Click here to switch to the map view.

The map label for this gene is rpfC [H]

Identifier: 75674945

GI number: 75674945

Start: 835353

End: 836609

Strand: Direct

Name: rpfC [H]

Synonym: Nwi_0749

Alternate gene names: 75674945

Gene position: 835353-836609 (Clockwise)

Preceding gene: 75674944

Following gene: 75674947

Centisome position: 24.55

GC content: 66.43

Gene sequence:

>1257_bases
ATGGCGCCGACATCGCGCGGGTTGCGCAAGCCGCGGAAGCCTACGAGGCGACCGGCGAAGAAGCATGTTCCGAAGACGCC
GGCGAAGCCTGCAAAGGCGGGGCGGCCGCGTTCGCAGTCAACGTCCGGCGTCGTCGAGGACGCGCTCGCCGCGTTTGCGC
ATGAAGTGCGGACGCCGCTCACCGGAATTCTCGCCGTCAGTGATCTGCTCGCCACCTCCGATCTCGGCGAGCGCGAGCGG
CGCTGGGTCGAGACCATCAAGGCCGGCGCCGATCATCTCGCCAGCCTGGCGACGCTGTTCGTCGATGCGGCGCGAAGCCG
CCGTTCGGCGCCGGGCGGACGGCAGGATTTCTTCGATCTGCGAGCGCTGGCGCGCAACGCCGGCGATTCACTGGCCGGCC
GCGCAACCGCCAAGGGCCTGCGCTCCACTGTTGAAATTTCAGACGGGTTGCCCGCGTTCGTGACCGGCGATGCCGTCCGC
CTGCGTGCGGCGCTGGAGAATCTCATCGACAATGCGGTCAAGTTTACCGAGCAGGGTGACGTCATCCTCAAGGTTACGGC
GTCGCGACCGTCAGAAGGAAAAACAGGCGTGACCTTCGTGATCTCCGATAGCGGGATCGGCCTGTCGCTCACCGAGATCA
GGCGGCTGTTCCGGCCGTATTCGCAGGCCAATGTCTCGATCGCATCGCGCTTCGGCGGCGCCGGTCTCGGGCTCTCCTCG
GTGAAGCAACTGGCGCGCGCGATGGGGGGAGATGTCACGGCGACGCAGCGGCGCGGCGGCGGCACCACGTTCACGTTCAC
CGTTCTGCTGACGCCAGCCGAGGGACCGGTGGCGATTCAGTCCGGCGACGATCTCCACAGGCCGTCGGATGTTTCCGGTG
CGCTCCGGATTCTCAGTGTTGAAGATAATCCGTTTGGGCGCGTCGTGCTCAACACGATCCTTACCGAACTCGGCCATCAG
GCGGAGTTCGTCGGACGGGGCGAGGATGCGGTTCAGCGCATCGCGCAAGGAGGGTTCGATGCGGTGCTGATGGATATGGT
GCTGCCTGGAATCACCGGCATCGAGGCGATCGGGCGTATCCGGGCGCTTGATGCGCCGGGGGGGCGCGTTGCGATTATCG
GCGTGTCCGGCAGGAGCGACGATGAGGCGGCATCGCGGGCCGCCGGCGGCGATGCGTTTCTGGTCAAGCCTGTTTCTCCC
CGGGCTCTAGCGACTGCGCTGCTTGAAGCGAAACACCGCGCGGCAACCGCGACTTGA

Upstream 100 bases:

>100_bases
TTGGTTAGCCGTAAAGCATCTTCGCATGACAAACCCATCCACGACGTGACTCGCGCGTTCGAGCCGTGGCATGCTGGCGT
GGGGTGGGGAAAGCAAGATC

Downstream 100 bases:

>100_bases
TGATCGCCGCGTTCAGTTCGCCGCCGAAAACGAAGATCGCGGCGATGAAATAGAGGAACACCAGCGCGATGATGACCGAT
GCGAGGCCGGCATACATCGT

Product: Signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MAPTSRGLRKPRKPTRRPAKKHVPKTPAKPAKAGRPRSQSTSGVVEDALAAFAHEVRTPLTGILAVSDLLATSDLGERER
RWVETIKAGADHLASLATLFVDAARSRRSAPGGRQDFFDLRALARNAGDSLAGRATAKGLRSTVEISDGLPAFVTGDAVR
LRAALENLIDNAVKFTEQGDVILKVTASRPSEGKTGVTFVISDSGIGLSLTEIRRLFRPYSQANVSIASRFGGAGLGLSS
VKQLARAMGGDVTATQRRGGGTTFTFTVLLTPAEGPVAIQSGDDLHRPSDVSGALRILSVEDNPFGRVVLNTILTELGHQ
AEFVGRGEDAVQRIAQGGFDAVLMDMVLPGITGIEAIGRIRALDAPGGRVAIIGVSGRSDDEAASRAAGGDAFLVKPVSP
RALATALLEAKHRAATAT

Sequences:

>Translated_418_residues
MAPTSRGLRKPRKPTRRPAKKHVPKTPAKPAKAGRPRSQSTSGVVEDALAAFAHEVRTPLTGILAVSDLLATSDLGERER
RWVETIKAGADHLASLATLFVDAARSRRSAPGGRQDFFDLRALARNAGDSLAGRATAKGLRSTVEISDGLPAFVTGDAVR
LRAALENLIDNAVKFTEQGDVILKVTASRPSEGKTGVTFVISDSGIGLSLTEIRRLFRPYSQANVSIASRFGGAGLGLSS
VKQLARAMGGDVTATQRRGGGTTFTFTVLLTPAEGPVAIQSGDDLHRPSDVSGALRILSVEDNPFGRVVLNTILTELGHQ
AEFVGRGEDAVQRIAQGGFDAVLMDMVLPGITGIEAIGRIRALDAPGGRVAIIGVSGRSDDEAASRAAGGDAFLVKPVSP
RALATALLEAKHRAATAT
>Mature_417_residues
APTSRGLRKPRKPTRRPAKKHVPKTPAKPAKAGRPRSQSTSGVVEDALAAFAHEVRTPLTGILAVSDLLATSDLGERERR
WVETIKAGADHLASLATLFVDAARSRRSAPGGRQDFFDLRALARNAGDSLAGRATAKGLRSTVEISDGLPAFVTGDAVRL
RAALENLIDNAVKFTEQGDVILKVTASRPSEGKTGVTFVISDSGIGLSLTEIRRLFRPYSQANVSIASRFGGAGLGLSSV
KQLARAMGGDVTATQRRGGGTTFTFTVLLTPAEGPVAIQSGDDLHRPSDVSGALRILSVEDNPFGRVVLNTILTELGHQA
EFVGRGEDAVQRIAQGGFDAVLMDMVLPGITGIEAIGRIRALDAPGGRVAIIGVSGRSDDEAASRAAGGDAFLVKPVSPR
ALATALLEAKHRAATAT

Specific function: Involved in the positive regulation of synthesis of extracellular enzymes and polysaccharide, and hence in pathogenicity. Can restore function to the non-pathogenic mutant 8237 [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI48994928, Length=371, Percent_Identity=33.4231805929919, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI87081816, Length=345, Percent_Identity=31.8840579710145, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI1789149, Length=237, Percent_Identity=32.0675105485232, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI1788713, Length=376, Percent_Identity=27.1276595744681, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI145693157, Length=246, Percent_Identity=29.6747967479675, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1790436, Length=220, Percent_Identity=26.8181818181818, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786600, Length=249, Percent_Identity=24.4979919678715, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 43711; Mature: 43580

Theoretical pI: Translated: 11.06; Mature: 11.06

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPTSRGLRKPRKPTRRPAKKHVPKTPAKPAKAGRPRSQSTSGVVEDALAAFAHEVRTPL
CCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHH
TGILAVSDLLATSDLGERERRWVETIKAGADHLASLATLFVDAARSRRSAPGGRQDFFDL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
RALARNAGDSLAGRATAKGLRSTVEISDGLPAFVTGDAVRLRAALENLIDNAVKFTEQGD
HHHHHCCCCCHHHHHHHHHHHHEEECCCCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCC
VILKVTASRPSEGKTGVTFVISDSGIGLSLTEIRRLFRPYSQANVSIASRFGGAGLGLSS
EEEEEECCCCCCCCCCEEEEEECCCCCEEHHHHHHHHCCHHHCCHHHHHHCCCCCCCHHH
VKQLARAMGGDVTATQRRGGGTTFTFTVLLTPAEGPVAIQSGDDLHRPSDVSGALRILSV
HHHHHHHHCCCCEEECCCCCCCEEEEEEEEECCCCCEEEECCCCCCCCCCCCCEEEEEEE
EDNPFGRVVLNTILTELGHQAEFVGRGEDAVQRIAQGGFDAVLMDMVLPGITGIEAIGRI
CCCCCHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHE
RALDAPGGRVAIIGVSGRSDDEAASRAAGGDAFLVKPVSPRALATALLEAKHRAATAT
EEECCCCCEEEEEEECCCCCHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
APTSRGLRKPRKPTRRPAKKHVPKTPAKPAKAGRPRSQSTSGVVEDALAAFAHEVRTPL
CCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHH
TGILAVSDLLATSDLGERERRWVETIKAGADHLASLATLFVDAARSRRSAPGGRQDFFDL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
RALARNAGDSLAGRATAKGLRSTVEISDGLPAFVTGDAVRLRAALENLIDNAVKFTEQGD
HHHHHCCCCCHHHHHHHHHHHHEEECCCCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCC
VILKVTASRPSEGKTGVTFVISDSGIGLSLTEIRRLFRPYSQANVSIASRFGGAGLGLSS
EEEEEECCCCCCCCCCEEEEEECCCCCEEHHHHHHHHCCHHHCCHHHHHHCCCCCCCHHH
VKQLARAMGGDVTATQRRGGGTTFTFTVLLTPAEGPVAIQSGDDLHRPSDVSGALRILSV
HHHHHHHHCCCCEEECCCCCCCEEEEEEEEECCCCCEEEECCCCCCCCCCCCCEEEEEEE
EDNPFGRVVLNTILTELGHQAEFVGRGEDAVQRIAQGGFDAVLMDMVLPGITGIEAIGRI
CCCCCHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHE
RALDAPGGRVAIIGVSGRSDDEAASRAAGGDAFLVKPVSPRALATALLEAKHRAATAT
EEECCCCCEEEEEEECCCCCHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1645442 [H]