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The map label for this gene is gltX1 [H]

Identifier: 75674944

GI number: 75674944

Start: 834359

End: 835231

Strand: Direct

Name: gltX1 [H]

Synonym: Nwi_0748

Alternate gene names: 75674944

Gene position: 834359-835231 (Clockwise)

Preceding gene: 75674940

Following gene: 75674945

Centisome position: 24.52

GC content: 65.75

Gene sequence:

>873_bases
ATGCCGCCACCCGTTTTCCGTTTCGCGCCGTCGCCGAACGGCTTTCTGCATCTCGGCCACGCTTTTTCGGCGCTCATGAA
TTTTGAATCCGCCCGGCGGGGGCAAGGGCGTTTCCTGCTGCGGATCGAGGACATTGATGCAGCCCGGTGCCGTCCCGAGT
TCGAAGCGGCGATCTATCAAGATCTCGCCTGGCTTGGCCTGTCGTGGGAGACGCCGGTGCGGCGGCAGTCGGAGCACCTT
GCCGACTATCGCGCGGCGCTCGACCGGCTGGCCGCGTTGGGGTTGCTCTATCCGAGTTTCGAGAGCCGCGCCGAGATCGC
GGCCCTGGTGAACGCGCGTGAGATGGATGGAGGCTGGCCACGCGACCCCGACGGCGCTCCGCTCTATCCGGGAACGGCGA
AATCGCTCGCGGCGACCGAACGCGAACGGCGGCTGCGGTCCGGCGCGCCCTTCGCCCTGCGTCTGGATATGGAGGCGGCG
CGTCGGCTCGCGGGCGATCTTACCTGGATAGAGCGGGGCTCCGGGCCGGACGGGGAGACGGGCGAAATTCTCGCGCGCCC
GGAAGCCTGGGGTGACGTCATTCTCGCCCGCAAGGATATCCCGACCAGTTACCATTTGTCGGTCGTGATCGACGATGCGC
TCCAGGGGGTGACCGAAGTGACGCGCGGGCAGGATCTGTTCAGGTCGACCGCCGTGCACCGGCTGCTGCAAGAAATCCTG
GACCTGCCTCAGCCGGTTTACCGTCACCATGATCTGATCCGCGATGCCGAGGGGAAGAAGTTGTCCAAGTCGTCAAGAGC
GATTGGGTTGCGGGAGTTGCGGAGCGGCGGAATGACAACCGCGGAGCTTCGCAGCCTTGTCGGCCTACCTTGA

Upstream 100 bases:

>100_bases
CAGCGCGCCGACCGCGTCGTCGAGATCGGATTGGGTTTCCAGGCGAACCGTCATTCGTTAGGACTTCAGCCGTCACGGCC
GATTATGCAAGCAAGGATCA

Downstream 100 bases:

>100_bases
AACCGCCGCAACGCGGCTGCGTTGGTTAGCCGTAAAGCATCTTCGCATGACAAACCCATCCACGACGTGACTCGCGCGTT
CGAGCCGTGGCATGCTGGCG

Product: glutamyl-Q tRNA(Asp) synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1 [H]

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MPPPVFRFAPSPNGFLHLGHAFSALMNFESARRGQGRFLLRIEDIDAARCRPEFEAAIYQDLAWLGLSWETPVRRQSEHL
ADYRAALDRLAALGLLYPSFESRAEIAALVNAREMDGGWPRDPDGAPLYPGTAKSLAATERERRLRSGAPFALRLDMEAA
RRLAGDLTWIERGSGPDGETGEILARPEAWGDVILARKDIPTSYHLSVVIDDALQGVTEVTRGQDLFRSTAVHRLLQEIL
DLPQPVYRHHDLIRDAEGKKLSKSSRAIGLRELRSGGMTTAELRSLVGLP

Sequences:

>Translated_290_residues
MPPPVFRFAPSPNGFLHLGHAFSALMNFESARRGQGRFLLRIEDIDAARCRPEFEAAIYQDLAWLGLSWETPVRRQSEHL
ADYRAALDRLAALGLLYPSFESRAEIAALVNAREMDGGWPRDPDGAPLYPGTAKSLAATERERRLRSGAPFALRLDMEAA
RRLAGDLTWIERGSGPDGETGEILARPEAWGDVILARKDIPTSYHLSVVIDDALQGVTEVTRGQDLFRSTAVHRLLQEIL
DLPQPVYRHHDLIRDAEGKKLSKSSRAIGLRELRSGGMTTAELRSLVGLP
>Mature_289_residues
PPPVFRFAPSPNGFLHLGHAFSALMNFESARRGQGRFLLRIEDIDAARCRPEFEAAIYQDLAWLGLSWETPVRRQSEHLA
DYRAALDRLAALGLLYPSFESRAEIAALVNAREMDGGWPRDPDGAPLYPGTAKSLAATERERRLRSGAPFALRLDMEAAR
RLAGDLTWIERGSGPDGETGEILARPEAWGDVILARKDIPTSYHLSVVIDDALQGVTEVTRGQDLFRSTAVHRLLQEILD
LPQPVYRHHDLIRDAEGKKLSKSSRAIGLRELRSGGMTTAELRSLVGLP

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=289, Percent_Identity=30.1038062283737, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1788743, Length=284, Percent_Identity=31.3380281690141, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI226510925, Length=266, Percent_Identity=34.5864661654135, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1786895, Length=91, Percent_Identity=37.3626373626374, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71993394, Length=268, Percent_Identity=28.3582089552239, Blast_Score=94, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6324540, Length=284, Percent_Identity=30.9859154929577, Blast_Score=121, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24665321, Length=279, Percent_Identity=31.5412186379928, Blast_Score=115, Evalue=4e-26,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 32182; Mature: 32051

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPPVFRFAPSPNGFLHLGHAFSALMNFESARRGQGRFLLRIEDIDAARCRPEFEAAIYQ
CCCCCCEECCCCCCEEEHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHCCHHHHHHHHH
DLAWLGLSWETPVRRQSEHLADYRAALDRLAALGLLYPSFESRAEIAALVNAREMDGGWP
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
RDPDGAPLYPGTAKSLAATERERRLRSGAPFALRLDMEAARRLAGDLTWIERGSGPDGET
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHCCCEEECCCCCCCCC
GEILARPEAWGDVILARKDIPTSYHLSVVIDDALQGVTEVTRGQDLFRSTAVHRLLQEIL
CCEEECCCCCCCEEEEECCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLPQPVYRHHDLIRDAEGKKLSKSSRAIGLRELRSGGMTTAELRSLVGLP
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
PPPVFRFAPSPNGFLHLGHAFSALMNFESARRGQGRFLLRIEDIDAARCRPEFEAAIYQ
CCCCCEECCCCCCEEEHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHCCHHHHHHHHH
DLAWLGLSWETPVRRQSEHLADYRAALDRLAALGLLYPSFESRAEIAALVNAREMDGGWP
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
RDPDGAPLYPGTAKSLAATERERRLRSGAPFALRLDMEAARRLAGDLTWIERGSGPDGET
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHCCCEEECCCCCCCCC
GEILARPEAWGDVILARKDIPTSYHLSVVIDDALQGVTEVTRGQDLFRSTAVHRLLQEIL
CCEEECCCCCCCEEEEECCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLPQPVYRHHDLIRDAEGKKLSKSSRAIGLRELRSGGMTTAELRSLVGLP
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA