Click here to switch to the map view.

The map label for this gene is leuA

Identifier: 74318004

GI number: 74318004

Start: 2076228

End: 2077763

Strand: Reverse

Name: leuA

Synonym: Tbd_1986

Alternate gene names: 74318004

Gene position: 2077763-2076228 (Counterclockwise)

Preceding gene: 74318005

Following gene: 74318003

Centisome position: 71.41

GC content: 65.56

Gene sequence:

>1536_bases
ATGAACGACCGCTTGTTTATTTTCGACACGACCTTGCGCGACGGGGAGCAGAGCCCGGGCGCCTCCATGACCAAGGACGA
GAAGCTGCGCATCGCGCGTCAGCTCGAGAAGCTCGGCGTCGACGTCATCGAGGCGGGTTTCGCGGCTGCGAGTCCCGGCG
ACGCCGACGCAATCCGTGCGATCGCCGAGACGGTCCGCAATTCCACGGTCTGTTCGCTCGCGCGCGCCAACGAGCGCGAC
ATCCATGCCGCTGGCGGCGCGATCAAGCCGGCAAAGTCCGGGCGCATCCATACCTTCATCGCGACCAGCCCGATCCACAT
GGAAAAGAAGCTGCGCATGCAGCCCGACCAGGTCGTCGAGGCGGCGGTCAAAGCGGTCAAGCTCGCGCGCGAATACACCG
ACGACGTCGAATTCTCGGCCGAAGACGCGGTGCGCTCCGACATGGATTTCCTCGTGCGGATCTTCGATGAAGTGATCAAG
GCGGGCGCGAAGACGATCAACGTGCCCGACACGGTCGGCTACTCGATTCCCGCGCTGTGGGGCGAGCGCATGAAAGCGCT
CATCGAGCGCGTGCCGGGGTCCGACAAGGTGATCTGGTCGACGCACTGCCACAACGACCTCGGCATGGCGGTCGCCAACT
CGCTCGCCGCCGTCATGAACGGCGCGCGCCAGGTCGAGTGCACGATCAACGGCCTTGGCGAGCGCGCCGGCAACGCGTCG
CTGGAAGAGATCGTGATGGCGGTGCGCACGCGTCGCGACGTGTTCGGCTGCGACACCCGCATCGACGCCACGCAGATCGT
CCCGTCGTCGAAACTCGTCTCGACCATCACCGGCTATCCGGTGCAGCCGAACAAGGCCATCGTCGGCGCCAACGCGTTCG
CCCACGAGTCGGGCATTCACCAGGACGGCGTTTTGAAGCACCGCGAAACCTACGAGATCATGCGCGCCGAGGACGTCGGC
TGGAACGCGAACCGCCTCACGCTCGGCAAGCTCTCCGGCCGCAACGCCTTCAAGACCAAGCTCGCCGAGCTCGGCATCGT
GCTAGACAGCGAGGAGGCACTCAACGCCGCGTTCGCGCGCTTCAAGGACCTCGCCGACAAGAAGCGCGAAATCTTCGACG
AGGATCTGCAGGCGCTCGTCTCCGACGAAGGCTACGCGACCGAGGACGAGCGCTTCAAGCTCCTGTCGATGAAGGTGTGT
TCGGAGACCGGCGAAGTGCCGCGCGCTCAGCTCGTGTTCACCGACGACGGCGCCGAAAAGCAGGCCAGCGGCGAGGGCTC
GGGCCCGGTCGACGCGACCTTCAAGGCGCTCGAATCGGTCGTGCACAGCGGCGCCGATCTCTTGCTCTATTCGGTCAACA
ACATCACCAGCGGCACCGATGCGCAGGGCGAGGTGACCGTCCGGCTCGCGCAGGATGGCCGCGTGGTCAACGGACAGGGT
GCCGACACCGACATCGTCGTCGCCTCGGCGAAGGCCTATCTGCATGCGCTCAACAAGCTGCACAGCAAACGCGAGCGCGC
GCATCCCCAGGTGTGA

Upstream 100 bases:

>100_bases
CCAGAGCAGTACCACGCGCCGCGAGTTGCATGCGGCAGCCCTATATAATGCGTCGGCATTTCGCGACCGCTCGCGACGCC
GACCCACCATGGAGTCCACC

Downstream 100 bases:

>100_bases
GGCGGCTCCGTGAACTCGACTCGGGGGCGTTTCGCTTGATCCGGCTGGCTCCCCCGGGTAGGCTTCCGGCCATGACCACG
CAATGCTTCACGACTTCTAC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MNDRLFIFDTTLRDGEQSPGASMTKDEKLRIARQLEKLGVDVIEAGFAAASPGDADAIRAIAETVRNSTVCSLARANERD
IHAAGGAIKPAKSGRIHTFIATSPIHMEKKLRMQPDQVVEAAVKAVKLAREYTDDVEFSAEDAVRSDMDFLVRIFDEVIK
AGAKTINVPDTVGYSIPALWGERMKALIERVPGSDKVIWSTHCHNDLGMAVANSLAAVMNGARQVECTINGLGERAGNAS
LEEIVMAVRTRRDVFGCDTRIDATQIVPSSKLVSTITGYPVQPNKAIVGANAFAHESGIHQDGVLKHRETYEIMRAEDVG
WNANRLTLGKLSGRNAFKTKLAELGIVLDSEEALNAAFARFKDLADKKREIFDEDLQALVSDEGYATEDERFKLLSMKVC
SETGEVPRAQLVFTDDGAEKQASGEGSGPVDATFKALESVVHSGADLLLYSVNNITSGTDAQGEVTVRLAQDGRVVNGQG
ADTDIVVASAKAYLHALNKLHSKRERAHPQV

Sequences:

>Translated_511_residues
MNDRLFIFDTTLRDGEQSPGASMTKDEKLRIARQLEKLGVDVIEAGFAAASPGDADAIRAIAETVRNSTVCSLARANERD
IHAAGGAIKPAKSGRIHTFIATSPIHMEKKLRMQPDQVVEAAVKAVKLAREYTDDVEFSAEDAVRSDMDFLVRIFDEVIK
AGAKTINVPDTVGYSIPALWGERMKALIERVPGSDKVIWSTHCHNDLGMAVANSLAAVMNGARQVECTINGLGERAGNAS
LEEIVMAVRTRRDVFGCDTRIDATQIVPSSKLVSTITGYPVQPNKAIVGANAFAHESGIHQDGVLKHRETYEIMRAEDVG
WNANRLTLGKLSGRNAFKTKLAELGIVLDSEEALNAAFARFKDLADKKREIFDEDLQALVSDEGYATEDERFKLLSMKVC
SETGEVPRAQLVFTDDGAEKQASGEGSGPVDATFKALESVVHSGADLLLYSVNNITSGTDAQGEVTVRLAQDGRVVNGQG
ADTDIVVASAKAYLHALNKLHSKRERAHPQV
>Mature_511_residues
MNDRLFIFDTTLRDGEQSPGASMTKDEKLRIARQLEKLGVDVIEAGFAAASPGDADAIRAIAETVRNSTVCSLARANERD
IHAAGGAIKPAKSGRIHTFIATSPIHMEKKLRMQPDQVVEAAVKAVKLAREYTDDVEFSAEDAVRSDMDFLVRIFDEVIK
AGAKTINVPDTVGYSIPALWGERMKALIERVPGSDKVIWSTHCHNDLGMAVANSLAAVMNGARQVECTINGLGERAGNAS
LEEIVMAVRTRRDVFGCDTRIDATQIVPSSKLVSTITGYPVQPNKAIVGANAFAHESGIHQDGVLKHRETYEIMRAEDVG
WNANRLTLGKLSGRNAFKTKLAELGIVLDSEEALNAAFARFKDLADKKREIFDEDLQALVSDEGYATEDERFKLLSMKVC
SETGEVPRAQLVFTDDGAEKQASGEGSGPVDATFKALESVVHSGADLLLYSVNNITSGTDAQGEVTVRLAQDGRVVNGQG
ADTDIVVASAKAYLHALNKLHSKRERAHPQV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=500, Percent_Identity=48.4, Blast_Score=457, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6324225, Length=544, Percent_Identity=27.0220588235294, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6320071, Length=381, Percent_Identity=28.3464566929134, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324682, Length=543, Percent_Identity=26.8876611418048, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320019, Length=378, Percent_Identity=28.042328042328, Blast_Score=130, Evalue=4e-31,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_THIDA (Q3SHE7)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315744.1
- ProteinModelPortal:   Q3SHE7
- SMR:   Q3SHE7
- STRING:   Q3SHE7
- GeneID:   3671738
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_1986
- NMPDR:   fig|292415.3.peg.2102
- eggNOG:   COG0119
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- PhylomeDB:   Q3SHE7
- ProtClustDB:   PRK00915
- BioCyc:   TDEN292415:TBD_1986-MONOMER
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 55299; Mature: 55299

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDRLFIFDTTLRDGEQSPGASMTKDEKLRIARQLEKLGVDVIEAGFAAASPGDADAIRA
CCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCHHHHHH
IAETVRNSTVCSLARANERDIHAAGGAIKPAKSGRIHTFIATSPIHMEKKLRMQPDQVVE
HHHHHHCCHHHHHHHCCCCCEECCCCCCCCCCCCCEEEEEECCCHHHHHHHCCCHHHHHH
AAVKAVKLAREYTDDVEFSAEDAVRSDMDFLVRIFDEVIKAGAKTINVPDTVGYSIPALW
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHH
GERMKALIERVPGSDKVIWSTHCHNDLGMAVANSLAAVMNGARQVECTINGLGERAGNAS
HHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCC
LEEIVMAVRTRRDVFGCDTRIDATQIVPSSKLVSTITGYPVQPNKAIVGANAFAHESGIH
HHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHCCCCCCCCCEEEECCHHHHCCCCC
QDGVLKHRETYEIMRAEDVGWNANRLTLGKLSGRNAFKTKLAELGIVLDSEEALNAAFAR
CCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHCEEECCHHHHHHHHHH
FKDLADKKREIFDEDLQALVSDEGYATEDERFKLLSMKVCSETGEVPRAQLVFTDDGAEK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCC
QASGEGSGPVDATFKALESVVHSGADLLLYSVNNITSGTDAQGEVTVRLAQDGRVVNGQG
CCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEEECCCEEECCCC
ADTDIVVASAKAYLHALNKLHSKRERAHPQV
CCCEEEEEHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNDRLFIFDTTLRDGEQSPGASMTKDEKLRIARQLEKLGVDVIEAGFAAASPGDADAIRA
CCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCHHHHHH
IAETVRNSTVCSLARANERDIHAAGGAIKPAKSGRIHTFIATSPIHMEKKLRMQPDQVVE
HHHHHHCCHHHHHHHCCCCCEECCCCCCCCCCCCCEEEEEECCCHHHHHHHCCCHHHHHH
AAVKAVKLAREYTDDVEFSAEDAVRSDMDFLVRIFDEVIKAGAKTINVPDTVGYSIPALW
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHH
GERMKALIERVPGSDKVIWSTHCHNDLGMAVANSLAAVMNGARQVECTINGLGERAGNAS
HHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCC
LEEIVMAVRTRRDVFGCDTRIDATQIVPSSKLVSTITGYPVQPNKAIVGANAFAHESGIH
HHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHCCCCCCCCCEEEECCHHHHCCCCC
QDGVLKHRETYEIMRAEDVGWNANRLTLGKLSGRNAFKTKLAELGIVLDSEEALNAAFAR
CCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHCEEECCHHHHHHHHHH
FKDLADKKREIFDEDLQALVSDEGYATEDERFKLLSMKVCSETGEVPRAQLVFTDDGAEK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCC
QASGEGSGPVDATFKALESVVHSGADLLLYSVNNITSGTDAQGEVTVRLAQDGRVVNGQG
CCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEEECCCEEECCCC
ADTDIVVASAKAYLHALNKLHSKRERAHPQV
CCCEEEEEHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA