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The map label for this gene is gacS [H]

Identifier: 74318589

GI number: 74318589

Start: 2644400

End: 2646304

Strand: Direct

Name: gacS [H]

Synonym: Tbd_2571

Alternate gene names: 74318589

Gene position: 2644400-2646304 (Clockwise)

Preceding gene: 74318588

Following gene: 74318590

Centisome position: 90.88

GC content: 70.55

Gene sequence:

>1905_bases
GTGACCCGACTGGGCGCCCTGCTGCGTCGACTCTCGGTCTCGAGCATCCGCAGCCGGATGCTCGTGGTCGCGCTGCTGCC
CGCACTGCTCGCCGAATTCGGCATGGCCACGTATTTCACGACTCAGGCGCTGCGCACCGCCGGCCGCGATGTCGAAGCGC
GCGCCGCGGCGGCGGCCCGCCATCTGGCAGCAGCGCTGCCGCACGCGCTGGCCAACGGCGATCTCGCGCTGGCCCGCGAC
TTGCTCGAGAGCGAAGCGCGGAATAATGGCCTCGCATTTGCCCACCTGGTCGACGCTACCGGAAATGTCGTCGCCCGCGG
CGGCGACGCCGCCGACCCGGGCGACACCGAACATTACCGGCAACGCGTCGCGCTGCGCCCCCCGGCCGCCGACGGGCGGC
CGAATCTTCAGCCGGCCAGCGCGGCCGGCGCCGGTTCGCTCGCCTACGTCGAGGTCGGGGCGACGCTCGGGCAAAGCAGC
GCGCTCCGGCGCGACGTGCTGCTGCACGCGGCGCTGCTGATGGCCATCGCGCTCGCCCTCACCGGTGCCCTGGCCTGGCG
GCTGTCGAGCCGGCTGGCGAGCCAGCTGCGCCACGTCGGACAAGTCGTCGAGCGGCTGGCGTGCGACGACCTGACCGTCC
GCACGCGCCTGCCGCCCGACGGTGAGGTCGGCGCGCTCGCAGCGGGGATCAACAACATGGCCGAGGCCTTGCAGCGGAAC
CGCAGCGAACTCGAGAAGCGGGTGCGCGAGGCGACCGCGAGCCTGACGGCGAAAAAGATCATGGCGGAGCGCGCGAATCA
CGCGAAGTCGCGCTTCTTCGCCGCGGCGAGCCACGATCTGCGCCAGCCGCTGCACGCGCTGTCGCTGTTCGTCGCCGCGC
TGAAGGCGCGCAATGAACAGGCCGAGACGCAGACGCTCATCGACAACATCGAGGCATCGACGGCGGCGATGGAGTTGCTG
TTCAACGCGCTGCTCGACATTTCCCGGCTCGACGCCGGCGCCATCGAGGCGCACCCGGTGCACTTCCCGCTCTGCAAGAT
GCTCGTCGATCTGGAACAACAGTTCGGCGCCGTCGCCGCGGAAAAACAGCTGCGGCTGCGCTTCCGCCCCTGCGACGCCA
CGGTGTACAGCGATCCGCTGCTGGTCGAGCGCATCCTCGCCAACCTGATCGCGAACGCGATCCGCTACACCGACGACGGC
GGTGTGCTGGTCGGCTGTCGGCGCCGCGGCCGGACCCTGCGCATCAGCGTGGTCGACACCGGCCGCGGCATTCCGCCCGA
CCAGCAGGAGAGCGTCTTCCAGGAATTCGTCCAGTTGCACAATCCGGCGCGCGACCGCAGCAAAGGCCTTGGGCTCGGGC
TCGCCATCGTCTCCCGCCTCGGCCGCCTGCTGGGGCATCGCGTCGATCTTCGCTCCGTGCCCGCGCACGGCTCGGTATTC
AGCATCGACGTCCCGCTCGGCGACGCCGCGCTCGTCCGCGCGCATCCGCCGGCGACCGCCCCGGCGCAAACGCCGGCGGA
TGCGCTGGTCGTGCTCGTCGACGACGAGAGTGCGATCCTGCGCGGCATGGCGGAGTTGTTCGACAACTGGGGCATCGACC
TGGTCACCGCCCACAGCGCCGACGAGGCGGAACAGTGGCTGGCGACGGTCGAACGCGTGCCCGACGTTATCGTCTCCGAC
TACCGCCTGCCGGACGACGACGGGATCGCTGTCATCGCCCGCCTGCGGCAGAAATTCGCGCGCGAGATCCCGGCCATCGT
GGTCACCGGAGACACCGCGCCGGACACGATCCTGCGCATCAACAGCGCCGGCTTTCCGCTCCTTCACAAGCCGCTGCGCC
CGGCCAAACTGCGCGCACTGCTCACGCATCTGATCCGGCAGTCACGCGCCGCCGCCGTGGGATAA

Upstream 100 bases:

>100_bases
AAAGCACGGTCAAGGTGCACATCGCGGCGATTTTTCGTGCCTTGAGCGTCACCAACCGCACCGAAGCCGTGCTGGCGATC
CAGCGCCTCACGCAAGGCTC

Downstream 100 bases:

>100_bases
TCCGCGCGCCCTAGCCCCTAGCCCCTAGCCCCTAGCCCCTAGCCCCCAAGATCATGCACATCCACATCCTCGGCATCTGC
GGCACCTTCATGGGTGGAAT

Product: periplasmic sensor hybrid histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 634; Mature: 633

Protein sequence:

>634_residues
MTRLGALLRRLSVSSIRSRMLVVALLPALLAEFGMATYFTTQALRTAGRDVEARAAAAARHLAAALPHALANGDLALARD
LLESEARNNGLAFAHLVDATGNVVARGGDAADPGDTEHYRQRVALRPPAADGRPNLQPASAAGAGSLAYVEVGATLGQSS
ALRRDVLLHAALLMAIALALTGALAWRLSSRLASQLRHVGQVVERLACDDLTVRTRLPPDGEVGALAAGINNMAEALQRN
RSELEKRVREATASLTAKKIMAERANHAKSRFFAAASHDLRQPLHALSLFVAALKARNEQAETQTLIDNIEASTAAMELL
FNALLDISRLDAGAIEAHPVHFPLCKMLVDLEQQFGAVAAEKQLRLRFRPCDATVYSDPLLVERILANLIANAIRYTDDG
GVLVGCRRRGRTLRISVVDTGRGIPPDQQESVFQEFVQLHNPARDRSKGLGLGLAIVSRLGRLLGHRVDLRSVPAHGSVF
SIDVPLGDAALVRAHPPATAPAQTPADALVVLVDDESAILRGMAELFDNWGIDLVTAHSADEAEQWLATVERVPDVIVSD
YRLPDDDGIAVIARLRQKFAREIPAIVVTGDTAPDTILRINSAGFPLLHKPLRPAKLRALLTHLIRQSRAAAVG

Sequences:

>Translated_634_residues
MTRLGALLRRLSVSSIRSRMLVVALLPALLAEFGMATYFTTQALRTAGRDVEARAAAAARHLAAALPHALANGDLALARD
LLESEARNNGLAFAHLVDATGNVVARGGDAADPGDTEHYRQRVALRPPAADGRPNLQPASAAGAGSLAYVEVGATLGQSS
ALRRDVLLHAALLMAIALALTGALAWRLSSRLASQLRHVGQVVERLACDDLTVRTRLPPDGEVGALAAGINNMAEALQRN
RSELEKRVREATASLTAKKIMAERANHAKSRFFAAASHDLRQPLHALSLFVAALKARNEQAETQTLIDNIEASTAAMELL
FNALLDISRLDAGAIEAHPVHFPLCKMLVDLEQQFGAVAAEKQLRLRFRPCDATVYSDPLLVERILANLIANAIRYTDDG
GVLVGCRRRGRTLRISVVDTGRGIPPDQQESVFQEFVQLHNPARDRSKGLGLGLAIVSRLGRLLGHRVDLRSVPAHGSVF
SIDVPLGDAALVRAHPPATAPAQTPADALVVLVDDESAILRGMAELFDNWGIDLVTAHSADEAEQWLATVERVPDVIVSD
YRLPDDDGIAVIARLRQKFAREIPAIVVTGDTAPDTILRINSAGFPLLHKPLRPAKLRALLTHLIRQSRAAAVG
>Mature_633_residues
TRLGALLRRLSVSSIRSRMLVVALLPALLAEFGMATYFTTQALRTAGRDVEARAAAAARHLAAALPHALANGDLALARDL
LESEARNNGLAFAHLVDATGNVVARGGDAADPGDTEHYRQRVALRPPAADGRPNLQPASAAGAGSLAYVEVGATLGQSSA
LRRDVLLHAALLMAIALALTGALAWRLSSRLASQLRHVGQVVERLACDDLTVRTRLPPDGEVGALAAGINNMAEALQRNR
SELEKRVREATASLTAKKIMAERANHAKSRFFAAASHDLRQPLHALSLFVAALKARNEQAETQTLIDNIEASTAAMELLF
NALLDISRLDAGAIEAHPVHFPLCKMLVDLEQQFGAVAAEKQLRLRFRPCDATVYSDPLLVERILANLIANAIRYTDDGG
VLVGCRRRGRTLRISVVDTGRGIPPDQQESVFQEFVQLHNPARDRSKGLGLGLAIVSRLGRLLGHRVDLRSVPAHGSVFS
IDVPLGDAALVRAHPPATAPAQTPADALVVLVDDESAILRGMAELFDNWGIDLVTAHSADEAEQWLATVERVPDVIVSDY
RLPDDDGIAVIARLRQKFAREIPAIVVTGDTAPDTILRINSAGFPLLHKPLRPAKLRALLTHLIRQSRAAAVG

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI87081816, Length=511, Percent_Identity=29.9412915851272, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1789149, Length=528, Percent_Identity=25, Blast_Score=137, Evalue=3e-33,
Organism=Escherichia coli, GI48994928, Length=380, Percent_Identity=26.5789473684211, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI145693157, Length=231, Percent_Identity=29.4372294372294, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1788713, Length=409, Percent_Identity=24.4498777506112, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1788393, Length=284, Percent_Identity=26.056338028169, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI1790861, Length=321, Percent_Identity=26.4797507788162, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1790346, Length=277, Percent_Identity=30.6859205776173, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1787894, Length=224, Percent_Identity=32.1428571428571, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1786912, Length=243, Percent_Identity=26.7489711934156, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1786783, Length=222, Percent_Identity=27.4774774774775, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1786600, Length=231, Percent_Identity=28.1385281385281, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1789808, Length=197, Percent_Identity=29.9492385786802, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI1790436, Length=131, Percent_Identity=30.5343511450382, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI1788279, Length=421, Percent_Identity=22.8028503562945, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 68023; Mature: 67892

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: PS50885 HAMP ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRLGALLRRLSVSSIRSRMLVVALLPALLAEFGMATYFTTQALRTAGRDVEARAAAAAR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
HLAAALPHALANGDLALARDLLESEARNNGLAFAHLVDATGNVVARGGDAADPGDTEHYR
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCEEECCCCCCCCCCHHHHH
QRVALRPPAADGRPNLQPASAAGAGSLAYVEVGATLGQSSALRRDVLLHAALLMAIALAL
HHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHCCHHHHHHHHHHHHHHHHHHHHHH
TGALAWRLSSRLASQLRHVGQVVERLACDDLTVRTRLPPDGEVGALAAGINNMAEALQRN
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHH
RSELEKRVREATASLTAKKIMAERANHAKSRFFAAASHDLRQPLHALSLFVAALKARNEQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
AETQTLIDNIEASTAAMELLFNALLDISRLDAGAIEAHPVHFPLCKMLVDLEQQFGAVAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
EKQLRLRFRPCDATVYSDPLLVERILANLIANAIRYTDDGGVLVGCRRRGRTLRISVVDT
HHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCEEEEECCCCCEEEEEEEEC
GRGIPPDQQESVFQEFVQLHNPARDRSKGLGLGLAIVSRLGRLLGHRVDLRSVPAHGSVF
CCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEE
SIDVPLGDAALVRAHPPATAPAQTPADALVVLVDDESAILRGMAELFDNWGIDLVTAHSA
EEECCCCCCEEEECCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCH
DEAEQWLATVERVPDVIVSDYRLPDDDGIAVIARLRQKFAREIPAIVVTGDTAPDTILRI
HHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCEEEE
NSAGFPLLHKPLRPAKLRALLTHLIRQSRAAAVG
CCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TRLGALLRRLSVSSIRSRMLVVALLPALLAEFGMATYFTTQALRTAGRDVEARAAAAAR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
HLAAALPHALANGDLALARDLLESEARNNGLAFAHLVDATGNVVARGGDAADPGDTEHYR
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCEEECCCCCCCCCCHHHHH
QRVALRPPAADGRPNLQPASAAGAGSLAYVEVGATLGQSSALRRDVLLHAALLMAIALAL
HHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHCCHHHHHHHHHHHHHHHHHHHHHH
TGALAWRLSSRLASQLRHVGQVVERLACDDLTVRTRLPPDGEVGALAAGINNMAEALQRN
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHH
RSELEKRVREATASLTAKKIMAERANHAKSRFFAAASHDLRQPLHALSLFVAALKARNEQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
AETQTLIDNIEASTAAMELLFNALLDISRLDAGAIEAHPVHFPLCKMLVDLEQQFGAVAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
EKQLRLRFRPCDATVYSDPLLVERILANLIANAIRYTDDGGVLVGCRRRGRTLRISVVDT
HHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCEEEEECCCCCEEEEEEEEC
GRGIPPDQQESVFQEFVQLHNPARDRSKGLGLGLAIVSRLGRLLGHRVDLRSVPAHGSVF
CCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEE
SIDVPLGDAALVRAHPPATAPAQTPADALVVLVDDESAILRGMAELFDNWGIDLVTAHSA
EEECCCCCCEEEECCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCH
DEAEQWLATVERVPDVIVSDYRLPDDDGIAVIARLRQKFAREIPAIVVTGDTAPDTILRI
HHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCEEEE
NSAGFPLLHKPLRPAKLRALLTHLIRQSRAAAVG
CCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]