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The map label for this gene is nth [H]

Identifier: 74318039

GI number: 74318039

Start: 2110294

End: 2110983

Strand: Direct

Name: nth [H]

Synonym: Tbd_2021

Alternate gene names: 74318039

Gene position: 2110294-2110983 (Clockwise)

Preceding gene: 74318038

Following gene: 74318040

Centisome position: 72.52

GC content: 67.68

Gene sequence:

>690_bases
ATGAACGCTGACAAACGCCGCGAGATTTTCCGCCGCCTGCGCGAAGCGAATCCCCACCCGACGACCGAGCTCGAATACGC
GACGCCTTTCGAGCTGCTGGTCGCGGTCGTGCTCTCGGCGCAGTCGACGGACAAGGGCGTCAATCGCGCGACGCGCGTGC
TCTTCCCGATCGCCAACACGCCTGCGGCGATCCACGCGCTCGGCGAGGCGGGGCTCGCCGACTACATCAAGACGATCGGA
CTCTACAAAAGCAAGGCGCGGCACCTGATCGCGGCATCGCGCATGCTGCTCGACCTGCACGGCGGCGAAGTCCCCGCCGA
CCGCGCCGCGCTCGAGGCGCTGCCCGGGGTCGGGCGCAAGACCGCCAACGTGATCCTCAACACCGCGTTCGGCCAGGCGA
CGATGGCGGTCGACACCCACATCTTCCGCGTTGCGAACCGCACCGGCCTCGCCCCCGGGAAGACCGTGCTCGAAGTCGAA
AAAAAGCTGGTCAAGACGACGCCCGCCGAGTTTCTGGTCGACGCGCACCACTGGCTGATCCTGCACGGCCGCTACGTCTG
CCAGGCGCGCAGGCCGAAGTGTGCGGAATGCATCATCGTCGACCTCTGCGAGTTCAAGGCCAAGACCCTGCCCGCTGCGC
CGCGTGCCTCCGCGCGCAAGAGGCCCGCGACCGAGGCGGCCGATGTTTAA

Upstream 100 bases:

>100_bases
GATCGCCGCGCACAACTGGAACAAGGCGCGCGCCGAGCAGCGCGCGCAGCTGACCGTTCCCGCCACGGCTTGAGGCCGGG
GCCGATGCCCGTGCCGAACG

Downstream 100 bases:

>100_bases
CCCGAGCCGCGAGCAGGTGCGCCAGTTCTTTTTCGACGTCTGGGCGAAATACCGCGCCGGACAGGCGCTCGAGGGCGCCG
AACGCACCGCGCTCGAGGGC

Product: DNA-(apurinic or apyrimidinic site) lyase / endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MNADKRREIFRRLREANPHPTTELEYATPFELLVAVVLSAQSTDKGVNRATRVLFPIANTPAAIHALGEAGLADYIKTIG
LYKSKARHLIAASRMLLDLHGGEVPADRAALEALPGVGRKTANVILNTAFGQATMAVDTHIFRVANRTGLAPGKTVLEVE
KKLVKTTPAEFLVDAHHWLILHGRYVCQARRPKCAECIIVDLCEFKAKTLPAAPRASARKRPATEAADV

Sequences:

>Translated_229_residues
MNADKRREIFRRLREANPHPTTELEYATPFELLVAVVLSAQSTDKGVNRATRVLFPIANTPAAIHALGEAGLADYIKTIG
LYKSKARHLIAASRMLLDLHGGEVPADRAALEALPGVGRKTANVILNTAFGQATMAVDTHIFRVANRTGLAPGKTVLEVE
KKLVKTTPAEFLVDAHHWLILHGRYVCQARRPKCAECIIVDLCEFKAKTLPAAPRASARKRPATEAADV
>Mature_229_residues
MNADKRREIFRRLREANPHPTTELEYATPFELLVAVVLSAQSTDKGVNRATRVLFPIANTPAAIHALGEAGLADYIKTIG
LYKSKARHLIAASRMLLDLHGGEVPADRAALEALPGVGRKTANVILNTAFGQATMAVDTHIFRVANRTGLAPGKTVLEVE
KKLVKTTPAEFLVDAHHWLILHGRYVCQARRPKCAECIIVDLCEFKAKTLPAAPRASARKRPATEAADV

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=31.0734463276836, Blast_Score=88, Evalue=6e-18,
Organism=Escherichia coli, GI1787920, Length=208, Percent_Identity=68.75, Blast_Score=304, Evalue=3e-84,
Organism=Escherichia coli, GI1789331, Length=129, Percent_Identity=34.1085271317829, Blast_Score=65, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17554540, Length=177, Percent_Identity=33.8983050847458, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6324530, Length=194, Percent_Identity=25.7731958762887, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI45550361, Length=178, Percent_Identity=31.4606741573034, Blast_Score=100, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24956; Mature: 24956

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNADKRREIFRRLREANPHPTTELEYATPFELLVAVVLSAQSTDKGVNRATRVLFPIANT
CCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHEEEECCCC
PAAIHALGEAGLADYIKTIGLYKSKARHLIAASRMLLDLHGGEVPADRAALEALPGVGRK
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHCCCCCCH
TANVILNTAFGQATMAVDTHIFRVANRTGLAPGKTVLEVEKKLVKTTPAEFLVDAHHWLI
HHHHEEECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCEEE
LHGRYVCQARRPKCAECIIVDLCEFKAKTLPAAPRASARKRPATEAADV
EECHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNADKRREIFRRLREANPHPTTELEYATPFELLVAVVLSAQSTDKGVNRATRVLFPIANT
CCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHEEEECCCC
PAAIHALGEAGLADYIKTIGLYKSKARHLIAASRMLLDLHGGEVPADRAALEALPGVGRK
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHCCCCCCH
TANVILNTAFGQATMAVDTHIFRVANRTGLAPGKTVLEVEKKLVKTTPAEFLVDAHHWLI
HHHHEEECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCEEE
LHGRYVCQARRPKCAECIIVDLCEFKAKTLPAAPRASARKRPATEAADV
EECHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]