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The map label for this gene is fadA [H]

Identifier: 75674477

GI number: 75674477

Start: 313329

End: 314516

Strand: Direct

Name: fadA [H]

Synonym: Nwi_0279

Alternate gene names: 75674477

Gene position: 313329-314516 (Clockwise)

Preceding gene: 75674476

Following gene: 75674479

Centisome position: 9.21

GC content: 66.75

Gene sequence:

>1188_bases
ATGACCGAAGCCGTGATTGTCTCCACCGCCCGCACGCCGATCGGCAAGGCCTATCGGGGCGCTCTCAACGCCACTGAAGG
AGCGACCCTGCTCGGCCATGCCATCGGAGAAGCCGCTGTCCGCGCCGGGGTTGATCCACAGGAGATCGAGGACGTGGTGA
TGGGCGTGGCCATGCAGCAGGGCGCCACTGGCGGCAACATCGCCCGCAAGGCGCTGCTTCGCGCCGGGCTCCCCGTGACC
GTCGCCGGCGCCACCATCGACCGCCAATGCGCCTCGGGCCTTCAGGCGATCGCGCTGGCCGCGCGTTCCGTCCTGTTCGA
CGGCGTCGGAATCGCGGTCGGCGGCGGCGGCGAGTCGATCAGCCTGGTGCAGAACGAGCACGCCAACACCTTTCACGCAG
AGGATCCGGCGCTTCTGGAGATCAAGCGCGAGGTCTACATGCCCATGATCGACACCGCGGAAGTGGTCGCGAAGCGCTAC
GGCATCTCGCGCCAGGCGCAGGACGAATATGCGCTCGAAAGCCAGCGCCGCACCGCCGCCGCGCAGCAGGAGGGCCGATT
CAAGGACGAACTGGCGCCGATCTCCACAAGAATGGCGATCGTCGACAAGGAAACCGGGAACGTCACGTTCAGGGACGTGA
CGCTGTCGCAGGACGAAGGTCCGCGTCCCGATACGTCGGCTGAAGGTCTCGCCGGGCTCAAGCCGGTTCGCGGCGAAGGC
TGCACCATCACCGCCGGCAATGCCAGCCAGCTGTCGGACGGCGCGAGCGCGGCCGTGATCATGAGCGGCACGGAGGCGTC
GAGGCGCGGCCTGAATCCGCTCGGCATCTTCCGCGGTTTCGTCTCAGCCGGCTGCGAGCCTGATGAAATGGGCATCGGCC
CGGTGTTCGCGGTGCCGCGCCTTCTGAAACGACACGGCCTCACCGTGGGAGACATCGATCTTTGGGAGTTGAACGAAGCG
TTCGCGGTGCAGGTGATCTATTGCCGCGACAGGCTGGGCATCGATCCCGAAAAACTCAACGTCGACGGCGGAGCGATCGC
GGTCGGTCATCCCTACGGAATGTCGGGCGCGCGCCTTGCCGGTCATGCCCTGATCGAGGGACGCCGGCGCAAGGCGAAAT
ATGCCGTGGTCACCATGTGCGTCGGCGGCGGCATGGGCGCGGCCGGCCTATTCGAGGTGCTTCAGTAA

Upstream 100 bases:

>100_bases
AGAAAACGCGTCAAATCAAATTCATGGAGCCCGCTTCTGATTTTATCAGAAGCGGAAAGGCTCTAAATCAGGATGAACCC
GAATTGCCAGGAGACCGCCG

Downstream 100 bases:

>100_bases
AGAGGCGCTCCGGTAAATTCCGGGATGCGCTCCCGCGCGTCCGACGACGCGTCAGACCAATATCAATAGAGCCCCGCTTC
CAACTCTGTCGGAAGCGGAA

Product: thiolase

Products: NA

Alternate protein names: Acetyl-CoA acyltransferase; Beta-ketothiolase [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MTEAVIVSTARTPIGKAYRGALNATEGATLLGHAIGEAAVRAGVDPQEIEDVVMGVAMQQGATGGNIARKALLRAGLPVT
VAGATIDRQCASGLQAIALAARSVLFDGVGIAVGGGGESISLVQNEHANTFHAEDPALLEIKREVYMPMIDTAEVVAKRY
GISRQAQDEYALESQRRTAAAQQEGRFKDELAPISTRMAIVDKETGNVTFRDVTLSQDEGPRPDTSAEGLAGLKPVRGEG
CTITAGNASQLSDGASAAVIMSGTEASRRGLNPLGIFRGFVSAGCEPDEMGIGPVFAVPRLLKRHGLTVGDIDLWELNEA
FAVQVIYCRDRLGIDPEKLNVDGGAIAVGHPYGMSGARLAGHALIEGRRRKAKYAVVTMCVGGGMGAAGLFEVLQ

Sequences:

>Translated_395_residues
MTEAVIVSTARTPIGKAYRGALNATEGATLLGHAIGEAAVRAGVDPQEIEDVVMGVAMQQGATGGNIARKALLRAGLPVT
VAGATIDRQCASGLQAIALAARSVLFDGVGIAVGGGGESISLVQNEHANTFHAEDPALLEIKREVYMPMIDTAEVVAKRY
GISRQAQDEYALESQRRTAAAQQEGRFKDELAPISTRMAIVDKETGNVTFRDVTLSQDEGPRPDTSAEGLAGLKPVRGEG
CTITAGNASQLSDGASAAVIMSGTEASRRGLNPLGIFRGFVSAGCEPDEMGIGPVFAVPRLLKRHGLTVGDIDLWELNEA
FAVQVIYCRDRLGIDPEKLNVDGGAIAVGHPYGMSGARLAGHALIEGRRRKAKYAVVTMCVGGGMGAAGLFEVLQ
>Mature_394_residues
TEAVIVSTARTPIGKAYRGALNATEGATLLGHAIGEAAVRAGVDPQEIEDVVMGVAMQQGATGGNIARKALLRAGLPVTV
AGATIDRQCASGLQAIALAARSVLFDGVGIAVGGGGESISLVQNEHANTFHAEDPALLEIKREVYMPMIDTAEVVAKRYG
ISRQAQDEYALESQRRTAAAQQEGRFKDELAPISTRMAIVDKETGNVTFRDVTLSQDEGPRPDTSAEGLAGLKPVRGEGC
TITAGNASQLSDGASAAVIMSGTEASRRGLNPLGIFRGFVSAGCEPDEMGIGPVFAVPRLLKRHGLTVGDIDLWELNEAF
AVQVIYCRDRLGIDPEKLNVDGGAIAVGHPYGMSGARLAGHALIEGRRRKAKYAVVTMCVGGGMGAAGLFEVLQ

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4501853, Length=393, Percent_Identity=41.9847328244275, Blast_Score=279, Evalue=3e-75,
Organism=Homo sapiens, GI167614485, Length=398, Percent_Identity=39.4472361809045, Blast_Score=245, Evalue=6e-65,
Organism=Homo sapiens, GI4557237, Length=407, Percent_Identity=37.1007371007371, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI148539872, Length=410, Percent_Identity=36.5853658536585, Blast_Score=221, Evalue=8e-58,
Organism=Homo sapiens, GI4504327, Length=441, Percent_Identity=29.9319727891156, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI194353979, Length=247, Percent_Identity=34.412955465587, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1787663, Length=412, Percent_Identity=41.747572815534, Blast_Score=251, Evalue=6e-68,
Organism=Escherichia coli, GI1788554, Length=408, Percent_Identity=41.1764705882353, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI87082165, Length=403, Percent_Identity=39.2059553349876, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI48994986, Length=404, Percent_Identity=37.6237623762376, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1788683, Length=419, Percent_Identity=30.7875894988067, Blast_Score=166, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI133906874, Length=399, Percent_Identity=37.34335839599, Blast_Score=224, Evalue=6e-59,
Organism=Caenorhabditis elegans, GI17535921, Length=414, Percent_Identity=37.1980676328502, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI25147385, Length=400, Percent_Identity=34.25, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17551802, Length=445, Percent_Identity=28.7640449438202, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17535917, Length=390, Percent_Identity=28.4615384615385, Blast_Score=128, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6322031, Length=401, Percent_Identity=36.9077306733167, Blast_Score=233, Evalue=5e-62,
Organism=Saccharomyces cerevisiae, GI6325229, Length=410, Percent_Identity=31.219512195122, Blast_Score=174, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24655093, Length=409, Percent_Identity=38.3863080684597, Blast_Score=252, Evalue=4e-67,
Organism=Drosophila melanogaster, GI17648125, Length=399, Percent_Identity=37.593984962406, Blast_Score=230, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24640423, Length=403, Percent_Identity=35.9801488833747, Blast_Score=225, Evalue=5e-59,
Organism=Drosophila melanogaster, GI17137578, Length=439, Percent_Identity=30.9794988610478, Blast_Score=173, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 41190; Mature: 41059

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEAVIVSTARTPIGKAYRGALNATEGATLLGHAIGEAAVRAGVDPQEIEDVVMGVAMQQ
CCCEEEEECCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
GATGGNIARKALLRAGLPVTVAGATIDRQCASGLQAIALAARSVLFDGVGIAVGGGGESI
CCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEE
SLVQNEHANTFHAEDPALLEIKREVYMPMIDTAEVVAKRYGISRQAQDEYALESQRRTAA
EEEECCCCCEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
AQQEGRFKDELAPISTRMAIVDKETGNVTFRDVTLSQDEGPRPDTSAEGLAGLKPVRGEG
HHHCCCCHHHHCCCCEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
CTITAGNASQLSDGASAAVIMSGTEASRRGLNPLGIFRGFVSAGCEPDEMGIGPVFAVPR
CEEECCCHHHCCCCCCEEEEEECCHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHH
LLKRHGLTVGDIDLWELNEAFAVQVIYCRDRLGIDPEKLNVDGGAIAVGHPYGMSGARLA
HHHHCCCEECCEEHEECCCCCEEEEEEEECCCCCCHHHEECCCCEEEECCCCCCCCCHHH
GHALIEGRRRKAKYAVVTMCVGGGMGAAGLFEVLQ
HHHHHHCHHHCCEEEEEEEEECCCCCHHHHHHHHC
>Mature Secondary Structure 
TEAVIVSTARTPIGKAYRGALNATEGATLLGHAIGEAAVRAGVDPQEIEDVVMGVAMQQ
CCEEEEECCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
GATGGNIARKALLRAGLPVTVAGATIDRQCASGLQAIALAARSVLFDGVGIAVGGGGESI
CCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEE
SLVQNEHANTFHAEDPALLEIKREVYMPMIDTAEVVAKRYGISRQAQDEYALESQRRTAA
EEEECCCCCEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
AQQEGRFKDELAPISTRMAIVDKETGNVTFRDVTLSQDEGPRPDTSAEGLAGLKPVRGEG
HHHCCCCHHHHCCCCEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
CTITAGNASQLSDGASAAVIMSGTEASRRGLNPLGIFRGFVSAGCEPDEMGIGPVFAVPR
CEEECCCHHHCCCCCCEEEEEECCHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHH
LLKRHGLTVGDIDLWELNEAFAVQVIYCRDRLGIDPEKLNVDGGAIAVGHPYGMSGARLA
HHHHCCCEECCEEHEECCCCCEEEEEEEECCCCCCHHHEECCCCEEEECCCCCCCCCHHH
GHALIEGRRRKAKYAVVTMCVGGGMGAAGLFEVLQ
HHHHHHCHHHCCEEEEEEEEECCCCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]