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The map label for this gene is dnaG [H]

Identifier: 74318405

GI number: 74318405

Start: 2458278

End: 2459975

Strand: Direct

Name: dnaG [H]

Synonym: Tbd_2387

Alternate gene names: 74318405

Gene position: 2458278-2459975 (Clockwise)

Preceding gene: 74318404

Following gene: 74318406

Centisome position: 84.48

GC content: 64.96

Gene sequence:

>1698_bases
ATGATTCCGCGCGATTTCATCGATACCCTGCTGAGCCGCGTCGACATTGTCGACGTGATTGACCGACGGGTTCCCCTCAA
GAAAGCCGGCCAGAACTACCAGGCCTGCTGCCCCTTTCACAGCGAAAAGACGCCGTCCTTCACGGTCAGTCCCAGCAAGC
AGTTCTACCACTGCTTCGGTTGCGGCGCCCACGGCACCGCGCTCGGCTTCCTAATGGAATACGAGCACATGAGCTTTCCC
GACTCCGTCGCCGCGCTTGCCGAGGACGCGGGCCTGCCGATGCCGGAATCGAGCCTCGAACCCGAGCGTCCGAAACCACC
GCCAGCGCTGTGGGACGCGCTAGAACAAGCGGCACGCTTCTACAAGCAGCAACTCAAGCAGGCACCGCATGCGATCGACT
ACCTCAAGCAGCGCGGCCTGAGCGGCGCCATCGCCGCGCGCTACGGTATCGGTTACGCTCCCGACGGATCGCCGCTCAAA
CGCGTTTTCGCCGACTACGACGCAGAGGCGCTCGCCGCCTCGGGACTGGTCGTCGACAACGAAGGCCGACGCTACGACCG
CTTCCGCGACCGCATCATGTTTCCCATCCGCAACGTCAAGGGTCAGATCGTCGGCTTCGGCGGCCGGGTGCTCGGGCAGG
GCGAGCCGAAATACCTGAACTCCCCGGAAACGCCGCTGTTTCACAAAGGCTCGGAAATCTACGGGCTGTTCGAAGCGCGT
GCCGCGATCAAGCGCGCGGCGCGGGTCGTCGTCGTCGAGGGCTATATGGACGTCGTGGCGCTCGCGCAGCACGGCGTCGA
ATTCGCCGTCGCGACGCTCGGCACGGCGACGACGCCCGTCCACGCGCGCACGCTTCTGCGCCATAGCGACCGGCTTGTCT
ACGCCTTCGATGGCGACGCCGCCGGGCGCAAGGCCGCGTGGCGGGCGCTGGAAAACAGCCTCGAAGCGCTGCAGGACGGC
AAGGAAGTCAGCTTCCTGTTCCTGCCTGAAGGCGAGGATCCGGACAGCTACATCCGCGACCACGGCTGCGACGCCTTTCT
GCGACTCCTCGACAATGAAACCTTGCCCTTGTCGGCGTTCCTGATGCGCGAACTCGCGCGCGGCCGACGACTCGACACTC
AGGAAGGCCAGGCGACCCTGTTGAAGGACGCCAAGCCGCTGCTGGAAAAAATCGGCGCACCGCTGCTCGCACGCCTCATT
CAGCGTCAAATCGCGGAGTTGACGCAACTTCAGGTCGGCGATCTCAGCGCAATGGGCCTGCGACCGGTCGCGTCCCGCCC
GCTTCCGCCGCGCCAACAACGCCGCATCGCCCCTTCGCGCCTCCGGGGTTTGGCACGCATTCTGCTAATGAATCCGCAGC
GGGCAAGCGAAGTCGAGCCGCTTTGGTTCGATGCGCGCGATCCGCTCCATGCGACGGCAGGCGAACTGATCGCCTGGCTG
CGCGAAGTGGGGCCGCTCAGGCTGCACACATTGGCGGAAGCCGCGAAGGGCAGCGGCATGGAAGACTTCGTCAATGAACT
GATGGTCGATTTACTCGACAAAGATGACACCTGGGACTGGAACGCGGAGTTCGAAGGTGCCGTCCAACAACTACGCGACG
ACTGGCGCCGCCGCCGGGTGCAGGAATTGGCGGCCCGTCCGCTCGCCAGCCTGTCGGTCGAAGAGCGCCGCGAACTGGGT
GACTTGGCACGCTCGTAG

Upstream 100 bases:

>100_bases
GCCGACATGGCGCAGGTTTCCGCGCTCATCAAGGCGCGCTTGGCAGGCTGACCCTTCGCCCGGCCCAGCCCCAGCCGGCC
GGGTCTATCATGCGGTAAAC

Downstream 100 bases:

>100_bases
CTTGCTGGGCGCGCCGGGACATTGCCCCGTTTTACGGTATAATGAGCGGTTTTGAATGATTTTTTCCGGCGCGGTCCATA
GCGTAACGGAAAACATAAGC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 565; Mature: 565

Protein sequence:

>565_residues
MIPRDFIDTLLSRVDIVDVIDRRVPLKKAGQNYQACCPFHSEKTPSFTVSPSKQFYHCFGCGAHGTALGFLMEYEHMSFP
DSVAALAEDAGLPMPESSLEPERPKPPPALWDALEQAARFYKQQLKQAPHAIDYLKQRGLSGAIAARYGIGYAPDGSPLK
RVFADYDAEALAASGLVVDNEGRRYDRFRDRIMFPIRNVKGQIVGFGGRVLGQGEPKYLNSPETPLFHKGSEIYGLFEAR
AAIKRAARVVVVEGYMDVVALAQHGVEFAVATLGTATTPVHARTLLRHSDRLVYAFDGDAAGRKAAWRALENSLEALQDG
KEVSFLFLPEGEDPDSYIRDHGCDAFLRLLDNETLPLSAFLMRELARGRRLDTQEGQATLLKDAKPLLEKIGAPLLARLI
QRQIAELTQLQVGDLSAMGLRPVASRPLPPRQQRRIAPSRLRGLARILLMNPQRASEVEPLWFDARDPLHATAGELIAWL
REVGPLRLHTLAEAAKGSGMEDFVNELMVDLLDKDDTWDWNAEFEGAVQQLRDDWRRRRVQELAARPLASLSVEERRELG
DLARS

Sequences:

>Translated_565_residues
MIPRDFIDTLLSRVDIVDVIDRRVPLKKAGQNYQACCPFHSEKTPSFTVSPSKQFYHCFGCGAHGTALGFLMEYEHMSFP
DSVAALAEDAGLPMPESSLEPERPKPPPALWDALEQAARFYKQQLKQAPHAIDYLKQRGLSGAIAARYGIGYAPDGSPLK
RVFADYDAEALAASGLVVDNEGRRYDRFRDRIMFPIRNVKGQIVGFGGRVLGQGEPKYLNSPETPLFHKGSEIYGLFEAR
AAIKRAARVVVVEGYMDVVALAQHGVEFAVATLGTATTPVHARTLLRHSDRLVYAFDGDAAGRKAAWRALENSLEALQDG
KEVSFLFLPEGEDPDSYIRDHGCDAFLRLLDNETLPLSAFLMRELARGRRLDTQEGQATLLKDAKPLLEKIGAPLLARLI
QRQIAELTQLQVGDLSAMGLRPVASRPLPPRQQRRIAPSRLRGLARILLMNPQRASEVEPLWFDARDPLHATAGELIAWL
REVGPLRLHTLAEAAKGSGMEDFVNELMVDLLDKDDTWDWNAEFEGAVQQLRDDWRRRRVQELAARPLASLSVEERRELG
DLARS
>Mature_565_residues
MIPRDFIDTLLSRVDIVDVIDRRVPLKKAGQNYQACCPFHSEKTPSFTVSPSKQFYHCFGCGAHGTALGFLMEYEHMSFP
DSVAALAEDAGLPMPESSLEPERPKPPPALWDALEQAARFYKQQLKQAPHAIDYLKQRGLSGAIAARYGIGYAPDGSPLK
RVFADYDAEALAASGLVVDNEGRRYDRFRDRIMFPIRNVKGQIVGFGGRVLGQGEPKYLNSPETPLFHKGSEIYGLFEAR
AAIKRAARVVVVEGYMDVVALAQHGVEFAVATLGTATTPVHARTLLRHSDRLVYAFDGDAAGRKAAWRALENSLEALQDG
KEVSFLFLPEGEDPDSYIRDHGCDAFLRLLDNETLPLSAFLMRELARGRRLDTQEGQATLLKDAKPLLEKIGAPLLARLI
QRQIAELTQLQVGDLSAMGLRPVASRPLPPRQQRRIAPSRLRGLARILLMNPQRASEVEPLWFDARDPLHATAGELIAWL
REVGPLRLHTLAEAAKGSGMEDFVNELMVDLLDKDDTWDWNAEFEGAVQQLRDDWRRRRVQELAARPLASLSVEERRELG
DLARS

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=582, Percent_Identity=41.9243986254296, Blast_Score=407, Evalue=1e-114,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 62975; Mature: 62975

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPRDFIDTLLSRVDIVDVIDRRVPLKKAGQNYQACCPFHSEKTPSFTVSPSKQFYHCFG
CCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCEECCCCCCCCCCEEECCCHHEEEEEC
CGAHGTALGFLMEYEHMSFPDSVAALAEDAGLPMPESSLEPERPKPPPALWDALEQAARF
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
YKQQLKQAPHAIDYLKQRGLSGAIAARYGIGYAPDGSPLKRVFADYDAEALAASGLVVDN
HHHHHHHCCHHHHHHHHCCCCCHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHCCCEECC
EGRRYDRFRDRIMFPIRNVKGQIVGFGGRVLGQGEPKYLNSPETPLFHKGSEIYGLFEAR
CCCHHHHHHHHHHHHHHCCCCEEEEECCEEECCCCCCCCCCCCCCCEECCCHHHHHHHHH
AAIKRAARVVVVEGYMDVVALAQHGVEFAVATLGTATTPVHARTLLRHSDRLVYAFDGDA
HHHHHHHEEEEEHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCC
AGRKAAWRALENSLEALQDGKEVSFLFLPEGEDPDSYIRDHGCDAFLRLLDNETLPLSAF
CCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCHHHHHHHHCCCCCCHHHH
LMRELARGRRLDTQEGQATLLKDAKPLLEKIGAPLLARLIQRQIAELTQLQVGDLSAMGL
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHCCC
RPVASRPLPPRQQRRIAPSRLRGLARILLMNPQRASEVEPLWFDARDPLHATAGELIAWL
CCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCHHHHHHHHHHH
REVGPLRLHTLAEAAKGSGMEDFVNELMVDLLDKDDTWDWNAEFEGAVQQLRDDWRRRRV
HHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
QELAARPLASLSVEERRELGDLARS
HHHHHCCHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure
MIPRDFIDTLLSRVDIVDVIDRRVPLKKAGQNYQACCPFHSEKTPSFTVSPSKQFYHCFG
CCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCEECCCCCCCCCCEEECCCHHEEEEEC
CGAHGTALGFLMEYEHMSFPDSVAALAEDAGLPMPESSLEPERPKPPPALWDALEQAARF
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
YKQQLKQAPHAIDYLKQRGLSGAIAARYGIGYAPDGSPLKRVFADYDAEALAASGLVVDN
HHHHHHHCCHHHHHHHHCCCCCHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHCCCEECC
EGRRYDRFRDRIMFPIRNVKGQIVGFGGRVLGQGEPKYLNSPETPLFHKGSEIYGLFEAR
CCCHHHHHHHHHHHHHHCCCCEEEEECCEEECCCCCCCCCCCCCCCEECCCHHHHHHHHH
AAIKRAARVVVVEGYMDVVALAQHGVEFAVATLGTATTPVHARTLLRHSDRLVYAFDGDA
HHHHHHHEEEEEHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCC
AGRKAAWRALENSLEALQDGKEVSFLFLPEGEDPDSYIRDHGCDAFLRLLDNETLPLSAF
CCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCHHHHHHHHCCCCCCHHHH
LMRELARGRRLDTQEGQATLLKDAKPLLEKIGAPLLARLIQRQIAELTQLQVGDLSAMGL
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHCCC
RPVASRPLPPRQQRRIAPSRLRGLARILLMNPQRASEVEPLWFDARDPLHATAGELIAWL
CCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCHHHHHHHHHHH
REVGPLRLHTLAEAAKGSGMEDFVNELMVDLLDKDDTWDWNAEFEGAVQQLRDDWRRRRV
HHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
QELAARPLASLSVEERRELGDLARS
HHHHHCCHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]