Click here to switch to the map view.

The map label for this gene is tolB

Identifier: 74318224

GI number: 74318224

Start: 2285905

End: 2287188

Strand: Reverse

Name: tolB

Synonym: Tbd_2206

Alternate gene names: 74318224

Gene position: 2287188-2285905 (Counterclockwise)

Preceding gene: 74318225

Following gene: 74318223

Centisome position: 78.6

GC content: 66.9

Gene sequence:

>1284_bases
ATGCCTGTATCCCTGTTGCGTGCCCTCCTGGTTTTTTCCCTGCTGTGCCTGGGTCTGAGCGCCACGCGCGCTGCTCACGC
CGCGATGGAGATCGAAGTCGTCGGCGGTGCCGCGAACAAGATCGCGATCGCGATGGTGCCCTTCCAGACCGCTGCGGGTC
AGCCTTCGCCGCCGCTCACGCAGATCGTCGCCGACGATCTCGCGCGCAGCGGACAGTTCAGCATGACCGACGTCACCGGT
GCGGCGCAGCCGGTCGAGCCGTCCCAGGTGGACTATGCGGCGTGGCGGACCAAGGGCGCGGAAGCGATGGTCATCGGGCA
GGTCGTCGCGCTGGGCGGCGGGCGTTTCGAAGTACGCTTCAGATTGCTCGACGTCGTCAAGGGCACGCAACTCGCAGGCT
ATAGCTACAAGATCACGGCGGCGCAGTGGCGCGCCACGGGCCATCGAATCGCCGATGTCGTGTACGAAAAATTGACCGGC
ATCCCCGGCGCGTTCAGCAGCCGCATCGCCTACGTGCAGAAGCAGGGCAAGCGTTACGAACTGCGCGTCGCCGACGCCGA
CGGGCAGAATCCGCGCACGATCGTGCGTTCGCTCGAGCCCTTGATTTCGCCGATGTTTTCGCCCGACGGCACGCGTCTCG
CCTACGTGTCCTTCGAGGACAAGAAGCCCGTGGTGTACGTGCAGTCGCTGCAGCAGGGCGGACGGCGCAAGGTCGCCGCG
TTCAAGGGTTCCAATTCGGCGCCGGCCTGGTCGCCCGACGGCCGGCAGCTCGCGGTCGTGCTGACCCGCGACGGGGCGTC
GCAGATCTACCTGATCAACGTCGACGGCAGCGGCCTCACACGTCTCATGCGAAGCGGCGACATCGACACCGAACCGGTAT
TCTCGCCGGACGGGCAGACGCTTTATTTCACGTCCGATCGCGGGGGCAGTCCGCAGATTTACCGCGTCGCGAAAAGCGGC
GGCGACGCGAAGCGGGTGAGCTTCGAGGGCAGTTACAATGTTTCGCCGACGATCAGCCCGGACGGACGCTATCTTGCATA
CATCAGCCGCGACGGCGGGCGCTTTCGCGTCGTGCTGCAGGAACTTGCCTCCGGACAGACGCGTGTGCTGACCGAAACGA
CGCGCGACGAGGCGCCGAGCTTCGCGCCGAACGGGCAGGCCGTGCTGTACGCGACGGTGCAGGGCGGACGCGGCGTGCTC
GGAACCGTCACCCTCGACGGCAAAACGCGCGCCCGCCTCTCGGAATCCGGCGTCGACGCGCGCGAGCCTGCGTGGGGGCC
GTAG

Upstream 100 bases:

>100_bases
AAGGAGTGAGCTGTAGCGCCCCGTGGGGCGCCACGCCGCCGCCGGCCTGGCCTGAAGGCCTGTGCTAAACTCACCCGCCA
ACCCTGTGCTAGCGAACAAG

Downstream 100 bases:

>100_bases
GTGTTGGCTTGAGTTTGGGAGGGTTGGGCAGCCATCCGCTTGCGGAAGCTGAACTGCCCATACATTGTTTTCATTAAAAG
GAGAGATCCATGAAGAAGAT

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MPVSLLRALLVFSLLCLGLSATRAAHAAMEIEVVGGAANKIAIAMVPFQTAAGQPSPPLTQIVADDLARSGQFSMTDVTG
AAQPVEPSQVDYAAWRTKGAEAMVIGQVVALGGGRFEVRFRLLDVVKGTQLAGYSYKITAAQWRATGHRIADVVYEKLTG
IPGAFSSRIAYVQKQGKRYELRVADADGQNPRTIVRSLEPLISPMFSPDGTRLAYVSFEDKKPVVYVQSLQQGGRRKVAA
FKGSNSAPAWSPDGRQLAVVLTRDGASQIYLINVDGSGLTRLMRSGDIDTEPVFSPDGQTLYFTSDRGGSPQIYRVAKSG
GDAKRVSFEGSYNVSPTISPDGRYLAYISRDGGRFRVVLQELASGQTRVLTETTRDEAPSFAPNGQAVLYATVQGGRGVL
GTVTLDGKTRARLSESGVDAREPAWGP

Sequences:

>Translated_427_residues
MPVSLLRALLVFSLLCLGLSATRAAHAAMEIEVVGGAANKIAIAMVPFQTAAGQPSPPLTQIVADDLARSGQFSMTDVTG
AAQPVEPSQVDYAAWRTKGAEAMVIGQVVALGGGRFEVRFRLLDVVKGTQLAGYSYKITAAQWRATGHRIADVVYEKLTG
IPGAFSSRIAYVQKQGKRYELRVADADGQNPRTIVRSLEPLISPMFSPDGTRLAYVSFEDKKPVVYVQSLQQGGRRKVAA
FKGSNSAPAWSPDGRQLAVVLTRDGASQIYLINVDGSGLTRLMRSGDIDTEPVFSPDGQTLYFTSDRGGSPQIYRVAKSG
GDAKRVSFEGSYNVSPTISPDGRYLAYISRDGGRFRVVLQELASGQTRVLTETTRDEAPSFAPNGQAVLYATVQGGRGVL
GTVTLDGKTRARLSESGVDAREPAWGP
>Mature_426_residues
PVSLLRALLVFSLLCLGLSATRAAHAAMEIEVVGGAANKIAIAMVPFQTAAGQPSPPLTQIVADDLARSGQFSMTDVTGA
AQPVEPSQVDYAAWRTKGAEAMVIGQVVALGGGRFEVRFRLLDVVKGTQLAGYSYKITAAQWRATGHRIADVVYEKLTGI
PGAFSSRIAYVQKQGKRYELRVADADGQNPRTIVRSLEPLISPMFSPDGTRLAYVSFEDKKPVVYVQSLQQGGRRKVAAF
KGSNSAPAWSPDGRQLAVVLTRDGASQIYLINVDGSGLTRLMRSGDIDTEPVFSPDGQTLYFTSDRGGSPQIYRVAKSGG
DAKRVSFEGSYNVSPTISPDGRYLAYISRDGGRFRVVLQELASGQTRVLTETTRDEAPSFAPNGQAVLYATVQGGRGVLG
TVTLDGKTRARLSESGVDAREPAWGP

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=430, Percent_Identity=40.6976744186046, Blast_Score=300, Evalue=8e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_THIDA (Q3SGT4)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315964.1
- ProteinModelPortal:   Q3SGT4
- SMR:   Q3SGT4
- STRING:   Q3SGT4
- GeneID:   3671597
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_2206
- NMPDR:   fig|292415.3.peg.2093
- eggNOG:   COG0823
- HOGENOM:   HBG644919
- OMA:   RVADYDG
- PhylomeDB:   Q3SGT4
- ProtClustDB:   PRK02889
- BioCyc:   TDEN292415:TBD_2206-MONOMER
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943
- Gene3D:   G3DSA:3.40.50.10070
- Gene3D:   G3DSA:2.130.10.10
- TIGRFAMs:   TIGR02800

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 45652; Mature: 45521

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVSLLRALLVFSLLCLGLSATRAAHAAMEIEVVGGAANKIAIAMVPFQTAAGQPSPPLT
CCHHHHHHHHHHHHHHHCCHHHHHHHEEEEEEEECCCCCEEEEEEEECHHCCCCCCCCHH
QIVADDLARSGQFSMTDVTGAAQPVEPSQVDYAAWRTKGAEAMVIGQVVALGGGRFEVRF
HHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHCCCCCEEEEEEEEEECCCEEEEEE
RLLDVVKGTQLAGYSYKITAAQWRATGHRIADVVYEKLTGIPGAFSSRIAYVQKQGKRYE
EEEEHHCCCEECCEEEEEEEHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEE
LRVADADGQNPRTIVRSLEPLISPMFSPDGTRLAYVSFEDKKPVVYVQSLQQGGRRKVAA
EEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEHHHCCCCEEEEE
FKGSNSAPAWSPDGRQLAVVLTRDGASQIYLINVDGSGLTRLMRSGDIDTEPVFSPDGQT
EECCCCCCCCCCCCCEEEEEEEECCCCEEEEEEECCHHHHHHHHCCCCCCCCCCCCCCCE
LYFTSDRGGSPQIYRVAKSGGDAKRVSFEGSYNVSPTISPDGRYLAYISRDGGRFRVVLQ
EEEECCCCCCCEEEEEECCCCCEEEEEECCCCCCCCEECCCCCEEEEEECCCCEEEEEHH
ELASGQTRVLTETTRDEAPSFAPNGQAVLYATVQGGRGVLGTVTLDGKTRARLSESGVDA
HHHCCCEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCHHHHHHCCCCC
REPAWGP
CCCCCCC
>Mature Secondary Structure 
PVSLLRALLVFSLLCLGLSATRAAHAAMEIEVVGGAANKIAIAMVPFQTAAGQPSPPLT
CHHHHHHHHHHHHHHHCCHHHHHHHEEEEEEEECCCCCEEEEEEEECHHCCCCCCCCHH
QIVADDLARSGQFSMTDVTGAAQPVEPSQVDYAAWRTKGAEAMVIGQVVALGGGRFEVRF
HHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHCCCCCEEEEEEEEEECCCEEEEEE
RLLDVVKGTQLAGYSYKITAAQWRATGHRIADVVYEKLTGIPGAFSSRIAYVQKQGKRYE
EEEEHHCCCEECCEEEEEEEHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEE
LRVADADGQNPRTIVRSLEPLISPMFSPDGTRLAYVSFEDKKPVVYVQSLQQGGRRKVAA
EEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEHHHCCCCEEEEE
FKGSNSAPAWSPDGRQLAVVLTRDGASQIYLINVDGSGLTRLMRSGDIDTEPVFSPDGQT
EECCCCCCCCCCCCCEEEEEEEECCCCEEEEEEECCHHHHHHHHCCCCCCCCCCCCCCCE
LYFTSDRGGSPQIYRVAKSGGDAKRVSFEGSYNVSPTISPDGRYLAYISRDGGRFRVVLQ
EEEECCCCCCCEEEEEECCCCCEEEEEECCCCCCCCEECCCCCEEEEEECCCCEEEEEHH
ELASGQTRVLTETTRDEAPSFAPNGQAVLYATVQGGRGVLGTVTLDGKTRARLSESGVDA
HHHCCCEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCHHHHHHCCCCC
REPAWGP
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA