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The map label for this gene is pilB [H]

Identifier: 74318388

GI number: 74318388

Start: 2444025

End: 2445740

Strand: Reverse

Name: pilB [H]

Synonym: Tbd_2370

Alternate gene names: 74318388

Gene position: 2445740-2444025 (Counterclockwise)

Preceding gene: 74318389

Following gene: 74318387

Centisome position: 84.05

GC content: 63.11

Gene sequence:

>1716_bases
ATGGCCGCTGTAACGAACACGAACAACCTGACCGGGCTGGCGCGTGCGATGGTCAACCACGGCTGGCTGTCCGAGCAGGA
CGCCGAGGGGGTGTGGAATCGTGCGCACCAGTCCGGCGACTCGTTCGTGACCGAGCTGATCAAGGGGCGGCGCTTCAAGG
CCGACCAGGTCGCCGAATTCGCCGCGGCGTCCTTCGGCTTTCCCTATCTCGACCTCAACGCGATGGACGCCGAGAGCCTG
CCGCAAGGCCTGCTCGACCAGAAGCTGGTGCAGAAGCGGCGGGTGCTCGCGCTCTATCAGCGCGGCAACAAGCTGTACGT
GGCGACCTCCGACCCCACGCACCTGCAAGCGCTCGACGAGGTGAAATTCCAGACCGGGCAGGCCGTCGAACCGGTGGTCG
TCGAAGACGACAAGCTCGGCAAGCTGATCGAAAAGGCAGGCGAGGCCTCGGAAAGCACGATGGCCGTGTTGAACGCCGAA
GACCTCGACCTCGATTTCGCCGACGAAGAGAGCGCCGCCGCAGTCCAGGAGACCGGCGGAATCGAGATCGACGACGCCCC
GGTCGTGCGCTACCTGCAGAAGATCATGCTCGACGCCATCAACATGGGCGCCTCCGACATCCACTTGGAGCCCTACGAGA
AGTTCTACCGCATTCGCTACCGGCGCGACGGCATGCTGTACGAAGTTGCTCAGCCGCCGATGGCGATCAAGGACAAGGTC
GCGTCGCGCATCAAGGTGCTGTCGCGTCTCGACATTTCCGAGAAGCGCGTGCCGCAGGACGGCCGCATGAAGTTGGTGCT
GTCGAAGAACCGCGCGATCGATTTTCGCGTCAGCACCCTGCCGACGCTGTATGGCGAAAAAATCGTGATGCGTATTCTCG
ACCCGAGCAGTGCGCAGCTCGGGATCGAAGCGCTGGGTTACGAGCCCTGGCAGAAGGATCTGCTGATGGACGCAATCCGG
CGCCCCTATGGCATGGTGCTCGTCACCGGGCCGACCGGCTCCGGCAAGACCGTGTCGCTCTATACCTGCCTGAATATTCT
GAACAAGCCCGACGTCAACATTTCGACCGCCGAAGATCCGGCCGAAATCCAGTTGCCCGGAATCAACCAGGTCAACGTCA
ACGATAAGGCTGGGCTGACGTTTTCGGCGGCGCTCAAATCCTTCCTGCGGCAGGACCCCGACGTCATCATGGTCGGCGAG
ATTCGCGATCTCGAAACCGCGGACATCGCGATCAAGGCGGCGCAGACCGGACACATGGTGATGTCGACGCTGCACACCAA
CGATGCCCCCGGCACGCTGACGCGTCTGCTGAACATGGGTGTCGCGCCGTTCAACGTCGCCTCGTCGGTGATTTTGATCA
CCGCCCAGCGCCTCGGTCGACGTCTGTGCAGCTGCAAGCAGCCCGCCGACGTTCCCACGGAAACCCTGCTCGCCGCAGGC
TTCACCGAGGATCAGCTGGACGGGACGTGGCAGCCCTACCGGCCGGTCGGGTGCGAGATATGCGGCGGCAGCGGCTACAA
GGGCCGCGTCGGCATCTATCAGGTCATGCCGATCACCGACGCGATGACGCGGATCATCCTCAAGGGCGGCAACGAATCCG
ACATCGCGGACCAGGCCAAGCGCGAAGGCGTGCTCGATTTGCGCCAGGCGGGTCTGTTGAAGGTAAAAGCCGGGCTCACC
TCGCTGGAAGAGGTCGAGGCCGTTACCAACATTTAA

Upstream 100 bases:

>100_bases
TAGTCGAAAAATCGCCGTTAAGGCGTTTGATTCTGCGTCGACCGCCTTCCCCGCGGGCGATGCCGACCCTGTTTGAGGTG
GATTTGTCGAGAAACGAGCA

Downstream 100 bases:

>100_bases
GGAAATTCTTCGATGGCGACAGCGGCAAAGGCAGTCAAGGACGCGACCTTCCTGTGGGAAGGCATGGACAAGCGCGGCAA
GAAGGTCAAGGGTCAGATCC

Product: type IV pilus assembly protein, PilB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 571; Mature: 570

Protein sequence:

>571_residues
MAAVTNTNNLTGLARAMVNHGWLSEQDAEGVWNRAHQSGDSFVTELIKGRRFKADQVAEFAAASFGFPYLDLNAMDAESL
PQGLLDQKLVQKRRVLALYQRGNKLYVATSDPTHLQALDEVKFQTGQAVEPVVVEDDKLGKLIEKAGEASESTMAVLNAE
DLDLDFADEESAAAVQETGGIEIDDAPVVRYLQKIMLDAINMGASDIHLEPYEKFYRIRYRRDGMLYEVAQPPMAIKDKV
ASRIKVLSRLDISEKRVPQDGRMKLVLSKNRAIDFRVSTLPTLYGEKIVMRILDPSSAQLGIEALGYEPWQKDLLMDAIR
RPYGMVLVTGPTGSGKTVSLYTCLNILNKPDVNISTAEDPAEIQLPGINQVNVNDKAGLTFSAALKSFLRQDPDVIMVGE
IRDLETADIAIKAAQTGHMVMSTLHTNDAPGTLTRLLNMGVAPFNVASSVILITAQRLGRRLCSCKQPADVPTETLLAAG
FTEDQLDGTWQPYRPVGCEICGGSGYKGRVGIYQVMPITDAMTRIILKGGNESDIADQAKREGVLDLRQAGLLKVKAGLT
SLEEVEAVTNI

Sequences:

>Translated_571_residues
MAAVTNTNNLTGLARAMVNHGWLSEQDAEGVWNRAHQSGDSFVTELIKGRRFKADQVAEFAAASFGFPYLDLNAMDAESL
PQGLLDQKLVQKRRVLALYQRGNKLYVATSDPTHLQALDEVKFQTGQAVEPVVVEDDKLGKLIEKAGEASESTMAVLNAE
DLDLDFADEESAAAVQETGGIEIDDAPVVRYLQKIMLDAINMGASDIHLEPYEKFYRIRYRRDGMLYEVAQPPMAIKDKV
ASRIKVLSRLDISEKRVPQDGRMKLVLSKNRAIDFRVSTLPTLYGEKIVMRILDPSSAQLGIEALGYEPWQKDLLMDAIR
RPYGMVLVTGPTGSGKTVSLYTCLNILNKPDVNISTAEDPAEIQLPGINQVNVNDKAGLTFSAALKSFLRQDPDVIMVGE
IRDLETADIAIKAAQTGHMVMSTLHTNDAPGTLTRLLNMGVAPFNVASSVILITAQRLGRRLCSCKQPADVPTETLLAAG
FTEDQLDGTWQPYRPVGCEICGGSGYKGRVGIYQVMPITDAMTRIILKGGNESDIADQAKREGVLDLRQAGLLKVKAGLT
SLEEVEAVTNI
>Mature_570_residues
AAVTNTNNLTGLARAMVNHGWLSEQDAEGVWNRAHQSGDSFVTELIKGRRFKADQVAEFAAASFGFPYLDLNAMDAESLP
QGLLDQKLVQKRRVLALYQRGNKLYVATSDPTHLQALDEVKFQTGQAVEPVVVEDDKLGKLIEKAGEASESTMAVLNAED
LDLDFADEESAAAVQETGGIEIDDAPVVRYLQKIMLDAINMGASDIHLEPYEKFYRIRYRRDGMLYEVAQPPMAIKDKVA
SRIKVLSRLDISEKRVPQDGRMKLVLSKNRAIDFRVSTLPTLYGEKIVMRILDPSSAQLGIEALGYEPWQKDLLMDAIRR
PYGMVLVTGPTGSGKTVSLYTCLNILNKPDVNISTAEDPAEIQLPGINQVNVNDKAGLTFSAALKSFLRQDPDVIMVGEI
RDLETADIAIKAAQTGHMVMSTLHTNDAPGTLTRLLNMGVAPFNVASSVILITAQRLGRRLCSCKQPADVPTETLLAAGF
TEDQLDGTWQPYRPVGCEICGGSGYKGRVGIYQVMPITDAMTRIILKGGNESDIADQAKREGVLDLRQAGLLKVKAGLTS
LEEVEAVTNI

Specific function: Involved in the translocation of the type IV pilin (pilA) [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=484, Percent_Identity=42.3553719008264, Blast_Score=335, Evalue=6e-93,
Organism=Escherichia coli, GI1786296, Length=408, Percent_Identity=41.6666666666667, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI87082188, Length=253, Percent_Identity=30.0395256916996, Blast_Score=105, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 62453; Mature: 62322

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAVTNTNNLTGLARAMVNHGWLSEQDAEGVWNRAHQSGDSFVTELIKGRRFKADQVAEF
CCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHH
AAASFGFPYLDLNAMDAESLPQGLLDQKLVQKRRVLALYQRGNKLYVATSDPTHLQALDE
HHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHH
VKFQTGQAVEPVVVEDDKLGKLIEKAGEASESTMAVLNAEDLDLDFADEESAAAVQETGG
HHHCCCCCCCCEEECCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCC
IEIDDAPVVRYLQKIMLDAINMGASDIHLEPYEKFYRIRYRRDGMLYEVAQPPMAIKDKV
EEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHH
ASRIKVLSRLDISEKRVPQDGRMKLVLSKNRAIDFRVSTLPTLYGEKIVMRILDPSSAQL
HHHHHHHHHCCCHHHCCCCCCCEEEEEECCCEEEEEEHHCCHHHHCHHEEEEECCCCCCC
GIEALGYEPWQKDLLMDAIRRPYGMVLVTGPTGSGKTVSLYTCLNILNKPDVNISTAEDP
CHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCEEEHHHHHHHHCCCCCCEECCCCC
AEIQLPGINQVNVNDKAGLTFSAALKSFLRQDPDVIMVGEIRDLETADIAIKAAQTGHMV
CEEECCCCCEECCCCCCCEEHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEECCCCHHH
MSTLHTNDAPGTLTRLLNMGVAPFNVASSVILITAQRLGRRLCSCKQPADVPTETLLAAG
HHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCC
FTEDQLDGTWQPYRPVGCEICGGSGYKGRVGIYQVMPITDAMTRIILKGGNESDIADQAK
CCHHHCCCCCCCCCCCCCEEECCCCCCCCEEEEEEEEHHHHHHHHHHCCCCCCHHHHHHH
REGVLDLRQAGLLKVKAGLTSLEEVEAVTNI
HCCCHHHHHCCCEEEECCCCHHHHHHHHHCC
>Mature Secondary Structure 
AAVTNTNNLTGLARAMVNHGWLSEQDAEGVWNRAHQSGDSFVTELIKGRRFKADQVAEF
CCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHH
AAASFGFPYLDLNAMDAESLPQGLLDQKLVQKRRVLALYQRGNKLYVATSDPTHLQALDE
HHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHH
VKFQTGQAVEPVVVEDDKLGKLIEKAGEASESTMAVLNAEDLDLDFADEESAAAVQETGG
HHHCCCCCCCCEEECCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCC
IEIDDAPVVRYLQKIMLDAINMGASDIHLEPYEKFYRIRYRRDGMLYEVAQPPMAIKDKV
EEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHH
ASRIKVLSRLDISEKRVPQDGRMKLVLSKNRAIDFRVSTLPTLYGEKIVMRILDPSSAQL
HHHHHHHHHCCCHHHCCCCCCCEEEEEECCCEEEEEEHHCCHHHHCHHEEEEECCCCCCC
GIEALGYEPWQKDLLMDAIRRPYGMVLVTGPTGSGKTVSLYTCLNILNKPDVNISTAEDP
CHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCEEEHHHHHHHHCCCCCCEECCCCC
AEIQLPGINQVNVNDKAGLTFSAALKSFLRQDPDVIMVGEIRDLETADIAIKAAQTGHMV
CEEECCCCCEECCCCCCCEEHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEECCCCHHH
MSTLHTNDAPGTLTRLLNMGVAPFNVASSVILITAQRLGRRLCSCKQPADVPTETLLAAG
HHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCC
FTEDQLDGTWQPYRPVGCEICGGSGYKGRVGIYQVMPITDAMTRIILKGGNESDIADQAK
CCHHHCCCCCCCCCCCCCEEECCCCCCCCEEEEEEEEHHHHHHHHHHCCCCCCHHHHHHH
REGVLDLRQAGLLKVKAGLTSLEEVEAVTNI
HCCCHHHHHCCCEEEECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1971619; 10984043 [H]