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The map label for this gene is thyA

Identifier: 74318058

GI number: 74318058

Start: 2128695

End: 2129489

Strand: Reverse

Name: thyA

Synonym: Tbd_2040

Alternate gene names: 74318058

Gene position: 2129489-2128695 (Counterclockwise)

Preceding gene: 74318059

Following gene: 74318057

Centisome position: 73.18

GC content: 63.52

Gene sequence:

>795_bases
ATGAAACCCTATCTCGACCTGATGCGCCACGTGCTTGAGCACGGCACACGCAAAGACGACCGCACCGGCACCGGCACCCT
GTCGGTCTTCGGCTGGCAGACGCGCTACGACCTTGCCGCGGGCTTTCCGCTCGTCACGACCAAGAAATGTCACCTGCGCT
CGATCGTTCACGAACTGCTATGGTTTCTCCAGGGCGACACGAACATCCGCTATCTGAAGGAAAACGGCGTCTCGATCTGG
GACGAGTGGGCCGACGAGAACGGCGATCTCGGCCCGGTCTACGGCCATCAGTGGCGTTCGTGGCCTAAAGCCGACGGCGG
GGTGATCGACCAGATCGCCGAGGCCGTGAAGACGCTCAGGACCAACCCCGATTCGCGCCGCATCATCGTCTCGGCGTGGA
ACGTTGCCGACCTCGACAGGATGGCGCTGGCGCCCTGCCACGCGTTCTTCCAGTTCTACGTTGCCGAAGGCAGGTTGAGT
TGCCAGCTCTACCAGCGCAGCGCCGACATCTTCCTCGGCGTGCCGTTCAACATCGCCTCCTACGCGCTGCTGACACTGAT
GATGGCGCAGGTGACGGGCCTGAAGCCGGGTGACTTCGTACATACGCTCGGTGACGCCCACCTCTACGTGAACCACTTGG
AGCAGGCGCGCGAGCAGCTTTCCCGCGAACCGCGCCCGCTGCCCACGATGACGCTCAATCCCGACGTGACCGACATCTTC
GGCTTTCGCTTCGAGGATTTCACGCTCGGCGGCTACGACCCGCACCCGGCGATCAAGGCGCCGGTCGCGGTATGA

Upstream 100 bases:

>100_bases
CGCGCCTCGCCGGGTTTTTCGACGGCGCACCGCCCGCCGTACCGGCGGTCGCCGGCGGGTCGATCCAGGCCGCCTCCGCC
AACCCCAATCCAGGTTCTCC

Downstream 100 bases:

>100_bases
GCGGGACGGGGCAGCCGCGCGTGAGCGTCATCGCCGCGCTGGCAAAGAATCGCGTCATCGGCATCGAGAACCGGCTGCCG
TGGCGTCTGCCCGAAGACCT

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKPYLDLMRHVLEHGTRKDDRTGTGTLSVFGWQTRYDLAAGFPLVTTKKCHLRSIVHELLWFLQGDTNIRYLKENGVSIW
DEWADENGDLGPVYGHQWRSWPKADGGVIDQIAEAVKTLRTNPDSRRIIVSAWNVADLDRMALAPCHAFFQFYVAEGRLS
CQLYQRSADIFLGVPFNIASYALLTLMMAQVTGLKPGDFVHTLGDAHLYVNHLEQAREQLSREPRPLPTMTLNPDVTDIF
GFRFEDFTLGGYDPHPAIKAPVAV

Sequences:

>Translated_264_residues
MKPYLDLMRHVLEHGTRKDDRTGTGTLSVFGWQTRYDLAAGFPLVTTKKCHLRSIVHELLWFLQGDTNIRYLKENGVSIW
DEWADENGDLGPVYGHQWRSWPKADGGVIDQIAEAVKTLRTNPDSRRIIVSAWNVADLDRMALAPCHAFFQFYVAEGRLS
CQLYQRSADIFLGVPFNIASYALLTLMMAQVTGLKPGDFVHTLGDAHLYVNHLEQAREQLSREPRPLPTMTLNPDVTDIF
GFRFEDFTLGGYDPHPAIKAPVAV
>Mature_264_residues
MKPYLDLMRHVLEHGTRKDDRTGTGTLSVFGWQTRYDLAAGFPLVTTKKCHLRSIVHELLWFLQGDTNIRYLKENGVSIW
DEWADENGDLGPVYGHQWRSWPKADGGVIDQIAEAVKTLRTNPDSRRIIVSAWNVADLDRMALAPCHAFFQFYVAEGRLS
CQLYQRSADIFLGVPFNIASYALLTLMMAQVTGLKPGDFVHTLGDAHLYVNHLEQAREQLSREPRPLPTMTLNPDVTDIF
GFRFEDFTLGGYDPHPAIKAPVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=281, Percent_Identity=54.0925266903915, Blast_Score=306, Evalue=1e-83,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=70.8333333333333, Blast_Score=402, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI71993377, Length=282, Percent_Identity=48.5815602836879, Blast_Score=271, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6324648, Length=290, Percent_Identity=42.7586206896552, Blast_Score=217, Evalue=2e-57,
Organism=Drosophila melanogaster, GI17137556, Length=283, Percent_Identity=53.356890459364, Blast_Score=303, Evalue=8e-83,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_THIDA (Q3SH96)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315798.1
- HSSP:   P0A884
- ProteinModelPortal:   Q3SH96
- SMR:   Q3SH96
- STRING:   Q3SH96
- GeneID:   3672211
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_2040
- NMPDR:   fig|292415.3.peg.2180
- eggNOG:   COG0207
- HOGENOM:   HBG588098
- OMA:   RMALAPC
- PhylomeDB:   Q3SH96
- BioCyc:   TDEN292415:TBD_2040-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 29887; Mature: 29887

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 146-146

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPYLDLMRHVLEHGTRKDDRTGTGTLSVFGWQTRYDLAAGFPLVTTKKCHLRSIVHELL
CCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHCCCCCEECCHHHHHHHHHHHH
WFLQGDTNIRYLKENGVSIWDEWADENGDLGPVYGHQWRSWPKADGGVIDQIAEAVKTLR
HHHCCCCCEEEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TNPDSRRIIVSAWNVADLDRMALAPCHAFFQFYVAEGRLSCQLYQRSADIFLGVPFNIAS
CCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEECCHHHHH
YALLTLMMAQVTGLKPGDFVHTLGDAHLYVNHLEQAREQLSREPRPLPTMTLNPDVTDIF
HHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHH
GFRFEDFTLGGYDPHPAIKAPVAV
CCEEECEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MKPYLDLMRHVLEHGTRKDDRTGTGTLSVFGWQTRYDLAAGFPLVTTKKCHLRSIVHELL
CCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHCCCCCEECCHHHHHHHHHHHH
WFLQGDTNIRYLKENGVSIWDEWADENGDLGPVYGHQWRSWPKADGGVIDQIAEAVKTLR
HHHCCCCCEEEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TNPDSRRIIVSAWNVADLDRMALAPCHAFFQFYVAEGRLSCQLYQRSADIFLGVPFNIAS
CCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEECCHHHHH
YALLTLMMAQVTGLKPGDFVHTLGDAHLYVNHLEQAREQLSREPRPLPTMTLNPDVTDIF
HHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHH
GFRFEDFTLGGYDPHPAIKAPVAV
CCEEECEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA