGCCACTTGCGGAGGTCGCAGCAGACGACCTGCTGGATTGGCGAGCGTGAGAGGAATGTCCCAT
GTGTGACGGAGGCAAATTCT

Downstream 100 bases:

>100_bases
CTACTTGGCGAAGTAGTGAGGAATTGTTAAGGTGTTATTCTCGCCGGCGAGTTGCACTCGCCGGCGAGAATTATAAGTAG
TTAACGCGCGGGTCGAGAAT

Product: nitrate reductase gamma subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MYDVFLFAVLPYLAIVVAVLGGVHRYRTNRFSYSTLSSQLLENRRLFWGSVPWHYGIVVVLLAHMIGFLVPGMWSALTST
PTALYTVELIGFAFGFAALIGLCVLIVRRFTTERILAVTSPMDGILLVALLVQVALGFWVSLAYRWGADWYIDTAVPWLI
SLVSLNPQTHYVSALPFPVKAHMLTGFLVIALFPFSRLVHLIMFPFWYLWRPYQIQIRNTVRSAH

Sequences:

>Translated_225_residues
MYDVFLFAVLPYLAIVVAVLGGVHRYRTNRFSYSTLSSQLLENRRLFWGSVPWHYGIVVVLLAHMIGFLVPGMWSALTST
PTALYTVELIGFAFGFAALIGLCVLIVRRFTTERILAVTSPMDGILLVALLVQVALGFWVSLAYRWGADWYIDTAVPWLI
SLVSLNPQTHYVSALPFPVKAHMLTGFLVIALFPFSRLVHLIMFPFWYLWRPYQIQIRNTVRSAH
>Mature_225_residues
MYDVFLFAVLPYLAIVVAVLGGVHRYRTNRFSYSTLSSQLLENRRLFWGSVPWHYGIVVVLLAHMIGFLVPGMWSALTST
PTALYTVELIGFAFGFAALIGLCVLIVRRFTTERILAVTSPMDGILLVALLVQVALGFWVSLAYRWGADWYIDTAVPWLI
SLVSLNPQTHYVSALPFPVKAHMLTGFLVIALFPFSRLVHLIMFPFWYLWRPYQIQIRNTVRSAH

Specific function: Does not seem to have nitrate reductase activity [H]

COG id: COG2181

COG function: function code C; Nitrate reductase gamma subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the nitrate reductase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787480, Length=228, Percent_Identity=29.8245614035088, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1787738, Length=218, Percent_Identity=30.2752293577982, Blast_Score=91, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020945
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR003816
- InterPro:   IPR003765 [H]

Pfam domain/function: PF00384 Molybdopterin; PF02613 Nitrate_red_del; PF02665 Nitrate_red_gam [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 25598; Mature: 25598

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDVFLFAVLPYLAIVVAVLGGVHRYRTNRFSYSTLSSQLLENRRLFWGSVPWHYGIVVV
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEECCCCHHHHHHHH
LLAHMIGFLVPGMWSALTSTPTALYTVELIGFAFGFAALIGLCVLIVRRFTTERILAVTS
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PMDGILLVALLVQVALGFWVSLAYRWGADWYIDTAVPWLISLVSLNPQTHYVSALPFPVK
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCCCEEECCCCCHH
AHMLTGFLVIALFPFSRLVHLIMFPFWYLWRPYQIQIRNTVRSAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEHHHHHHCCC
>Mature Secondary Structure
MYDVFLFAVLPYLAIVVAVLGGVHRYRTNRFSYSTLSSQLLENRRLFWGSVPWHYGIVVV
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEECCCCHHHHHHHH
LLAHMIGFLVPGMWSALTSTPTALYTVELIGFAFGFAALIGLCVLIVRRFTTERILAVTS
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PMDGILLVALLVQVALGFWVSLAYRWGADWYIDTAVPWLISLVSLNPQTHYVSALPFPVK
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCCCEEECCCCCHH
AHMLTGFLVIALFPFSRLVHLIMFPFWYLWRPYQIQIRNTVRSAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]