Click here to switch to the map view.

The map label for this gene is prrB [H]

Identifier: 74318700

GI number: 74318700

Start: 2758249

End: 2759451

Strand: Direct

Name: prrB [H]

Synonym: Tbd_2682

Alternate gene names: 74318700

Gene position: 2758249-2759451 (Clockwise)

Preceding gene: 74318699

Following gene: 74318701

Centisome position: 94.79

GC content: 55.44

Gene sequence:

>1203_bases
GTGAATAGTCAATCGCTGAGTGCTCCCATAAGCTGGCGCCGGATGAAGCTGAAGTACCTGGTTGCATTGAAAAGTGGAGA
GGCCATTCCGGGAGAGTCAATCAAAGAGACTGGCGACTACCCAGTCTATGGAGGCAACGGTTTCCGTGGCTACACCAACA
GTTTTACGCACGAAGGCGAGCGCATCCTGATCGGTCGGCAAGGCGCTCTCTGTGGCAACATCAATTATGCGGAGGGAAAA
TTTTGGGCTACGGAGCACGCCATTGTTGCTACTCCGAAAACTAACTTCGAGACGGCCTGGCTCGGTGAAACCCTGCGGGT
CATGAACCTCAATCAGTACTCGCAGTCGGCTGCGCAACCGGGTATAGCTGTAGAAGTCGTTGAGAACCTTGTGATTGCTG
TTCCCCCAGAGGGGGAACAGCGCCGCATAGCAGATTCCTTGCACCAACTTTCAGCGCCGATTGACAAGCTGATCTTAGAA
AAACAGAAACTGCTTACCCTACTTACTGAAAAACGCCGTACCGTCATCGCCGACTTTCTGATCAAGGGACTCAACAAGGA
CACACCACGCCGTGACTCTGACATTCCATGGCTCGGCGAGATTCCGGCCCATTGGAAAGTCGAACGTGCCAAGTGGCTTT
TCACCGAGCGTGATGACCGCTCCGATTCTGGCGACGAGGAGTTGCTGACGGTGTCACACCTGACCGGCGTCACCAGTCGC
GCCGAGAAGGACGTGAACATGTTCATGGCCGAGAGCCTGGAAGGCTACAAGCGTTGCGAGGCGGGCGACTTGGTCATCAA
TACCTTGTGGGCATGGATGGGTGCGATGGGCATTGCACGCCAACCCGGCATCGTCAGCCCGGCCTACAACGTCTATCAGC
CTGTGGCACAACTTGACCCGGAATACATTGATCTGCTGGTGCGCACTCCACGCTTTGTGGAGGAAATCACTCGCTATTCC
AAAGGTGTTTGGTCATCGCGTCTGCGTCTATACCCTGAGGGACTCTACGAGGCATGGTTGCCTGTGCCTCCCCTTGATGA
GCAGCGGGATATCGTAGCCCGTGTGCAAGCCGAGACCAGGAAATTGGATGCCCTTGCGGAAGCCACCGAACGAACGGTTA
CCGTGCTCCAAGAACGACGTTCGGCATTGATCTCGGCCGCAGTGACCGGGCAACTTGATCTAGGGGACGCCCATGCAAAT
TAA

Upstream 100 bases:

>100_bases
ACCTATCTGCGGCCCTTGATGCGGGACGGGCGATTGGTGATGACCAATCCGGATGAGCCCAACGATCCGCAGCAGGCATA
TCGGGCAGCGGAGGTTCCCA

Downstream 100 bases:

>100_bases
CCGCCTTACGCTGACCAATTTTCGCGGGTTGACGAATTCCGTCTTGGACTTTGCGCCCGGGTTCAATCTGGTCGTCGGTG
TAAACGGCGTCGGCAAGACG

Product: putative restriction endonuclease S subunit

Products: NA

Alternate protein names: S.EcoprrI; Type I restriction enzyme EcoprrI specificity protein; S protein [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MNSQSLSAPISWRRMKLKYLVALKSGEAIPGESIKETGDYPVYGGNGFRGYTNSFTHEGERILIGRQGALCGNINYAEGK
FWATEHAIVATPKTNFETAWLGETLRVMNLNQYSQSAAQPGIAVEVVENLVIAVPPEGEQRRIADSLHQLSAPIDKLILE
KQKLLTLLTEKRRTVIADFLIKGLNKDTPRRDSDIPWLGEIPAHWKVERAKWLFTERDDRSDSGDEELLTVSHLTGVTSR
AEKDVNMFMAESLEGYKRCEAGDLVINTLWAWMGAMGIARQPGIVSPAYNVYQPVAQLDPEYIDLLVRTPRFVEEITRYS
KGVWSSRLRLYPEGLYEAWLPVPPLDEQRDIVARVQAETRKLDALAEATERTVTVLQERRSALISAAVTGQLDLGDAHAN

Sequences:

>Translated_400_residues
MNSQSLSAPISWRRMKLKYLVALKSGEAIPGESIKETGDYPVYGGNGFRGYTNSFTHEGERILIGRQGALCGNINYAEGK
FWATEHAIVATPKTNFETAWLGETLRVMNLNQYSQSAAQPGIAVEVVENLVIAVPPEGEQRRIADSLHQLSAPIDKLILE
KQKLLTLLTEKRRTVIADFLIKGLNKDTPRRDSDIPWLGEIPAHWKVERAKWLFTERDDRSDSGDEELLTVSHLTGVTSR
AEKDVNMFMAESLEGYKRCEAGDLVINTLWAWMGAMGIARQPGIVSPAYNVYQPVAQLDPEYIDLLVRTPRFVEEITRYS
KGVWSSRLRLYPEGLYEAWLPVPPLDEQRDIVARVQAETRKLDALAEATERTVTVLQERRSALISAAVTGQLDLGDAHAN
>Mature_400_residues
MNSQSLSAPISWRRMKLKYLVALKSGEAIPGESIKETGDYPVYGGNGFRGYTNSFTHEGERILIGRQGALCGNINYAEGK
FWATEHAIVATPKTNFETAWLGETLRVMNLNQYSQSAAQPGIAVEVVENLVIAVPPEGEQRRIADSLHQLSAPIDKLILE
KQKLLTLLTEKRRTVIADFLIKGLNKDTPRRDSDIPWLGEIPAHWKVERAKWLFTERDDRSDSGDEELLTVSHLTGVTSR
AEKDVNMFMAESLEGYKRCEAGDLVINTLWAWMGAMGIARQPGIVSPAYNVYQPVAQLDPEYIDLLVRTPRFVEEITRYS
KGVWSSRLRLYPEGLYEAWLPVPPLDEQRDIVARVQAETRKLDALAEATERTVTVLQERRSALISAAVTGQLDLGDAHAN

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 44805; Mature: 44805

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSQSLSAPISWRRMKLKYLVALKSGEAIPGESIKETGDYPVYGGNGFRGYTNSFTHEGE
CCCCCCCCCCHHHHHEEEEEEEECCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCC
RILIGRQGALCGNINYAEGKFWATEHAIVATPKTNFETAWLGETLRVMNLNQYSQSAAQP
EEEEECCCCEECCCCCCCCCEEECCCEEEECCCCCCCHHHHCCHHHEEECCHHHHHHCCC
GIAVEVVENLVIAVPPEGEQRRIADSLHQLSAPIDKLILEKQKLLTLLTEKRRTVIADFL
CHHHHHHCCCEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKGLNKDTPRRDSDIPWLGEIPAHWKVERAKWLFTERDDRSDSGDEELLTVSHLTGVTSR
HHHCCCCCCCCCCCCCCCCCCCCCEEEHHHHEEEECCCCCCCCCCHHEEEHHHHHHHHHH
AEKDVNMFMAESLEGYKRCEAGDLVINTLWAWMGAMGIARQPGIVSPAYNVYQPVAQLDP
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCH
EYIDLLVRTPRFVEEITRYSKGVWSSRLRLYPEGLYEAWLPVPPLDEQRDIVARVQAETR
HHHHHHHCCHHHHHHHHHHHCCHHHHHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHH
KLDALAEATERTVTVLQERRSALISAAVTGQLDLGDAHAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCC
>Mature Secondary Structure
MNSQSLSAPISWRRMKLKYLVALKSGEAIPGESIKETGDYPVYGGNGFRGYTNSFTHEGE
CCCCCCCCCCHHHHHEEEEEEEECCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCC
RILIGRQGALCGNINYAEGKFWATEHAIVATPKTNFETAWLGETLRVMNLNQYSQSAAQP
EEEEECCCCEECCCCCCCCCEEECCCEEEECCCCCCCHHHHCCHHHEEECCHHHHHHCCC
GIAVEVVENLVIAVPPEGEQRRIADSLHQLSAPIDKLILEKQKLLTLLTEKRRTVIADFL
CHHHHHHCCCEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKGLNKDTPRRDSDIPWLGEIPAHWKVERAKWLFTERDDRSDSGDEELLTVSHLTGVTSR
HHHCCCCCCCCCCCCCCCCCCCCCEEEHHHHEEEECCCCCCCCCCHHEEEHHHHHHHHHH
AEKDVNMFMAESLEGYKRCEAGDLVINTLWAWMGAMGIARQPGIVSPAYNVYQPVAQLDP
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCH
EYIDLLVRTPRFVEEITRYSKGVWSSRLRLYPEGLYEAWLPVPPLDEQRDIVARVQAETR
HHHHHHHCCHHHHHHHHHHHCCHHHHHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHH
KLDALAEATERTVTVLQERRSALISAAVTGQLDLGDAHAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1691706 [H]