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The map label for this gene is aroK

Identifier: 75674593

GI number: 75674593

Start: 442655

End: 443299

Strand: Direct

Name: aroK

Synonym: Nwi_0395

Alternate gene names: 75674593

Gene position: 442655-443299 (Clockwise)

Preceding gene: 75674588

Following gene: 75674594

Centisome position: 13.01

GC content: 65.74

Gene sequence:

>645_bases
TTGACGTCCGAAACCGTTTCACCGTCCAGCTTCGGAGCATCCCCCGAGACCGCCGTTGTCGCGGCGCTCGCGGGGCGGTC
GCTGGCGCTGGTCGGCATGATGGGAGCCGGCAAGTCCACCATCGGACGCCGGCTGGCGGCGCGCCTCAGGATGCGATTCG
TCGATGCCGACGCCGAAATCGAACTCGCGGCCGGAATGCCGATTCCCGAAATCTTCGAAACCCACGGCGAGCCCCATTTC
CGGGATGGCGAGGCGCGCGTGATCGCACGGTTGCTGAATGGCGGCCCGATCGTGCTCGCAACAGGCGGCGGCGCCGTGCT
GCGCGAGGAGACCCGGGCACGCCTCTCAGAACGGGCGGTCTCGATCTGGCTGAAGGCGGACGCCGAGGTGATTCTGCGCC
GGGTCAGGCGCCGCGCCGACAGACCCTTGCTGCAAACGGCCGATCCCGCCGCGACGATCAAGCGCCTTATCGCCGAGCGC
GAGCCGATCTATCGACAGGCGGATATCACGGTCGCCTCGCGCGACGTGCCGTATGAATGGATCGTCGATGAATGCATCGG
CCTGCTTCATCGCAGGCTTTGCCGCGGCGAAATGACTCGAAATGCGATGAAAGACCCTGACGAGGGATTTGACACCACGC
AATGA

Upstream 100 bases:

>100_bases
TCGTCGTGAGCTTTGGATCTGACATTCTCCGTCGGAAATCGCGAAAGGTCCGAATGTCGAATCCGTTTCGGGCGCGCAAC
CATGTTCCGGCAGGTGACCC

Downstream 100 bases:

>100_bases
CCATTCCGATCAAAGAATCAGACCCGATCACGGTCGATGTCGCGCTCGGTGAGCGCTCCTACGACATTGTCATCGGACGA
GGCGTCCTGCCGTCGCTGGG

Product: shikimate kinase

Products: NA

Alternate protein names: SK

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MTSETVSPSSFGASPETAVVAALAGRSLALVGMMGAGKSTIGRRLAARLRMRFVDADAEIELAAGMPIPEIFETHGEPHF
RDGEARVIARLLNGGPIVLATGGGAVLREETRARLSERAVSIWLKADAEVILRRVRRRADRPLLQTADPAATIKRLIAER
EPIYRQADITVASRDVPYEWIVDECIGLLHRRLCRGEMTRNAMKDPDEGFDTTQ

Sequences:

>Translated_214_residues
MTSETVSPSSFGASPETAVVAALAGRSLALVGMMGAGKSTIGRRLAARLRMRFVDADAEIELAAGMPIPEIFETHGEPHF
RDGEARVIARLLNGGPIVLATGGGAVLREETRARLSERAVSIWLKADAEVILRRVRRRADRPLLQTADPAATIKRLIAER
EPIYRQADITVASRDVPYEWIVDECIGLLHRRLCRGEMTRNAMKDPDEGFDTTQ
>Mature_213_residues
TSETVSPSSFGASPETAVVAALAGRSLALVGMMGAGKSTIGRRLAARLRMRFVDADAEIELAAGMPIPEIFETHGEPHFR
DGEARVIARLLNGGPIVLATGGGAVLREETRARLSERAVSIWLKADAEVILRRVRRRADRPLLQTADPAATIKRLIAERE
PIYRQADITVASRDVPYEWIVDECIGLLHRRLCRGEMTRNAMKDPDEGFDTTQ

Specific function: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate

COG id: COG0703

COG function: function code E; Shikimate kinase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate kinase family

Homologues:

Organism=Escherichia coli, GI87082255, Length=167, Percent_Identity=43.7125748502994, Blast_Score=127, Evalue=5e-31,
Organism=Escherichia coli, GI1786587, Length=160, Percent_Identity=29.375, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): AROK_NITWN (Q3SVM9)

Other databases:

- EMBL:   CP000115
- RefSeq:   YP_317014.1
- ProteinModelPortal:   Q3SVM9
- SMR:   Q3SVM9
- STRING:   Q3SVM9
- GeneID:   3676980
- GenomeReviews:   CP000115_GR
- KEGG:   nwi:Nwi_0395
- NMPDR:   fig|323098.3.peg.566
- eggNOG:   COG0703
- HOGENOM:   HBG335815
- OMA:   VIYLRAN
- PhylomeDB:   Q3SVM9
- ProtClustDB:   PRK13946
- BioCyc:   NWIN323098:NWI_0395-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00109
- InterPro:   IPR000623
- InterPro:   IPR023000
- PRINTS:   PR01100

Pfam domain/function: PF01202 SKI

EC number: =2.7.1.71

Molecular weight: Translated: 23443; Mature: 23312

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS01128 SHIKIMATE_KINASE

Important sites: BINDING 57-57 BINDING 81-81 BINDING 103-103 BINDING 141-141 BINDING 160-160

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSETVSPSSFGASPETAVVAALAGRSLALVGMMGAGKSTIGRRLAARLRMRFVDADAEI
CCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCE
ELAAGMPIPEIFETHGEPHFRDGEARVIARLLNGGPIVLATGGGAVLREETRARLSERAV
EEECCCCCHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHH
SIWLKADAEVILRRVRRRADRPLLQTADPAATIKRLIAEREPIYRQADITVASRDVPYEW
HHHHHCCHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHH
IVDECIGLLHRRLCRGEMTRNAMKDPDEGFDTTQ
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TSETVSPSSFGASPETAVVAALAGRSLALVGMMGAGKSTIGRRLAARLRMRFVDADAEI
CCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCE
ELAAGMPIPEIFETHGEPHFRDGEARVIARLLNGGPIVLATGGGAVLREETRARLSERAV
EEECCCCCHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHH
SIWLKADAEVILRRVRRRADRPLLQTADPAATIKRLIAEREPIYRQADITVASRDVPYEW
HHHHHCCHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHH
IVDECIGLLHRRLCRGEMTRNAMKDPDEGFDTTQ
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA