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The map label for this gene is panB

Identifier: 74318071

GI number: 74318071

Start: 2140541

End: 2141329

Strand: Direct

Name: panB

Synonym: Tbd_2053

Alternate gene names: 74318071

Gene position: 2140541-2141329 (Clockwise)

Preceding gene: 74318070

Following gene: 74318072

Centisome position: 73.56

GC content: 70.22

Gene sequence:

>789_bases
ATGCCGGTGACGCTCTCGACGCTAAAAGCGCTGCGGCAGAAGGGCGAGAAGATCGCCGTGCTCACGTGCTACGACGCGAG
CTTCGCGCGCGTGTTCGACGCCGCCGGCGTCGACGTCCTGCTCGTCGGCGATTCGCTCGGCATGGTGATCCAGGGCCACG
CCTCGACGCTGCCCGTCAAACTGGCCGAAATGGCGTATCACACGCGCTGCGTCGCGGCCGGAACGACGCGCGCCTTCATC
GTCGCCGACCTGCCTTTCGGCAGCTACCAGCCGTCGCCGGAGCGCGCCTACACGGCGGCCGCGCGCCTGATGGCGGCCGG
CGCGCACATGATCAAGCTCGAAGGCGGTGCGGTCATGGTCGACACGGTCGCGTTTCTTGCCGCGCGCGGCATTCCGGTGT
GCGCCCACCTCGGGCTTCTGCCGCAGTCGGTCAACCAGCTCGGCGGCTACCGGGTGCAGGGGCGCGAGGACGGCGACGCC
GCACAGCTCGTCGCCGACGCCCGCGCGCTCGAGGCAGCCGGTGCGGGACTGATCGTGCTCGAAATGGTGCCTGCGGCGCT
GGCGAAGACCGTCACCGCGGCGCTGTCGATGCCAACCATCGGCATCGGGGCCGGTGCCGACTGCGCGGGACAGGTCCTCG
TGTCCTACGACATGCTCGGCCTCTATCCGCGCGCGCCGAAGTTCTCGAAGAATTTTCTCGCCGGCGCCGGCAGCGTCGAC
GCCGCCGTGCGCGCCTACGTCGCGGCAGTCAAGGACGGCAGCTTCCCCGCCGCCGAACACGCGTTCTGA

Upstream 100 bases:

>100_bases
CACCTCAACTTCGCGCAGAACGAGGCCGATTTCGAGCTGCTGCTCGAACGCATGAACAAGATGCGCGGACCGCGCGAGTT
CTTCAGCCGGGCCGCGTGAC

Downstream 100 bases:

>100_bases
TGCAGGTCGTCCATACCGTCGCCGACCTGCGCGCGGCGCTTTCGGAAGCGGACCGCAGCGCATTCGTGCCGACGATGGGC
AACCTCCACGCCGGCCACGT

Product: ketopantoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MPVTLSTLKALRQKGEKIAVLTCYDASFARVFDAAGVDVLLVGDSLGMVIQGHASTLPVKLAEMAYHTRCVAAGTTRAFI
VADLPFGSYQPSPERAYTAAARLMAAGAHMIKLEGGAVMVDTVAFLAARGIPVCAHLGLLPQSVNQLGGYRVQGREDGDA
AQLVADARALEAAGAGLIVLEMVPAALAKTVTAALSMPTIGIGAGADCAGQVLVSYDMLGLYPRAPKFSKNFLAGAGSVD
AAVRAYVAAVKDGSFPAAEHAF

Sequences:

>Translated_262_residues
MPVTLSTLKALRQKGEKIAVLTCYDASFARVFDAAGVDVLLVGDSLGMVIQGHASTLPVKLAEMAYHTRCVAAGTTRAFI
VADLPFGSYQPSPERAYTAAARLMAAGAHMIKLEGGAVMVDTVAFLAARGIPVCAHLGLLPQSVNQLGGYRVQGREDGDA
AQLVADARALEAAGAGLIVLEMVPAALAKTVTAALSMPTIGIGAGADCAGQVLVSYDMLGLYPRAPKFSKNFLAGAGSVD
AAVRAYVAAVKDGSFPAAEHAF
>Mature_261_residues
PVTLSTLKALRQKGEKIAVLTCYDASFARVFDAAGVDVLLVGDSLGMVIQGHASTLPVKLAEMAYHTRCVAAGTTRAFIV
ADLPFGSYQPSPERAYTAAARLMAAGAHMIKLEGGAVMVDTVAFLAARGIPVCAHLGLLPQSVNQLGGYRVQGREDGDAA
QLVADARALEAAGAGLIVLEMVPAALAKTVTAALSMPTIGIGAGADCAGQVLVSYDMLGLYPRAPKFSKNFLAGAGSVDA
AVRAYVAAVKDGSFPAAEHAF

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=262, Percent_Identity=53.4351145038168, Blast_Score=279, Evalue=1e-76,
Organism=Saccharomyces cerevisiae, GI6319653, Length=273, Percent_Identity=36.2637362637363, Blast_Score=164, Evalue=1e-41,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_THIDA (Q3SH83)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315811.1
- ProteinModelPortal:   Q3SH83
- SMR:   Q3SH83
- STRING:   Q3SH83
- GeneID:   3672224
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_2053
- NMPDR:   fig|292415.3.peg.2193
- eggNOG:   COG0413
- HOGENOM:   HBG299908
- OMA:   AKFVRNF
- PhylomeDB:   Q3SH83
- BioCyc:   TDEN292415:TBD_2053-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 26878; Mature: 26747

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: ACT_SITE 181-181 BINDING 83-83 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVTLSTLKALRQKGEKIAVLTCYDASFARVFDAAGVDVLLVGDSLGMVIQGHASTLPVK
CCCCHHHHHHHHHCCCEEEEEEEECCHHHHHHHCCCCEEEEECCCCCEEEECCCCCCCHH
LAEMAYHTRCVAAGTTRAFIVADLPFGSYQPSPERAYTAAARLMAAGAHMIKLEGGAVMV
HHHHHHHHHEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCEEEH
DTVAFLAARGIPVCAHLGLLPQSVNQLGGYRVQGREDGDAAQLVADARALEAAGAGLIVL
HHHHHHHHCCCHHHHHHCCCHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEE
EMVPAALAKTVTAALSMPTIGIGAGADCAGQVLVSYDMLGLYPRAPKFSKNFLAGAGSVD
HHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCHH
AAVRAYVAAVKDGSFPAAEHAF
HHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PVTLSTLKALRQKGEKIAVLTCYDASFARVFDAAGVDVLLVGDSLGMVIQGHASTLPVK
CCCHHHHHHHHHCCCEEEEEEEECCHHHHHHHCCCCEEEEECCCCCEEEECCCCCCCHH
LAEMAYHTRCVAAGTTRAFIVADLPFGSYQPSPERAYTAAARLMAAGAHMIKLEGGAVMV
HHHHHHHHHEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCEEEH
DTVAFLAARGIPVCAHLGLLPQSVNQLGGYRVQGREDGDAAQLVADARALEAAGAGLIVL
HHHHHHHHCCCHHHHHHCCCHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEE
EMVPAALAKTVTAALSMPTIGIGAGADCAGQVLVSYDMLGLYPRAPKFSKNFLAGAGSVD
HHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCHH
AAVRAYVAAVKDGSFPAAEHAF
HHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA