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The map label for this gene is tapD [H]

Identifier: 74318386

GI number: 74318386

Start: 2441941

End: 2442792

Strand: Reverse

Name: tapD [H]

Synonym: Tbd_2368

Alternate gene names: 74318386

Gene position: 2442792-2441941 (Counterclockwise)

Preceding gene: 74318387

Following gene: 74318385

Centisome position: 83.95

GC content: 67.37

Gene sequence:

>852_bases
ATGACGCTATTCGTCGCCGAACCGGCCCTCTTCACAGGGCTGGTTTTCCTGCTCGGCCTGCTCGTCGGCAGCTTTCTCAA
CGTCGTCGTCCACCGCCTGCCGAAAATGATGGACGCCGAATGGCAGGCGCAGTGCGCGGAGCTGCGCGGCGAGCCGTCGC
CGGCGGGGCCGCGTTTCAACCTGTGGGCGCCGCGCTCGGCGTGCCCGGCCTGCGGCCACGCGATCACGGCGCTCGAGAAC
GTACCGCTGCTTTCGTGGCTATGGCTTCGCGGACGGTGCGCGTCTTGCAGGACGCCGATCAGCGCGCGCTATCCGCTGGT
CGAACTGCTCGCGGCGCTGCTCTCCGCGGCGGTGGCCTGGAAGTGGGGGCCGACGGTCCAGGCCGCCGGTGCATTGCTGC
TCGTCTGGATGCTCATCGCGCTCGCCTTCATCGATCTTGACACGACGTTGTTGCCGGACAGCCTGACGCTTCCACTGCTC
TGGGCCGGCTTGCTGATCAATCTCGGCGGACATTTCGCTTCCCTTCCTGACGCGGTCGCCGGCGCGGCCGCGGGCTACCT
CGTGCTATGGTCGGTCTACTGGATGTTCAAGCTGGTGACGGGCAAGGAGGGGATGGGCTATGGTGATTTCAAGCTGCTGG
CGGCGATCGGGGCGTGGCTCGGCTGGCAGTTGCTGCCCGTCACGCTTCTGCTCGCGTCGGTCGTCGGGGCGGTGGTCGGC
ATCACGATGATCGTCGCCGTCAAGCACGACCGCCGTGTGCCGATCCCGTTCGGCCCCTACCTCGCGGGCGGGGGACTCGT
TGCCCTGTTCTTCGGTGCGGACCTGACGCAGGCCTATCTCGCGCAGTTCTGA

Upstream 100 bases:

>100_bases
GCCTGATGGAGCCCCTCATCATGGTCGTGTTGGGCACGCTGATCGGTGGGATGGTCATCGCGATGTACCTGCCGATCTTC
AAGATGGGCGCGGTCGTCGG

Downstream 100 bases:

>100_bases
TGTTCACGATCGGGCTGACCGGCGGCATCGGTTCCGGCAAGTCCGCAGCCGCAGAACGGTTTGCCGAGCTCGGCGTGCCG
GTCATCGATACCGACGTGAT

Product: type 4 prepilin peptidase 1

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MTLFVAEPALFTGLVFLLGLLVGSFLNVVVHRLPKMMDAEWQAQCAELRGEPSPAGPRFNLWAPRSACPACGHAITALEN
VPLLSWLWLRGRCASCRTPISARYPLVELLAALLSAAVAWKWGPTVQAAGALLLVWMLIALAFIDLDTTLLPDSLTLPLL
WAGLLINLGGHFASLPDAVAGAAAGYLVLWSVYWMFKLVTGKEGMGYGDFKLLAAIGAWLGWQLLPVTLLLASVVGAVVG
ITMIVAVKHDRRVPIPFGPYLAGGGLVALFFGADLTQAYLAQF

Sequences:

>Translated_283_residues
MTLFVAEPALFTGLVFLLGLLVGSFLNVVVHRLPKMMDAEWQAQCAELRGEPSPAGPRFNLWAPRSACPACGHAITALEN
VPLLSWLWLRGRCASCRTPISARYPLVELLAALLSAAVAWKWGPTVQAAGALLLVWMLIALAFIDLDTTLLPDSLTLPLL
WAGLLINLGGHFASLPDAVAGAAAGYLVLWSVYWMFKLVTGKEGMGYGDFKLLAAIGAWLGWQLLPVTLLLASVVGAVVG
ITMIVAVKHDRRVPIPFGPYLAGGGLVALFFGADLTQAYLAQF
>Mature_282_residues
TLFVAEPALFTGLVFLLGLLVGSFLNVVVHRLPKMMDAEWQAQCAELRGEPSPAGPRFNLWAPRSACPACGHAITALENV
PLLSWLWLRGRCASCRTPISARYPLVELLAALLSAAVAWKWGPTVQAAGALLLVWMLIALAFIDLDTTLLPDSLTLPLLW
AGLLINLGGHFASLPDAVAGAAAGYLVLWSVYWMFKLVTGKEGMGYGDFKLLAAIGAWLGWQLLPVTLLLASVVGAVVGI
TMIVAVKHDRRVPIPFGPYLAGGGLVALFFGADLTQAYLAQF

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue. Processes the tapA pilin precursor during membrane translocation [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=273, Percent_Identity=39.9267399267399, Blast_Score=164, Evalue=4e-42,
Organism=Escherichia coli, GI1789732, Length=143, Percent_Identity=52.4475524475524, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 30277; Mature: 30146

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLFVAEPALFTGLVFLLGLLVGSFLNVVVHRLPKMMDAEWQAQCAELRGEPSPAGPRFN
CEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCC
LWAPRSACPACGHAITALENVPLLSWLWLRGRCASCRTPISARYPLVELLAALLSAAVAW
CCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
KWGPTVQAAGALLLVWMLIALAFIDLDTTLLPDSLTLPLLWAGLLINLGGHFASLPDAVA
CCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHCCCHHHCCHHHH
GAAAGYLVLWSVYWMFKLVTGKEGMGYGDFKLLAAIGAWLGWQLLPVTLLLASVVGAVVG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITMIVAVKHDRRVPIPFGPYLAGGGLVALFFGADLTQAYLAQF
HHHHHHCCCCCCCCCCCCCCHHCCCHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure 
TLFVAEPALFTGLVFLLGLLVGSFLNVVVHRLPKMMDAEWQAQCAELRGEPSPAGPRFN
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCC
LWAPRSACPACGHAITALENVPLLSWLWLRGRCASCRTPISARYPLVELLAALLSAAVAW
CCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
KWGPTVQAAGALLLVWMLIALAFIDLDTTLLPDSLTLPLLWAGLLINLGGHFASLPDAVA
CCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHCCCHHHCCHHHH
GAAAGYLVLWSVYWMFKLVTGKEGMGYGDFKLLAAIGAWLGWQLLPVTLLLASVVGAVVG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITMIVAVKHDRRVPIPFGPYLAGGGLVALFFGADLTQAYLAQF
HHHHHHCCCCCCCCCCCCCCHHCCCHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA