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The map label for this gene is cyaD [H]

Identifier: 75674734

GI number: 75674734

Start: 598641

End: 599987

Strand: Direct

Name: cyaD [H]

Synonym: Nwi_0536

Alternate gene names: 75674734

Gene position: 598641-599987 (Clockwise)

Preceding gene: 75674733

Following gene: 75674735

Centisome position: 17.6

GC content: 59.84

Gene sequence:

>1347_bases
TTGTCTCCTAGCCAGGCCTCGCTCGCGACCGTTCGCCAGTTCCAGTCGGAGACCGACGCAATCCGGGAGGCGGCGGAGCC
GCTGGTCGCGCGCGTCACCCTGTTCGTATTGTCCGCATTCCTGGTTTCGATCGTCGCAATTCTGTGTCTGACGCAGGTCG
ATCGCGTCATCACCAGCTTGAGCGGAAATATCGTCCCTGTCGGTCATGTCAATGTCTTGCAGGCGCTCGATCCATCGATC
ATCAAGACGATCGACGTGCGCGAGGGCGAGCAGGTGAAGACCGGGCAGTTGCTCGCGACGCTCGATGCCACTTTCACCTC
GGCGGATCTGACGCAGGCGAAGTTGCAGATCGCGAGCCTGGAAGCGCAGGCGGCGCGCGATGAGGCGGAGTTGAAACAGC
AGCCGCTGGTGTTTCCTGATAATCCGGATTCCGACTTCCAGAAGTACGCCGCTTTGCAGAAGGCGCTCTATGAACAGCGC
GTGGCTCAGTATCAGGCCCAGATCAACAGCTTCGAATCAAAGATCAGGGAAACCAAGGCGACGATCGAGAAACTTCGCGC
TGATGATACCCGCTATCGGCAGCGCGAGGAGATCATGGCGAAGATCGAGCAGATGCGCACGACGCTTGCCGAGCGCGGCA
CCGGATCCCAGCTCAACATGTACATCTCCCAGGACGCCAGGCTGGAGTTGCTGCGGACGCTGGAGAATGCGCACAACGGC
CTGATCGAGGCGCAGAACACACTCGGATCGATCACCGCCGATCGCGATGCATTCAAGCAGCAGTGGTTCGCGCAACTGAG
CCAGGACCTGGTGACGACGCGCAACAAGCTGGATGAAGCGCGGGCAACTTATGAGAAAGCTTTCAAGCACCAGGATCTCG
TGCGCTGGACGGCTGCCGAACCATCCGTGGTGCTGACAATGGCCAAGCTTTCGGTCGGATCAGTGCTCAAACCGGGCGAT
CCCTTCATTACGCTCATGCCGATCGACACCAAGCTTGAAGCGGAAATCAGGATCTCCTCGCGGGATGTGGGATTCATCCG
GGCAGGCGACCCCTGCGTGATGAAGATCGATGCTTTCAACGCGGCGGAGCATGGCACGGCGCATGGCAAGGTGCGCTGGA
TCAGCGAGGGCACATTCACGACCGACGATGACGGCAAGCCGGTCGATGCCTATTACAAGGCGAGATGCTCGGTGGACGCG
TCCGCCTTCAAGGGCGTGCCGAGCAATTTTCGTCTCATTCCCGGCATGACCCTGCAAGGAGATATCAACGTCGGGACCCG
TTCTGTGGCGATGTATCTGCTCGGCGGAATGCTGAAAGGCATGAGCGAGGCCATGCGGGAGCCATGA

Upstream 100 bases:

>100_bases
CTGGCGCGGAACGATATCTACAGTTCGCTCTGGTACCAGCAGAATACGCATCTCGCGCCGGCGGTCCGCAACGATAAGCC
GAAACTCCGGAGTCCGACCC

Downstream 100 bases:

>100_bases
TGCTTTCATCCAGATCGCTTCTCCAGTGGGTTCGTCGTCCGGTTCTTGGGCCTTCTCGCGGTGGCCTCTTCGAGCATGGA
ATGGGCGCGTATGAGCGCGG

Product: type I secretion membrane fusion protein, HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MSPSQASLATVRQFQSETDAIREAAEPLVARVTLFVLSAFLVSIVAILCLTQVDRVITSLSGNIVPVGHVNVLQALDPSI
IKTIDVREGEQVKTGQLLATLDATFTSADLTQAKLQIASLEAQAARDEAELKQQPLVFPDNPDSDFQKYAALQKALYEQR
VAQYQAQINSFESKIRETKATIEKLRADDTRYRQREEIMAKIEQMRTTLAERGTGSQLNMYISQDARLELLRTLENAHNG
LIEAQNTLGSITADRDAFKQQWFAQLSQDLVTTRNKLDEARATYEKAFKHQDLVRWTAAEPSVVLTMAKLSVGSVLKPGD
PFITLMPIDTKLEAEIRISSRDVGFIRAGDPCVMKIDAFNAAEHGTAHGKVRWISEGTFTTDDDGKPVDAYYKARCSVDA
SAFKGVPSNFRLIPGMTLQGDINVGTRSVAMYLLGGMLKGMSEAMREP

Sequences:

>Translated_448_residues
MSPSQASLATVRQFQSETDAIREAAEPLVARVTLFVLSAFLVSIVAILCLTQVDRVITSLSGNIVPVGHVNVLQALDPSI
IKTIDVREGEQVKTGQLLATLDATFTSADLTQAKLQIASLEAQAARDEAELKQQPLVFPDNPDSDFQKYAALQKALYEQR
VAQYQAQINSFESKIRETKATIEKLRADDTRYRQREEIMAKIEQMRTTLAERGTGSQLNMYISQDARLELLRTLENAHNG
LIEAQNTLGSITADRDAFKQQWFAQLSQDLVTTRNKLDEARATYEKAFKHQDLVRWTAAEPSVVLTMAKLSVGSVLKPGD
PFITLMPIDTKLEAEIRISSRDVGFIRAGDPCVMKIDAFNAAEHGTAHGKVRWISEGTFTTDDDGKPVDAYYKARCSVDA
SAFKGVPSNFRLIPGMTLQGDINVGTRSVAMYLLGGMLKGMSEAMREP
>Mature_447_residues
SPSQASLATVRQFQSETDAIREAAEPLVARVTLFVLSAFLVSIVAILCLTQVDRVITSLSGNIVPVGHVNVLQALDPSII
KTIDVREGEQVKTGQLLATLDATFTSADLTQAKLQIASLEAQAARDEAELKQQPLVFPDNPDSDFQKYAALQKALYEQRV
AQYQAQINSFESKIRETKATIEKLRADDTRYRQREEIMAKIEQMRTTLAERGTGSQLNMYISQDARLELLRTLENAHNGL
IEAQNTLGSITADRDAFKQQWFAQLSQDLVTTRNKLDEARATYEKAFKHQDLVRWTAAEPSVVLTMAKLSVGSVLKPGDP
FITLMPIDTKLEAEIRISSRDVGFIRAGDPCVMKIDAFNAAEHGTAHGKVRWISEGTFTTDDDGKPVDAYYKARCSVDAS
AFKGVPSNFRLIPGMTLQGDINVGTRSVAMYLLGGMLKGMSEAMREP

Specific function: CyaD is necessary for transport of calmodulin-sensitive adenylate cyclase-hemolysin (cyclolysin) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003997
- InterPro:   IPR006143
- InterPro:   IPR006144
- InterPro:   IPR010129 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 49464; Mature: 49333

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPSQASLATVRQFQSETDAIREAAEPLVARVTLFVLSAFLVSIVAILCLTQVDRVITSL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGNIVPVGHVNVLQALDPSIIKTIDVREGEQVKTGQLLATLDATFTSADLTQAKLQIASL
CCCEEEECCHHHHHHCCHHHHEEEECCCCCCCCCCCEEEEHHHHHCCCHHHHHHHHHHHH
EAQAARDEAELKQQPLVFPDNPDSDFQKYAALQKALYEQRVAQYQAQINSFESKIRETKA
HHHHHHCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIEKLRADDTRYRQREEIMAKIEQMRTTLAERGTGSQLNMYISQDARLELLRTLENAHNG
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCC
LIEAQNTLGSITADRDAFKQQWFAQLSQDLVTTRNKLDEARATYEKAFKHQDLVRWTAAE
CEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
PSVVLTMAKLSVGSVLKPGDPFITLMPIDTKLEAEIRISSRDVGFIRAGDPCVMKIDAFN
CCEEEEEHHHHHCCCCCCCCCEEEEEECCCCEEEEEEEECCCEEEEECCCCEEEEEECCC
AAEHGTAHGKVRWISEGTFTTDDDGKPVDAYYKARCSVDASAFKGVPSNFRLIPGMTLQG
CCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHCCCCHHHHCCCCCCCEECCCEEEEC
DINVGTRSVAMYLLGGMLKGMSEAMREP
CCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SPSQASLATVRQFQSETDAIREAAEPLVARVTLFVLSAFLVSIVAILCLTQVDRVITSL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGNIVPVGHVNVLQALDPSIIKTIDVREGEQVKTGQLLATLDATFTSADLTQAKLQIASL
CCCEEEECCHHHHHHCCHHHHEEEECCCCCCCCCCCEEEEHHHHHCCCHHHHHHHHHHHH
EAQAARDEAELKQQPLVFPDNPDSDFQKYAALQKALYEQRVAQYQAQINSFESKIRETKA
HHHHHHCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIEKLRADDTRYRQREEIMAKIEQMRTTLAERGTGSQLNMYISQDARLELLRTLENAHNG
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCC
LIEAQNTLGSITADRDAFKQQWFAQLSQDLVTTRNKLDEARATYEKAFKHQDLVRWTAAE
CEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
PSVVLTMAKLSVGSVLKPGDPFITLMPIDTKLEAEIRISSRDVGFIRAGDPCVMKIDAFN
CCEEEEEHHHHHCCCCCCCCCEEEEEECCCCEEEEEEEECCCEEEEECCCCEEEEEECCC
AAEHGTAHGKVRWISEGTFTTDDDGKPVDAYYKARCSVDASAFKGVPSNFRLIPGMTLQG
CCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHCCCCHHHHCCCCCCCEECCCEEEEC
DINVGTRSVAMYLLGGMLKGMSEAMREP
CCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2905265; 12910271 [H]