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The map label for this gene is yfgF [C]

Identifier: 75674698

GI number: 75674698

Start: 555994

End: 557772

Strand: Direct

Name: yfgF [C]

Synonym: Nwi_0500

Alternate gene names: 75674698

Gene position: 555994-557772 (Clockwise)

Preceding gene: 75674696

Following gene: 75674700

Centisome position: 16.34

GC content: 64.47

Gene sequence:

>1779_bases
GTGACATCCGCCATTTCACCGGCAGTCAATCCCTCGTCTGGCGCCGATGCGTCACAAACCATCAGCGCGCCTGCTGGCCT
GCCCCAGGCAACCCAGGAGCCGCGGGTGTCGGCGATCCTCACGGCGCTCGGCCGGGCTGCTTTCGTGTGGGATATCCCGA
GCGACGCGATAAACTGGACCGAGGGAGCTGCGTCGATCTTCAAGGGCATCGACCCGTTCCTGCTCGCGACCGGAGCCCGA
TATGGACGCCTGATCGAACCGTCGGGATCGGTCCGCACCAATATCTTTTCCGCCGCGCCGACCACCCGGCGGCGCTTTGA
TGAGGGAGTGCCCTATTTCATCGAGTATGGCGTGCGCGCCAGCGCCTCCGCGCCGATGCTGTGGATCGAGGAGGCCGGAT
GCTGGTTCGCCGACGCCGATGGCAAGGTGGTGCGCGTCGAAGGCATCGTGCGGCTTGAGAATGAGCGGCGCGCGCGCGAC
GAGCAACTGATCAACCTGTCCCGGAACGATGCCCTCACCGGCGAGCTTAATCGCACCTATCTGACCGCGGCTCTCGCCGA
AGCGATCGAGGAAGCCGCGCGCTACCGCTCGCCGGTCGCCTTCATGATGGTCGGGATCGATCACCTCGCGCGCATCAACA
ATGCTTTCGGCTTCGATGTCGCCGACGAGGTGATCAGCGATGTCGCCAGGCGCATCCGAAGGCGGTTGCGCAGCGGCGAC
ACCCTTGGCCGCTTTTCCGGCAACAGATTCGGGGTGATCCTGAAGAACTGCGGCCTGGATGAGGTGAGCATCGCCGCCGA
ACGCCTTCTGGCGGGCATCCGCGAAGAGATCGTGATGACCAAATCCGGTCCCGTTTCGGTCACCGCCTCCATAGGCGCCG
TTCTGGCCCCGCGTTATGCGCAGACTGCCGATGAAGCGATCAATCGCGCTCAGGAAATCCTCGAAGTTGCGAAAGGCCGC
CGCTGCGGTTCGCTCGCCGTGTGGCGACCCGATACCGAGCGTGAGGCGCAGCGTCGGATCAACATCCGGGTGACCGACGA
GATCGTCACCGCGCTGAACGAGCGCCGCATCCTCACCGCTTTCGAGCGGGTGGTCGACGCGCGCACGCGCGAAGCGGCGT
TTTATGAGTGCCTGGTGCGGATGCAGCAGCAGAATGGTCAGATCCTGCTCGCGCCCGATGTCGTGCCGGTCGCGGAGAAG
CTTGGCCTGATCCGTCTGGTCGATCATCGCGTGCTCGAGCTTGTGATCGCCGAGCTGGCGGAAGCGCCCCACGTCCAGCT
CAGTCTCAATATCTCGCCGGAAACCACCATGGATGTCGACTGGTGGGCGGCGATCGAAGCTCTGATGGGTGCGCATCCCG
GCGTCGCCGAGCGCCTGATCGTGGAGATCACCGAAACCGCGGCGATTCAGGATATCGACGACGTTCAGCGGTTCGTTACG
CGGTTGAAGAATCTGGGCAGCCGCATCGCGATCGACGATTTCGGCGCCGGCTACACCTCGTTCCGGAACTTGCGCAAGCT
CGGCGTGGACATCGTCAAGATCGACGGCGCGTTCGTGCAGAACCTGGCGCGATCCTCAGACGACCGGGCCTTCGTGCAGA
CGCTGATCGATCTTGCGCGGCGACTCGATATCAGAACGGTCGCCGAGTGGGTTCAGGACGAGCAATCAGCGGCCATGCTG
CGGGACTGGGGCTGCGACTTCATCCAGGGCAGGCTGATCGGCCTTGCTTCGCCATCGCGCCCGTGGAGCGGTCCGGCGGC
CGTTCCGGCAACGAACTGA

Upstream 100 bases:

>100_bases
GGTTTTTCGGATTGTTCACGAACTTTACCTAGCGTGAACGGGTTCGAGCACCCTATTCTTCAGAAGGGAATCACTTGCAG
CGTCCCATAATGAGGCGACC

Downstream 100 bases:

>100_bases
AGTTCTTCTTCATAACAGACACCCGTCTTCGCCGCTTTTGCCGGGATGACCGGAAAATGCGCAGCGATGATGAGTGACGA
GACGGCGTGAACTCAGGCGA

Product: diguanylate cyclase/phophodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 592; Mature: 591

Protein sequence:

>592_residues
MTSAISPAVNPSSGADASQTISAPAGLPQATQEPRVSAILTALGRAAFVWDIPSDAINWTEGAASIFKGIDPFLLATGAR
YGRLIEPSGSVRTNIFSAAPTTRRRFDEGVPYFIEYGVRASASAPMLWIEEAGCWFADADGKVVRVEGIVRLENERRARD
EQLINLSRNDALTGELNRTYLTAALAEAIEEAARYRSPVAFMMVGIDHLARINNAFGFDVADEVISDVARRIRRRLRSGD
TLGRFSGNRFGVILKNCGLDEVSIAAERLLAGIREEIVMTKSGPVSVTASIGAVLAPRYAQTADEAINRAQEILEVAKGR
RCGSLAVWRPDTEREAQRRINIRVTDEIVTALNERRILTAFERVVDARTREAAFYECLVRMQQQNGQILLAPDVVPVAEK
LGLIRLVDHRVLELVIAELAEAPHVQLSLNISPETTMDVDWWAAIEALMGAHPGVAERLIVEITETAAIQDIDDVQRFVT
RLKNLGSRIAIDDFGAGYTSFRNLRKLGVDIVKIDGAFVQNLARSSDDRAFVQTLIDLARRLDIRTVAEWVQDEQSAAML
RDWGCDFIQGRLIGLASPSRPWSGPAAVPATN

Sequences:

>Translated_592_residues
MTSAISPAVNPSSGADASQTISAPAGLPQATQEPRVSAILTALGRAAFVWDIPSDAINWTEGAASIFKGIDPFLLATGAR
YGRLIEPSGSVRTNIFSAAPTTRRRFDEGVPYFIEYGVRASASAPMLWIEEAGCWFADADGKVVRVEGIVRLENERRARD
EQLINLSRNDALTGELNRTYLTAALAEAIEEAARYRSPVAFMMVGIDHLARINNAFGFDVADEVISDVARRIRRRLRSGD
TLGRFSGNRFGVILKNCGLDEVSIAAERLLAGIREEIVMTKSGPVSVTASIGAVLAPRYAQTADEAINRAQEILEVAKGR
RCGSLAVWRPDTEREAQRRINIRVTDEIVTALNERRILTAFERVVDARTREAAFYECLVRMQQQNGQILLAPDVVPVAEK
LGLIRLVDHRVLELVIAELAEAPHVQLSLNISPETTMDVDWWAAIEALMGAHPGVAERLIVEITETAAIQDIDDVQRFVT
RLKNLGSRIAIDDFGAGYTSFRNLRKLGVDIVKIDGAFVQNLARSSDDRAFVQTLIDLARRLDIRTVAEWVQDEQSAAML
RDWGCDFIQGRLIGLASPSRPWSGPAAVPATN
>Mature_591_residues
TSAISPAVNPSSGADASQTISAPAGLPQATQEPRVSAILTALGRAAFVWDIPSDAINWTEGAASIFKGIDPFLLATGARY
GRLIEPSGSVRTNIFSAAPTTRRRFDEGVPYFIEYGVRASASAPMLWIEEAGCWFADADGKVVRVEGIVRLENERRARDE
QLINLSRNDALTGELNRTYLTAALAEAIEEAARYRSPVAFMMVGIDHLARINNAFGFDVADEVISDVARRIRRRLRSGDT
LGRFSGNRFGVILKNCGLDEVSIAAERLLAGIREEIVMTKSGPVSVTASIGAVLAPRYAQTADEAINRAQEILEVAKGRR
CGSLAVWRPDTEREAQRRINIRVTDEIVTALNERRILTAFERVVDARTREAAFYECLVRMQQQNGQILLAPDVVPVAEKL
GLIRLVDHRVLELVIAELAEAPHVQLSLNISPETTMDVDWWAAIEALMGAHPGVAERLIVEITETAAIQDIDDVQRFVTR
LKNLGSRIAIDDFGAGYTSFRNLRKLGVDIVKIDGAFVQNLARSSDDRAFVQTLIDLARRLDIRTVAEWVQDEQSAAMLR
DWGCDFIQGRLIGLASPSRPWSGPAAVPATN

Specific function: Unknown

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 MHYT domain [H]

Homologues:

Organism=Escherichia coli, GI1788849, Length=214, Percent_Identity=33.6448598130841, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1787541, Length=436, Percent_Identity=24.7706422018349, Blast_Score=127, Evalue=3e-30,
Organism=Escherichia coli, GI87081921, Length=440, Percent_Identity=24.5454545454545, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI87082096, Length=268, Percent_Identity=30.2238805970149, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1790496, Length=234, Percent_Identity=29.4871794871795, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1788502, Length=246, Percent_Identity=28.4552845528455, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI226510982, Length=419, Percent_Identity=25.5369928400955, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI87081980, Length=245, Percent_Identity=29.3877551020408, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786507, Length=261, Percent_Identity=26.0536398467433, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788381, Length=503, Percent_Identity=21.8687872763419, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI87081743, Length=237, Percent_Identity=29.535864978903, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI87081845, Length=232, Percent_Identity=28.448275862069, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1787055, Length=249, Percent_Identity=25.3012048192771, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787262, Length=160, Percent_Identity=31.875, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1787410, Length=231, Percent_Identity=27.2727272727273, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1786584, Length=167, Percent_Identity=28.1437125748503, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI145693134, Length=167, Percent_Identity=27.5449101796407, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87082007, Length=122, Percent_Identity=34.4262295081967, Blast_Score=67, Evalue=5e-12,
Organism=Escherichia coli, GI1789650, Length=388, Percent_Identity=19.5876288659794, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR005330 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF03707 MHYT [H]

EC number: NA

Molecular weight: Translated: 64927; Mature: 64795

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSAISPAVNPSSGADASQTISAPAGLPQATQEPRVSAILTALGRAAFVWDIPSDAINWT
CCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEECCCCCCCCC
EGAASIFKGIDPFLLATGARYGRLIEPSGSVRTNIFSAAPTTRRRFDEGVPYFIEYGVRA
HHHHHHHHCCCHHHHHCCCCCCEEECCCCCCCHHEEECCCHHHHHHHCCCCCEEECCCCC
SASAPMLWIEEAGCWFADADGKVVRVEGIVRLENERRARDEQLINLSRNDALTGELNRTY
CCCCCEEEEECCCCEEECCCCCEEEEEEEEEECCHHHHHHHHHHCCCCCCCCCCCCHHHH
LTAALAEAIEEAARYRSPVAFMMVGIDHLARINNAFGFDVADEVISDVARRIRRRLRSGD
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
TLGRFSGNRFGVILKNCGLDEVSIAAERLLAGIREEIVMTKSGPVSVTASIGAVLAPRYA
CCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHEEECCCCEEEEEHHHHHHCCHHH
QTADEAINRAQEILEVAKGRRCGSLAVWRPDTEREAQRRINIRVTDEIVTALNERRILTA
HHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHEEEEEHHHHHHHHHCCHHHHH
FERVVDARTREAAFYECLVRMQQQNGQILLAPDVVPVAEKLGLIRLVDHRVLELVIAELA
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHCCHHHHHHHHHHHHHHHHHH
EAPHVQLSLNISPETTMDVDWWAAIEALMGAHPGVAERLIVEITETAAIQDIDDVQRFVT
CCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
RLKNLGSRIAIDDFGAGYTSFRNLRKLGVDIVKIDGAFVQNLARSSDDRAFVQTLIDLAR
HHHHCCCEEEEECCCCCHHHHHHHHHHCCEEEEECHHHHHHHHHCCCHHHHHHHHHHHHH
RLDIRTVAEWVQDEQSAAMLRDWGCDFIQGRLIGLASPSRPWSGPAAVPATN
HCCHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TSAISPAVNPSSGADASQTISAPAGLPQATQEPRVSAILTALGRAAFVWDIPSDAINWT
CCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEECCCCCCCCC
EGAASIFKGIDPFLLATGARYGRLIEPSGSVRTNIFSAAPTTRRRFDEGVPYFIEYGVRA
HHHHHHHHCCCHHHHHCCCCCCEEECCCCCCCHHEEECCCHHHHHHHCCCCCEEECCCCC
SASAPMLWIEEAGCWFADADGKVVRVEGIVRLENERRARDEQLINLSRNDALTGELNRTY
CCCCCEEEEECCCCEEECCCCCEEEEEEEEEECCHHHHHHHHHHCCCCCCCCCCCCHHHH
LTAALAEAIEEAARYRSPVAFMMVGIDHLARINNAFGFDVADEVISDVARRIRRRLRSGD
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
TLGRFSGNRFGVILKNCGLDEVSIAAERLLAGIREEIVMTKSGPVSVTASIGAVLAPRYA
CCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHEEECCCCEEEEEHHHHHHCCHHH
QTADEAINRAQEILEVAKGRRCGSLAVWRPDTEREAQRRINIRVTDEIVTALNERRILTA
HHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHEEEEEHHHHHHHHHCCHHHHH
FERVVDARTREAAFYECLVRMQQQNGQILLAPDVVPVAEKLGLIRLVDHRVLELVIAELA
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHCCHHHHHHHHHHHHHHHHHH
EAPHVQLSLNISPETTMDVDWWAAIEALMGAHPGVAERLIVEITETAAIQDIDDVQRFVT
CCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
RLKNLGSRIAIDDFGAGYTSFRNLRKLGVDIVKIDGAFVQNLARSSDDRAFVQTLIDLAR
HHHHCCCEEEEECCCCCHHHHHHHHHHCCEEEEECHHHHHHHHHCCCHHHHHHHHHHHHH
RLDIRTVAEWVQDEQSAAMLRDWGCDFIQGRLIGLASPSRPWSGPAAVPATN
HCCHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]