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The map label for this gene is ruvC

Identifier: 74318232

GI number: 74318232

Start: 2292927

End: 2293442

Strand: Reverse

Name: ruvC

Synonym: Tbd_2214

Alternate gene names: 74318232

Gene position: 2293442-2292927 (Counterclockwise)

Preceding gene: 74318233

Following gene: 74318231

Centisome position: 78.82

GC content: 69.77

Gene sequence:

>516_bases
TTGCGCATCCTCGGCATCGATCCCGGCCTCAGGCTCACCGGCTTCGGCCTGATCGAGCAGACCGGGCAGAAGCTCGCCTA
CGTCGCGAGCGGCGTGGTCAAGAGCGGCGAGGGCAGCCTGCCGGCGCGCCTGGGCATACTATTCGGCGGGATCAACGAGG
TCATCGCGACCTATCGGCCCGACACCTGCGCCGTCGAGATCGTTTTCGTCAACGTCAACCCGCAATCGACGCTGCTGCTC
GGGCAGGCCCGCGGCGCCGCGATCTGCGCGGCGGTCTCGAACGACCTCACGGTCGCCGAATACACGGCGCTGCAGATCAA
GCAGGCCGTCGTCGGCCACGGCAAGGCGGCGAAGACGCAGGTCCAAGAAATGGTCAAGCGGCTGCTCGCCCTGCCGATCG
CGCCGACGGCCGACGCCGCCGACGCGCTGGCCTGCGCGATCACCCATGCCCACGGCAGCCGCCTCGGCGCGCAGTCGACC
GCGGGCTATCGTCTCAAGGGCGGGCGGCTGGTCTGA

Upstream 100 bases:

>100_bases
CGACGACGCCGCGAAGATGCAGAAGATCCTGGACGCGCTCGAAAGCCTCGACGACGTGCAGGAGGTCTACACCAACGCCG
TGTTGCCGGACTAGGCGTCT

Downstream 100 bases:

>100_bases
GCCGCCGCTTGTTTTCCGCACCCTTTTCGCCCTCGCGCCGGTTCGCTTCCGGCGGTGCGCGCCGCGGTCCCGTGCAGTCG
CCGCGTATTGCGGATATCCT

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 171; Mature: 171

Protein sequence:

>171_residues
MRILGIDPGLRLTGFGLIEQTGQKLAYVASGVVKSGEGSLPARLGILFGGINEVIATYRPDTCAVEIVFVNVNPQSTLLL
GQARGAAICAAVSNDLTVAEYTALQIKQAVVGHGKAAKTQVQEMVKRLLALPIAPTADAADALACAITHAHGSRLGAQST
AGYRLKGGRLV

Sequences:

>Translated_171_residues
MRILGIDPGLRLTGFGLIEQTGQKLAYVASGVVKSGEGSLPARLGILFGGINEVIATYRPDTCAVEIVFVNVNPQSTLLL
GQARGAAICAAVSNDLTVAEYTALQIKQAVVGHGKAAKTQVQEMVKRLLALPIAPTADAADALACAITHAHGSRLGAQST
AGYRLKGGRLV
>Mature_171_residues
MRILGIDPGLRLTGFGLIEQTGQKLAYVASGVVKSGEGSLPARLGILFGGINEVIATYRPDTCAVEIVFVNVNPQSTLLL
GQARGAAICAAVSNDLTVAEYTALQIKQAVVGHGKAAKTQVQEMVKRLLALPIAPTADAADALACAITHAHGSRLGAQST
AGYRLKGGRLV

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=169, Percent_Identity=52.6627218934911, Blast_Score=150, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_THIDA (Q3SGT0)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315972.1
- ProteinModelPortal:   Q3SGT0
- SMR:   Q3SGT0
- STRING:   Q3SGT0
- GeneID:   3671605
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_2214
- NMPDR:   fig|292415.3.peg.2145
- eggNOG:   COG0817
- HOGENOM:   HBG437552
- OMA:   RTGWGVI
- PhylomeDB:   Q3SGT0
- ProtClustDB:   PRK00039
- BioCyc:   TDEN292415:TBD_2214-MONOMER
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 17647; Mature: 17647

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS01321 RUVC; PS00659 GLYCOSYL_HYDROL_F5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILGIDPGLRLTGFGLIEQTGQKLAYVASGVVKSGEGSLPARLGILFGGINEVIATYRP
CEEEECCCCCEEEECHHHHHHCHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHCCC
DTCAVEIVFVNVNPQSTLLLGQARGAAICAAVSNDLTVAEYTALQIKQAVVGHGKAAKTQ
CCEEEEEEEEEECCCCEEEEECCCCEEEEEEECCCCEEHHHHHHHHHHHHHCCCCHHHHH
VQEMVKRLLALPIAPTADAADALACAITHAHGSRLGAQSTAGYRLKGGRLV
HHHHHHHHHHCCCCCCCCHHHHHHHEEECCCCCCCCCCCCCCEEECCCCCC
>Mature Secondary Structure
MRILGIDPGLRLTGFGLIEQTGQKLAYVASGVVKSGEGSLPARLGILFGGINEVIATYRP
CEEEECCCCCEEEECHHHHHHCHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHCCC
DTCAVEIVFVNVNPQSTLLLGQARGAAICAAVSNDLTVAEYTALQIKQAVVGHGKAAKTQ
CCEEEEEEEEEECCCCEEEEECCCCEEEEEEECCCCEEHHHHHHHHHHHHHCCCCHHHHH
VQEMVKRLLALPIAPTADAADALACAITHAHGSRLGAQSTAGYRLKGGRLV
HHHHHHHHHHCCCCCCCCHHHHHHHEEECCCCCCCCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA