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The map label for this gene is pleD [H]

Identifier: 74318041

GI number: 74318041

Start: 2111574

End: 2113472

Strand: Direct

Name: pleD [H]

Synonym: Tbd_2023

Alternate gene names: 74318041

Gene position: 2111574-2113472 (Clockwise)

Preceding gene: 74318040

Following gene: 74318043

Centisome position: 72.57

GC content: 68.88

Gene sequence:

>1899_bases
ATGACTCAGCTCGACCAACTAAAACTCAGTGGGCAGTTGCCTTCGCCGCGCGGCGTCGCGCTCGCCGTGCTCGAGTTGTC
GCGGCGCGACAATGCGACGCTCGGCGAAATCGCGCGCGTCGTACAGACCGACCCCGCGCTGTCCGGGCGCCTGATCAAGC
TCGCCAATTCGGCTTCGCACATCGCGCGTCCCGTGGTCTCCGTGCAGGAAGCCGTGGTGCGTCAGGGCATGTCGACGGTG
CGCCAGCTCGCGCTCGGATTTTCGCTTCTCGATCAGTTCCGGAACGGCGCCTGTACGGCCTTCGACTATCAGGAATACTG
GTCGCACTCGCTGCTCATGGCGCTGTCGCTGCAGGCGCTGGGCGCCCGCGTCCACGTCGCGGCGGCCGACGAATTGTTCA
TCTGCGGCCTGCTCGCGCAAGTCGGGCGCCTCGCACTCGCGACGGCCTATCCCGCCGAGTACGACGCCGTCCTGCACGCC
CATCGCACCGATCCGTCGAAGTCGCTGATCACGCTCGAGCGCTCCCGCCTCGAAATCGACCACATCGAACTCGGGATCGT
GATGATGACCGACTGGGGCATGCCGCGCGTCTTCACGATTCCGCTCGCGCACTACCAGGACCCGGAATACACGAGCTTTC
CGCACGACTCGCGCGCCAACAGCCTCATGCTGATGCTGCGCCTGTCCCACCGGCTGGCCGATCTCGCCTTGGGTAGCGCC
GACGGGCGCATGCAGCTGGCGCGCGAGTGGATGACACTCGCGACCGAACTCGACCTCCCGCCGGAAAACGCCGGCAGCTT
CATCGACGAGGTGATCGCGCAATGGCACGACTGGGGCGCGCTCCTGAAGATCCCCACCTCGGTCCTGCCGCCGTTCGCCG
AGATCAGCCACGGCAGCGCCGAGGCGAGCGACGACGAGGCGCCGCTGCGCATCGTCGTCGCCGACGGCAATGCCTTCACG
CGGCGCAAGATCATGGCCTTGCTCGTCGAGGACAGCGGCCACGTCGTCTATCCGGCCGATAACGGCAATAGCGCGCTCGC
CCTGGCGATGGAGGTGTTGCCGCACGTTATCATCGCCCACATCAACCTGCCGCTGCTCGACGGACTCGAGCTGTGCCAGG
CCTTGCGCGCGACCGATGAGGGCCGTCGCATGCACATCCTGCTGATGTCGGACGAGCACGCCGAGGACCAGCTCGCCCGC
GCCTACGAGGCTGGCGCCGACGGCTACGCGCACAGCGCGCTCAGCGCCAAGAGCCTGCGCACGCGGCTGTGCGCGGCGCA
GCGCCTGCTGCAACTGCAGAACGCGTGGGAGCGCGACCGCGCGCAGTTGCGCCAGATCGCGGCCGAACTGGCCGTCGCCA
ACCGCCGCCTCGCCAACGCCGCGCTGACCGACGCGCTGACCGGTCTGCCCAACCGCCGCTCGGCGATGGACCAGCTCGAG
CAGGCCTGGAGCGCCGCGGCGCGCGCCGACACACCGCTCGCGGTCATGGTCGTCGACATCGATCACTTCAAGCACATCAA
CGACACCCACGGACACGCCCTCGGCGACAAGGTGCTGCGCGAGGCCGCGAATGCGCTGCGCGCGTCGTCGCGCCGGGAGG
ACAGCGTGTGCCGGATCGGCGGCGAGGAGTTCCTGGTCATCTGTCCGAACACCGATCTCGCTGCGGCCATGCAATCGGCC
GAACGCCTGCGGGCCACGCTGAGCGCCCGTCGCATCGCCGTGGGCGAGGCCGAGTGCACGATCACCGCGAGCATCGGCGT
GGCCGTGCGCGAATACGACACGGCCGACATCGACGGCCTCGTCAGCGCGGCGGATCAGGCGCTCTATGCCGCCAAGGAAG
CGGGCCGCAACCAGGTCTGCACCCAGCGCCGCCGGCCGGTCGCGGTCGAAGCGCCGTAG

Upstream 100 bases:

>100_bases
GCCGCACCCGCCCGTCCCCGTCGTCGATCGAGCCGGCGCGCGCCAGCGCGGCCGCACCGCATGGATGACGACTGCGCGTG
TTCCATAAGACCGATAACCG

Downstream 100 bases:

>100_bases
CCGCGCTCAGAGCGCACGCGAAACCCGGCGCACCGCCTCGTCCTCGGGGTGAGGCTCGATGCGAAAGCCGAGGCTTCCCA
CCAGCTTCAGCATCGGACGG

Product: response regulator receiver modulated diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 632; Mature: 631

Protein sequence:

>632_residues
MTQLDQLKLSGQLPSPRGVALAVLELSRRDNATLGEIARVVQTDPALSGRLIKLANSASHIARPVVSVQEAVVRQGMSTV
RQLALGFSLLDQFRNGACTAFDYQEYWSHSLLMALSLQALGARVHVAAADELFICGLLAQVGRLALATAYPAEYDAVLHA
HRTDPSKSLITLERSRLEIDHIELGIVMMTDWGMPRVFTIPLAHYQDPEYTSFPHDSRANSLMLMLRLSHRLADLALGSA
DGRMQLAREWMTLATELDLPPENAGSFIDEVIAQWHDWGALLKIPTSVLPPFAEISHGSAEASDDEAPLRIVVADGNAFT
RRKIMALLVEDSGHVVYPADNGNSALALAMEVLPHVIIAHINLPLLDGLELCQALRATDEGRRMHILLMSDEHAEDQLAR
AYEAGADGYAHSALSAKSLRTRLCAAQRLLQLQNAWERDRAQLRQIAAELAVANRRLANAALTDALTGLPNRRSAMDQLE
QAWSAAARADTPLAVMVVDIDHFKHINDTHGHALGDKVLREAANALRASSRREDSVCRIGGEEFLVICPNTDLAAAMQSA
ERLRATLSARRIAVGEAECTITASIGVAVREYDTADIDGLVSAADQALYAAKEAGRNQVCTQRRRPVAVEAP

Sequences:

>Translated_632_residues
MTQLDQLKLSGQLPSPRGVALAVLELSRRDNATLGEIARVVQTDPALSGRLIKLANSASHIARPVVSVQEAVVRQGMSTV
RQLALGFSLLDQFRNGACTAFDYQEYWSHSLLMALSLQALGARVHVAAADELFICGLLAQVGRLALATAYPAEYDAVLHA
HRTDPSKSLITLERSRLEIDHIELGIVMMTDWGMPRVFTIPLAHYQDPEYTSFPHDSRANSLMLMLRLSHRLADLALGSA
DGRMQLAREWMTLATELDLPPENAGSFIDEVIAQWHDWGALLKIPTSVLPPFAEISHGSAEASDDEAPLRIVVADGNAFT
RRKIMALLVEDSGHVVYPADNGNSALALAMEVLPHVIIAHINLPLLDGLELCQALRATDEGRRMHILLMSDEHAEDQLAR
AYEAGADGYAHSALSAKSLRTRLCAAQRLLQLQNAWERDRAQLRQIAAELAVANRRLANAALTDALTGLPNRRSAMDQLE
QAWSAAARADTPLAVMVVDIDHFKHINDTHGHALGDKVLREAANALRASSRREDSVCRIGGEEFLVICPNTDLAAAMQSA
ERLRATLSARRIAVGEAECTITASIGVAVREYDTADIDGLVSAADQALYAAKEAGRNQVCTQRRRPVAVEAP
>Mature_631_residues
TQLDQLKLSGQLPSPRGVALAVLELSRRDNATLGEIARVVQTDPALSGRLIKLANSASHIARPVVSVQEAVVRQGMSTVR
QLALGFSLLDQFRNGACTAFDYQEYWSHSLLMALSLQALGARVHVAAADELFICGLLAQVGRLALATAYPAEYDAVLHAH
RTDPSKSLITLERSRLEIDHIELGIVMMTDWGMPRVFTIPLAHYQDPEYTSFPHDSRANSLMLMLRLSHRLADLALGSAD
GRMQLAREWMTLATELDLPPENAGSFIDEVIAQWHDWGALLKIPTSVLPPFAEISHGSAEASDDEAPLRIVVADGNAFTR
RKIMALLVEDSGHVVYPADNGNSALALAMEVLPHVIIAHINLPLLDGLELCQALRATDEGRRMHILLMSDEHAEDQLARA
YEAGADGYAHSALSAKSLRTRLCAAQRLLQLQNAWERDRAQLRQIAAELAVANRRLANAALTDALTGLPNRRSAMDQLEQ
AWSAAARADTPLAVMVVDIDHFKHINDTHGHALGDKVLREAANALRASSRREDSVCRIGGEEFLVICPNTDLAAAMQSAE
RLRATLSARRIAVGEAECTITASIGVAVREYDTADIDGLVSAADQALYAAKEAGRNQVCTQRRRPVAVEAP

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=215, Percent_Identity=32.093023255814, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI87082007, Length=180, Percent_Identity=38.8888888888889, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1787262, Length=172, Percent_Identity=37.7906976744186, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787541, Length=207, Percent_Identity=32.8502415458937, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI87081881, Length=164, Percent_Identity=39.6341463414634, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786584, Length=174, Percent_Identity=34.4827586206897, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1787816, Length=157, Percent_Identity=36.3057324840764, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87081977, Length=210, Percent_Identity=30.952380952381, Blast_Score=94, Evalue=4e-20,
Organism=Escherichia coli, GI1787802, Length=185, Percent_Identity=31.8918918918919, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1788085, Length=182, Percent_Identity=33.5164835164835, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1788381, Length=167, Percent_Identity=31.7365269461078, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081974, Length=152, Percent_Identity=32.8947368421053, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI87081921, Length=155, Percent_Identity=29.6774193548387, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1788956, Length=180, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787056, Length=167, Percent_Identity=31.7365269461078, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 68803; Mature: 68672

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLDQLKLSGQLPSPRGVALAVLELSRRDNATLGEIARVVQTDPALSGRLIKLANSASH
CCCCCCEEECCCCCCCCCCEEEEEEHHCCCCCCHHHHHHHHHCCCCCCCCEEEECCCHHH
IARPVVSVQEAVVRQGMSTVRQLALGFSLLDQFRNGACTAFDYQEYWSHSLLMALSLQAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHH
GARVHVAAADELFICGLLAQVGRLALATAYPAEYDAVLHAHRTDPSKSLITLERSRLEID
CCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEHHHHCEEE
HIELGIVMMTDWGMPRVFTIPLAHYQDPEYTSFPHDSRANSLMLMLRLSHRLADLALGSA
EEEEEEEEEECCCCCEEEEEEHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
DGRMQLAREWMTLATELDLPPENAGSFIDEVIAQWHDWGALLKIPTSVLPPFAEISHGSA
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHEECCHHHCCCHHHHCCCCC
EASDDEAPLRIVVADGNAFTRRKIMALLVEDSGHVVYPADNGNSALALAMEVLPHVIIAH
CCCCCCCCEEEEEECCCCHHHHHEEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHH
INLPLLDGLELCQALRATDEGRRMHILLMSDEHAEDQLARAYEAGADGYAHSALSAKSLR
CCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
TRLCAAQRLLQLQNAWERDRAQLRQIAAELAVANRRLANAALTDALTGLPNRRSAMDQLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
QAWSAAARADTPLAVMVVDIDHFKHINDTHGHALGDKVLREAANALRASSRREDSVCRIG
HHHHHHHCCCCCEEEEEEEHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEC
GEEFLVICPNTDLAAAMQSAERLRATLSARRIAVGEAECTITASIGVAVREYDTADIDGL
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEECCEEEEECCCCCHHHH
VSAADQALYAAKEAGRNQVCTQRRRPVAVEAP
HHHHHHHHHHHHHCCCHHHHHHCCCCEEECCC
>Mature Secondary Structure 
TQLDQLKLSGQLPSPRGVALAVLELSRRDNATLGEIARVVQTDPALSGRLIKLANSASH
CCCCCEEECCCCCCCCCCEEEEEEHHCCCCCCHHHHHHHHHCCCCCCCCEEEECCCHHH
IARPVVSVQEAVVRQGMSTVRQLALGFSLLDQFRNGACTAFDYQEYWSHSLLMALSLQAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHH
GARVHVAAADELFICGLLAQVGRLALATAYPAEYDAVLHAHRTDPSKSLITLERSRLEID
CCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEHHHHCEEE
HIELGIVMMTDWGMPRVFTIPLAHYQDPEYTSFPHDSRANSLMLMLRLSHRLADLALGSA
EEEEEEEEEECCCCCEEEEEEHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
DGRMQLAREWMTLATELDLPPENAGSFIDEVIAQWHDWGALLKIPTSVLPPFAEISHGSA
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHEECCHHHCCCHHHHCCCCC
EASDDEAPLRIVVADGNAFTRRKIMALLVEDSGHVVYPADNGNSALALAMEVLPHVIIAH
CCCCCCCCEEEEEECCCCHHHHHEEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHH
INLPLLDGLELCQALRATDEGRRMHILLMSDEHAEDQLARAYEAGADGYAHSALSAKSLR
CCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
TRLCAAQRLLQLQNAWERDRAQLRQIAAELAVANRRLANAALTDALTGLPNRRSAMDQLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
QAWSAAARADTPLAVMVVDIDHFKHINDTHGHALGDKVLREAANALRASSRREDSVCRIG
HHHHHHHCCCCCEEEEEEEHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEC
GEEFLVICPNTDLAAAMQSAERLRATLSARRIAVGEAECTITASIGVAVREYDTADIDGL
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEECCEEEEECCCCCHHHH
VSAADQALYAAKEAGRNQVCTQRRRPVAVEAP
HHHHHHHHHHHHHCCCHHHHHHCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA