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The map label for this gene is glpK [H]

Identifier: 75674479

GI number: 75674479

Start: 315223

End: 316782

Strand: Direct

Name: glpK [H]

Synonym: Nwi_0281

Alternate gene names: 75674479

Gene position: 315223-316782 (Clockwise)

Preceding gene: 75674477

Following gene: 75674485

Centisome position: 9.27

GC content: 66.54

Gene sequence:

>1560_bases
TTGCCACTCTGGCGCGTTGCGCCGCCGCCGCGAGCCCGCAAGTTCGAGAACAACGTCATGGTCACCCACGTCCTCGCCAT
TGATCAAGGCACCACATCGTCACGGGCCATCGTGTTTCGCGCCGACCTCACCGTCGCCGCGATCGCGCAGCAGGAATTCC
CGCAGCACTTTCCGGCGTCGGGCTGGGTCGAACATGAGCCGGAAGACATCTGGACCTCGACCGTTGCAACCTGCCGGCAG
GCGATGAAGAAGGCGGGCGTCACGCCGAAGGACATCGCCGCCATCGGCATCACCAATCAGCGGGAAACCACGGTGGTCTG
GGACCGCGTCACCGGAAAGGCGGTGCGTCGCGCCATCGTGTGGCAGGACCGGCGCACTGCGGATGTCTGCGCGCGGCTGA
AAGCCGAGGGTCATGAGCCCGCGATATCGGCGAAAACCGGCTTGATCATCGACCCCTATTTTTCCGGCACCAAGGTCGCC
TGGATACTCGATTCGGTTCCGGGGGCGCGGGCGCGGGCGATGCGCGGTGAGCTGATGTTCGGGACCGTGGATTGTTATCT
CTTGTGGCGGCTGACCGGCGGCAAGGTGCACGCGACCGACGCCACCAATGCGTCGCGCACCATGCTGTTCGACATTCACA
CCGGGCACTGGGATGACGCGCTCCTGGATATTCTCCGGATACCGCGTGCGATGCTGCCGGAGGTGAAGGATTCCTCGGCG
CATTTCGGCGACAGCGCGCCTGAATTGTTCGGCGCGCCGATCGCGATCCGGGGCATCGCCGGCGATCAGCAGGCGGCGGT
GGTGGGTCAGGCGTGCTTCACGCCCGGAATGATCAAGTCGACCTACGGCACCGGCTGCTTCGCGCTGCTCAACACCGGCG
CGACGCCGGTCGCCTCGACCAACAAGCTGCTGACCACGATCGCCTATCAACTGAACGGCGAGCGTACCTACGCGCTGGAA
GGTTCGATCTTCGTCGCCGGCTCGGCGGTGCAGTGGCTGCGGGACGGCCTGGGCCTCATCAAGAATGCTTCGGAGACGGA
GCCGCTGGCGGAGCAGTCCGATCCCGTCCAATCGGTGTATCTTGTGCCGGCGTTCGTCGGCATGGGTGCGCCGTATTGGA
ACCCGAATGTGCGTGGCGCGCTGTTCGGACTGACCCGCAACACCGGGCCGGCGGAACTCGCTCACGCGGCGCTGGAAAGC
GTCTGCTACCAGACATTTGATCTTTGGGAGGCGATGCGCGCCGATTGGCCGGCCGACGCCGCCGCGCACACCGTGCTGGG
GGTCGACGGCGGCATGACGGCCTCCAACTGGACCATGCAGCGTCTCGCGGACCTGCTCGGGGTACCGGTCGACCGGCCGG
TCATTCAGGAGACCACGGCGCTGGGCGCGGCGTACCTCGCGGGGCTCTCCGCGGGCGTTTATCCGGAGCCGTCGCGGTTC
GCCGACAATTGGCGGCGTGACCGCTGTTTCAAGCCGGCCATGAGCGCCGAGACGCGTGCGCGGAAGCTCAAGGGATGGAC
AGCCGCGGTGCGTGGCGTGCTGGCGAGCGACGAGGGGTAA

Upstream 100 bases:

>100_bases
GGGCGGAGAAGTCGCGCCAGGCATCTTATCCTTATCGTCCGTCGAAGCGTGAATCAAAACGAAGGCACAAACTTGACCTT
GGACAGGCAATCCGCTTGCC

Downstream 100 bases:

>100_bases
GGCGGGTATCCACGCGCGCCCGACAGGCGCGGCAAGGGAAGATCGCTCAAGTCCGTAAGGCGACCGCCGCCTTGCGGCGC
AGTTGTCGGATTTCCTCGAA

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MPLWRVAPPPRARKFENNVMVTHVLAIDQGTTSSRAIVFRADLTVAAIAQQEFPQHFPASGWVEHEPEDIWTSTVATCRQ
AMKKAGVTPKDIAAIGITNQRETTVVWDRVTGKAVRRAIVWQDRRTADVCARLKAEGHEPAISAKTGLIIDPYFSGTKVA
WILDSVPGARARAMRGELMFGTVDCYLLWRLTGGKVHATDATNASRTMLFDIHTGHWDDALLDILRIPRAMLPEVKDSSA
HFGDSAPELFGAPIAIRGIAGDQQAAVVGQACFTPGMIKSTYGTGCFALLNTGATPVASTNKLLTTIAYQLNGERTYALE
GSIFVAGSAVQWLRDGLGLIKNASETEPLAEQSDPVQSVYLVPAFVGMGAPYWNPNVRGALFGLTRNTGPAELAHAALES
VCYQTFDLWEAMRADWPADAAAHTVLGVDGGMTASNWTMQRLADLLGVPVDRPVIQETTALGAAYLAGLSAGVYPEPSRF
ADNWRRDRCFKPAMSAETRARKLKGWTAAVRGVLASDEG

Sequences:

>Translated_519_residues
MPLWRVAPPPRARKFENNVMVTHVLAIDQGTTSSRAIVFRADLTVAAIAQQEFPQHFPASGWVEHEPEDIWTSTVATCRQ
AMKKAGVTPKDIAAIGITNQRETTVVWDRVTGKAVRRAIVWQDRRTADVCARLKAEGHEPAISAKTGLIIDPYFSGTKVA
WILDSVPGARARAMRGELMFGTVDCYLLWRLTGGKVHATDATNASRTMLFDIHTGHWDDALLDILRIPRAMLPEVKDSSA
HFGDSAPELFGAPIAIRGIAGDQQAAVVGQACFTPGMIKSTYGTGCFALLNTGATPVASTNKLLTTIAYQLNGERTYALE
GSIFVAGSAVQWLRDGLGLIKNASETEPLAEQSDPVQSVYLVPAFVGMGAPYWNPNVRGALFGLTRNTGPAELAHAALES
VCYQTFDLWEAMRADWPADAAAHTVLGVDGGMTASNWTMQRLADLLGVPVDRPVIQETTALGAAYLAGLSAGVYPEPSRF
ADNWRRDRCFKPAMSAETRARKLKGWTAAVRGVLASDEG
>Mature_518_residues
PLWRVAPPPRARKFENNVMVTHVLAIDQGTTSSRAIVFRADLTVAAIAQQEFPQHFPASGWVEHEPEDIWTSTVATCRQA
MKKAGVTPKDIAAIGITNQRETTVVWDRVTGKAVRRAIVWQDRRTADVCARLKAEGHEPAISAKTGLIIDPYFSGTKVAW
ILDSVPGARARAMRGELMFGTVDCYLLWRLTGGKVHATDATNASRTMLFDIHTGHWDDALLDILRIPRAMLPEVKDSSAH
FGDSAPELFGAPIAIRGIAGDQQAAVVGQACFTPGMIKSTYGTGCFALLNTGATPVASTNKLLTTIAYQLNGERTYALEG
SIFVAGSAVQWLRDGLGLIKNASETEPLAEQSDPVQSVYLVPAFVGMGAPYWNPNVRGALFGLTRNTGPAELAHAALESV
CYQTFDLWEAMRADWPADAAAHTVLGVDGGMTASNWTMQRLADLLGVPVDRPVIQETTALGAAYLAGLSAGVYPEPSRFA
DNWRRDRCFKPAMSAETRARKLKGWTAAVRGVLASDEG

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI189409120, Length=446, Percent_Identity=45.067264573991, Blast_Score=371, Evalue=1e-103,
Organism=Homo sapiens, GI4504007, Length=446, Percent_Identity=45.067264573991, Blast_Score=371, Evalue=1e-103,
Organism=Homo sapiens, GI42794763, Length=452, Percent_Identity=44.2477876106195, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI41393575, Length=446, Percent_Identity=44.6188340807175, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI88196792, Length=504, Percent_Identity=30.1587301587302, Blast_Score=265, Evalue=1e-70,
Organism=Escherichia coli, GI1790361, Length=497, Percent_Identity=52.112676056338, Blast_Score=525, Evalue=1e-150,
Organism=Escherichia coli, GI1789987, Length=472, Percent_Identity=27.5423728813559, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1787789, Length=510, Percent_Identity=25.2941176470588, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1790005, Length=519, Percent_Identity=23.8921001926782, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17535599, Length=497, Percent_Identity=38.6317907444668, Blast_Score=328, Evalue=4e-90,
Organism=Caenorhabditis elegans, GI71986140, Length=478, Percent_Identity=24.2677824267782, Blast_Score=168, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI71986147, Length=260, Percent_Identity=27.3076923076923, Blast_Score=109, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321755, Length=485, Percent_Identity=35.8762886597938, Blast_Score=268, Evalue=1e-72,
Organism=Drosophila melanogaster, GI17864214, Length=500, Percent_Identity=42.4, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24654573, Length=444, Percent_Identity=42.7927927927928, Blast_Score=336, Evalue=3e-92,
Organism=Drosophila melanogaster, GI24655555, Length=523, Percent_Identity=37.6673040152964, Blast_Score=333, Evalue=2e-91,
Organism=Drosophila melanogaster, GI24655560, Length=523, Percent_Identity=37.6673040152964, Blast_Score=333, Evalue=2e-91,
Organism=Drosophila melanogaster, GI24655570, Length=523, Percent_Identity=37.6673040152964, Blast_Score=333, Evalue=2e-91,
Organism=Drosophila melanogaster, GI24655574, Length=523, Percent_Identity=37.6673040152964, Blast_Score=333, Evalue=2e-91,
Organism=Drosophila melanogaster, GI24655565, Length=523, Percent_Identity=37.6673040152964, Blast_Score=333, Evalue=2e-91,
Organism=Drosophila melanogaster, GI19922058, Length=525, Percent_Identity=31.8095238095238, Blast_Score=254, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24652871, Length=492, Percent_Identity=32.520325203252, Blast_Score=245, Evalue=4e-65,
Organism=Drosophila melanogaster, GI24656367, Length=509, Percent_Identity=28.2907662082515, Blast_Score=180, Evalue=3e-45,
Organism=Drosophila melanogaster, GI21357867, Length=521, Percent_Identity=27.447216890595, Blast_Score=175, Evalue=7e-44,
Organism=Drosophila melanogaster, GI24656372, Length=346, Percent_Identity=28.3236994219653, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI45552909, Length=293, Percent_Identity=26.962457337884, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 56103; Mature: 55972

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLWRVAPPPRARKFENNVMVTHVLAIDQGTTSSRAIVFRADLTVAAIAQQEFPQHFPAS
CCCCCCCCCCCCHHCCCCEEEEEEEEECCCCCCCCEEEEEECCHHHHHHHHHHHCCCCCC
GWVEHEPEDIWTSTVATCRQAMKKAGVTPKDIAAIGITNQRETTVVWDRVTGKAVRRAIV
CCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCEEEEEECHHHHHHHHHHH
WQDRRTADVCARLKAEGHEPAISAKTGLIIDPYFSGTKVAWILDSVPGARARAMRGELMF
HCCCCHHHHHHHHHCCCCCCCEECCCCEEECCCCCCCEEEEEEECCCCHHHHHHCCCEEE
GTVDCYLLWRLTGGKVHATDATNASRTMLFDIHTGHWDDALLDILRIPRAMLPEVKDSSA
EEEEEEEEEEECCCEEEECCCCCCCCEEEEEEECCCCHHHHHHHHHCCHHHCCCCCCCCC
HFGDSAPELFGAPIAIRGIAGDQQAAVVGQACFTPGMIKSTYGTGCFALLNTGATPVAST
CCCCCCHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCHHHCCCCCEEEECCCCCCCCCCC
NKLLTTIAYQLNGERTYALEGSIFVAGSAVQWLRDGLGLIKNASETEPLAEQSDPVQSVY
CHHEEEEEEEECCCEEEEECCCEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEE
LVPAFVGMGAPYWNPNVRGALFGLTRNTGPAELAHAALESVCYQTFDLWEAMRADWPADA
HHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
AAHTVLGVDGGMTASNWTMQRLADLLGVPVDRPVIQETTALGAAYLAGLSAGVYPEPSRF
HHHEEEECCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHH
ADNWRRDRCFKPAMSAETRARKLKGWTAAVRGVLASDEG
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PLWRVAPPPRARKFENNVMVTHVLAIDQGTTSSRAIVFRADLTVAAIAQQEFPQHFPAS
CCCCCCCCCCCHHCCCCEEEEEEEEECCCCCCCCEEEEEECCHHHHHHHHHHHCCCCCC
GWVEHEPEDIWTSTVATCRQAMKKAGVTPKDIAAIGITNQRETTVVWDRVTGKAVRRAIV
CCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCEEEEEECHHHHHHHHHHH
WQDRRTADVCARLKAEGHEPAISAKTGLIIDPYFSGTKVAWILDSVPGARARAMRGELMF
HCCCCHHHHHHHHHCCCCCCCEECCCCEEECCCCCCCEEEEEEECCCCHHHHHHCCCEEE
GTVDCYLLWRLTGGKVHATDATNASRTMLFDIHTGHWDDALLDILRIPRAMLPEVKDSSA
EEEEEEEEEEECCCEEEECCCCCCCCEEEEEEECCCCHHHHHHHHHCCHHHCCCCCCCCC
HFGDSAPELFGAPIAIRGIAGDQQAAVVGQACFTPGMIKSTYGTGCFALLNTGATPVAST
CCCCCCHHHHCCCEEEEECCCCCHHHHHHHHHCCCCCHHHCCCCCEEEECCCCCCCCCCC
NKLLTTIAYQLNGERTYALEGSIFVAGSAVQWLRDGLGLIKNASETEPLAEQSDPVQSVY
CHHEEEEEEEECCCEEEEECCCEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEE
LVPAFVGMGAPYWNPNVRGALFGLTRNTGPAELAHAALESVCYQTFDLWEAMRADWPADA
HHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
AAHTVLGVDGGMTASNWTMQRLADLLGVPVDRPVIQETTALGAAYLAGLSAGVYPEPSRF
HHHEEEECCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHH
ADNWRRDRCFKPAMSAETRARKLKGWTAAVRGVLASDEG
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA