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The map label for this gene is htrB [H]

Identifier: 74318541

GI number: 74318541

Start: 2595054

End: 2595965

Strand: Direct

Name: htrB [H]

Synonym: Tbd_2523

Alternate gene names: 74318541

Gene position: 2595054-2595965 (Clockwise)

Preceding gene: 74318540

Following gene: 74318542

Centisome position: 89.18

GC content: 68.53

Gene sequence:

>912_bases
GTGAAGCAATCGCGCGCGCAGCTCCTTCATCCCTCGCTTTTCGGCGTCTGGCTCGGCGTCGGCCTGCTGTGGCTGCTGCA
CTGGCTGCCTCTGCCGCTGCTGGCCGCGCTGGGCAACGGTCTCGGCTGGCTGCTCGCGATGCGGCCGGGCAAACGCAAGC
GCGTCGTCGCGACCAATCTTGCGCTGTGTTTTCCCGACGTCGCCGAGGCGACGCGCCGGCGCTGGCTGCGCCAGACCTTC
CAGGCGTCGATGCGGGCGGCGCTCGAATACGGCGTGCTGTGGTGGGGATCGGAGGCGCGGCTGCGCCGCGTAGTGCGCAT
CGACAATCCCGAAGCCGCGCGCGGCGACGGCAAGCGTCCGGTGATCTGGCTCGCGCCGCATTTCGTCGGCCTCGACATGG
GCGGCGTGCGGCTGACGCTCGATCACGCGATGGCGTCGATATACGCGCCCGCCAAGGACCCGGTCAGTGACAGATTCATC
CGCCGCGGGCGCACGCGCTTTTCCGACATCGTGCTGATCGCGCGCCACGAGGGCTTGAAGTCGACGCTCAATGCGATCAA
GTCCGGGCGCCCGTTCTATTACCTGCCCGACCAGGACCACGGCCGTCACAACGCAGTGTTCGTCCCCTTCTTCGGCGTGC
CCGCGGCGACCGTCTCGGCCTTGCCGCGGCTCGCCAGGCTGACGAACGCACAAGTCGTCCCGGTCGTGACCCGGCAACTG
CCGTGGGGACGCGGCTACGCCGTGCATTTCTACCCGCCCTGGGACGACTATCCGAGCGGCGACCTCGACGTCGACGTCGC
GCGCATGAATGCCTTCATCGAAGCCCGCGTCCGCGAGATGCCGGCGCAGTACCTGTGGCTGCATCGCCGCTTCAAGACCC
GGCCCGCAGGCGAGCGAAGCGTCTATGAGTAA

Upstream 100 bases:

>100_bases
AGGGCGTCGAAGCCTTCGTGCGGCGCTTTCCGGCGCAGTACATGTGGAGCTACAACCGCTACAAGCGCCCCGGCGGGGCC
CCGCCTCCGGACGCAACGAC

Downstream 100 bases:

>100_bases
GACGCCGTTCGAGGTTTCGCGCCTGGCCGAGGCGGACGTCGACGCCGTGCAATCGCTGGCGCGCACGGTGTGGCAGGCGA
CCTATCCGGCCCTGATCTCG

Product: lipid A biosynthesis lauroyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MKQSRAQLLHPSLFGVWLGVGLLWLLHWLPLPLLAALGNGLGWLLAMRPGKRKRVVATNLALCFPDVAEATRRRWLRQTF
QASMRAALEYGVLWWGSEARLRRVVRIDNPEAARGDGKRPVIWLAPHFVGLDMGGVRLTLDHAMASIYAPAKDPVSDRFI
RRGRTRFSDIVLIARHEGLKSTLNAIKSGRPFYYLPDQDHGRHNAVFVPFFGVPAATVSALPRLARLTNAQVVPVVTRQL
PWGRGYAVHFYPPWDDYPSGDLDVDVARMNAFIEARVREMPAQYLWLHRRFKTRPAGERSVYE

Sequences:

>Translated_303_residues
MKQSRAQLLHPSLFGVWLGVGLLWLLHWLPLPLLAALGNGLGWLLAMRPGKRKRVVATNLALCFPDVAEATRRRWLRQTF
QASMRAALEYGVLWWGSEARLRRVVRIDNPEAARGDGKRPVIWLAPHFVGLDMGGVRLTLDHAMASIYAPAKDPVSDRFI
RRGRTRFSDIVLIARHEGLKSTLNAIKSGRPFYYLPDQDHGRHNAVFVPFFGVPAATVSALPRLARLTNAQVVPVVTRQL
PWGRGYAVHFYPPWDDYPSGDLDVDVARMNAFIEARVREMPAQYLWLHRRFKTRPAGERSVYE
>Mature_303_residues
MKQSRAQLLHPSLFGVWLGVGLLWLLHWLPLPLLAALGNGLGWLLAMRPGKRKRVVATNLALCFPDVAEATRRRWLRQTF
QASMRAALEYGVLWWGSEARLRRVVRIDNPEAARGDGKRPVIWLAPHFVGLDMGGVRLTLDHAMASIYAPAKDPVSDRFI
RRGRTRFSDIVLIARHEGLKSTLNAIKSGRPFYYLPDQDHGRHNAVFVPFFGVPAATVSALPRLARLTNAQVVPVVTRQL
PWGRGYAVHFYPPWDDYPSGDLDVDVARMNAFIEARVREMPAQYLWLHRRFKTRPAGERSVYE

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=298, Percent_Identity=36.5771812080537, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI87082094, Length=300, Percent_Identity=34.3333333333333, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1788161, Length=303, Percent_Identity=32.6732673267327, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 34429; Mature: 34429

Theoretical pI: Translated: 11.34; Mature: 11.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQSRAQLLHPSLFGVWLGVGLLWLLHWLPLPLLAALGNGLGWLLAMRPGKRKRVVATNL
CCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEHHH
ALCFPDVAEATRRRWLRQTFQASMRAALEYGVLWWGSEARLRRVVRIDNPEAARGDGKRP
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCHHHHHHHEEECCCCCCCCCCCCC
VIWLAPHFVGLDMGGVRLTLDHAMASIYAPAKDPVSDRFIRRGRTRFSDIVLIARHEGLK
EEEECCCEEECCCCCEEEEHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHEEEEEECCCHH
STLNAIKSGRPFYYLPDQDHGRHNAVFVPFFGVPAATVSALPRLARLTNAQVVPVVTRQL
HHHHHHHCCCCEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEHHHHCC
PWGRGYAVHFYPPWDDYPSGDLDVDVARMNAFIEARVREMPAQYLWLHRRFKTRPAGERS
CCCCCEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCC
VYE
CCC
>Mature Secondary Structure
MKQSRAQLLHPSLFGVWLGVGLLWLLHWLPLPLLAALGNGLGWLLAMRPGKRKRVVATNL
CCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEHHH
ALCFPDVAEATRRRWLRQTFQASMRAALEYGVLWWGSEARLRRVVRIDNPEAARGDGKRP
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCHHHHHHHEEECCCCCCCCCCCCC
VIWLAPHFVGLDMGGVRLTLDHAMASIYAPAKDPVSDRFIRRGRTRFSDIVLIARHEGLK
EEEECCCEEECCCCCEEEEHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHEEEEEECCCHH
STLNAIKSGRPFYYLPDQDHGRHNAVFVPFFGVPAATVSALPRLARLTNAQVVPVVTRQL
HHHHHHHCCCCEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEHHHHCC
PWGRGYAVHFYPPWDDYPSGDLDVDVARMNAFIEARVREMPAQYLWLHRRFKTRPAGERS
CCCCCEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCC
VYE
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]