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The map label for this gene is ecaA [H]

Identifier: 74318185

GI number: 74318185

Start: 2252086

End: 2252862

Strand: Direct

Name: ecaA [H]

Synonym: Tbd_2167

Alternate gene names: 74318185

Gene position: 2252086-2252862 (Clockwise)

Preceding gene: 74318184

Following gene: 74318186

Centisome position: 77.4

GC content: 65.89

Gene sequence:

>777_bases
ATGCTGTTTCTTACCCTCACATGGAGAAAACGCATGCTGCTTCGCCTGTCCGCCGCCCTTCTGTCCTCTCTAAGCTTCGC
CGCTGCCGCGGCACCGGGATCGCACTGGACCTACGAGGGCGAGCACGGCCCCACGCATTGGGCCCAACTCGACAAGGGCT
TTGCCGAATGCGCGCTCGGCCACGCGCAATCGCCGATCGACATCCGCGACGCGACGCCGAGGGCCGACGCGCCGGACCTC
GATTTCCGTTATCAGGCGACGCCGCTTCGTCTGATCAACAACGGCCATACCGTGCAGGTCGACGAATCGGATGCGGGCAC
GCTGACGATCGGCGGCCATCGCTACGCGCTCGCCCAGTTCCACTTCCATACGCCGAGCGAGGAACGTGTGCGCGGCAAGG
CCTACGACATGGTTGCGCATCTGGTCCACAAGGACGCTTCCGGGAAACTCGCCGCGGTCGCCGTGCTATTCAAGGCCGGC
CGCGAAAACGCTGCGCTGAAGACCGTGTTCGACAACATGCCGCTCGGCGCAGGGCCGGAGCATAAGGTCGAAGGCGTGCA
GTTCAACCCGGCCGACCTGCTGCCCAAGCAGCACGCCTACTACCACTTCCAGGGCTCGCTGACGACGCCGCCGTGTTCGG
AGGGTGTCGCCTGGTATGTGCTGAAGACCCCGGTCGAGGCCTCGCGTGCACAGATCGGCCAGTTGCGCACGCTCTACCCG
CACAACGCCCGCCCGGTGCAGGCGCTGCACGGCCGGCAGGTGATCGACCACCCCTGA

Upstream 100 bases:

>100_bases
CAGGGACCGCAAGCCGGCGACGATCCCGCCTAGGCTGCCGGAGCGCGGCCTTGACGTCCCGCTGACGCCGCCTTGACGCG
CGCGCGACAGGACTTCGCGC

Downstream 100 bases:

>100_bases
TTGCCGCGGGGCGTTCGCCGCACGGCGGGGGCGCGCCAGGGGATCTGTCACGCTGCGCAGAGCGGGCCCGCCGTGCTACT
GTTCACGCGGTTTCGTATCT

Product: carbonic anhydrase

Products: NA

Alternate protein names: Carbonate dehydratase [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLFLTLTWRKRMLLRLSAALLSSLSFAAAAAPGSHWTYEGEHGPTHWAQLDKGFAECALGHAQSPIDIRDATPRADAPDL
DFRYQATPLRLINNGHTVQVDESDAGTLTIGGHRYALAQFHFHTPSEERVRGKAYDMVAHLVHKDASGKLAAVAVLFKAG
RENAALKTVFDNMPLGAGPEHKVEGVQFNPADLLPKQHAYYHFQGSLTTPPCSEGVAWYVLKTPVEASRAQIGQLRTLYP
HNARPVQALHGRQVIDHP

Sequences:

>Translated_258_residues
MLFLTLTWRKRMLLRLSAALLSSLSFAAAAAPGSHWTYEGEHGPTHWAQLDKGFAECALGHAQSPIDIRDATPRADAPDL
DFRYQATPLRLINNGHTVQVDESDAGTLTIGGHRYALAQFHFHTPSEERVRGKAYDMVAHLVHKDASGKLAAVAVLFKAG
RENAALKTVFDNMPLGAGPEHKVEGVQFNPADLLPKQHAYYHFQGSLTTPPCSEGVAWYVLKTPVEASRAQIGQLRTLYP
HNARPVQALHGRQVIDHP
>Mature_258_residues
MLFLTLTWRKRMLLRLSAALLSSLSFAAAAAPGSHWTYEGEHGPTHWAQLDKGFAECALGHAQSPIDIRDATPRADAPDL
DFRYQATPLRLINNGHTVQVDESDAGTLTIGGHRYALAQFHFHTPSEERVRGKAYDMVAHLVHKDASGKLAAVAVLFKAG
RENAALKTVFDNMPLGAGPEHKVEGVQFNPADLLPKQHAYYHFQGSLTTPPCSEGVAWYVLKTPVEASRAQIGQLRTLYP
HNARPVQALHGRQVIDHP

Specific function: Reversible hydration of carbon dioxide [H]

COG id: COG3338

COG function: function code P; Carbonic anhydrase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-carbonic anhydrase family [H]

Homologues:

Organism=Homo sapiens, GI4885101, Length=256, Percent_Identity=35.546875, Blast_Score=120, Evalue=9e-28,
Organism=Homo sapiens, GI4557395, Length=256, Percent_Identity=35.15625, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI6912284, Length=265, Percent_Identity=34.7169811320755, Blast_Score=111, Evalue=8e-25,
Organism=Homo sapiens, GI258679498, Length=256, Percent_Identity=31.25, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI192447434, Length=256, Percent_Identity=31.25, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI192447432, Length=256, Percent_Identity=31.25, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI192447430, Length=256, Percent_Identity=31.25, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI4502517, Length=256, Percent_Identity=31.25, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI6005723, Length=288, Percent_Identity=31.9444444444444, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI62240989, Length=200, Percent_Identity=36.5, Blast_Score=104, Evalue=9e-23,
Organism=Homo sapiens, GI4502515, Length=237, Percent_Identity=33.7552742616034, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI38348436, Length=256, Percent_Identity=30.078125, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI45935383, Length=239, Percent_Identity=33.4728033472803, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI4502521, Length=232, Percent_Identity=32.7586206896552, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI4885099, Length=256, Percent_Identity=30.078125, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI22027500, Length=242, Percent_Identity=30.9917355371901, Blast_Score=94, Evalue=9e-20,
Organism=Homo sapiens, GI70167127, Length=235, Percent_Identity=31.4893617021277, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI4502519, Length=268, Percent_Identity=27.6119402985075, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI169636420, Length=257, Percent_Identity=32.295719844358, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI194097398, Length=271, Percent_Identity=26.9372693726937, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17568715, Length=250, Percent_Identity=32, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71995280, Length=275, Percent_Identity=30.9090909090909, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17569163, Length=228, Percent_Identity=28.5087719298246, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17569165, Length=223, Percent_Identity=28.6995515695067, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI17737413, Length=254, Percent_Identity=33.0708661417323, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21358169, Length=279, Percent_Identity=29.7491039426523, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24645604, Length=277, Percent_Identity=25.2707581227437, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24650523, Length=288, Percent_Identity=26.3888888888889, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001148
- InterPro:   IPR018338
- InterPro:   IPR018340
- InterPro:   IPR006311 [H]

Pfam domain/function: PF00194 Carb_anhydrase [H]

EC number: =4.2.1.1 [H]

Molecular weight: Translated: 28347; Mature: 28347

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: PS51144 ALPHA_CA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFLTLTWRKRMLLRLSAALLSSLSFAAAAAPGSHWTYEGEHGPTHWAQLDKGFAECALG
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHHHC
HAQSPIDIRDATPRADAPDLDFRYQATPLRLINNGHTVQVDESDAGTLTIGGHRYALAQF
CCCCCCCCCCCCCCCCCCCCCEEECCCCEEEECCCCEEEEECCCCCEEEECCCEEEEEEE
HFHTPSEERVRGKAYDMVAHLVHKDASGKLAAVAVLFKAGRENAALKTVFDNMPLGAGPE
EECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCC
HKVEGVQFNPADLLPKQHAYYHFQGSLTTPPCSEGVAWYVLKTPVEASRAQIGQLRTLYP
CCCCCEEECHHHCCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHCEEEECC
HNARPVQALHGRQVIDHP
CCCCCHHHHCCCCCCCCC
>Mature Secondary Structure
MLFLTLTWRKRMLLRLSAALLSSLSFAAAAAPGSHWTYEGEHGPTHWAQLDKGFAECALG
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHHHC
HAQSPIDIRDATPRADAPDLDFRYQATPLRLINNGHTVQVDESDAGTLTIGGHRYALAQF
CCCCCCCCCCCCCCCCCCCCCEEECCCCEEEECCCCEEEEECCCCCEEEECCCEEEEEEE
HFHTPSEERVRGKAYDMVAHLVHKDASGKLAAVAVLFKAGRENAALKTVFDNMPLGAGPE
EECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCC
HKVEGVQFNPADLLPKQHAYYHFQGSLTTPPCSEGVAWYVLKTPVEASRAQIGQLRTLYP
CCCCCEEECHHHCCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHCEEEECC
HNARPVQALHGRQVIDHP
CCCCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9006032; 11759840 [H]