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The map label for this gene is rpoD [H]

Identifier: 74318406

GI number: 74318406

Start: 2460031

End: 2462001

Strand: Direct

Name: rpoD [H]

Synonym: Tbd_2388

Alternate gene names: 74318406

Gene position: 2460031-2462001 (Clockwise)

Preceding gene: 74318405

Following gene: 74318407

Centisome position: 84.54

GC content: 62.35

Gene sequence:

>1971_bases
ATGATTTTTTCCGGCGCGGTCCATAGCGTAACGGAAAACATAAGCGGAGAAAAGCCTGTGGCTGTACGTGGAAGAAAACC
CGGCGGCGGCGCCAAGAAACCCGAGGCACTGCTCCCCCTGAAGGAGTTGACGCCCGTCGAGGCCGAGGCACGCCGTACCC
AGCTGAAGAATCTCATCATTCTGGGCAAGGAGCGCGGCTTTCTGACGTACGCCGAGATCAACGATCACCTGCCCGACGAG
GTGCTGGACGCCGACCAGATCGAAGGCGTGATCAGCATGATCAACGACATGGGCATTCAGGTCTACGACGAAGCGCCGGA
CGCCGAGACGCTGCTCATGCAGGAAGCGGCGCCGGCTGTCGCGGACGAGGACGTCGTCGCAGAGGCCGAGCAGGCGCTGA
CGACGGTCGATTCCGAATTCGGGCGGACGACCGACCCCGTGCGCATGTACATGCGCGAAATGGGCTCGGTCGACCTGCTC
ACGCGCGAGGGCGAAATCGAGATCGCGAAGCGCATCGAGGAAGGCCTGCGCCATATGGTGCAGGCGATTTCGTCGTGCCC
GCTGACGATCCAGCAGATCCTCGACATGGCGGCCCAGGTCGAGAAGGACGAACTGCGCATCGACGAGTTGATCGATGGTT
TCGTCGAGGCGGAAACCGAAGCTGCCGCCGAGGAGGAAACCGCGGCGGGCGACGATGAAACCGACGAGGACGACGAGGAC
GCGAGCGCCGCCGTGGCCGCCGCCAACCTTGCGCAACTCAAGGCCGAGGCGCTCGCGCAGTTCGACTACATCCGCAAGGC
CTACAAAAAGGCGCAGAACGCCCACGTCAAATATGGACTCGGCAGCGCGCAGCACATGAAGGCACAGGCCGAAGTCACCG
AACTGCTGATGGCGATCCGCTTCTCGGTGCGCCAGGTGGACAACCTTTGCGAGCAGATCCGCAGCGCGGTCGAAGAAGTG
CGCTCGCACGAGCGCAAGATCATGGAGTTTTGCGTAAACCGCTCGGGCATGCCGCGGGCGCACTTCATCAAGGCCTTCCC
CGGCAACGAGACCAATCTCGCCTGGCTGGACAGGGAAATCGCCGCCAAGAAAGCCTACTGCTCGACGCTCTCGAAAGTCC
AGTACGAGGTCAAGGCGCACCAGGAGGCCTTGATCGCGATGCAGGACCGGGTCGGCCTGCCGATCAAGAATCTCAAGGAC
ATCAACAAGCAGATGTCGACCGGCGAAGCCAAGGCGCGGCGCGCGAAGCGCGAGATGATCGAGGCGAACCTGCGGCTGGT
GATCTCGATCGCGAAGAAGTACACCAACCGCGGCCTGCAGTTCCTCGACCTGATCCAGGAAGGCAACATCGGCCTCATGA
AGGCCGTGGACAAATTCGAATACCGCCGCGGCTACAAGTTCTCGACCTACGCGACCTGGTGGATCCGGCAGGCGATCACG
CGCTCGATCGCGGACCAGGCCCGCACGATCCGCATCCCGGTACACATGATCGAGACGATCAACAAGATGAACCGGATCAG
CCGGCAGATTCTGCAGGAAACGGGCATCGAGCCGGACCCCGCGACGCTCGCGATCAAGATGGAAATGCCGGAAGACAAGA
TCCGCAAGATCATGAAGATCGCGAAGGAGCCGATCTCGATGGAGACACCGATCGGCGACGACGAGGATTCCCATCTCGGC
GACTTCATCGAGGACGCGAGCACGCTCTCGCCCGACGACTCCGCGATCTACGCCAACCTGCGCGACGCCACGCGGGAGGT
GCTCGACAGCCTGACCTCGCGCGAAGCCAAGGTCCTGCGCATGCGCTTCGGTATCGAGATGAACACCGACCACACACTCG
AGGAAGTCGGCAAGCAGTTCGACGTGACCCGCGAGCGCATTCGGCAGATCGAAGCGAAGGCCCTGCGCAAGCTGCGCCAT
CCGACCCGCTCCGAAAAGCTGAAGAGCTTCGTCGGCAGCGGCGAACAGTAA

Upstream 100 bases:

>100_bases
TCGGTCGAAGAGCGCCGCGAACTGGGTGACTTGGCACGCTCGTAGCTTGCTGGGCGCGCCGGGACATTGCCCCGTTTTAC
GGTATAATGAGCGGTTTTGA

Downstream 100 bases:

>100_bases
AGCCCCGCCGCCCGGCGGGGCTTCCACGTTTTCGGGTCTGTAGCTCAGCTGGTTAGAGCAGGGGACTCATAATCCCTTGG
TCCACGGTTCAAGTCCGTGC

Product: carbamoyl-phosphate synthase L chain, ATP-binding

Products: NA

Alternate protein names: Sigma-70 [H]

Number of amino acids: Translated: 656; Mature: 656

Protein sequence:

>656_residues
MIFSGAVHSVTENISGEKPVAVRGRKPGGGAKKPEALLPLKELTPVEAEARRTQLKNLIILGKERGFLTYAEINDHLPDE
VLDADQIEGVISMINDMGIQVYDEAPDAETLLMQEAAPAVADEDVVAEAEQALTTVDSEFGRTTDPVRMYMREMGSVDLL
TREGEIEIAKRIEEGLRHMVQAISSCPLTIQQILDMAAQVEKDELRIDELIDGFVEAETEAAAEEETAAGDDETDEDDED
ASAAVAAANLAQLKAEALAQFDYIRKAYKKAQNAHVKYGLGSAQHMKAQAEVTELLMAIRFSVRQVDNLCEQIRSAVEEV
RSHERKIMEFCVNRSGMPRAHFIKAFPGNETNLAWLDREIAAKKAYCSTLSKVQYEVKAHQEALIAMQDRVGLPIKNLKD
INKQMSTGEAKARRAKREMIEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAIT
RSIADQARTIRIPVHMIETINKMNRISRQILQETGIEPDPATLAIKMEMPEDKIRKIMKIAKEPISMETPIGDDEDSHLG
DFIEDASTLSPDDSAIYANLRDATREVLDSLTSREAKVLRMRFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRH
PTRSEKLKSFVGSGEQ

Sequences:

>Translated_656_residues
MIFSGAVHSVTENISGEKPVAVRGRKPGGGAKKPEALLPLKELTPVEAEARRTQLKNLIILGKERGFLTYAEINDHLPDE
VLDADQIEGVISMINDMGIQVYDEAPDAETLLMQEAAPAVADEDVVAEAEQALTTVDSEFGRTTDPVRMYMREMGSVDLL
TREGEIEIAKRIEEGLRHMVQAISSCPLTIQQILDMAAQVEKDELRIDELIDGFVEAETEAAAEEETAAGDDETDEDDED
ASAAVAAANLAQLKAEALAQFDYIRKAYKKAQNAHVKYGLGSAQHMKAQAEVTELLMAIRFSVRQVDNLCEQIRSAVEEV
RSHERKIMEFCVNRSGMPRAHFIKAFPGNETNLAWLDREIAAKKAYCSTLSKVQYEVKAHQEALIAMQDRVGLPIKNLKD
INKQMSTGEAKARRAKREMIEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAIT
RSIADQARTIRIPVHMIETINKMNRISRQILQETGIEPDPATLAIKMEMPEDKIRKIMKIAKEPISMETPIGDDEDSHLG
DFIEDASTLSPDDSAIYANLRDATREVLDSLTSREAKVLRMRFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRH
PTRSEKLKSFVGSGEQ
>Mature_656_residues
MIFSGAVHSVTENISGEKPVAVRGRKPGGGAKKPEALLPLKELTPVEAEARRTQLKNLIILGKERGFLTYAEINDHLPDE
VLDADQIEGVISMINDMGIQVYDEAPDAETLLMQEAAPAVADEDVVAEAEQALTTVDSEFGRTTDPVRMYMREMGSVDLL
TREGEIEIAKRIEEGLRHMVQAISSCPLTIQQILDMAAQVEKDELRIDELIDGFVEAETEAAAEEETAAGDDETDEDDED
ASAAVAAANLAQLKAEALAQFDYIRKAYKKAQNAHVKYGLGSAQHMKAQAEVTELLMAIRFSVRQVDNLCEQIRSAVEEV
RSHERKIMEFCVNRSGMPRAHFIKAFPGNETNLAWLDREIAAKKAYCSTLSKVQYEVKAHQEALIAMQDRVGLPIKNLKD
INKQMSTGEAKARRAKREMIEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAIT
RSIADQARTIRIPVHMIETINKMNRISRQILQETGIEPDPATLAIKMEMPEDKIRKIMKIAKEPISMETPIGDDEDSHLG
DFIEDASTLSPDDSAIYANLRDATREVLDSLTSREAKVLRMRFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRH
PTRSEKLKSFVGSGEQ

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=623, Percent_Identity=56.9823434991974, Blast_Score=671, Evalue=0.0,
Organism=Escherichia coli, GI1789098, Length=234, Percent_Identity=40.1709401709402, Blast_Score=202, Evalue=6e-53,
Organism=Escherichia coli, GI1789871, Length=242, Percent_Identity=26.8595041322314, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007631
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04546 Sigma70_ner; PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 73658; Mature: 73658

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFSGAVHSVTENISGEKPVAVRGRKPGGGAKKPEALLPLKELTPVEAEARRTQLKNLII
CCCCCHHHHHHHCCCCCCCEEEECCCCCCCCCCCHHHCCHHHCCCCHHHHHHHHHHHEEE
LGKERGFLTYAEINDHLPDEVLDADQIEGVISMINDMGIQVYDEAPDAETLLMQEAAPAV
EECCCCEEEEECCCCCCCHHHCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCC
ADEDVVAEAEQALTTVDSEFGRTTDPVRMYMREMGSVDLLTREGEIEIAKRIEEGLRHMV
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHH
QAISSCPLTIQQILDMAAQVEKDELRIDELIDGFVEAETEAAAEEETAAGDDETDEDDED
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
ASAAVAAANLAQLKAEALAQFDYIRKAYKKAQNAHVKYGLGSAQHMKAQAEVTELLMAIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHH
FSVRQVDNLCEQIRSAVEEVRSHERKIMEFCVNRSGMPRAHFIKAFPGNETNLAWLDREI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEECCCCCCCHHHHHHHH
AAKKAYCSTLSKVQYEVKAHQEALIAMQDRVGLPIKNLKDINKQMSTGEAKARRAKREMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
EANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAIT
HHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RSIADQARTIRIPVHMIETINKMNRISRQILQETGIEPDPATLAIKMEMPEDKIRKIMKI
HHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHH
AKEPISMETPIGDDEDSHLGDFIEDASTLSPDDSAIYANLRDATREVLDSLTSREAKVLR
HCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MRFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPTRSEKLKSFVGSGEQ
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MIFSGAVHSVTENISGEKPVAVRGRKPGGGAKKPEALLPLKELTPVEAEARRTQLKNLII
CCCCCHHHHHHHCCCCCCCEEEECCCCCCCCCCCHHHCCHHHCCCCHHHHHHHHHHHEEE
LGKERGFLTYAEINDHLPDEVLDADQIEGVISMINDMGIQVYDEAPDAETLLMQEAAPAV
EECCCCEEEEECCCCCCCHHHCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCC
ADEDVVAEAEQALTTVDSEFGRTTDPVRMYMREMGSVDLLTREGEIEIAKRIEEGLRHMV
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHH
QAISSCPLTIQQILDMAAQVEKDELRIDELIDGFVEAETEAAAEEETAAGDDETDEDDED
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
ASAAVAAANLAQLKAEALAQFDYIRKAYKKAQNAHVKYGLGSAQHMKAQAEVTELLMAIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHH
FSVRQVDNLCEQIRSAVEEVRSHERKIMEFCVNRSGMPRAHFIKAFPGNETNLAWLDREI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEECCCCCCCHHHHHHHH
AAKKAYCSTLSKVQYEVKAHQEALIAMQDRVGLPIKNLKDINKQMSTGEAKARRAKREMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
EANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAIT
HHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RSIADQARTIRIPVHMIETINKMNRISRQILQETGIEPDPATLAIKMEMPEDKIRKIMKI
HHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHH
AKEPISMETPIGDDEDSHLGDFIEDASTLSPDDSAIYANLRDATREVLDSLTSREAKVLR
HCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MRFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPTRSEKLKSFVGSGEQ
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA