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The map label for this gene is pilT [H]

Identifier: 74318437

GI number: 74318437

Start: 2490089

End: 2491132

Strand: Direct

Name: pilT [H]

Synonym: Tbd_2419

Alternate gene names: 74318437

Gene position: 2490089-2491132 (Clockwise)

Preceding gene: 74318432

Following gene: 74318438

Centisome position: 85.58

GC content: 62.55

Gene sequence:

>1044_bases
GTGGATATTTCTGAATTGCTCGCTTTCGCCGTCAAAAACAAGGCTTCCGACCTGCACCTTTCCTCGGGTCTGCCGCCGAT
GATCCGCGTGAACGGCGACATCCGCCGCATCAACCTGCCGCCGATGGAGCACAAGGACGTGCACCGCATGGTGTACGACA
TCATGAACGACAGCCAGCGCAAGGTGTACGAGGAGACGCTCGAAATCGACTTCTCGTTCGAGATACCCTCGCTCGCGCGC
TTTCGCGTCAACGCGTTCAACCAACAATACGGCGCGGGCGCCGTGTTCCGCACGATTCCGTCGAAGGTGCTGACGCTCGA
AGAACTGAACTGCCCGCCGATCTTCAAGGAAATCGCCGACCAGCCGCGCGGCATCGTGCTCGTCACCGGGCCGACGGGTT
CGGGCAAGTCGACGACGCTCGCGGCGATGATGGACTACGTCAACGAGAACCAGTATTCGCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTCCACCAGAGCAAGAAATGCCTGATCAACCAGCGCGAGGTCGGGCCGCACACGCTCTCGTTCAACAA
CGCCTTGCGTTCCGCGCTGCGTGAAGACCCCGACGTCATCCTGGTCGGCGAATTGCGCGACCTCGAAACGATCCGCCTCG
CGCTGACCGCTGCCGAGACGGGCCACCTCGTCTTCGGCACGCTCCACACCTCGTCGGCGGCCAAGACGATCGACCGCGTC
GTCGACGTCTTCCCCGCTGCGGAAAAGGAAATGGTCCGCTCGATGCTCTCGGAATCGCTGCGCGCGGTGATTTCGCAGAC
GCTGCTGAAGACCAAGGACGGCACCGGCCGCGTCGCCGCGCACGAAATCATGGTCGGCACCCCGGCGATCCGCAACCTGA
TCCGCGAGAACAAGGTCGCACAGATGTATTCGGCGATCCAGACCGGCGGCAACCTCGGCATGCAGACCCTCGACCAGAAC
CTGCAGGATCTCGTGCGCCGCAACGTCATCGCGTCGAACGTGGCCCGCTCCGCGGCGCAGAACAAAGACGCCTTCATGCT
CTGA

Upstream 100 bases:

>100_bases
TCCCGGCCCCGCTGCAGGCTCAAGTTCGGACGGAAACGGGCCGTTATTCATGGCGTTTATTATCGGTCAAAACCCCACCC
CCATACTGCACGAGGCACGC

Downstream 100 bases:

>100_bases
GGACGAGACGAGACGACATGGCAATGAACGCAGAAAAAACCGTCCACGACCTGCTGCGCCTGATGCTGGCGAAGAACGCG
TCCGACCTTTTCATCACCGC

Product: pilus retraction ATPase PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MDISELLAFAVKNKASDLHLSSGLPPMIRVNGDIRRINLPPMEHKDVHRMVYDIMNDSQRKVYEETLEIDFSFEIPSLAR
FRVNAFNQQYGAGAVFRTIPSKVLTLEELNCPPIFKEIADQPRGIVLVTGPTGSGKSTTLAAMMDYVNENQYSHILTVED
PIEFVHQSKKCLINQREVGPHTLSFNNALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPAAEKEMVRSMLSESLRAVISQTLLKTKDGTGRVAAHEIMVGTPAIRNLIRENKVAQMYSAIQTGGNLGMQTLDQN
LQDLVRRNVIASNVARSAAQNKDAFML

Sequences:

>Translated_347_residues
MDISELLAFAVKNKASDLHLSSGLPPMIRVNGDIRRINLPPMEHKDVHRMVYDIMNDSQRKVYEETLEIDFSFEIPSLAR
FRVNAFNQQYGAGAVFRTIPSKVLTLEELNCPPIFKEIADQPRGIVLVTGPTGSGKSTTLAAMMDYVNENQYSHILTVED
PIEFVHQSKKCLINQREVGPHTLSFNNALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPAAEKEMVRSMLSESLRAVISQTLLKTKDGTGRVAAHEIMVGTPAIRNLIRENKVAQMYSAIQTGGNLGMQTLDQN
LQDLVRRNVIASNVARSAAQNKDAFML
>Mature_347_residues
MDISELLAFAVKNKASDLHLSSGLPPMIRVNGDIRRINLPPMEHKDVHRMVYDIMNDSQRKVYEETLEIDFSFEIPSLAR
FRVNAFNQQYGAGAVFRTIPSKVLTLEELNCPPIFKEIADQPRGIVLVTGPTGSGKSTTLAAMMDYVNENQYSHILTVED
PIEFVHQSKKCLINQREVGPHTLSFNNALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPAAEKEMVRSMLSESLRAVISQTLLKTKDGTGRVAAHEIMVGTPAIRNLIRENKVAQMYSAIQTGGNLGMQTLDQN
LQDLVRRNVIASNVARSAAQNKDAFML

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=325, Percent_Identity=48.9230769230769, Blast_Score=318, Evalue=4e-88,
Organism=Escherichia coli, GI1789723, Length=349, Percent_Identity=33.810888252149, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1786296, Length=208, Percent_Identity=37.9807692307692, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 38495; Mature: 38495

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDISELLAFAVKNKASDLHLSSGLPPMIRVNGDIRRINLPPMEHKDVHRMVYDIMNDSQR
CCHHHHHHHHHHCCCCCEEECCCCCCEEEECCCEEEECCCCCCHHHHHHHHHHHHCCHHH
KVYEETLEIDFSFEIPSLARFRVNAFNQQYGAGAVFRTIPSKVLTLEELNCPPIFKEIAD
HHHHHHHEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHEEHHHCCCCHHHHHHCC
QPRGIVLVTGPTGSGKSTTLAAMMDYVNENQYSHILTVEDPIEFVHQSKKCLINQREVGP
CCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCC
HTLSFNNALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
CEEHHHHHHHHHHHCCCCEEEEECCCCHHHHHEEEEECCCCCEEEEEEECCHHHHHHHHH
VDVFPAAEKEMVRSMLSESLRAVISQTLLKTKDGTGRVAAHEIMVGTPAIRNLIRENKVA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHEEECCHHHHHHHHHHHHH
QMYSAIQTGGNLGMQTLDQNLQDLVRRNVIASNVARSAAQNKDAFML
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDISELLAFAVKNKASDLHLSSGLPPMIRVNGDIRRINLPPMEHKDVHRMVYDIMNDSQR
CCHHHHHHHHHHCCCCCEEECCCCCCEEEECCCEEEECCCCCCHHHHHHHHHHHHCCHHH
KVYEETLEIDFSFEIPSLARFRVNAFNQQYGAGAVFRTIPSKVLTLEELNCPPIFKEIAD
HHHHHHHEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHEEHHHCCCCHHHHHHCC
QPRGIVLVTGPTGSGKSTTLAAMMDYVNENQYSHILTVEDPIEFVHQSKKCLINQREVGP
CCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCC
HTLSFNNALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
CEEHHHHHHHHHHHCCCCEEEEECCCCHHHHHEEEEECCCCCEEEEEEECCHHHHHHHHH
VDVFPAAEKEMVRSMLSESLRAVISQTLLKTKDGTGRVAAHEIMVGTPAIRNLIRENKVA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHEEECCHHHHHHHHHHHHH
QMYSAIQTGGNLGMQTLDQNLQDLVRRNVIASNVARSAAQNKDAFML
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]