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The map label for this gene is cysI [H]

Identifier: 75674789

GI number: 75674789

Start: 668804

End: 670534

Strand: Direct

Name: cysI [H]

Synonym: Nwi_0591

Alternate gene names: 75674789

Gene position: 668804-670534 (Clockwise)

Preceding gene: 75674788

Following gene: 75674790

Centisome position: 19.66

GC content: 62.68

Gene sequence:

>1731_bases
GTGGACGCAAAGACACAACCCGATCGCAGCCGCGATGTCTCCCAGCCGCTCGACAAGCTCGGACCGGATGAAACGCTGAA
GGCCAACAGCGACTATCTGCGTGGCACGATCAAGCAGAGTCTGGCCGACGAGATCACGTCGGCGGTGACGGCGAACGACG
CCAAGCTGATGAAGTTCTTCGGAATTTATCAGCAGGATGACCGGGATATCCGCGACGAGCGACGGCGTCAGAAGCTGGAG
GCGGCGTTCTCGTTCATGGTGCGGGTGCGGCTGCCGGGCGGCGTGTGCAGTCCCGGCCAGTGGCTGAAACTCGACGAGCT
GGCGCGCAACTACGCGGGCGACACGCTGCGGCTGACCACGCGCCAGACGTTCCAGTTCCATCGCGTTATGAAGCACAACC
TGCGCAGCCTGATCCAGGGGCTGCGCGATATCCTGCTCGACACCAGGGCGGCCTGCGGCGACGACACCCGTGGCGTCATG
AGCACCGTCAATCCGGACCTGTCGAAGCTGCACGCCGAGGTTTATGCGCTCGCCAAGCGAGCGAGCGACCATGCGGTGCA
CAGGACCGGCGCCTACAAGGAAATCTGGTACGAAGCCGAGCGCGAGCAAACCGATGGGCCGGAGGAGCCTTTCTACGGAC
GCACCTATATGCCGCGGAAGTTCAAGATCGGCTTCGCGATTCCGCCCAGCAACGATATCGACGTCTACGGCCAGGATCTC
GGCTTCATCGCCATCGCGCATCGCGGCAAGCTGAAGGGCTTCAACGTCGCCATCGGCGGCGGCCTTGGCCGGACCGATCA
GTCGCCGAAGACCTATCCGCGACTGGCCAGCGTGATCGGCTATATCGACGCGGACAAGCTGTTTCCGACAATCGACGCGG
TGATGTCGGTCCAGCGCGATTACGGCGACCGTCTCGACCGCTTGCACGCTCGCTTCAAGTACACCATCGACGAAAAGGGC
ATCGACTGGATCAAGGCGGAAACCGAACGCCGTCTCGGTTTCGCACTCAAGCCCGAGCAGCCCTACACCTTCACCTCGAA
CGGTGATCCGCTCGGCTGGGTCAAGGGCGAGGATGGTCGCGAGCATTGCGCCCTGTTCATCCAGAACGGCCGCGTGATCA
ATACGCCGGACCATCCGATGATGGATGGCTTGCGGGCCATCGCGCAGGTCCACAAGGGTATGTTCCGCGTCACGCCGAAC
CAGAATCTCATCATTGCCGACATCGCCTCCAGGGATCGGCCCGAAATCGAGGCGCTGATGAAGGAATACGGGCTCGATCA
ATTTGAGACCCGCAGCGGCTTGCGCCTCAACTCGATGGCCTGCGTCGCGCTGCCGACATGCGGTCTGGCGATGGCCGAAA
GCGAGCGCTATCTCCCGGATCTCCTCACCAAGATCGAGGCGATTCTCAACACTTACGGACTGACGGACGACCCGATCACC
ATCCGGATGACCGGATGCCCCAACGGCTGCGCGCGTCCCTATATCGCCGAGATCGGCCTCACCGGCCGCGCGCCCGGCAA
GTACAATCTCTATCTCGGCGGCGGCTTCCACGGCGAGCGGCTCAACAAGATGTATCTGGAGAATGTCGGGGAGCCCGCGA
TTCTGGAGGCGCTCGACAAGACGCTCGGCCATTATGCCCGCGACCGCAAGCCCGGCGAGCACTTCGGCGATTTCGCCATT
CGCGCCGGCTATGTCGTCGAGGTCAAGGAAGGGCGGTTCTTCAACGATTGA

Upstream 100 bases:

>100_bases
GCCGAACATGGCGGCCTCGATCGCGAGGAGGCGGGCGCCTATCTCTCGGCGCTGCAGCGTGATCGCCGTTATCGGCTCGA
CGTTTATTGAGGCCAGTTTG

Downstream 100 bases:

>100_bases
GCTGGGGCCTGTGACTGGTTTCCGCGAACCGGCTTTTGTTCTCGAAGCGAGAAGATAGCGCTGTCCTATAACGACGCACA
CTCTTTGTGATCCATGCGCC

Product: sulfite reductase subunit beta

Products: NA

Alternate protein names: SiR-HP; SiRHP [H]

Number of amino acids: Translated: 576; Mature: 576

Protein sequence:

>576_residues
MDAKTQPDRSRDVSQPLDKLGPDETLKANSDYLRGTIKQSLADEITSAVTANDAKLMKFFGIYQQDDRDIRDERRRQKLE
AAFSFMVRVRLPGGVCSPGQWLKLDELARNYAGDTLRLTTRQTFQFHRVMKHNLRSLIQGLRDILLDTRAACGDDTRGVM
STVNPDLSKLHAEVYALAKRASDHAVHRTGAYKEIWYEAEREQTDGPEEPFYGRTYMPRKFKIGFAIPPSNDIDVYGQDL
GFIAIAHRGKLKGFNVAIGGGLGRTDQSPKTYPRLASVIGYIDADKLFPTIDAVMSVQRDYGDRLDRLHARFKYTIDEKG
IDWIKAETERRLGFALKPEQPYTFTSNGDPLGWVKGEDGREHCALFIQNGRVINTPDHPMMDGLRAIAQVHKGMFRVTPN
QNLIIADIASRDRPEIEALMKEYGLDQFETRSGLRLNSMACVALPTCGLAMAESERYLPDLLTKIEAILNTYGLTDDPIT
IRMTGCPNGCARPYIAEIGLTGRAPGKYNLYLGGGFHGERLNKMYLENVGEPAILEALDKTLGHYARDRKPGEHFGDFAI
RAGYVVEVKEGRFFND

Sequences:

>Translated_576_residues
MDAKTQPDRSRDVSQPLDKLGPDETLKANSDYLRGTIKQSLADEITSAVTANDAKLMKFFGIYQQDDRDIRDERRRQKLE
AAFSFMVRVRLPGGVCSPGQWLKLDELARNYAGDTLRLTTRQTFQFHRVMKHNLRSLIQGLRDILLDTRAACGDDTRGVM
STVNPDLSKLHAEVYALAKRASDHAVHRTGAYKEIWYEAEREQTDGPEEPFYGRTYMPRKFKIGFAIPPSNDIDVYGQDL
GFIAIAHRGKLKGFNVAIGGGLGRTDQSPKTYPRLASVIGYIDADKLFPTIDAVMSVQRDYGDRLDRLHARFKYTIDEKG
IDWIKAETERRLGFALKPEQPYTFTSNGDPLGWVKGEDGREHCALFIQNGRVINTPDHPMMDGLRAIAQVHKGMFRVTPN
QNLIIADIASRDRPEIEALMKEYGLDQFETRSGLRLNSMACVALPTCGLAMAESERYLPDLLTKIEAILNTYGLTDDPIT
IRMTGCPNGCARPYIAEIGLTGRAPGKYNLYLGGGFHGERLNKMYLENVGEPAILEALDKTLGHYARDRKPGEHFGDFAI
RAGYVVEVKEGRFFND
>Mature_576_residues
MDAKTQPDRSRDVSQPLDKLGPDETLKANSDYLRGTIKQSLADEITSAVTANDAKLMKFFGIYQQDDRDIRDERRRQKLE
AAFSFMVRVRLPGGVCSPGQWLKLDELARNYAGDTLRLTTRQTFQFHRVMKHNLRSLIQGLRDILLDTRAACGDDTRGVM
STVNPDLSKLHAEVYALAKRASDHAVHRTGAYKEIWYEAEREQTDGPEEPFYGRTYMPRKFKIGFAIPPSNDIDVYGQDL
GFIAIAHRGKLKGFNVAIGGGLGRTDQSPKTYPRLASVIGYIDADKLFPTIDAVMSVQRDYGDRLDRLHARFKYTIDEKG
IDWIKAETERRLGFALKPEQPYTFTSNGDPLGWVKGEDGREHCALFIQNGRVINTPDHPMMDGLRAIAQVHKGMFRVTPN
QNLIIADIASRDRPEIEALMKEYGLDQFETRSGLRLNSMACVALPTCGLAMAESERYLPDLLTKIEAILNTYGLTDDPIT
IRMTGCPNGCARPYIAEIGLTGRAPGKYNLYLGGGFHGERLNKMYLENVGEPAILEALDKTLGHYARDRKPGEHFGDFAI
RAGYVVEVKEGRFFND

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate [H]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=548, Percent_Identity=48.1751824817518, Blast_Score=538, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6322597, Length=587, Percent_Identity=45.144804088586, Blast_Score=548, Evalue=1e-156,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011786
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 64770; Mature: 64770

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAKTQPDRSRDVSQPLDKLGPDETLKANSDYLRGTIKQSLADEITSAVTANDAKLMKFF
CCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
GIYQQDDRDIRDERRRQKLEAAFSFMVRVRLPGGVCSPGQWLKLDELARNYAGDTLRLTT
CCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCEEHHHHHHHHCCCEEEEEH
RQTFQFHRVMKHNLRSLIQGLRDILLDTRAACGDDTRGVMSTVNPDLSKLHAEVYALAKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHH
ASDHAVHRTGAYKEIWYEAEREQTDGPEEPFYGRTYMPRKFKIGFAIPPSNDIDVYGQDL
HCCHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEECCCC
GFIAIAHRGKLKGFNVAIGGGLGRTDQSPKTYPRLASVIGYIDADKLFPTIDAVMSVQRD
CEEEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
YGDRLDRLHARFKYTIDEKGIDWIKAETERRLGFALKPEQPYTFTSNGDPLGWVKGEDGR
HHHHHHHHHHHHEEEECCCCCCHHHHHHHHHCCEEECCCCCEEECCCCCEEEEECCCCCC
EHCALFIQNGRVINTPDHPMMDGLRAIAQVHKGMFRVTPNQNLIIADIASRDRPEIEALM
CEEEEEEECCEEECCCCCHHHHHHHHHHHHHCCEEEECCCCCEEEEECCCCCCHHHHHHH
KEYGLDQFETRSGLRLNSMACVALPTCGLAMAESERYLPDLLTKIEAILNTYGLTDDPIT
HHHCCCHHHHCCCCEECCEEEEEECCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEE
IRMTGCPNGCARPYIAEIGLTGRAPGKYNLYLGGGFHGERLNKMYLENVGEPAILEALDK
EEECCCCCCCCCCEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHH
TLGHYARDRKPGEHFGDFAIRAGYVVEVKEGRFFND
HHHHHHHCCCCCHHHCCCEEECCEEEEEECCCCCCC
>Mature Secondary Structure
MDAKTQPDRSRDVSQPLDKLGPDETLKANSDYLRGTIKQSLADEITSAVTANDAKLMKFF
CCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
GIYQQDDRDIRDERRRQKLEAAFSFMVRVRLPGGVCSPGQWLKLDELARNYAGDTLRLTT
CCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCEEHHHHHHHHCCCEEEEEH
RQTFQFHRVMKHNLRSLIQGLRDILLDTRAACGDDTRGVMSTVNPDLSKLHAEVYALAKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHH
ASDHAVHRTGAYKEIWYEAEREQTDGPEEPFYGRTYMPRKFKIGFAIPPSNDIDVYGQDL
HCCHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEECCCC
GFIAIAHRGKLKGFNVAIGGGLGRTDQSPKTYPRLASVIGYIDADKLFPTIDAVMSVQRD
CEEEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
YGDRLDRLHARFKYTIDEKGIDWIKAETERRLGFALKPEQPYTFTSNGDPLGWVKGEDGR
HHHHHHHHHHHHEEEECCCCCCHHHHHHHHHCCEEECCCCCEEECCCCCEEEEECCCCCC
EHCALFIQNGRVINTPDHPMMDGLRAIAQVHKGMFRVTPNQNLIIADIASRDRPEIEALM
CEEEEEEECCEEECCCCCHHHHHHHHHHHHHCCEEEECCCCCEEEEECCCCCCHHHHHHH
KEYGLDQFETRSGLRLNSMACVALPTCGLAMAESERYLPDLLTKIEAILNTYGLTDDPIT
HHHCCCHHHHCCCCEECCEEEEEECCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEE
IRMTGCPNGCARPYIAEIGLTGRAPGKYNLYLGGGFHGERLNKMYLENVGEPAILEALDK
EEECCCCCCCCCCEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHH
TLGHYARDRKPGEHFGDFAIRAGYVVEVKEGRFFND
HHHHHHHCCCCCHHHCCCEEECCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA