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The map label for this gene is purD [H]

Identifier: 74318477

GI number: 74318477

Start: 2530099

End: 2531376

Strand: Direct

Name: purD [H]

Synonym: Tbd_2459

Alternate gene names: 74318477

Gene position: 2530099-2531376 (Clockwise)

Preceding gene: 74318476

Following gene: 74318478

Centisome position: 86.95

GC content: 69.95

Gene sequence:

>1278_bases
ATGAAAATCCTCGTCATCGGATCCGGCGGACGCGAACACGCACTCGCCTGGAAGCTCGCGCAATCGCCCAAAGTGTCGCG
CGTCTTCGTCGCGCCCGGGAACGGCGGGACGGCGCGTGAGGAAGGCGTCGTCAACGTGCCGATTTCAGCGACCGCCGAAC
TCCTCGACTTCGCGTCGCGCGAAGACATCGGACTGACCGTCGTCGGTCCCGAAGCGCCGCTCGCGGCGGGCGTCGTCGAT
GCCTTCCGCGCCGCCGGGCGGAAGATTTTCGGGCCGACCCAGGCCGCCGCCCAGCTCGAGTCGTCGAAGGATTTCGCCAA
GCAGTTCATGGCGCGCCATCGCATTCCGACCGCGGCATTCGCGACCTTTAGCGACGCGGCCGCGGCGCACGTCTATATCG
ACGCCCAGGGCGCGCCGATCGTCGTCAAGGCCGATGGCCTCGCCGCGGGTAAGGGGGTCGTCGTCGCAGCGACGGCCGAC
GAGGCGCACGCCGCGGTCGATGCCATGCTCGCCGGCAACAGCATGGGCGAAGCTGGCCATCGCGTCGTGATCGAGGAATG
CCTCGTCGGCGAGGAAGCGAGCTTCATCGTTCTGGTCGACGGCGAGCACGTGCTGCCGCTCGCGTCGAGCCAGGACCACA
AGCGCCTGCAGGACGGCGACCAGGGCCCCAACACGGGCGGCATGGGCGCCTACTCGCCCGCGCCCGTGGTCACGCCGCAG
CTCCATGCGCGCATCATGCGCGAGGTCATCGTGCCGACCGTCGAGGGCATGGCGGCCGACGGCATCCGCTACACGGGCTT
CCTCTACGCCGGCATCATGGTCGCGGCCGACGGCAGCCCGAAGGTGCTCGAATTCAACTGCCGCATGGGCGACCCGGAAA
CCCAGCCGATCATGATGCGCTTGAAGTCCGATCTCGTCGCATTGCTCGAGGCCGCCGTCACGGGTCGGCTCGATCAGGTC
GACGCCGAGTGGGACCGCCGCACCGCGCTCGCCGTCGTGCTCGCCGCAGCCGGCTATCCCGACAGCCCGCAGAAGGGTGC
GCCGCTCGCCGCCCTGCCCGACGCGGCCGAGGATCTGCGGATCTTCCACGCCGGCACCTCGGCGCAGGACGGCCGCACGG
TCGTGAGCGGCGGCCGCGTCCTTGCCGTGACCGCGCTCGGCGACAGCGTGCGCATGGCGCAGAAGCGCGCCTACGACGCG
GTCGCCGCGATCCACTTCGACGGCATGCAATTCCGTCGCGACATCGGCTGGCGCGCGCTCGACCGCAGCAAGAAGTGA

Upstream 100 bases:

>100_bases
CAGGTTTCAGGGATCAGGGAATGCGCCCGAGGCCGCCATTTTCCGAATCCACTCCACCTGACCTCTCCTTGACCCCTGAA
TCCCGACCCCTGACCCCTCT

Downstream 100 bases:

>100_bases
GTCCCCGGGCGTGAGCACCGCGGAAAGCCGGGAAGGGGCGCAGCCGGGTCTGCACGCCTACCTCAGGCGCGGCGGCGTGA
TCGCGTATCCGACCGAATCG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MKILVIGSGGREHALAWKLAQSPKVSRVFVAPGNGGTAREEGVVNVPISATAELLDFASREDIGLTVVGPEAPLAAGVVD
AFRAAGRKIFGPTQAAAQLESSKDFAKQFMARHRIPTAAFATFSDAAAAHVYIDAQGAPIVVKADGLAAGKGVVVAATAD
EAHAAVDAMLAGNSMGEAGHRVVIEECLVGEEASFIVLVDGEHVLPLASSQDHKRLQDGDQGPNTGGMGAYSPAPVVTPQ
LHARIMREVIVPTVEGMAADGIRYTGFLYAGIMVAADGSPKVLEFNCRMGDPETQPIMMRLKSDLVALLEAAVTGRLDQV
DAEWDRRTALAVVLAAAGYPDSPQKGAPLAALPDAAEDLRIFHAGTSAQDGRTVVSGGRVLAVTALGDSVRMAQKRAYDA
VAAIHFDGMQFRRDIGWRALDRSKK

Sequences:

>Translated_425_residues
MKILVIGSGGREHALAWKLAQSPKVSRVFVAPGNGGTAREEGVVNVPISATAELLDFASREDIGLTVVGPEAPLAAGVVD
AFRAAGRKIFGPTQAAAQLESSKDFAKQFMARHRIPTAAFATFSDAAAAHVYIDAQGAPIVVKADGLAAGKGVVVAATAD
EAHAAVDAMLAGNSMGEAGHRVVIEECLVGEEASFIVLVDGEHVLPLASSQDHKRLQDGDQGPNTGGMGAYSPAPVVTPQ
LHARIMREVIVPTVEGMAADGIRYTGFLYAGIMVAADGSPKVLEFNCRMGDPETQPIMMRLKSDLVALLEAAVTGRLDQV
DAEWDRRTALAVVLAAAGYPDSPQKGAPLAALPDAAEDLRIFHAGTSAQDGRTVVSGGRVLAVTALGDSVRMAQKRAYDA
VAAIHFDGMQFRRDIGWRALDRSKK
>Mature_425_residues
MKILVIGSGGREHALAWKLAQSPKVSRVFVAPGNGGTAREEGVVNVPISATAELLDFASREDIGLTVVGPEAPLAAGVVD
AFRAAGRKIFGPTQAAAQLESSKDFAKQFMARHRIPTAAFATFSDAAAAHVYIDAQGAPIVVKADGLAAGKGVVVAATAD
EAHAAVDAMLAGNSMGEAGHRVVIEECLVGEEASFIVLVDGEHVLPLASSQDHKRLQDGDQGPNTGGMGAYSPAPVVTPQ
LHARIMREVIVPTVEGMAADGIRYTGFLYAGIMVAADGSPKVLEFNCRMGDPETQPIMMRLKSDLVALLEAAVTGRLDQV
DAEWDRRTALAVVLAAAGYPDSPQKGAPLAALPDAAEDLRIFHAGTSAQDGRTVVSGGRVLAVTALGDSVRMAQKRAYDA
VAAIHFDGMQFRRDIGWRALDRSKK

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=424, Percent_Identity=50.9433962264151, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI209869995, Length=424, Percent_Identity=50.9433962264151, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI209869993, Length=424, Percent_Identity=50.9433962264151, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI28416899, Length=424, Percent_Identity=50.9433962264151, Blast_Score=402, Evalue=1e-112,
Organism=Escherichia coli, GI1790438, Length=429, Percent_Identity=62.7039627039627, Blast_Score=532, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI17567511, Length=421, Percent_Identity=42.7553444180523, Blast_Score=326, Evalue=2e-89,
Organism=Saccharomyces cerevisiae, GI6321203, Length=444, Percent_Identity=43.2432432432432, Blast_Score=340, Evalue=3e-94,
Organism=Drosophila melanogaster, GI62471619, Length=431, Percent_Identity=45.0116009280742, Blast_Score=330, Evalue=1e-90,
Organism=Drosophila melanogaster, GI24582400, Length=431, Percent_Identity=45.0116009280742, Blast_Score=329, Evalue=3e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 44445; Mature: 44445

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVIGSGGREHALAWKLAQSPKVSRVFVAPGNGGTAREEGVVNVPISATAELLDFASR
CEEEEEECCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCEEECCHHHHHHHHHHCCC
EDIGLTVVGPEAPLAAGVVDAFRAAGRKIFGPTQAAAQLESSKDFAKQFMARHRIPTAAF
CCCCEEEECCCCCHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHH
ATFSDAAAAHVYIDAQGAPIVVKADGLAAGKGVVVAATADEAHAAVDAMLAGNSMGEAGH
HHHCCCCEEEEEEECCCCEEEEEECCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCC
RVVIEECLVGEEASFIVLVDGEHVLPLASSQDHKRLQDGDQGPNTGGMGAYSPAPVVTPQ
EEHHHHHHCCCCCCEEEEECCCEEEECCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHH
LHARIMREVIVPTVEGMAADGIRYTGFLYAGIMVAADGSPKVLEFNCRMGDPETQPIMMR
HHHHHHHHHHHHCCCCCCCCCEEEHEEEEEEEEEEECCCCCEEEEEECCCCCCCCHHHHH
LKSDLVALLEAAVTGRLDQVDAEWDRRTALAVVLAAAGYPDSPQKGAPLAALPDAAEDLR
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHEEHHEECCCCCCCCCCCCCCEECCCCHHCEE
IFHAGTSAQDGRTVVSGGRVLAVTALGDSVRMAQKRAYDAVAAIHFDGMQFRRDIGWRAL
EEECCCCCCCCCEEEECCEEEEEEECCCHHHHHHHHHHHEEEEEEECCHHHHHHCCCHHH
DRSKK
CCCCC
>Mature Secondary Structure
MKILVIGSGGREHALAWKLAQSPKVSRVFVAPGNGGTAREEGVVNVPISATAELLDFASR
CEEEEEECCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCEEECCHHHHHHHHHHCCC
EDIGLTVVGPEAPLAAGVVDAFRAAGRKIFGPTQAAAQLESSKDFAKQFMARHRIPTAAF
CCCCEEEECCCCCHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHH
ATFSDAAAAHVYIDAQGAPIVVKADGLAAGKGVVVAATADEAHAAVDAMLAGNSMGEAGH
HHHCCCCEEEEEEECCCCEEEEEECCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCC
RVVIEECLVGEEASFIVLVDGEHVLPLASSQDHKRLQDGDQGPNTGGMGAYSPAPVVTPQ
EEHHHHHHCCCCCCEEEEECCCEEEECCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHH
LHARIMREVIVPTVEGMAADGIRYTGFLYAGIMVAADGSPKVLEFNCRMGDPETQPIMMR
HHHHHHHHHHHHCCCCCCCCCEEEHEEEEEEEEEEECCCCCEEEEEECCCCCCCCHHHHH
LKSDLVALLEAAVTGRLDQVDAEWDRRTALAVVLAAAGYPDSPQKGAPLAALPDAAEDLR
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHEEHHEECCCCCCCCCCCCCCEECCCCHHCEE
IFHAGTSAQDGRTVVSGGRVLAVTALGDSVRMAQKRAYDAVAAIHFDGMQFRRDIGWRAL
EEECCCCCCCCCEEEECCEEEEEEECCCHHHHHHHHHHHEEEEEEECCHHHHHHCCCHHH
DRSKK
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]