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The map label for this gene is pilT [H]

Identifier: 74318438

GI number: 74318438

Start: 2491150

End: 2492283

Strand: Direct

Name: pilT [H]

Synonym: Tbd_2420

Alternate gene names: 74318438

Gene position: 2491150-2492283 (Clockwise)

Preceding gene: 74318437

Following gene: 74318444

Centisome position: 85.61

GC content: 61.64

Gene sequence:

>1134_bases
ATGGCAATGAACGCAGAAAAAACCGTCCACGACCTGCTGCGCCTGATGCTGGCGAAGAACGCGTCCGACCTTTTCATCAC
CGCGGGATTCCCGCCCGCGATCAAGGTCGACGGCAAGATCACCCCGGTCTCGAACCAGAGCCTGACGCCCGCGCACACGA
GCGAGCTCGCGCGCTCGATCATGAACGATCGCCAGTGGAAAGATTTCGAGGCGACCAACGAATCGAACTTCGCGATCAGC
CCGCCCGAGATCGGGCGCTTCCGCGTCAACGTCTTCGTGCAACAGGGCCGCGTCGGCATCGTCATGCGGACGATCAATAC
CAAGATCCCGAAGATGGAAGACCTCAACCTGCCGCCGATCCTGCGCGACGTCGCGATGATCAAGCGCGGCCTCGTCGTCT
TCGTCGGCGGCACCGGCACCGGTAAATCGACCTCGCTCGCCGCGATGGTCGGCTACCGCAACGAGAACGACGCCGGCCAC
ATCATCACCGTCGAGGACCCGATCGAATACGTGCACGAGCACCGCAAATCGATCGTCAGCCAGCGCGAGGTCGGCATCGA
CACCGACAACTGGTTCTCCGCGCTCAAGAACACGCTGCGCCAGGCGCCCGACGTCATCCTGATCGGCGAAATCCGCGACC
GGGAGACCATGGAATACGCGATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGTCTACGCTGCACGCCAACAGCGCCAAC
CAGGCGCTCGACCGCATCATCAACTTCTTCCCCGAGGAGAAGCGCGATCAATTGCTGATGGACTTGTCGCTCAACCTCAA
GTCCTTCATCTCGCAGCGCCTGATTCCGCGAAAAGGCAGCAACGGCCGCGTCGCCGCCGTCGAAATCCTGCTCAACTCGC
CGCTCATCGCCGACCTGATCTTCAAGGGCCACGTCCACGAGATCAAGGAGATCATGGCCAAGTCCCGCGAACTCGGCATG
CAGACCTTCGACCAGGCCCTGTTCGACCTCTACGAAGCCGGACTGATCTCCTACGAGGACGCGCTAAGAAACGCCGACAG
CCTCAACGACCTACGCCTGCAGATCAAGCTGCACGGCCAGGAGGCCAAGGGGCGGGACATGATGGCGGGGTTGGATCATC
TCGATATTGTTTAA

Upstream 100 bases:

>100_bases
TGCAGGATCTCGTGCGCCGCAACGTCATCGCGTCGAACGTGGCCCGCTCCGCGGCGCAGAACAAAGACGCCTTCATGCTC
TGAGGACGAGACGAGACGAC

Downstream 100 bases:

>100_bases
GTCGTCGGGAGTGCAAAACAAAACGGGGTGTTTAGCACCCCATTTTGTCTTGTCAACGCCGGCTCGGTGCCGGCGTAGCC
TCGCATCAAACTTGTTGTTA

Product: pilus retraction protein PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MAMNAEKTVHDLLRLMLAKNASDLFITAGFPPAIKVDGKITPVSNQSLTPAHTSELARSIMNDRQWKDFEATNESNFAIS
PPEIGRFRVNVFVQQGRVGIVMRTINTKIPKMEDLNLPPILRDVAMIKRGLVVFVGGTGTGKSTSLAAMVGYRNENDAGH
IITVEDPIEYVHEHRKSIVSQREVGIDTDNWFSALKNTLRQAPDVILIGEIRDRETMEYAIAFAETGHLCLSTLHANSAN
QALDRIINFFPEEKRDQLLMDLSLNLKSFISQRLIPRKGSNGRVAAVEILLNSPLIADLIFKGHVHEIKEIMAKSRELGM
QTFDQALFDLYEAGLISYEDALRNADSLNDLRLQIKLHGQEAKGRDMMAGLDHLDIV

Sequences:

>Translated_377_residues
MAMNAEKTVHDLLRLMLAKNASDLFITAGFPPAIKVDGKITPVSNQSLTPAHTSELARSIMNDRQWKDFEATNESNFAIS
PPEIGRFRVNVFVQQGRVGIVMRTINTKIPKMEDLNLPPILRDVAMIKRGLVVFVGGTGTGKSTSLAAMVGYRNENDAGH
IITVEDPIEYVHEHRKSIVSQREVGIDTDNWFSALKNTLRQAPDVILIGEIRDRETMEYAIAFAETGHLCLSTLHANSAN
QALDRIINFFPEEKRDQLLMDLSLNLKSFISQRLIPRKGSNGRVAAVEILLNSPLIADLIFKGHVHEIKEIMAKSRELGM
QTFDQALFDLYEAGLISYEDALRNADSLNDLRLQIKLHGQEAKGRDMMAGLDHLDIV
>Mature_376_residues
AMNAEKTVHDLLRLMLAKNASDLFITAGFPPAIKVDGKITPVSNQSLTPAHTSELARSIMNDRQWKDFEATNESNFAISP
PEIGRFRVNVFVQQGRVGIVMRTINTKIPKMEDLNLPPILRDVAMIKRGLVVFVGGTGTGKSTSLAAMVGYRNENDAGHI
ITVEDPIEYVHEHRKSIVSQREVGIDTDNWFSALKNTLRQAPDVILIGEIRDRETMEYAIAFAETGHLCLSTLHANSANQ
ALDRIINFFPEEKRDQLLMDLSLNLKSFISQRLIPRKGSNGRVAAVEILLNSPLIADLIFKGHVHEIKEIMAKSRELGMQ
TFDQALFDLYEAGLISYEDALRNADSLNDLRLQIKLHGQEAKGRDMMAGLDHLDIV

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=324, Percent_Identity=36.1111111111111, Blast_Score=227, Evalue=8e-61,
Organism=Escherichia coli, GI1789723, Length=164, Percent_Identity=39.0243902439024, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1786296, Length=296, Percent_Identity=29.7297297297297, Blast_Score=95, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 42095; Mature: 41964

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMNAEKTVHDLLRLMLAKNASDLFITAGFPPAIKVDGKITPVSNQSLTPAHTSELARSI
CCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEECCEEECCCCCCCCCHHHHHHHHHH
MNDRQWKDFEATNESNFAISPPEIGRFRVNVFVQQGRVGIVMRTINTKIPKMEDLNLPPI
HCCCCCCCCCCCCCCCEEECCCCCCEEEEEEEEECCCEEEEEEECCCCCCCCCCCCCCHH
LRDVAMIKRGLVVFVGGTGTGKSTSLAAMVGYRNENDAGHIITVEDPIEYVHEHRKSIVS
HHHHHHHHCCEEEEECCCCCCCCCEEEEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHH
QREVGIDTDNWFSALKNTLRQAPDVILIGEIRDRETMEYAIAFAETGHLCLSTLHANSAN
HHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHCCCHH
QALDRIINFFPEEKRDQLLMDLSLNLKSFISQRLIPRKGSNGRVAAVEILLNSPLIADLI
HHHHHHHHHCCCCCHHHEEHHHCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHH
FKGHVHEIKEIMAKSRELGMQTFDQALFDLYEAGLISYEDALRNADSLNDLRLQIKLHGQ
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEEEEEECC
EAKGRDMMAGLDHLDIV
CCCCCHHHHCCHHHCCC
>Mature Secondary Structure 
AMNAEKTVHDLLRLMLAKNASDLFITAGFPPAIKVDGKITPVSNQSLTPAHTSELARSI
CCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEECCEEECCCCCCCCCHHHHHHHHHH
MNDRQWKDFEATNESNFAISPPEIGRFRVNVFVQQGRVGIVMRTINTKIPKMEDLNLPPI
HCCCCCCCCCCCCCCCEEECCCCCCEEEEEEEEECCCEEEEEEECCCCCCCCCCCCCCHH
LRDVAMIKRGLVVFVGGTGTGKSTSLAAMVGYRNENDAGHIITVEDPIEYVHEHRKSIVS
HHHHHHHHCCEEEEECCCCCCCCCEEEEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHH
QREVGIDTDNWFSALKNTLRQAPDVILIGEIRDRETMEYAIAFAETGHLCLSTLHANSAN
HHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHCCCHH
QALDRIINFFPEEKRDQLLMDLSLNLKSFISQRLIPRKGSNGRVAAVEILLNSPLIADLI
HHHHHHHHHCCCCCHHHEEHHHCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHH
FKGHVHEIKEIMAKSRELGMQTFDQALFDLYEAGLISYEDALRNADSLNDLRLQIKLHGQ
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEEEEEECC
EAKGRDMMAGLDHLDIV
CCCCCHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]