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The map label for this gene is ygfZ [C]

Identifier: 74318116

GI number: 74318116

Start: 2188907

End: 2189971

Strand: Direct

Name: ygfZ [C]

Synonym: Tbd_2098

Alternate gene names: 74318116

Gene position: 2188907-2189971 (Clockwise)

Preceding gene: 74318114

Following gene: 74318117

Centisome position: 75.23

GC content: 67.61

Gene sequence:

>1065_bases
TTGCGCTTCATTGCCCCCTCTCGACTGGAAAGCACCGTGATCGAATCCTGGAAAAACATTCTGCTCACCCAAGGCGCGGC
AATCGAGAACGGCAGCGTCCTGCACTTCGGCGACCCGGCCGCCGAACGCGCTGCGGCGACCGGCGCCACGATCGTCGCCG
ACCTCTCGCAACTCGGTGTCATCGCGCTGCGCGGCGCCGACACGGCCGGTTTTCTCCAGGGCCAGCTCACCAACGACGTA
CGCAATCTACCAGCCGATGGCGCGCAATGGAACGGCTACTGCAGCCCGAAGGGGCGCTTGCTGGCCAATTTCCTCGCCTG
GCGAAACGGGGACGACTACTGCCTGCAGCTGTCGGGTGACATCCTCGCCGGCGTGCTGAAGCGGCTTTCGATGTTCATCC
TGCGCGCCGACGTGAAGGCGCGCGACGCGAGCGAGGAAACGGTGCGCCTTGTCGTTGCCGGCAAGGACGCCGCTGCGGCC
GTGCGCGCGGCGATGGGAGAGCTACCCGAGGCCGAGATGCGCACGATCGCGCTCGCGGCGGGCCAAGTGGTCCGCGTCGG
CGACGACAAGTTCGTGCTGTCGATCGCGCCCGAGCGCGCGGCCGAGGTGTGGCAAAACCTGACCCGGAGCGCAACGCCGG
TCGGTGCGCCGGTGTGGGACTGGATGCGGCTCAACGCCGGTATCCCGATGATCGTCGCCGCGACCCAGGAGCAGTTCGTG
CCGCAGATGGTCAACCTCGAGCTCATCGGCGGCGTGAGCTTCCAAAAGGGCTGCTACCCGGGACAGGAAATCGTCGCGCG
CAGCCAGTATTTAGGCAAGCTCAAGCGCCGCATGGTTCTGGCCCACGCCGACGCCGAAGCCGCGCCCGGTGACAGCCTCT
ACAGCGCTGACCTCGACGGCCAGGCCAGCGGAACCGTGGTGAACGCCGCGCCCGCCCCGGCTGGCGGCTTCGATCTGCTC
GCGGTCGTCCAGGTCGAAAGCGCGAACAGCCAGACCCTGCACCTGAAGTCCGCCGACGGCGTCGCACTCGACTTGCGGCC
GCTGCCTTACGCGCTTCCCGAGTAA

Upstream 100 bases:

>100_bases
TGTTGCACGGCGGCCAGCGCCAGAAGCAGGAAGGCCGCAAGCAACAGCCCCAAGCGGCGCGAGGGTTTGAGTTCAATCTC
ATGCATCAGCGTGTACACTT

Downstream 100 bases:

>100_bases
ATGGCGCACGCCGCCTACGTCTATTACCGGCTGCGGCCCGGCAGCGCGGCCGCGGCGGCCGGGCCGGTCGACGCCCTGCT
CGACGCGCTCGCGCCGCTCT

Product: glycine cleavage T-protein (aminomethyl transferase)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MRFIAPSRLESTVIESWKNILLTQGAAIENGSVLHFGDPAAERAAATGATIVADLSQLGVIALRGADTAGFLQGQLTNDV
RNLPADGAQWNGYCSPKGRLLANFLAWRNGDDYCLQLSGDILAGVLKRLSMFILRADVKARDASEETVRLVVAGKDAAAA
VRAAMGELPEAEMRTIALAAGQVVRVGDDKFVLSIAPERAAEVWQNLTRSATPVGAPVWDWMRLNAGIPMIVAATQEQFV
PQMVNLELIGGVSFQKGCYPGQEIVARSQYLGKLKRRMVLAHADAEAAPGDSLYSADLDGQASGTVVNAAPAPAGGFDLL
AVVQVESANSQTLHLKSADGVALDLRPLPYALPE

Sequences:

>Translated_354_residues
MRFIAPSRLESTVIESWKNILLTQGAAIENGSVLHFGDPAAERAAATGATIVADLSQLGVIALRGADTAGFLQGQLTNDV
RNLPADGAQWNGYCSPKGRLLANFLAWRNGDDYCLQLSGDILAGVLKRLSMFILRADVKARDASEETVRLVVAGKDAAAA
VRAAMGELPEAEMRTIALAAGQVVRVGDDKFVLSIAPERAAEVWQNLTRSATPVGAPVWDWMRLNAGIPMIVAATQEQFV
PQMVNLELIGGVSFQKGCYPGQEIVARSQYLGKLKRRMVLAHADAEAAPGDSLYSADLDGQASGTVVNAAPAPAGGFDLL
AVVQVESANSQTLHLKSADGVALDLRPLPYALPE
>Mature_354_residues
MRFIAPSRLESTVIESWKNILLTQGAAIENGSVLHFGDPAAERAAATGATIVADLSQLGVIALRGADTAGFLQGQLTNDV
RNLPADGAQWNGYCSPKGRLLANFLAWRNGDDYCLQLSGDILAGVLKRLSMFILRADVKARDASEETVRLVVAGKDAAAA
VRAAMGELPEAEMRTIALAAGQVVRVGDDKFVLSIAPERAAEVWQNLTRSATPVGAPVWDWMRLNAGIPMIVAATQEQFV
PQMVNLELIGGVSFQKGCYPGQEIVARSQYLGKLKRRMVLAHADAEAAPGDSLYSADLDGQASGTVVNAAPAPAGGFDLL
AVVQVESANSQTLHLKSADGVALDLRPLPYALPE

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication [H]

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family [H]

Homologues:

Organism=Escherichia coli, GI1789265, Length=321, Percent_Identity=28.3489096573209, Blast_Score=117, Evalue=9e-28,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017703
- InterPro:   IPR006222 [H]

Pfam domain/function: PF01571 GCV_T [H]

EC number: NA

Molecular weight: Translated: 37455; Mature: 37455

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFIAPSRLESTVIESWKNILLTQGAAIENGSVLHFGDPAAERAAATGATIVADLSQLGV
CCCCCCHHHHHHHHHHHHHHEEECCCEECCCCEEEECCCHHHHHHHCCHHHHHHHHHCCE
IALRGADTAGFLQGQLTNDVRNLPADGAQWNGYCSPKGRLLANFLAWRNGDDYCLQLSGD
EEEECCCCCHHHHCHHHHHHHCCCCCCCCCCCEECCCHHHHHHHHHHCCCCCEEEEECCH
ILAGVLKRLSMFILRADVKARDASEETVRLVVAGKDAAAAVRAAMGELPEAEMRTIALAA
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCHHHHHEEEEEC
GQVVRVGDDKFVLSIAPERAAEVWQNLTRSATPVGAPVWDWMRLNAGIPMIVAATQEQFV
CCEEEECCCEEEEEECCHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEEECCHHHHC
PQMVNLELIGGVSFQKGCYPGQEIVARSQYLGKLKRRMVLAHADAEAAPGDSLYSADLDG
HHHHCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCEEECCCCC
QASGTVVNAAPAPAGGFDLLAVVQVESANSQTLHLKSADGVALDLRPLPYALPE
CCCCCEEECCCCCCCCCEEEEEEEEECCCCCEEEEECCCCEEEECCCCCCCCCC
>Mature Secondary Structure
MRFIAPSRLESTVIESWKNILLTQGAAIENGSVLHFGDPAAERAAATGATIVADLSQLGV
CCCCCCHHHHHHHHHHHHHHEEECCCEECCCCEEEECCCHHHHHHHCCHHHHHHHHHCCE
IALRGADTAGFLQGQLTNDVRNLPADGAQWNGYCSPKGRLLANFLAWRNGDDYCLQLSGD
EEEECCCCCHHHHCHHHHHHHCCCCCCCCCCCEECCCHHHHHHHHHHCCCCCEEEEECCH
ILAGVLKRLSMFILRADVKARDASEETVRLVVAGKDAAAAVRAAMGELPEAEMRTIALAA
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCHHHHHEEEEEC
GQVVRVGDDKFVLSIAPERAAEVWQNLTRSATPVGAPVWDWMRLNAGIPMIVAATQEQFV
CCEEEECCCEEEEEECCHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEEECCHHHHC
PQMVNLELIGGVSFQKGCYPGQEIVARSQYLGKLKRRMVLAHADAEAAPGDSLYSADLDG
HHHHCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCEEECCCCC
QASGTVVNAAPAPAGGFDLLAVVQVESANSQTLHLKSADGVALDLRPLPYALPE
CCCCCEEECCCCCCCCCEEEEEEEEECCCCCEEEEECCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA