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The map label for this gene is cysJ [H]

Identifier: 75674788

GI number: 75674788

Start: 666952

End: 668793

Strand: Direct

Name: cysJ [H]

Synonym: Nwi_0590

Alternate gene names: 75674788

Gene position: 666952-668793 (Clockwise)

Preceding gene: 75674787

Following gene: 75674789

Centisome position: 19.6

GC content: 64.12

Gene sequence:

>1842_bases
ATGACAAAATTGGAATTTCCAGGTTCAGCACTCACGACGGATCAGTGGACGCAGGTCAACCAGCTCGCGACGTCTCTCAG
TCATGAACAGGCGATCTGGATCAGCGGCTATTTCGCGGGTCTCAGCCATCGGGCCCGGGCTGGCGCTGCGGCCACGGAAA
TCGTGGAGCTTGCACCGCCTTCGCAGGTCGGCGCCGACGGCGCTGCTGCGACGCGGCTCCTGACGGTCCTGTTCGCAAGC
GAGACCGGAAACAGCAAGACCCTCGCTAAAGCGCTGGTCGAAAAGGCCGCGGCCCAGGGAATCCAGGCGCGTCTCGCCGA
TATGGCTGAGTACAAGACGCGCGGCCTCAAGGACGAGCAGGATCTGCTCGTCATCACCAGCACTCACGGCGAGGGCGATG
CCCCGCAGACCGCCGTCGGTTTCTTTGAATTTCTGGAGAGCCGCAAGGCTCCCAAGCTGCCGCAACTGCGTTACGCGGTG
CTGGCGCTGGGCGATTCGACCTACGAGAAATACTGCGAGGCGGGCAAGCGGGTCGACCGGCGGCTCGAAGAACTGGGGGC
GCAACGGCTCGCCGATCGTGTCGACTGCGATGTCGATTACGAGGATGCCGCCGATGCGTGGATCGCATCGGTCGTCACCA
AGCTTGCGCCGGCCGCGCAGGCGCCTGCCTCCGCTCCGGGTGCATCGGCGGCGACGATGCGGAGCGGTGCGCCGTCGCCT
GCGACATTCGATAAAAAGAATCCGTTTCAGGCCGCGGTCATCGACAATATCGTGCTGACGGGACGCGGATCGACCAAGGA
AACGCGCCATATCGAGTTGTCGCTGGCGGATTCGGGTTTGACCTATCAGCCCGGCGATGCGCTCGGCGTGGTGCCGCGTA
ACGATCCGGCGCTGGTGGAAGCCATACTTGAGAAACTGTCGCTGTCCGCCGACGCTCCTGTCACGGTGAAGCAGGGTACG
ATAAGCCTTGGTGAGGCGCTGAGCGGCACATTCGAGATCACGGCGCTCACGCCGCGCTTTCTGGATCTTTGGTCCGAACT
CACGGGCTCCGATGAACTGCAGGCGTTGCGCGCGCCCGATCAGAAGGAGGCGCGCGCGGCCTTCCAGCACAACTATCACA
TTCTGGATGTCATCGGCCGTTTTCCGGCCCCCGGCATCGATGCCGCGCGGCTCGTCGCGGGTCTCAGGCCGTTGCAGCCG
CGGCTTTATTCGATCGCTTCGAGCCTTGCCGCCGCCCCGGACGAGGCGCATCTTACGGTCAGCACGGTGCGCTATACGCT
GCACGACCTGCCGCGCACCGGCGTCGCATCCGGCTATCTTGCCGCGCGTACCGAGGCGGACGCCACGGTTCCGGTCTACA
TCCAGTCCAACGATCACTTCCATCTGCCGGATGACGATGTGCCGATCCTGATGATCGGCGCGGGCACCGGCGTCGCGCCG
TATCGTGCTTTCATGCAGGAGCGTGAAGCCAGCGGCGCATCCGGCCGGTCATGGCTGTTTTTCGGCGAACGCAATTTCCG
CAGCGACTTCCTCTATCAGGTCGAGTGGCAGGAACTGCTCAAGAATGGCGTGCTGAGCCGGCTCGATCTGGCGTTCTCGC
GGGATGCCGCGCCCAAGACCTATGTGCAGGATCGCCTGCGCCGCCAGGGCCGTGATGTTTACGCATGGCTCGAGGAAGGC
GCGTATGTCTACGTCTGCGGCGACAGCGCCCACATGGCGCCTGACGTTCACGCGGCGTTGACCGAGATCGTCGCCGAACA
TGGCGGCCTCGATCGCGAGGAGGCGGGCGCCTATCTCTCGGCGCTGCAGCGTGATCGCCGTTATCGGCTCGACGTTTATT
GA

Upstream 100 bases:

>100_bases
AAACGGATGCAGAGGCTTTCCATTTCGTGCGGCGTCTCGACCAGACCTGTGAAGTGTCAATCGCGGCGGCCGGCAGTTCG
CTCCCGAGGTTTTGCTTGCG

Downstream 100 bases:

>100_bases
GGCCAGTTTGGTGGACGCAAAGACACAACCCGATCGCAGCCGCGATGTCTCCCAGCCGCTCGACAAGCTCGGACCGGATG
AAACGCTGAAGGCCAACAGC

Product: sulphite reductase (NADPH) flavoprotein, alpha chain

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 613; Mature: 612

Protein sequence:

>613_residues
MTKLEFPGSALTTDQWTQVNQLATSLSHEQAIWISGYFAGLSHRARAGAAATEIVELAPPSQVGADGAAATRLLTVLFAS
ETGNSKTLAKALVEKAAAQGIQARLADMAEYKTRGLKDEQDLLVITSTHGEGDAPQTAVGFFEFLESRKAPKLPQLRYAV
LALGDSTYEKYCEAGKRVDRRLEELGAQRLADRVDCDVDYEDAADAWIASVVTKLAPAAQAPASAPGASAATMRSGAPSP
ATFDKKNPFQAAVIDNIVLTGRGSTKETRHIELSLADSGLTYQPGDALGVVPRNDPALVEAILEKLSLSADAPVTVKQGT
ISLGEALSGTFEITALTPRFLDLWSELTGSDELQALRAPDQKEARAAFQHNYHILDVIGRFPAPGIDAARLVAGLRPLQP
RLYSIASSLAAAPDEAHLTVSTVRYTLHDLPRTGVASGYLAARTEADATVPVYIQSNDHFHLPDDDVPILMIGAGTGVAP
YRAFMQEREASGASGRSWLFFGERNFRSDFLYQVEWQELLKNGVLSRLDLAFSRDAAPKTYVQDRLRRQGRDVYAWLEEG
AYVYVCGDSAHMAPDVHAALTEIVAEHGGLDREEAGAYLSALQRDRRYRLDVY

Sequences:

>Translated_613_residues
MTKLEFPGSALTTDQWTQVNQLATSLSHEQAIWISGYFAGLSHRARAGAAATEIVELAPPSQVGADGAAATRLLTVLFAS
ETGNSKTLAKALVEKAAAQGIQARLADMAEYKTRGLKDEQDLLVITSTHGEGDAPQTAVGFFEFLESRKAPKLPQLRYAV
LALGDSTYEKYCEAGKRVDRRLEELGAQRLADRVDCDVDYEDAADAWIASVVTKLAPAAQAPASAPGASAATMRSGAPSP
ATFDKKNPFQAAVIDNIVLTGRGSTKETRHIELSLADSGLTYQPGDALGVVPRNDPALVEAILEKLSLSADAPVTVKQGT
ISLGEALSGTFEITALTPRFLDLWSELTGSDELQALRAPDQKEARAAFQHNYHILDVIGRFPAPGIDAARLVAGLRPLQP
RLYSIASSLAAAPDEAHLTVSTVRYTLHDLPRTGVASGYLAARTEADATVPVYIQSNDHFHLPDDDVPILMIGAGTGVAP
YRAFMQEREASGASGRSWLFFGERNFRSDFLYQVEWQELLKNGVLSRLDLAFSRDAAPKTYVQDRLRRQGRDVYAWLEEG
AYVYVCGDSAHMAPDVHAALTEIVAEHGGLDREEAGAYLSALQRDRRYRLDVY
>Mature_612_residues
TKLEFPGSALTTDQWTQVNQLATSLSHEQAIWISGYFAGLSHRARAGAAATEIVELAPPSQVGADGAAATRLLTVLFASE
TGNSKTLAKALVEKAAAQGIQARLADMAEYKTRGLKDEQDLLVITSTHGEGDAPQTAVGFFEFLESRKAPKLPQLRYAVL
ALGDSTYEKYCEAGKRVDRRLEELGAQRLADRVDCDVDYEDAADAWIASVVTKLAPAAQAPASAPGASAATMRSGAPSPA
TFDKKNPFQAAVIDNIVLTGRGSTKETRHIELSLADSGLTYQPGDALGVVPRNDPALVEAILEKLSLSADAPVTVKQGTI
SLGEALSGTFEITALTPRFLDLWSELTGSDELQALRAPDQKEARAAFQHNYHILDVIGRFPAPGIDAARLVAGLRPLQPR
LYSIASSLAAAPDEAHLTVSTVRYTLHDLPRTGVASGYLAARTEADATVPVYIQSNDHFHLPDDDVPILMIGAGTGVAPY
RAFMQEREASGASGRSWLFFGERNFRSDFLYQVEWQELLKNGVLSRLDLAFSRDAAPKTYVQDRLRRQGRDVYAWLEEGA
YVYVCGDSAHMAPDVHAALTEIVAEHGGLDREEAGAYLSALQRDRRYRLDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI7657393, Length=593, Percent_Identity=31.1973018549747, Blast_Score=236, Evalue=4e-62,
Organism=Homo sapiens, GI127139033, Length=606, Percent_Identity=30.3630363036304, Blast_Score=235, Evalue=8e-62,
Organism=Homo sapiens, GI221316705, Length=602, Percent_Identity=30.8970099667774, Blast_Score=232, Evalue=7e-61,
Organism=Homo sapiens, GI24041029, Length=599, Percent_Identity=32.0534223706177, Blast_Score=231, Evalue=2e-60,
Organism=Homo sapiens, GI221316709, Length=593, Percent_Identity=31.0286677908938, Blast_Score=228, Evalue=2e-59,
Organism=Homo sapiens, GI10835173, Length=658, Percent_Identity=30.6990881458967, Blast_Score=213, Evalue=5e-55,
Organism=Homo sapiens, GI221316707, Length=519, Percent_Identity=30.2504816955684, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI40254422, Length=643, Percent_Identity=29.8600311041991, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI169790956, Length=503, Percent_Identity=28.8270377733598, Blast_Score=187, Evalue=3e-47,
Organism=Homo sapiens, GI169790958, Length=503, Percent_Identity=28.8270377733598, Blast_Score=186, Evalue=4e-47,
Organism=Escherichia coli, GI1789123, Length=607, Percent_Identity=44.9752883031301, Blast_Score=503, Evalue=1e-143,
Organism=Escherichia coli, GI1789154, Length=147, Percent_Identity=30.6122448979592, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17554134, Length=598, Percent_Identity=30.1003344481605, Blast_Score=220, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17566446, Length=587, Percent_Identity=27.93867120954, Blast_Score=155, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI17531441, Length=146, Percent_Identity=34.9315068493151, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI32563863, Length=125, Percent_Identity=36, Blast_Score=79, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321832, Length=641, Percent_Identity=28.0811232449298, Blast_Score=174, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6325305, Length=629, Percent_Identity=22.4165341812401, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6321143, Length=391, Percent_Identity=28.6445012787724, Blast_Score=130, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6322597, Length=124, Percent_Identity=34.6774193548387, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI17137192, Length=609, Percent_Identity=31.1986863711002, Blast_Score=228, Evalue=7e-60,
Organism=Drosophila melanogaster, GI24582192, Length=518, Percent_Identity=31.6602316602317, Blast_Score=199, Evalue=6e-51,
Organism=Drosophila melanogaster, GI78706872, Length=663, Percent_Identity=29.5625942684766, Blast_Score=196, Evalue=4e-50,
Organism=Drosophila melanogaster, GI24583543, Length=663, Percent_Identity=29.7134238310709, Blast_Score=195, Evalue=7e-50,
Organism=Drosophila melanogaster, GI78706876, Length=663, Percent_Identity=29.7134238310709, Blast_Score=195, Evalue=7e-50,
Organism=Drosophila melanogaster, GI24660907, Length=600, Percent_Identity=26, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24660903, Length=600, Percent_Identity=26, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24647438, Length=418, Percent_Identity=27.7511961722488, Blast_Score=102, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 66301; Mature: 66170

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLEFPGSALTTDQWTQVNQLATSLSHEQAIWISGYFAGLSHRARAGAAATEIVELAPP
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCHHHHHHHHCCCC
SQVGADGAAATRLLTVLFASETGNSKTLAKALVEKAAAQGIQARLADMAEYKTRGLKDEQ
CCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DLLVITSTHGEGDAPQTAVGFFEFLESRKAPKLPQLRYAVLALGDSTYEKYCEAGKRVDR
CEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCHHEEEEEEECCHHHHHHHHHHHHHHH
RLEELGAQRLADRVDCDVDYEDAADAWIASVVTKLAPAAQAPASAPGASAATMRSGAPSP
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHCCCCCCCCCCHHHHCCCCCCC
ATFDKKNPFQAAVIDNIVLTGRGSTKETRHIELSLADSGLTYQPGDALGVVPRNDPALVE
CCCCCCCCCHHHHHCCEEEECCCCCCCCEEEEEEEECCCCEECCCCEEEECCCCCHHHHH
AILEKLSLSADAPVTVKQGTISLGEALSGTFEITALTPRFLDLWSELTGSDELQALRAPD
HHHHHHCCCCCCCEEEECCCCHHHHHCCCCEEEEEECHHHHHHHHHHCCCHHHHHHCCCC
QKEARAAFQHNYHILDVIGRFPAPGIDAARLVAGLRPLQPRLYSIASSLAAAPDEAHLTV
HHHHHHHHHCCCCCEEHHHCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEH
STVRYTLHDLPRTGVASGYLAARTEADATVPVYIQSNDHFHLPDDDVPILMIGAGTGVAP
HHHHHHHHHCCCCCCCCCCEEECCCCCCEEEEEEECCCEEECCCCCCCEEEEECCCCCHH
YRAFMQEREASGASGRSWLFFGERNFRSDFLYQVEWQELLKNGVLSRLDLAFSRDAAPKT
HHHHHHHHHCCCCCCCEEEEECCCCCCCHHEEEEHHHHHHHHCHHHHHHHHHCCCCCCHH
YVQDRLRRQGRDVYAWLEEGAYVYVCGDSAHMAPDVHAALTEIVAEHGGLDREEAGAYLS
HHHHHHHHCCCCEEEHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
ALQRDRRYRLDVY
HHHHCCCEECCCC
>Mature Secondary Structure 
TKLEFPGSALTTDQWTQVNQLATSLSHEQAIWISGYFAGLSHRARAGAAATEIVELAPP
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCHHHHHHHHCCCC
SQVGADGAAATRLLTVLFASETGNSKTLAKALVEKAAAQGIQARLADMAEYKTRGLKDEQ
CCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DLLVITSTHGEGDAPQTAVGFFEFLESRKAPKLPQLRYAVLALGDSTYEKYCEAGKRVDR
CEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCHHEEEEEEECCHHHHHHHHHHHHHHH
RLEELGAQRLADRVDCDVDYEDAADAWIASVVTKLAPAAQAPASAPGASAATMRSGAPSP
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHCCCCCCCCCCHHHHCCCCCCC
ATFDKKNPFQAAVIDNIVLTGRGSTKETRHIELSLADSGLTYQPGDALGVVPRNDPALVE
CCCCCCCCCHHHHHCCEEEECCCCCCCCEEEEEEEECCCCEECCCCEEEECCCCCHHHHH
AILEKLSLSADAPVTVKQGTISLGEALSGTFEITALTPRFLDLWSELTGSDELQALRAPD
HHHHHHCCCCCCCEEEECCCCHHHHHCCCCEEEEEECHHHHHHHHHHCCCHHHHHHCCCC
QKEARAAFQHNYHILDVIGRFPAPGIDAARLVAGLRPLQPRLYSIASSLAAAPDEAHLTV
HHHHHHHHHCCCCCEEHHHCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEH
STVRYTLHDLPRTGVASGYLAARTEADATVPVYIQSNDHFHLPDDDVPILMIGAGTGVAP
HHHHHHHHHCCCCCCCCCCEEECCCCCCEEEEEEECCCEEECCCCCCCEEEEECCCCCHH
YRAFMQEREASGASGRSWLFFGERNFRSDFLYQVEWQELLKNGVLSRLDLAFSRDAAPKT
HHHHHHHHHCCCCCCCEEEEECCCCCCCHHEEEEHHHHHHHHCHHHHHHHHHCCCCCCHH
YVQDRLRRQGRDVYAWLEEGAYVYVCGDSAHMAPDVHAALTEIVAEHGGLDREEAGAYLS
HHHHHHHHCCCCEEEHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
ALQRDRRYRLDVY
HHHHCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]