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The map label for this gene is mpl [H]

Identifier: 74318590

GI number: 74318590

Start: 2646358

End: 2647758

Strand: Direct

Name: mpl [H]

Synonym: Tbd_2572

Alternate gene names: 74318590

Gene position: 2646358-2647758 (Clockwise)

Preceding gene: 74318589

Following gene: 74318592

Centisome position: 90.95

GC content: 67.38

Gene sequence:

>1401_bases
ATGCACATCCACATCCTCGGCATCTGCGGCACCTTCATGGGTGGAATCGCCGCGATCGCGCGCAACGCCGGCCACACCGT
CACCGGCTGCGACGCCAACGTCTATCCGCCGATGTCCGACCAGCTGCGCGCGCTCGGCATCGAACTGACCGAAGGCTGGG
ACGCCGGACAGGTCCGACTCGAGCCCGACGCCTTCGTCGTCGGCAACGTCGTCACGCGCGGCAATCCCTTGATGGAAGCC
ATACTCGAGCGCGGCCTGCCCTACACCTCTGGCCCGCAGTGGCTCGCCGACAACGTGCTGCGCGACAAGTGGGTGCTCGC
CGTCGCCGGCACCCACGGCAAGACGACCACCTCGGCGATGCTGGCCTGGATCCTCGAGGACGCGCGCCTCCATCCGGGCT
TCCTCGTCGGCGGCATCCCGCAGAATTTCGGCCTGTCGGCGCGGCTCACCGACAGCCCCTTCTTCGTCATCGAGGCCGAC
GAATACGACACCGCCTTCTTCGACAAACGCTCCAAGTTCGTCCACTACCGCCCGCGCACGGCGATCCTCAACAACCTCGA
GTTCGATCACGCCGACATCTTCCCCGACATCCAGGCGATCGAAACCCAGTTTCACCACCTCGTGCGCACGATTCCCGGCA
ACGGCCTCATCGTCGCCAACGGCCGCGAAGCCGCGCTCGATCGCGTGCTCGAGCGCGGCCTGTGGACGCCGGTCGAACGC
TTCGGCAGCATCGGCGGCTGGACCGCAGGCGAGGCCGATGCGAGCGGCAGCTTCGACGTCCACTTCAACAGCGCGCTGCA
AGGCCGCGTCCGCTGGGAGCTGATCGGCGAGCACAACCGCATGAACGCGCTCGCGGCCATCGCCGCCGCGCGTCACGCCG
GCGTGCCGGCGGCCGCCGCCATCGATGCACTCAGCCGCTTCGAGAACGTCAAGCGGCGCATGGAAATCCGCGGTGCCGTA
CACGGGATCACGGTCTACGACGACTTCGCCCACCATCCCACCGCGATCACCACTACGGTCCAGGGCCTGCGCGCCAAGGT
CGGCAACGCGCGCATCCTCGCCGTGCTCGAACCGCGCTCCAACACCATGAAGCTTGGCGTCATGAAGGACGCGCTCCCCG
CCAGCCTCGCCGGTGCGGACGAGGTGTACTGCTACGGCGCCAACCTCGGCTGGGATGCCGCCGAGGCGCTCGCGCCGCTC
GGCGAGAAGGCCCGCGTCTTCGACAACCTCGACAAGCTCGTCGCGCAACTCGTCGCTGACGGACGGCCCGGCGACCATGT
GCTGATGATGAGTAACGGGGGCTTCGGCGGCATTCACGCGAAGCTCCTGGATGCGCTGCATCATCACTATCACGAGGAGA
TCGTGCTGACGCCGATGCATCGGTATGGGGGGTATGCGTGA

Upstream 100 bases:

>100_bases
TGCTCACGCATCTGATCCGGCAGTCACGCGCCGCCGCCGTGGGATAATCCGCGCGCCCTAGCCCCTAGCCCCTAGCCCCT
AGCCCCTAGCCCCCAAGATC

Downstream 100 bases:

>100_bases
GGGAAGGCTGTCACCGCTAGGCGCGTCGGGGCGGAGCAACCGCCAGGGATTAGGGGCGAAGCAGGGCCGAGTAGTTAGCG
CTTTCGCAGGACTGTTGACC

Product: UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MHIHILGICGTFMGGIAAIARNAGHTVTGCDANVYPPMSDQLRALGIELTEGWDAGQVRLEPDAFVVGNVVTRGNPLMEA
ILERGLPYTSGPQWLADNVLRDKWVLAVAGTHGKTTTSAMLAWILEDARLHPGFLVGGIPQNFGLSARLTDSPFFVIEAD
EYDTAFFDKRSKFVHYRPRTAILNNLEFDHADIFPDIQAIETQFHHLVRTIPGNGLIVANGREAALDRVLERGLWTPVER
FGSIGGWTAGEADASGSFDVHFNSALQGRVRWELIGEHNRMNALAAIAAARHAGVPAAAAIDALSRFENVKRRMEIRGAV
HGITVYDDFAHHPTAITTTVQGLRAKVGNARILAVLEPRSNTMKLGVMKDALPASLAGADEVYCYGANLGWDAAEALAPL
GEKARVFDNLDKLVAQLVADGRPGDHVLMMSNGGFGGIHAKLLDALHHHYHEEIVLTPMHRYGGYA

Sequences:

>Translated_466_residues
MHIHILGICGTFMGGIAAIARNAGHTVTGCDANVYPPMSDQLRALGIELTEGWDAGQVRLEPDAFVVGNVVTRGNPLMEA
ILERGLPYTSGPQWLADNVLRDKWVLAVAGTHGKTTTSAMLAWILEDARLHPGFLVGGIPQNFGLSARLTDSPFFVIEAD
EYDTAFFDKRSKFVHYRPRTAILNNLEFDHADIFPDIQAIETQFHHLVRTIPGNGLIVANGREAALDRVLERGLWTPVER
FGSIGGWTAGEADASGSFDVHFNSALQGRVRWELIGEHNRMNALAAIAAARHAGVPAAAAIDALSRFENVKRRMEIRGAV
HGITVYDDFAHHPTAITTTVQGLRAKVGNARILAVLEPRSNTMKLGVMKDALPASLAGADEVYCYGANLGWDAAEALAPL
GEKARVFDNLDKLVAQLVADGRPGDHVLMMSNGGFGGIHAKLLDALHHHYHEEIVLTPMHRYGGYA
>Mature_466_residues
MHIHILGICGTFMGGIAAIARNAGHTVTGCDANVYPPMSDQLRALGIELTEGWDAGQVRLEPDAFVVGNVVTRGNPLMEA
ILERGLPYTSGPQWLADNVLRDKWVLAVAGTHGKTTTSAMLAWILEDARLHPGFLVGGIPQNFGLSARLTDSPFFVIEAD
EYDTAFFDKRSKFVHYRPRTAILNNLEFDHADIFPDIQAIETQFHHLVRTIPGNGLIVANGREAALDRVLERGLWTPVER
FGSIGGWTAGEADASGSFDVHFNSALQGRVRWELIGEHNRMNALAAIAAARHAGVPAAAAIDALSRFENVKRRMEIRGAV
HGITVYDDFAHHPTAITTTVQGLRAKVGNARILAVLEPRSNTMKLGVMKDALPASLAGADEVYCYGANLGWDAAEALAPL
GEKARVFDNLDKLVAQLVADGRPGDHVLMMSNGGFGGIHAKLLDALHHHYHEEIVLTPMHRYGGYA

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=449, Percent_Identity=58.1291759465479, Blast_Score=524, Evalue=1e-150,
Organism=Escherichia coli, GI1786279, Length=390, Percent_Identity=30.5128205128205, Blast_Score=108, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 50543; Mature: 50543

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIHILGICGTFMGGIAAIARNAGHTVTGCDANVYPPMSDQLRALGIELTEGWDAGQVRL
CEEEEEEHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHCCEEEECCCCCCEEEE
EPDAFVVGNVVTRGNPLMEAILERGLPYTSGPQWLADNVLRDKWVLAVAGTHGKTTTSAM
CCCCEEEECEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCHHHHHH
LAWILEDARLHPGFLVGGIPQNFGLSARLTDSPFFVIEADEYDTAFFDKRSKFVHYRPRT
HHHHHHHCCCCCCEEECCCCCCCCCEEEECCCCEEEEECCCCCCHHHHCCCCEEEECCHH
AILNNLEFDHADIFPDIQAIETQFHHLVRTIPGNGLIVANGREAALDRVLERGLWTPVER
HHHCCCCCCCHHCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCCCHHHH
FGSIGGWTAGEADASGSFDVHFNSALQGRVRWELIGEHNRMNALAAIAAARHAGVPAAAA
HCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHH
IDALSRFENVKRRMEIRGAVHGITVYDDFAHHPTAITTTVQGLRAKVGNARILAVLEPRS
HHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCC
NTMKLGVMKDALPASLAGADEVYCYGANLGWDAAEALAPLGEKARVFDNLDKLVAQLVAD
CEEEEEEEHHCCCHHHCCCCEEEEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHC
GRPGDHVLMMSNGGFGGIHAKLLDALHHHYHEEIVLTPMHRYGGYA
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEHHHCCCCC
>Mature Secondary Structure
MHIHILGICGTFMGGIAAIARNAGHTVTGCDANVYPPMSDQLRALGIELTEGWDAGQVRL
CEEEEEEHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHCCEEEECCCCCCEEEE
EPDAFVVGNVVTRGNPLMEAILERGLPYTSGPQWLADNVLRDKWVLAVAGTHGKTTTSAM
CCCCEEEECEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCHHHHHH
LAWILEDARLHPGFLVGGIPQNFGLSARLTDSPFFVIEADEYDTAFFDKRSKFVHYRPRT
HHHHHHHCCCCCCEEECCCCCCCCCEEEECCCCEEEEECCCCCCHHHHCCCCEEEECCHH
AILNNLEFDHADIFPDIQAIETQFHHLVRTIPGNGLIVANGREAALDRVLERGLWTPVER
HHHCCCCCCCHHCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCCCHHHH
FGSIGGWTAGEADASGSFDVHFNSALQGRVRWELIGEHNRMNALAAIAAARHAGVPAAAA
HCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHH
IDALSRFENVKRRMEIRGAVHGITVYDDFAHHPTAITTTVQGLRAKVGNARILAVLEPRS
HHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCC
NTMKLGVMKDALPASLAGADEVYCYGANLGWDAAEALAPLGEKARVFDNLDKLVAQLVAD
CEEEEEEEHHCCCHHHCCCCEEEEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHC
GRPGDHVLMMSNGGFGGIHAKLLDALHHHYHEEIVLTPMHRYGGYA
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]