Click here to switch to the map view.

The map label for this gene is dhfrIII [H]

Identifier: 74318057

GI number: 74318057

Start: 2128195

End: 2128698

Strand: Reverse

Name: dhfrIII [H]

Synonym: Tbd_2039

Alternate gene names: 74318057

Gene position: 2128698-2128195 (Counterclockwise)

Preceding gene: 74318058

Following gene: 74318056

Centisome position: 73.16

GC content: 68.45

Gene sequence:

>504_bases
ATGAGCGGGACGGGGCAGCCGCGCGTGAGCGTCATCGCCGCGCTGGCAAAGAATCGCGTCATCGGCATCGAGAACCGGCT
GCCGTGGCGTCTGCCCGAAGACCTCGCCCACTTCAAGGCGCTGACGCTCGACCATCCCGTCCTGATGGGGCGCAAGACCT
TCGAGTCGCTCGGGCGGCCCTTGCCGCGGCGCACCAACGTCGTCATCACCCGCAACGCCGACTACCGGCCCGACGGCTGC
CTCGTCGCGGCGTCGATGCCGGCGGCGATCGCGCTATGCCAGGGCGCCGACGAAATCTTCTTCATCGGCGGCGCCGAGCT
CTATGCGCAGGCGATTCCGCTCGCCGATCGCCTGTATCTGACCGAAGTCGACGCCGAGCCCGAGGGCGATGCCTGGTTTC
CCGAGTTCGACCGGGCGGCGTTCCGCGAAATCTCGCGCGCCTCGCACGTGGGCGAGAAAGGCGACGTGCTGCGCTTCGAC
TTCGTCGTGTACGAACGCCGCTGA

Upstream 100 bases:

>100_bases
CGCTCAATCCCGACGTGACCGACATCTTCGGCTTTCGCTTCGAGGATTTCACGCTCGGCGGCTACGACCCGCACCCGGCG
ATCAAGGCGCCGGTCGCGGT

Downstream 100 bases:

>100_bases
CGGCCGAGGGGCGGAACACGGGCAGCCCCCCGCTGCGCTTGCGCCAAGCCGCTTTGACAGGCCGCGTCGCATCGACTAGA
AAAACATACAAGGCCCGGCG

Product: dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MSGTGQPRVSVIAALAKNRVIGIENRLPWRLPEDLAHFKALTLDHPVLMGRKTFESLGRPLPRRTNVVITRNADYRPDGC
LVAASMPAAIALCQGADEIFFIGGAELYAQAIPLADRLYLTEVDAEPEGDAWFPEFDRAAFREISRASHVGEKGDVLRFD
FVVYERR

Sequences:

>Translated_167_residues
MSGTGQPRVSVIAALAKNRVIGIENRLPWRLPEDLAHFKALTLDHPVLMGRKTFESLGRPLPRRTNVVITRNADYRPDGC
LVAASMPAAIALCQGADEIFFIGGAELYAQAIPLADRLYLTEVDAEPEGDAWFPEFDRAAFREISRASHVGEKGDVLRFD
FVVYERR
>Mature_166_residues
SGTGQPRVSVIAALAKNRVIGIENRLPWRLPEDLAHFKALTLDHPVLMGRKTFESLGRPLPRRTNVVITRNADYRPDGCL
VAASMPAAIALCQGADEIFFIGGAELYAQAIPLADRLYLTEVDAEPEGDAWFPEFDRAAFREISRASHVGEKGDVLRFDF
VVYERR

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=159, Percent_Identity=44.0251572327044, Blast_Score=135, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17505899, Length=182, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324810, Length=213, Percent_Identity=29.5774647887324, Blast_Score=75, Evalue=5e-15,
Organism=Drosophila melanogaster, GI116008026, Length=179, Percent_Identity=29.0502793296089, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24647458, Length=179, Percent_Identity=29.0502793296089, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18596; Mature: 18465

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGTGQPRVSVIAALAKNRVIGIENRLPWRLPEDLAHFKALTLDHPVLMGRKTFESLGRP
CCCCCCHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHCCCCCHHHCHHHHHHHCCC
LPRRTNVVITRNADYRPDGCLVAASMPAAIALCQGADEIFFIGGAELYAQAIPLADRLYL
CCCCCCEEEEECCCCCCCCEEEEECCCHHHHHHCCCCEEEEEECHHHHHHHCCCCCEEEE
TEVDAEPEGDAWFPEFDRAAFREISRASHVGEKGDVLRFDFVVYERR
EECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECC
>Mature Secondary Structure 
SGTGQPRVSVIAALAKNRVIGIENRLPWRLPEDLAHFKALTLDHPVLMGRKTFESLGRP
CCCCCHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHCCCCCHHHCHHHHHHHCCC
LPRRTNVVITRNADYRPDGCLVAASMPAAIALCQGADEIFFIGGAELYAQAIPLADRLYL
CCCCCCEEEEECCCCCCCCEEEEECCCHHHHHHCCCCEEEEEECHHHHHHHCCCCCEEEE
TEVDAEPEGDAWFPEFDRAAFREISRASHVGEKGDVLRFDFVVYERR
EECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]