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The map label for this gene is ksgA

Identifier: 74318358

GI number: 74318358

Start: 2415310

End: 2416077

Strand: Direct

Name: ksgA

Synonym: Tbd_2340

Alternate gene names: 74318358

Gene position: 2415310-2416077 (Clockwise)

Preceding gene: 74318357

Following gene: 74318359

Centisome position: 83.01

GC content: 66.02

Gene sequence:

>768_bases
ATGCACACACCGCGCAAACGCTTCGGCCAGAATTTCCTGATCGATGACGGCATCGTCCATGCGATCGTCAACGCGATTCA
CCCACAGGCCGGCGAGACCGTGGTCGAAATCGGCCCCGGGCTCGGCGCCCTGACGCGCCCGCTGCTCGAACGCCTGCCCC
ACCTCCATGCCGTCGAACTCGACCGCGACATCATCGCCCGCCTGCGCAGGGCCTGGCCACCGGAACGGCTCACACTCCAC
GCCGGCGACGCGCTCAAGTTCGACTTCGGCAGCCTCGGCGACGACCTGCGCATCGTCGGCAACCTGCCCTACAACATTTC
GACGCCGCTGCTGTTTCATTTGCTCGAATTCGCTCCACGTATCCGCGACATGCATTTCATGCTGCAGAAGGAGGTCGTCG
AGCGCATGGTGGCCTCACCGGCAACGGCCGACTACGGGCGCCTTTCGATCATGCTGCAGCGCCGATTCCACATGGAATGG
CTGCTCGACGTGCCGCCCACGGCCTTCGATCCGCCGCCGAAAGTCGAGTCGGCGGTGGTGCGCCTGATTCCCAAGTCGAC
GGCCGAGGTCCCGTCCGTCGACGAGGCGCTTTTCGCGCGCGTCGTGGCCGCCGCCTTCGCGCAGCGGCGCAAGACGCTCA
GAAACACGCTTTCCGCACTGATGCGCCCCGAGGATTTCGTAGCGCTCGGAATCGATCCGGGCCTGCGCGCGGAGGCGCTG
CACGTCGCGGACTACGAGGCGATCACGGCATACCTGGCCACACGCTGA

Upstream 100 bases:

>100_bases
GGCAGCCTCGTCGCCGCGATCGCAATGGCGACCGAGATGGCCACCCGGGACGCGGTGGCTCCCTGAGCCGCCCCAACCCT
CGGTCTCCGCCTCACCAGCC

Downstream 100 bases:

>100_bases
AAAGCGCGCTCTACGCAAAAACCCTGAGACCGTCACGCTATTGCGGGCGCGCACGTCCGTTATGATTGAAGGGTTCGTGC
TTGCACGCCGAAGCGGGCAA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MHTPRKRFGQNFLIDDGIVHAIVNAIHPQAGETVVEIGPGLGALTRPLLERLPHLHAVELDRDIIARLRRAWPPERLTLH
AGDALKFDFGSLGDDLRIVGNLPYNISTPLLFHLLEFAPRIRDMHFMLQKEVVERMVASPATADYGRLSIMLQRRFHMEW
LLDVPPTAFDPPPKVESAVVRLIPKSTAEVPSVDEALFARVVAAAFAQRRKTLRNTLSALMRPEDFVALGIDPGLRAEAL
HVADYEAITAYLATR

Sequences:

>Translated_255_residues
MHTPRKRFGQNFLIDDGIVHAIVNAIHPQAGETVVEIGPGLGALTRPLLERLPHLHAVELDRDIIARLRRAWPPERLTLH
AGDALKFDFGSLGDDLRIVGNLPYNISTPLLFHLLEFAPRIRDMHFMLQKEVVERMVASPATADYGRLSIMLQRRFHMEW
LLDVPPTAFDPPPKVESAVVRLIPKSTAEVPSVDEALFARVVAAAFAQRRKTLRNTLSALMRPEDFVALGIDPGLRAEAL
HVADYEAITAYLATR
>Mature_255_residues
MHTPRKRFGQNFLIDDGIVHAIVNAIHPQAGETVVEIGPGLGALTRPLLERLPHLHAVELDRDIIARLRRAWPPERLTLH
AGDALKFDFGSLGDDLRIVGNLPYNISTPLLFHLLEFAPRIRDMHFMLQKEVVERMVASPATADYGRLSIMLQRRFHMEW
LLDVPPTAFDPPPKVESAVVRLIPKSTAEVPSVDEALFARVVAAAFAQRRKTLRNTLSALMRPEDFVALGIDPGLRAEAL
HVADYEAITAYLATR

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=209, Percent_Identity=36.3636363636364, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI156415992, Length=277, Percent_Identity=29.2418772563177, Blast_Score=94, Evalue=9e-20,
Organism=Escherichia coli, GI1786236, Length=257, Percent_Identity=52.9182879377432, Blast_Score=268, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI25146882, Length=209, Percent_Identity=38.2775119617225, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI25141369, Length=256, Percent_Identity=28.125, Blast_Score=88, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6324989, Length=210, Percent_Identity=32.3809523809524, Blast_Score=93, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21358017, Length=217, Percent_Identity=37.3271889400922, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21357273, Length=244, Percent_Identity=32.3770491803279, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_THIDA (Q3SGF7)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_316098.1
- ProteinModelPortal:   Q3SGF7
- SMR:   Q3SGF7
- STRING:   Q3SGF7
- GeneID:   3673468
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_2340
- NMPDR:   fig|292415.3.peg.2413
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   TPIIMKL
- PhylomeDB:   Q3SGF7
- ProtClustDB:   PRK00274
- BioCyc:   TDEN292415:TBD_2340-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 28535; Mature: 28535

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 11-11 BINDING 13-13 BINDING 38-38 BINDING 59-59 BINDING 83-83 BINDING 101-101

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTPRKRFGQNFLIDDGIVHAIVNAIHPQAGETVVEIGPGLGALTRPLLERLPHLHAVEL
CCCCHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCEEHHC
DRDIIARLRRAWPPERLTLHAGDALKFDFGSLGDDLRIVGNLPYNISTPLLFHLLEFAPR
CHHHHHHHHHCCCCCCEEEECCCEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHH
IRDMHFMLQKEVVERMVASPATADYGRLSIMLQRRFHMEWLLDVPPTAFDPPPKVESAVV
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
RLIPKSTAEVPSVDEALFARVVAAAFAQRRKTLRNTLSALMRPEDFVALGIDPGLRAEAL
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHE
HVADYEAITAYLATR
EHHHHHHHHHHHHCC
>Mature Secondary Structure
MHTPRKRFGQNFLIDDGIVHAIVNAIHPQAGETVVEIGPGLGALTRPLLERLPHLHAVEL
CCCCHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCEEHHC
DRDIIARLRRAWPPERLTLHAGDALKFDFGSLGDDLRIVGNLPYNISTPLLFHLLEFAPR
CHHHHHHHHHCCCCCCEEEECCCEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHH
IRDMHFMLQKEVVERMVASPATADYGRLSIMLQRRFHMEWLLDVPPTAFDPPPKVESAVV
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
RLIPKSTAEVPSVDEALFARVVAAAFAQRRKTLRNTLSALMRPEDFVALGIDPGLRAEAL
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHE
HVADYEAITAYLATR
EHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA