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The map label for this gene is 74318426

Identifier: 74318426

GI number: 74318426

Start: 2479620

End: 2480900

Strand: Direct

Name: 74318426

Synonym: Tbd_2408

Alternate gene names: NA

Gene position: 2479620-2480900 (Clockwise)

Preceding gene: 74318422

Following gene: 74318427

Centisome position: 85.22

GC content: 63.47

Gene sequence:

>1281_bases
ATGGTTCCTTACCTCACGACCGCCCTCACCGGGCCCTTGCTGGAACTCGAAAAACGCCTGCTCGACGCGCAGCCGACGAT
CGAGCACTGGTTCCGCCAGCAATGGAAGGAGCAGGCCGCGCCCTTCTATACCTCGGTCGACATCCGCAACGCGGGCTTCA
AGCTCGCGCCCGTCGACACCAATCTCTTTCCAGGCGGCTTCAACAATCTCAACCCGTCGTTCATGTCGCTGTCGATCCAT
GCGGCGATGGGTGCCGTCGAAAAGATCTGCCCCGACGCGCAGCGCCTGCTGCTGATCCCCGAGAATCACACGCGCAACAG
CTTCTATCTGCAAAACGTCGCCGTGCTCGCGCACATCCTGCGCCAGACCGGGCTCATCGTGCGTATCGGCACGCTGATTC
CGGACATCACCGAGCCGACCACGCTGCAGCTTCCGGGCGGCGCGCAGCTGACGCTCGAGCCGCTGGTGCGCACGGGCGAC
CGCATCGGCCTCGACGGCTTCGATCCCTGCGCGGTATTGCTCAACAACGACCTCTCCGCGGGCGCGCCCGAGATTCTCAA
GGGCATCGAACAGACGATCATGCCGCCTTTACACGCGGGCTGGGCGACGCGCCGCAAGTCGCGACATTTCAGGGCCTACG
AAAACGTCGCCATCGAGTTCGCGCGCCTGATCGACATCGACCCCTGGCTGATCGACCCCTATTTCACGCATTGCGGCAAG
ATCGATTTCCAGGCGCGCCAGGGCGAGGACTGCCTCGCGGAGAAAGTCGGGCAGATGCTCGAGAAAATCCGCGCCAAGTA
CGACGAATACGGCATCGACCAGCCGCCCTTCGTCATCGTCAAGGCCGACGCCGGCACCTACGGCATGGGCATCATGACCG
TGAAGTCGCCCGATGACGTGCGCGAACTCAACCGCAAGCAGCGCAACAAGATGGCCGTCATCAAGGAAGGGCTCGAAGTC
AGCGAGGTCCTGATCCAGGAAGGCGTCTACACCTTCGAGAGCATCAACGAAGCGGTTGCCGAACCGGTCGTCTACATGCT
CGACCATTTCGTCGTCGGCGGTTTCTATCGCGTCCACACCGGACGCGGCGCCGACGAGAACCTCAACAGCCCGGGCATGC
ATTTCGTGCCGCTCGCGTTCGACGACACCTGCGTGATGCCCGACAGCAACGCGAGCCCCGACGCCAGCCCCAACCGCTTC
TACGCCTACGGCGTCGTCGCGCGCCTGGCGATGCTCGCCGCCTCGATCGAACTCGACGAAGCCTTGAACGAGACGGCATG
A

Upstream 100 bases:

>100_bases
ATCAGATTGCCGGTATTGGGCGGGATTTCGGGCTGATATAAAACAATGTCAAACATCGGGCTTGCCCTAAAATAAAACAT
TCTCGGAGTCCGCCTATTCT

Downstream 100 bases:

>100_bases
AGCTGCTGTTCGTCGTCGATCCGCTGGCGAGCCTCAAGCCGTACAAGGACAGTTCGGTCGCGATGATGCGCGCGGCCTGT
GCGCGCGGCCACGCCGTGTT

Product: glutamate--cysteine ligase GshA

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Glutamate-Cysteine Ligase GshA; Glutamate-Cysteine Ligase-Related Protein; Glutathione Synthetase; GshA Protein

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MVPYLTTALTGPLLELEKRLLDAQPTIEHWFRQQWKEQAAPFYTSVDIRNAGFKLAPVDTNLFPGGFNNLNPSFMSLSIH
AAMGAVEKICPDAQRLLLIPENHTRNSFYLQNVAVLAHILRQTGLIVRIGTLIPDITEPTTLQLPGGAQLTLEPLVRTGD
RIGLDGFDPCAVLLNNDLSAGAPEILKGIEQTIMPPLHAGWATRRKSRHFRAYENVAIEFARLIDIDPWLIDPYFTHCGK
IDFQARQGEDCLAEKVGQMLEKIRAKYDEYGIDQPPFVIVKADAGTYGMGIMTVKSPDDVRELNRKQRNKMAVIKEGLEV
SEVLIQEGVYTFESINEAVAEPVVYMLDHFVVGGFYRVHTGRGADENLNSPGMHFVPLAFDDTCVMPDSNASPDASPNRF
YAYGVVARLAMLAASIELDEALNETA

Sequences:

>Translated_426_residues
MVPYLTTALTGPLLELEKRLLDAQPTIEHWFRQQWKEQAAPFYTSVDIRNAGFKLAPVDTNLFPGGFNNLNPSFMSLSIH
AAMGAVEKICPDAQRLLLIPENHTRNSFYLQNVAVLAHILRQTGLIVRIGTLIPDITEPTTLQLPGGAQLTLEPLVRTGD
RIGLDGFDPCAVLLNNDLSAGAPEILKGIEQTIMPPLHAGWATRRKSRHFRAYENVAIEFARLIDIDPWLIDPYFTHCGK
IDFQARQGEDCLAEKVGQMLEKIRAKYDEYGIDQPPFVIVKADAGTYGMGIMTVKSPDDVRELNRKQRNKMAVIKEGLEV
SEVLIQEGVYTFESINEAVAEPVVYMLDHFVVGGFYRVHTGRGADENLNSPGMHFVPLAFDDTCVMPDSNASPDASPNRF
YAYGVVARLAMLAASIELDEALNETA
>Mature_426_residues
MVPYLTTALTGPLLELEKRLLDAQPTIEHWFRQQWKEQAAPFYTSVDIRNAGFKLAPVDTNLFPGGFNNLNPSFMSLSIH
AAMGAVEKICPDAQRLLLIPENHTRNSFYLQNVAVLAHILRQTGLIVRIGTLIPDITEPTTLQLPGGAQLTLEPLVRTGD
RIGLDGFDPCAVLLNNDLSAGAPEILKGIEQTIMPPLHAGWATRRKSRHFRAYENVAIEFARLIDIDPWLIDPYFTHCGK
IDFQARQGEDCLAEKVGQMLEKIRAKYDEYGIDQPPFVIVKADAGTYGMGIMTVKSPDDVRELNRKQRNKMAVIKEGLEV
SEVLIQEGVYTFESINEAVAEPVVYMLDHFVVGGFYRVHTGRGADENLNSPGMHFVPLAFDDTCVMPDSNASPDASPNRF
YAYGVVARLAMLAASIELDEALNETA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 47298; Mature: 47298

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPYLTTALTGPLLELEKRLLDAQPTIEHWFRQQWKEQAAPFYTSVDIRNAGFKLAPVDT
CCCCHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEECCC
NLFPGGFNNLNPSFMSLSIHAAMGAVEKICPDAQRLLLIPENHTRNSFYLQNVAVLAHIL
CCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHH
RQTGLIVRIGTLIPDITEPTTLQLPGGAQLTLEPLVRTGDRIGLDGFDPCAVLLNNDLSA
HHCCCEEEEHHHCCCCCCCCEEECCCCCCEEHHHHHHCCCCCCCCCCCHHHHEECCCCCC
GAPEILKGIEQTIMPPLHAGWATRRKSRHFRAYENVAIEFARLIDIDPWLIDPYFTHCGK
CCHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHCCC
IDFQARQGEDCLAEKVGQMLEKIRAKYDEYGIDQPPFVIVKADAGTYGMGIMTVKSPDDV
EEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCHHH
RELNRKQRNKMAVIKEGLEVSEVLIQEGVYTFESINEAVAEPVVYMLDHFVVGGFYRVHT
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEC
GRGADENLNSPGMHFVPLAFDDTCVMPDSNASPDASPNRFYAYGVVARLAMLAASIELDE
CCCCCCCCCCCCCEEEEEEECCEEECCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHH
ALNETA
HHCCCC
>Mature Secondary Structure
MVPYLTTALTGPLLELEKRLLDAQPTIEHWFRQQWKEQAAPFYTSVDIRNAGFKLAPVDT
CCCCHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEECCC
NLFPGGFNNLNPSFMSLSIHAAMGAVEKICPDAQRLLLIPENHTRNSFYLQNVAVLAHIL
CCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHH
RQTGLIVRIGTLIPDITEPTTLQLPGGAQLTLEPLVRTGDRIGLDGFDPCAVLLNNDLSA
HHCCCEEEEHHHCCCCCCCCEEECCCCCCEEHHHHHHCCCCCCCCCCCHHHHEECCCCCC
GAPEILKGIEQTIMPPLHAGWATRRKSRHFRAYENVAIEFARLIDIDPWLIDPYFTHCGK
CCHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHCCC
IDFQARQGEDCLAEKVGQMLEKIRAKYDEYGIDQPPFVIVKADAGTYGMGIMTVKSPDDV
EEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEECCCHHH
RELNRKQRNKMAVIKEGLEVSEVLIQEGVYTFESINEAVAEPVVYMLDHFVVGGFYRVHT
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEC
GRGADENLNSPGMHFVPLAFDDTCVMPDSNASPDASPNRFYAYGVVARLAMLAASIELDE
CCCCCCCCCCCCCEEEEEEECCEEECCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHH
ALNETA
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA