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The map label for this gene is yfhA [H]

Identifier: 74318789

GI number: 74318789

Start: 2853286

End: 2854635

Strand: Direct

Name: yfhA [H]

Synonym: Tbd_2771

Alternate gene names: 74318789

Gene position: 2853286-2854635 (Clockwise)

Preceding gene: 74318788

Following gene: 74318790

Centisome position: 98.06

GC content: 69.93

Gene sequence:

>1350_bases
ATGAAGAAGCTGACCATCCTCGTCGTCGACGATGACGCCGCGCTGCGCGAGCTGATCACGCTGCGGCTCGAAGCGAACGG
GTTTCGCGTGGTCGCCGTCGACAGCGGCGAGGCCGCGCTCGCGCAGCTCGCGCTGGCGCGGCCGGACGCCGTGCTGACCG
ACCTGCAGATGGCCGGCATGGACGGCATGGCGCTGTTCCGCGCGATACAGGCGCGCGATCCTGCGCTGCCCGTCGTCGTG
CTGACCGCGCACGGAACCATTCCCGACGCGGTCGCGGCGACCCAGCAAGGGCTGTTCGGCTATCTGACCAAGCCCTACGA
CGCGCCGACGCTGATCGAACTCCTGAAGCGCGCGACCCGCCTGGGCGTAAGCGCCGCGGAGGTCGCGGACGACTGGCGCG
CCGAAATCATCACCGCCAGCCCGGCGATGGAAGCCTTGCTGGCCGAGGCGCGGCTCGCCGCGCAGAGCGAAGCCTCGCTG
CTGATCCAGGGCGAATCCGGCACCGGCAAGGAGTTGCTCGCGCGGGCCATCCACCGCGCCAGCCCGCGCCGCGCCAAGCC
ATTCGTCGCGATCAACTGCGGCGCGATCCCGGCCGAACTGCTCGAATCCGAGCTCTTCGGCCACCTCAAGGGCGCCTTCA
CGGGCGCGGCGCGCGACAATCCGGGCCTCTTCATGAGCGCGCAGGGCGGCACGGTGTTTCTCGACGAGATCGGCGACATG
CCGCTGCCGCTGCAGGTCAAGCTCCTGCGCGTCCTGCAGGAAGGCGAGGTGCGTCCGGTGGGGGCGACCGAGACGCGCCC
GGTCGACGTGCGCATCCTCTCGGCGACACACAGGAACCTCGAGGATGCCATCGGCAGCGGCGAATTCCGCGAGGACCTCT
ACTACCGTCTCAACGTCGTCAACCTCATGCTGCCGCCGCTGCGCGAGCGGCGCGAGGACATTCCGCTGCTGGCGCAGCAT
TTCCTCGCCGAGCTGACCGAGAAATACGACCGTCGTGTACGCGGCTTCGCCCCCGACGCGCTCGACATGCTGGCCGCCGC
CGATTGGCCGGGCAACATCCGCCAGCTGCGCAACGTGCTCGAACAGTGCGTCGCCCTCTGTACGACGCCGACGATCCCCG
GCAGCCTCGTCGCGCGCGCACTGCGCGAGAAACCGGCGGAGATTCAGCCGCTCGCCGAAGCCCGCGCCGCCTTCGAGCGC
GACTATCTCATCTCCCTGCTCAAGCTCACGCGCGGCCAGGTCAGCGAAGTCGCGCGGCTCGCCGGACGCAACCGCACCGA
GGTCTACCGGCTGCTGCAGCGTCACGGCCTGACGCCCGCGCTGTTCAAGGATCGCGACGACGCGCCCTGA

Upstream 100 bases:

>100_bases
AGCAGCAGAACGCACGCGTCCACGAACTGCAGGAGAAACTCGAGCAGATCAAGGCGCTCGAGAAATCGCTGCAGCAGCGC
GGCGTCGTGCCGAAAGCGCC

Downstream 100 bases:

>100_bases
GCGTCTTCGCCGGGCGACAGCGCCGGGCGGCCTTAGCGCTAACGGCTCGACCCGTCACGGCACAATGGATTGATTCTCAA
GGGGAAAACGGCCCTGGCAC

Product: two component Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MKKLTILVVDDDAALRELITLRLEANGFRVVAVDSGEAALAQLALARPDAVLTDLQMAGMDGMALFRAIQARDPALPVVV
LTAHGTIPDAVAATQQGLFGYLTKPYDAPTLIELLKRATRLGVSAAEVADDWRAEIITASPAMEALLAEARLAAQSEASL
LIQGESGTGKELLARAIHRASPRRAKPFVAINCGAIPAELLESELFGHLKGAFTGAARDNPGLFMSAQGGTVFLDEIGDM
PLPLQVKLLRVLQEGEVRPVGATETRPVDVRILSATHRNLEDAIGSGEFREDLYYRLNVVNLMLPPLRERREDIPLLAQH
FLAELTEKYDRRVRGFAPDALDMLAAADWPGNIRQLRNVLEQCVALCTTPTIPGSLVARALREKPAEIQPLAEARAAFER
DYLISLLKLTRGQVSEVARLAGRNRTEVYRLLQRHGLTPALFKDRDDAP

Sequences:

>Translated_449_residues
MKKLTILVVDDDAALRELITLRLEANGFRVVAVDSGEAALAQLALARPDAVLTDLQMAGMDGMALFRAIQARDPALPVVV
LTAHGTIPDAVAATQQGLFGYLTKPYDAPTLIELLKRATRLGVSAAEVADDWRAEIITASPAMEALLAEARLAAQSEASL
LIQGESGTGKELLARAIHRASPRRAKPFVAINCGAIPAELLESELFGHLKGAFTGAARDNPGLFMSAQGGTVFLDEIGDM
PLPLQVKLLRVLQEGEVRPVGATETRPVDVRILSATHRNLEDAIGSGEFREDLYYRLNVVNLMLPPLRERREDIPLLAQH
FLAELTEKYDRRVRGFAPDALDMLAAADWPGNIRQLRNVLEQCVALCTTPTIPGSLVARALREKPAEIQPLAEARAAFER
DYLISLLKLTRGQVSEVARLAGRNRTEVYRLLQRHGLTPALFKDRDDAP
>Mature_449_residues
MKKLTILVVDDDAALRELITLRLEANGFRVVAVDSGEAALAQLALARPDAVLTDLQMAGMDGMALFRAIQARDPALPVVV
LTAHGTIPDAVAATQQGLFGYLTKPYDAPTLIELLKRATRLGVSAAEVADDWRAEIITASPAMEALLAEARLAAQSEASL
LIQGESGTGKELLARAIHRASPRRAKPFVAINCGAIPAELLESELFGHLKGAFTGAARDNPGLFMSAQGGTVFLDEIGDM
PLPLQVKLLRVLQEGEVRPVGATETRPVDVRILSATHRNLEDAIGSGEFREDLYYRLNVVNLMLPPLRERREDIPLLAQH
FLAELTEKYDRRVRGFAPDALDMLAAADWPGNIRQLRNVLEQCVALCTTPTIPGSLVARALREKPAEIQPLAEARAAFER
DYLISLLKLTRGQVSEVARLAGRNRTEVYRLLQRHGLTPALFKDRDDAP

Specific function: Probable member of a two-component regulatory system yfhA/yfhK [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788905, Length=443, Percent_Identity=55.7562076749436, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI1790437, Length=446, Percent_Identity=44.8430493273543, Blast_Score=351, Evalue=5e-98,
Organism=Escherichia coli, GI1790299, Length=472, Percent_Identity=41.1016949152542, Blast_Score=342, Evalue=2e-95,
Organism=Escherichia coli, GI1788550, Length=453, Percent_Identity=40.8388520971302, Blast_Score=339, Evalue=2e-94,
Organism=Escherichia coli, GI87082117, Length=321, Percent_Identity=47.0404984423676, Blast_Score=271, Evalue=7e-74,
Organism=Escherichia coli, GI1789233, Length=235, Percent_Identity=53.1914893617021, Blast_Score=249, Evalue=4e-67,
Organism=Escherichia coli, GI1789087, Length=315, Percent_Identity=44.1269841269841, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1786524, Length=253, Percent_Identity=49.0118577075099, Blast_Score=228, Evalue=6e-61,
Organism=Escherichia coli, GI87082152, Length=325, Percent_Identity=40.3076923076923, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1787583, Length=351, Percent_Identity=37.6068376068376, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI87081872, Length=268, Percent_Identity=40.6716417910448, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI87081858, Length=319, Percent_Identity=32.2884012539185, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1789828, Length=272, Percent_Identity=33.8235294117647, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1786599, Length=119, Percent_Identity=35.2941176470588, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1788191, Length=119, Percent_Identity=36.9747899159664, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786911, Length=116, Percent_Identity=31.8965517241379, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 48926; Mature: 48926

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLTILVVDDDAALRELITLRLEANGFRVVAVDSGEAALAQLALARPDAVLTDLQMAGM
CCEEEEEEECCCHHHHHHHHHEEECCCEEEEEECCCHHHHHHHHHCCCCHHHHHHHHCCC
DGMALFRAIQARDPALPVVVLTAHGTIPDAVAATQQGLFGYLTKPYDAPTLIELLKRATR
CHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHH
LGVSAAEVADDWRAEIITASPAMEALLAEARLAAQSEASLLIQGESGTGKELLARAIHRA
HCCHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHC
SPRRAKPFVAINCGAIPAELLESELFGHLKGAFTGAARDNPGLFMSAQGGTVFLDEIGDM
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEEECCCCC
PLPLQVKLLRVLQEGEVRPVGATETRPVDVRILSATHRNLEDAIGSGEFREDLYYRLNVV
CCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECHHHCCHHHHHCCCHHHHHHHHHHHHH
NLMLPPLRERREDIPLLAQHFLAELTEKYDRRVRGFAPDALDMLAAADWPGNIRQLRNVL
HHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHH
EQCVALCTTPTIPGSLVARALREKPAEIQPLAEARAAFERDYLISLLKLTRGQVSEVARL
HHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGRNRTEVYRLLQRHGLTPALFKDRDDAP
HCCCHHHHHHHHHHCCCCHHHHCCCCCCC
>Mature Secondary Structure
MKKLTILVVDDDAALRELITLRLEANGFRVVAVDSGEAALAQLALARPDAVLTDLQMAGM
CCEEEEEEECCCHHHHHHHHHEEECCCEEEEEECCCHHHHHHHHHCCCCHHHHHHHHCCC
DGMALFRAIQARDPALPVVVLTAHGTIPDAVAATQQGLFGYLTKPYDAPTLIELLKRATR
CHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHH
LGVSAAEVADDWRAEIITASPAMEALLAEARLAAQSEASLLIQGESGTGKELLARAIHRA
HCCHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHC
SPRRAKPFVAINCGAIPAELLESELFGHLKGAFTGAARDNPGLFMSAQGGTVFLDEIGDM
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEEECCCCC
PLPLQVKLLRVLQEGEVRPVGATETRPVDVRILSATHRNLEDAIGSGEFREDLYYRLNVV
CCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECHHHCCHHHHHCCCHHHHHHHHHHHHH
NLMLPPLRERREDIPLLAQHFLAELTEKYDRRVRGFAPDALDMLAAADWPGNIRQLRNVL
HHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHH
EQCVALCTTPTIPGSLVARALREKPAEIQPLAEARAAFERDYLISLLKLTRGQVSEVARL
HHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGRNRTEVYRLLQRHGLTPALFKDRDDAP
HCCCHHHHHHHHHHCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]