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The map label for this gene is phoB [H]

Identifier: 75674709

GI number: 75674709

Start: 568189

End: 568893

Strand: Direct

Name: phoB [H]

Synonym: Nwi_0511

Alternate gene names: 75674709

Gene position: 568189-568893 (Clockwise)

Preceding gene: 75674708

Following gene: 162139850

Centisome position: 16.7

GC content: 65.53

Gene sequence:

>705_bases
ATGGGTGCACGCATTCTGGTAGTTGAAGACGAGGAAGCGTTGACGACGCTGCTGCGCTACAATCTTGAAGCGGAAGGGTA
CGAGGTCGAAACGGTCGTGCGGGGTGACGAGGCGGATACGCGGCTGAAAGAGCGCGTTCCCGACCTTGTGGTGCTGGACT
GGATGCTGCCGGGCCTGTCCGGCATCGAGCTGTGCCGCCGGCTGCGGGCCCGCTCCGACACCAGGCAGCTTCCGATCATC
ATGCTCACCGCGCGCGGCGAGGAGAGCGAGCGGGTGCGGGGGCTCGCGACCGGCGCGGACGACTACGTGGTCAAGCCGTT
CTCGGTGCCGGAACTCCTGGCGAGGGTGAAAAGCCTGCTGCGGCGCGCGAGCCCGGCGCGGCTGGCGTCTGTCCTGAGCT
TTGGCGACATAGAACTCGACCGCGACCGGCACCGTGTCGTGCGCTCCGGCCGTCCGATCGATCTTGGCCCGACCGAGTAT
CGGTTGCTGGAGTTTTTTCTCGAGCATCCCGGCCGGGTGTTCAGCCGCGAGCAATTGCTCGACAGCGTCTGGGGCCGTGA
TGTCTATATCGACGAGCGCACCGTCGACGTTCACATCGGTCGGCTGCGCAAGCTGCTCAATCCTGACGGCGAGCTGGATC
CGATCCGCACGGTGCGCGGCGCGGGTTACGCACTTGACGAGCAGTTCGCAAGCAGGACCGAGTAA

Upstream 100 bases:

>100_bases
GATCGAGGGTCAGCAGATCCTCGACAAGCGTCCCAAGGGGGACATGACAGGTTTCGCATCGGCGGCTCCGGGAAACTGAA
ACGGGATCAGGCGAGACACG

Downstream 100 bases:

>100_bases
GCGGGGCGAGTTGAGGGAGCGTCGGTGATCCTCGTTGTTCTGCACCAAAGCGCCTGCCGCGGCCGGAACCGTTCCGTTTC
TGACGGAGTCGTGAGCGGGG

Product: phosphate regulon transcriptional regulatory protein phoB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MGARILVVEDEEALTTLLRYNLEAEGYEVETVVRGDEADTRLKERVPDLVVLDWMLPGLSGIELCRRLRARSDTRQLPII
MLTARGEESERVRGLATGADDYVVKPFSVPELLARVKSLLRRASPARLASVLSFGDIELDRDRHRVVRSGRPIDLGPTEY
RLLEFFLEHPGRVFSREQLLDSVWGRDVYIDERTVDVHIGRLRKLLNPDGELDPIRTVRGAGYALDEQFASRTE

Sequences:

>Translated_234_residues
MGARILVVEDEEALTTLLRYNLEAEGYEVETVVRGDEADTRLKERVPDLVVLDWMLPGLSGIELCRRLRARSDTRQLPII
MLTARGEESERVRGLATGADDYVVKPFSVPELLARVKSLLRRASPARLASVLSFGDIELDRDRHRVVRSGRPIDLGPTEY
RLLEFFLEHPGRVFSREQLLDSVWGRDVYIDERTVDVHIGRLRKLLNPDGELDPIRTVRGAGYALDEQFASRTE
>Mature_233_residues
GARILVVEDEEALTTLLRYNLEAEGYEVETVVRGDEADTRLKERVPDLVVLDWMLPGLSGIELCRRLRARSDTRQLPIIM
LTARGEESERVRGLATGADDYVVKPFSVPELLARVKSLLRRASPARLASVLSFGDIELDRDRHRVVRSGRPIDLGPTEYR
LLEFFLEHPGRVFSREQLLDSVWGRDVYIDERTVDVHIGRLRKLLNPDGELDPIRTVRGAGYALDEQFASRTE

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=229, Percent_Identity=51.0917030567686, Blast_Score=240, Evalue=6e-65,
Organism=Escherichia coli, GI1790860, Length=227, Percent_Identity=44.9339207048458, Blast_Score=168, Evalue=3e-43,
Organism=Escherichia coli, GI1788394, Length=222, Percent_Identity=43.6936936936937, Blast_Score=164, Evalue=5e-42,
Organism=Escherichia coli, GI1789809, Length=233, Percent_Identity=39.0557939914163, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI1786784, Length=223, Percent_Identity=39.9103139013453, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1786911, Length=229, Percent_Identity=39.3013100436681, Blast_Score=133, Evalue=8e-33,
Organism=Escherichia coli, GI1787375, Length=220, Percent_Identity=35.9090909090909, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1789402, Length=225, Percent_Identity=34.6666666666667, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=36.7713004484305, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1787229, Length=232, Percent_Identity=38.3620689655172, Blast_Score=123, Evalue=8e-30,
Organism=Escherichia coli, GI2367329, Length=230, Percent_Identity=38.2608695652174, Blast_Score=121, Evalue=3e-29,
Organism=Escherichia coli, GI1790863, Length=230, Percent_Identity=36.5217391304348, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1790552, Length=222, Percent_Identity=36.9369369369369, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI145693140, Length=231, Percent_Identity=33.7662337662338, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788550, Length=121, Percent_Identity=33.8842975206612, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788191, Length=120, Percent_Identity=29.1666666666667, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1790299, Length=113, Percent_Identity=30.0884955752212, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26636; Mature: 26505

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGARILVVEDEEALTTLLRYNLEAEGYEVETVVRGDEADTRLKERVPDLVVLDWMLPGLS
CCCEEEEEECHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHCCCEEEEHHHHCCCC
GIELCRRLRARSDTRQLPIIMLTARGEESERVRGLATGADDYVVKPFSVPELLARVKSLL
HHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHH
RRASPARLASVLSFGDIELDRDRHRVVRSGRPIDLGPTEYRLLEFFLEHPGRVFSREQLL
HHCCHHHHHHHHHHCCCEECCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
DSVWGRDVYIDERTVDVHIGRLRKLLNPDGELDPIRTVRGAGYALDEQFASRTE
HHHCCCEEEEECCEEEHHHHHHHHHHCCCCCCCHHHHHCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
GARILVVEDEEALTTLLRYNLEAEGYEVETVVRGDEADTRLKERVPDLVVLDWMLPGLS
CCEEEEEECHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHCCCEEEEHHHHCCCC
GIELCRRLRARSDTRQLPIIMLTARGEESERVRGLATGADDYVVKPFSVPELLARVKSLL
HHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHH
RRASPARLASVLSFGDIELDRDRHRVVRSGRPIDLGPTEYRLLEFFLEHPGRVFSREQLL
HHCCHHHHHHHHHHCCCEECCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
DSVWGRDVYIDERTVDVHIGRLRKLLNPDGELDPIRTVRGAGYALDEQFASRTE
HHHCCCEEEEECCEEEHHHHHHHHHHCCCCCCCHHHHHCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]