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The map label for this gene is rnfE [H]

Identifier: 74318038

GI number: 74318038

Start: 2109589

End: 2110266

Strand: Direct

Name: rnfE [H]

Synonym: Tbd_2020

Alternate gene names: 74318038

Gene position: 2109589-2110266 (Clockwise)

Preceding gene: 74318037

Following gene: 74318039

Centisome position: 72.5

GC content: 66.22

Gene sequence:

>678_bases
ATGATGACCCTGAGCGAATTCCGCAGCCTCTTCCACAACGGCCTCGTCAAGCAGAACACGGGACTCGTGCAACTGCTCGG
CCTGTGCCCGCTGCTCGCGATCAGCAACACCGTCGTCAACGCCCTGTCGCTCGGCCTGGCGACGATGCTCGTGATGGCGG
CGTCGAGCGGCGCGGTTTCGGCCGCCCGCAATTTCGTTCCGAACGAAATCCGCATCCCGGTCTTCGTCCTCATCATCGCG
GCGCTGGTGACCGTGATCGACCTCGCGATGAACGCCTTCGTCCATCCGCTCTACCTCGTGCTCGGCATTTTCATTCCGCT
CATCACGACCAACTGCATCGTGCTCGCCCGCGCCGACGCCTTCGCCGCCAAGAACCGGCCGCTGCACAGCGTCGTCGACG
CGGTCGCGATGGGCCTCGGGCTGACCCTGGTGCTGATCGTGCTCGGCGCGATGCGCGAGCTCGTCGGACAGGGCACGCTG
TTCTCGGGCATCGACCTGGTCTTCGGTGAAGACGCGAAGCAGTTCGTGCTGCATGTGCTGCCCGATTACCGCGGCTTCCT
GCTCGCGGTGCTGCCGCCCGGCGCCTTCATTGCGCTCGGCCTGCTGATCGCCGCGCACAACTGGAACAAGGCGCGCGCCG
AGCAGCGCGCGCAGCTGACCGTTCCCGCCACGGCTTGA

Upstream 100 bases:

>100_bases
CGCCGCGCGCGGTGGTCAAGGCGGTTCGCGGCGCGCTGGAATATTTTGCCCGGCACCGCAAGACCCTCGCGGCGCCGGTG
ACCCGAGAGGAGAAAGACGC

Downstream 100 bases:

>100_bases
GGCCGGGGCCGATGCCCGTGCCGAACGATGAACGCTGACAAACGCCGCGAGATTTTCCGCCGCCTGCGCGAAGCGAATCC
CCACCCGACGACCGAGCTCG

Product: electron transport complex RsxE subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MMTLSEFRSLFHNGLVKQNTGLVQLLGLCPLLAISNTVVNALSLGLATMLVMAASSGAVSAARNFVPNEIRIPVFVLIIA
ALVTVIDLAMNAFVHPLYLVLGIFIPLITTNCIVLARADAFAAKNRPLHSVVDAVAMGLGLTLVLIVLGAMRELVGQGTL
FSGIDLVFGEDAKQFVLHVLPDYRGFLLAVLPPGAFIALGLLIAAHNWNKARAEQRAQLTVPATA

Sequences:

>Translated_225_residues
MMTLSEFRSLFHNGLVKQNTGLVQLLGLCPLLAISNTVVNALSLGLATMLVMAASSGAVSAARNFVPNEIRIPVFVLIIA
ALVTVIDLAMNAFVHPLYLVLGIFIPLITTNCIVLARADAFAAKNRPLHSVVDAVAMGLGLTLVLIVLGAMRELVGQGTL
FSGIDLVFGEDAKQFVLHVLPDYRGFLLAVLPPGAFIALGLLIAAHNWNKARAEQRAQLTVPATA
>Mature_225_residues
MMTLSEFRSLFHNGLVKQNTGLVQLLGLCPLLAISNTVVNALSLGLATMLVMAASSGAVSAARNFVPNEIRIPVFVLIIA
ALVTVIDLAMNAFVHPLYLVLGIFIPLITTNCIVLARADAFAAKNRPLHSVVDAVAMGLGLTLVLIVLGAMRELVGQGTL
FSGIDLVFGEDAKQFVLHVLPDYRGFLLAVLPPGAFIALGLLIAAHNWNKARAEQRAQLTVPATA

Specific function: May be part of a membrane complex involved in electron transport [H]

COG id: COG4660

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfE

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrDE/rnfAE family [H]

Homologues:

Organism=Escherichia coli, GI1787919, Length=212, Percent_Identity=48.5849056603774, Blast_Score=190, Evalue=8e-50,
Organism=Escherichia coli, GI1787914, Length=160, Percent_Identity=31.875, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003667
- InterPro:   IPR010968 [H]

Pfam domain/function: PF02508 Rnf-Nqr [H]

EC number: NA

Molecular weight: Translated: 23845; Mature: 23845

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTLSEFRSLFHNGLVKQNTGLVQLLGLCPLLAISNTVVNALSLGLATMLVMAASSGAVS
CCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
AARNFVPNEIRIPVFVLIIAALVTVIDLAMNAFVHPLYLVLGIFIPLITTNCIVLARADA
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECHH
FAAKNRPLHSVVDAVAMGLGLTLVLIVLGAMRELVGQGTLFSGIDLVFGEDAKQFVLHVL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHCCCCHHHHHHHHC
PDYRGFLLAVLPPGAFIALGLLIAAHNWNKARAEQRAQLTVPATA
CCCCCEEEEEECCHHHHHHHHHHHHCCCCHHHHHHHCEEECCCCC
>Mature Secondary Structure
MMTLSEFRSLFHNGLVKQNTGLVQLLGLCPLLAISNTVVNALSLGLATMLVMAASSGAVS
CCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
AARNFVPNEIRIPVFVLIIAALVTVIDLAMNAFVHPLYLVLGIFIPLITTNCIVLARADA
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECHH
FAAKNRPLHSVVDAVAMGLGLTLVLIVLGAMRELVGQGTLFSGIDLVFGEDAKQFVLHVL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHCCCCHHHHHHHHC
PDYRGFLLAVLPPGAFIALGLLIAAHNWNKARAEQRAQLTVPATA
CCCCCEEEEEECCHHHHHHHHHHHHCCCCHHHHHHHCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA