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The map label for this gene is cca [H]

Identifier: 74318378

GI number: 74318378

Start: 2433239

End: 2434867

Strand: Reverse

Name: cca [H]

Synonym: Tbd_2360

Alternate gene names: 74318378

Gene position: 2434867-2433239 (Counterclockwise)

Preceding gene: 74318379

Following gene: 74318377

Centisome position: 83.68

GC content: 68.14

Gene sequence:

>1629_bases
ATGAAGACCTACGTCGTCGGCGGCGCGGTGCGCGACCGCTTGCTGGGCCTGCAGGTCAGCGACCGCGACCACGTCGTCGT
CGGCGCCACCCCCGACGAGATGCTGGCGGCGGGCTTTCGGCCGGTCGGCAAGGACTTCCCTGTCTTCCTTCATCCGCACA
CCCACGAGGAGTATGCGCTCGCGCGCACCGAGCGCAAATCCGGCCGCGGCTACAAGGGCTTCGTCGTGCATGCGGCGCCC
GAGGTCACCCTCGAAGAGGACTTGGCGCGGCGCGACCTCACGATCAACGCGATCGCCGAGGACGAAAGTGGGACCCTGAT
CGACCCCTACGATGGCCAGGCGGACCTCGCGGCGAAGACCTTCCGCCACGTCAGCGAGGCCTTCGCCGAGGATCCGGTCC
GCATCCTGCGCGTCGCGCGCTTCGCCGCGCGCTTCACCGAATTCACCGTCGCCCCGGAAACCAATGCCTTGATGCGGCGC
ATGGTCGACAGCGGCGAAGTCGACGCACTGGTCGCCGAACGCGTCTGGCAGGAGATCGCCCGCGGCCTCATGGAGACGCA
GCCGTCGCGCATGTTCGCGGTGCTGCGCGACTGCGGCGCGCTCGCCCGGATGCTTCCCGAAGTCGACCGCCTGTTCGGCG
TGCCGCAGCCGCCCGAGCATCATCCCGAGGTCGACACGGGGATCCACGTCATGCTCGTGATCGACTGGGCGGCGCGGCAG
GGCGCGAACCTGGCCGTGCGCTTCGCGGCCCTGACCCACGACCTCGGCAAGGGCGAGACCTCGCCCGAATTGTGGCCCAG
GCACCACGGCCACGAAGGCGCGAGCGTGAGACTCGTGCGCGCGCTGAGCGAGCGCCTGCGCGCACCCGCCGAGTGCCGCG
AACTCGCGGTCGCGGTAGCGCGCGATCATGGCAACGTACATCGTGCGCTCGAATTGCGACCGCGCACGATCGTCGAACTG
CTCGAACGCGTCGATGCCTTCCGCCGCCCCGAACGCTTCGAACATTTTCTGGAAGCCTGTGAATGTGATTTCCGCGGGCG
GCCCGGCTACGCGGACAAGACTTACCCGCCGCCGCAATACCTGCGGCAGGCGTTGCACACGGCGCAGCAGATCGACGCGG
CTGCGGTCGCGCGCAGCGTTGAGTCGGTGAGAATTCGTGAAGCGATACTCGCGGCGCGCGTGGAGGCAGTGAACCGGTGG
CGGCGCAGTCGCGCGAGCCGCTGGGAACAGTTCTCGCATGAAGCCGACATCGGTGTGCGCGGCATCGGCCCCGATCTGGC
GGCCGCCTTCGAGCAGGTGGCCGTCGCGATGACGGCGGTGATTACCGACCCCGCGCGGGTCGCGACGGAAACCTGCGTCG
AGATACGCTGCGACGCCGCTGACGACGAGCTTCTGCTCGTCGACTGGCTCAACGCGCTGATCTACGAAATGGCGGTTCGC
CATATGCTCTTCGGCCGTTTCGAGGTGCACCTCGACCGCCGCCGTTTGTACGCGAAAGCCTGGGGCGAGGCCGTCGACGC
GCCCAGGCATCAACCCGTCGTCGAGATCAAGGGCGCGACCTATACTGGCCTGAAGGTAGGGAGAGACGAAACCGGGCAAT
GGCAGGCCCAGTGCATCGTGGACGTGTGA

Upstream 100 bases:

>100_bases
CTCGAAGCGATCGCGCCCGAATACCTGCGCGCCGACACGCCGCGCGCGCGCTACGAGGACTACCGCGAGACCGCCCGCCG
CTAGGCGCCCCGCGCATGAC

Downstream 100 bases:

>100_bases
CATGGATCTTGCGCGCTTGCAGCAGGCATCGGCGGTCGAGTGGCGGATCGGCCCGCACGGGCGCATGCGCGTGCCGGCCG
TCATCTTCGCCGACGAAGAC

Product: metal dependent phosphohydrolase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 542; Mature: 542

Protein sequence:

>542_residues
MKTYVVGGAVRDRLLGLQVSDRDHVVVGATPDEMLAAGFRPVGKDFPVFLHPHTHEEYALARTERKSGRGYKGFVVHAAP
EVTLEEDLARRDLTINAIAEDESGTLIDPYDGQADLAAKTFRHVSEAFAEDPVRILRVARFAARFTEFTVAPETNALMRR
MVDSGEVDALVAERVWQEIARGLMETQPSRMFAVLRDCGALARMLPEVDRLFGVPQPPEHHPEVDTGIHVMLVIDWAARQ
GANLAVRFAALTHDLGKGETSPELWPRHHGHEGASVRLVRALSERLRAPAECRELAVAVARDHGNVHRALELRPRTIVEL
LERVDAFRRPERFEHFLEACECDFRGRPGYADKTYPPPQYLRQALHTAQQIDAAAVARSVESVRIREAILAARVEAVNRW
RRSRASRWEQFSHEADIGVRGIGPDLAAAFEQVAVAMTAVITDPARVATETCVEIRCDAADDELLLVDWLNALIYEMAVR
HMLFGRFEVHLDRRRLYAKAWGEAVDAPRHQPVVEIKGATYTGLKVGRDETGQWQAQCIVDV

Sequences:

>Translated_542_residues
MKTYVVGGAVRDRLLGLQVSDRDHVVVGATPDEMLAAGFRPVGKDFPVFLHPHTHEEYALARTERKSGRGYKGFVVHAAP
EVTLEEDLARRDLTINAIAEDESGTLIDPYDGQADLAAKTFRHVSEAFAEDPVRILRVARFAARFTEFTVAPETNALMRR
MVDSGEVDALVAERVWQEIARGLMETQPSRMFAVLRDCGALARMLPEVDRLFGVPQPPEHHPEVDTGIHVMLVIDWAARQ
GANLAVRFAALTHDLGKGETSPELWPRHHGHEGASVRLVRALSERLRAPAECRELAVAVARDHGNVHRALELRPRTIVEL
LERVDAFRRPERFEHFLEACECDFRGRPGYADKTYPPPQYLRQALHTAQQIDAAAVARSVESVRIREAILAARVEAVNRW
RRSRASRWEQFSHEADIGVRGIGPDLAAAFEQVAVAMTAVITDPARVATETCVEIRCDAADDELLLVDWLNALIYEMAVR
HMLFGRFEVHLDRRRLYAKAWGEAVDAPRHQPVVEIKGATYTGLKVGRDETGQWQAQCIVDV
>Mature_542_residues
MKTYVVGGAVRDRLLGLQVSDRDHVVVGATPDEMLAAGFRPVGKDFPVFLHPHTHEEYALARTERKSGRGYKGFVVHAAP
EVTLEEDLARRDLTINAIAEDESGTLIDPYDGQADLAAKTFRHVSEAFAEDPVRILRVARFAARFTEFTVAPETNALMRR
MVDSGEVDALVAERVWQEIARGLMETQPSRMFAVLRDCGALARMLPEVDRLFGVPQPPEHHPEVDTGIHVMLVIDWAARQ
GANLAVRFAALTHDLGKGETSPELWPRHHGHEGASVRLVRALSERLRAPAECRELAVAVARDHGNVHRALELRPRTIVEL
LERVDAFRRPERFEHFLEACECDFRGRPGYADKTYPPPQYLRQALHTAQQIDAAAVARSVESVRIREAILAARVEAVNRW
RRSRASRWEQFSHEADIGVRGIGPDLAAAFEQVAVAMTAVITDPARVATETCVEIRCDAADDELLLVDWLNALIYEMAVR
HMLFGRFEVHLDRRRLYAKAWGEAVDAPRHQPVVEIKGATYTGLKVGRDETGQWQAQCIVDV

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=208, Percent_Identity=29.3269230769231, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1789436, Length=412, Percent_Identity=55.0970873786408, Blast_Score=449, Evalue=1e-127,
Organism=Escherichia coli, GI87081691, Length=227, Percent_Identity=29.5154185022026, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 60720; Mature: 60720

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTYVVGGAVRDRLLGLQVSDRDHVVVGATPDEMLAAGFRPVGKDFPVFLHPHTHEEYAL
CCEEEECHHHHHHHHCCEECCCCCEEEECCHHHHHHHCCCCCCCCCCEEECCCCCCHHHH
ARTERKSGRGYKGFVVHAAPEVTLEEDLARRDLTINAIAEDESGTLIDPYDGQADLAAKT
HHHHHHCCCCCCEEEEECCCCCCHHHHHHHCCCEEEEEEECCCCCEECCCCCCHHHHHHH
FRHVSEAFAEDPVRILRVARFAARFTEFTVAPETNALMRRMVDSGEVDALVAERVWQEIA
HHHHHHHHHHCHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
RGLMETQPSRMFAVLRDCGALARMLPEVDRLFGVPQPPEHHPEVDTGIHVMLVIDWAARQ
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCC
GANLAVRFAALTHDLGKGETSPELWPRHHGHEGASVRLVRALSERLRAPAECRELAVAVA
CCCEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
RDHGNVHRALELRPRTIVELLERVDAFRRPERFEHFLEACECDFRGRPGYADKTYPPPQY
HCCCCHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHH
LRQALHTAQQIDAAAVARSVESVRIREAILAARVEAVNRWRRSRASRWEQFSHEADIGVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GIGPDLAAAFEQVAVAMTAVITDPARVATETCVEIRCDAADDELLLVDWLNALIYEMAVR
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEECCCCCCEEHHHHHHHHHHHHHHH
HMLFGRFEVHLDRRRLYAKAWGEAVDAPRHQPVVEIKGATYTGLKVGRDETGQWQAQCIV
HHHHHHHHEEHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEECEEECCCCCCCEEEEEEE
DV
CC
>Mature Secondary Structure
MKTYVVGGAVRDRLLGLQVSDRDHVVVGATPDEMLAAGFRPVGKDFPVFLHPHTHEEYAL
CCEEEECHHHHHHHHCCEECCCCCEEEECCHHHHHHHCCCCCCCCCCEEECCCCCCHHHH
ARTERKSGRGYKGFVVHAAPEVTLEEDLARRDLTINAIAEDESGTLIDPYDGQADLAAKT
HHHHHHCCCCCCEEEEECCCCCCHHHHHHHCCCEEEEEEECCCCCEECCCCCCHHHHHHH
FRHVSEAFAEDPVRILRVARFAARFTEFTVAPETNALMRRMVDSGEVDALVAERVWQEIA
HHHHHHHHHHCHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
RGLMETQPSRMFAVLRDCGALARMLPEVDRLFGVPQPPEHHPEVDTGIHVMLVIDWAARQ
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCC
GANLAVRFAALTHDLGKGETSPELWPRHHGHEGASVRLVRALSERLRAPAECRELAVAVA
CCCEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
RDHGNVHRALELRPRTIVELLERVDAFRRPERFEHFLEACECDFRGRPGYADKTYPPPQY
HCCCCHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHH
LRQALHTAQQIDAAAVARSVESVRIREAILAARVEAVNRWRRSRASRWEQFSHEADIGVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GIGPDLAAAFEQVAVAMTAVITDPARVATETCVEIRCDAADDELLLVDWLNALIYEMAVR
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEECCCCCCEEHHHHHHHHHHHHHHH
HMLFGRFEVHLDRRRLYAKAWGEAVDAPRHQPVVEIKGATYTGLKVGRDETGQWQAQCIV
HHHHHHHHEEHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEECEEECCCCCCCEEEEEEE
DV
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA