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The map label for this gene is rutF [H]

Identifier: 75674883

GI number: 75674883

Start: 764480

End: 765061

Strand: Direct

Name: rutF [H]

Synonym: Nwi_0686

Alternate gene names: 75674883

Gene position: 764480-765061 (Clockwise)

Preceding gene: 75674882

Following gene: 75674884

Centisome position: 22.47

GC content: 58.76

Gene sequence:

>582_bases
ATGAATCTAGCTGTTCGAGACATTTCGCGGGGACGCGAGATCGGGTCAGGCGAGTTCCGCAACGCCATGCGCCATCTGGC
CGGCGGCGTCAGCGTCATTACGGCCGGACGCGGCGACGATATTTCGGGTATGACGGTTACGTCAGTCGCCTCGCTGGCAA
TCGAGCCTGCAACCTTGACCGTCGCGATCAACCGAAAAGCGTCAACATTGCCGTTGCTCCTGCGATATGGCGCCTTTGGC
GTCAATATTCTGACTGGCGGCCAGGCCGGTATTGCTGAACGATTCACCGGCAAGGATGGGCATAAGGGAGCGGCACGCTT
TGCCGGCAGTTCCTGGACAACGGGTGCGTTTGGCGTGCCGCTGCTCAAGGATGCGCTGGTTGCCATCACCTGCGAGCTGG
AAGAAACCATCGAGCGGCATTCTCACGTTATCGCGCTTGGCCGTGTTGTTCATCTGGCGGCCTCGGAAGGAGACTCATCA
CTCGCCTATTGGCGAGGCGATTACCTTGCAATTGAACGAATCAGTGGCATATCGCGAACCGCTGGTGGTCCTTTACCGAC
CGTTCATGCCTCGGATGAGTGA

Upstream 100 bases:

>100_bases
CGCCGCGATCGAGGGATCGATCCTGCAGGAACTGTTGAAAGAGGATCGGTCGACCGATGCTTGATGAACGGGACGGATCA
TTCTTTAATTTGAGGCCATC

Downstream 100 bases:

>100_bases
GGATCGAGAGCCAATCTCACGCGCCGGATAGCCAGATAAAAGAAGCTCCTTTGTAGAGTTCCCTGCCGGCAGCGAAGTGA
TGGAGATTTGGTTGTATATC

Product: flavin reductase-like, FMN-binding

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MNLAVRDISRGREIGSGEFRNAMRHLAGGVSVITAGRGDDISGMTVTSVASLAIEPATLTVAINRKASTLPLLLRYGAFG
VNILTGGQAGIAERFTGKDGHKGAARFAGSSWTTGAFGVPLLKDALVAITCELEETIERHSHVIALGRVVHLAASEGDSS
LAYWRGDYLAIERISGISRTAGGPLPTVHASDE

Sequences:

>Translated_193_residues
MNLAVRDISRGREIGSGEFRNAMRHLAGGVSVITAGRGDDISGMTVTSVASLAIEPATLTVAINRKASTLPLLLRYGAFG
VNILTGGQAGIAERFTGKDGHKGAARFAGSSWTTGAFGVPLLKDALVAITCELEETIERHSHVIALGRVVHLAASEGDSS
LAYWRGDYLAIERISGISRTAGGPLPTVHASDE
>Mature_193_residues
MNLAVRDISRGREIGSGEFRNAMRHLAGGVSVITAGRGDDISGMTVTSVASLAIEPATLTVAINRKASTLPLLLRYGAFG
VNILTGGQAGIAERFTGKDGHKGAARFAGSSWTTGAFGVPLLKDALVAITCELEETIERHSHVIALGRVVHLAASEGDSS
LAYWRGDYLAIERISGISRTAGGPLPTVHASDE

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=160, Percent_Identity=29.375, Blast_Score=77, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019917
- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 20124; Mature: 20124

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLAVRDISRGREIGSGEFRNAMRHLAGGVSVITAGRGDDISGMTVTSVASLAIEPATLT
CCCCHHHHHCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHEECCEEEE
VAINRKASTLPLLLRYGAFGVNILTGGQAGIAERFTGKDGHKGAARFAGSSWTTGAFGVP
EEECCCCCHHHHHHHHCCCEEEEEECCCCCCHHHCCCCCCCCCHHHCCCCCCCCCCCCCH
LLKDALVAITCELEETIERHSHVIALGRVVHLAASEGDSSLAYWRGDYLAIERISGISRT
HHHHCCEEEEECHHHHHHHHHHEEEEHHEEEEEECCCCCCEEEECCCEEEEEHHCCCCCC
AGGPLPTVHASDE
CCCCCCEECCCCC
>Mature Secondary Structure
MNLAVRDISRGREIGSGEFRNAMRHLAGGVSVITAGRGDDISGMTVTSVASLAIEPATLT
CCCCHHHHHCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHEECCEEEE
VAINRKASTLPLLLRYGAFGVNILTGGQAGIAERFTGKDGHKGAARFAGSSWTTGAFGVP
EEECCCCCHHHHHHHHCCCEEEEEECCCCCCHHHCCCCCCCCCHHHCCCCCCCCCCCCCH
LLKDALVAITCELEETIERHSHVIALGRVVHLAASEGDSSLAYWRGDYLAIERISGISRT
HHHHCCEEEEECHHHHHHHHHHEEEEHHEEEEEECCCCCCEEEECCCEEEEEHHCCCCCC
AGGPLPTVHASDE
CCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA