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The map label for this gene is purU [H]

Identifier: 74317995

GI number: 74317995

Start: 2069406

End: 2070260

Strand: Direct

Name: purU [H]

Synonym: Tbd_1977

Alternate gene names: 74317995

Gene position: 2069406-2070260 (Clockwise)

Preceding gene: 74317989

Following gene: 74317996

Centisome position: 71.12

GC content: 63.63

Gene sequence:

>855_bases
ATGCGCCACACCGCCGTCCTGCTGATTTCCTGCCCCGACCAGCGCGGCCTGGTCGCGGCCATCGCCGACTTTCTGCTCGC
CCACCGCGCCAACATCCTGCACGCCGACCAGCACCAGGACGCGGAACTCAAGCTGTTCCTTATGCGGGTGGAATGGGACC
TGGCCGGCTTCAACCTCGACCTGCGCGACTTCGCCACGGCCTTCCAGCCCATCGCCGAGCGCTTCGGCATGACCTGGCGG
CTCGCCGAATCGAGCCGCAAGCCGCGCATGGCGGTGTTCGTGTCGAAGTTCGACCATTGCCTCGCCGACCTGCTCTACCG
CTACCAGAGCGGCGAACTGCACTGCGAACTACCGATCATCCTGTCCAACCATGAAGACACGCGCTGGCTGGCCGACGCCT
ACCGCGTGCCCTACCAGCACATGGCGGTAACAAAGGAAAGCAAGCACGAGACCGAGCAGATCCAGCTGGCGATCCTTCGC
GATCAGAAGATCGACTTCATCGTGCTCGCGCGCTACATGCAGGTGCTGTCGGGTGACTTCATCCGCCATTTCCCCAACCG
CATCATCAACATCCACCATTCCTTCCTGCCGGCATTTCACGGCGCCAAGCCCTACCACCGCGCTTTCGAGCGCGGCGTCA
AGCTGATCGGCGCGACCGCGCACTACGTCACCGAGACGCTCGACGACGGCCCGATCATCGAGCAGGACGTCGCGCGCATC
TCGCACCGCGACCACATCGACGACCTCATTCACAAGGGGGCGGACCTCGAGAAGGTCGTGCTGTCGCGTGCCGTGAAATG
GCATCTCGACAACCGGGTGCTGGTCTACGCGAACAAGACCGTCGTCTTCGACTGA

Upstream 100 bases:

>100_bases
CTGCTGAATCTTTACGCGGCCGCGGTTCGCGCCCCGTAAAGGGCGGACCGAATATTGGTTATTATTGCGGCAAAAACCAC
AACGGGAACGTTCCTCCGCC

Downstream 100 bases:

>100_bases
GAATGGCCGGCCCCGAGCACACACCGCCCGGCGCGGCGCTGCAGGCCGACGTCGAGACGCGGCCGAAGGCGGTCGCGGCC
TGGCTCGAGCGCCTGCCTTA

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MRHTAVLLISCPDQRGLVAAIADFLLAHRANILHADQHQDAELKLFLMRVEWDLAGFNLDLRDFATAFQPIAERFGMTWR
LAESSRKPRMAVFVSKFDHCLADLLYRYQSGELHCELPIILSNHEDTRWLADAYRVPYQHMAVTKESKHETEQIQLAILR
DQKIDFIVLARYMQVLSGDFIRHFPNRIINIHHSFLPAFHGAKPYHRAFERGVKLIGATAHYVTETLDDGPIIEQDVARI
SHRDHIDDLIHKGADLEKVVLSRAVKWHLDNRVLVYANKTVVFD

Sequences:

>Translated_284_residues
MRHTAVLLISCPDQRGLVAAIADFLLAHRANILHADQHQDAELKLFLMRVEWDLAGFNLDLRDFATAFQPIAERFGMTWR
LAESSRKPRMAVFVSKFDHCLADLLYRYQSGELHCELPIILSNHEDTRWLADAYRVPYQHMAVTKESKHETEQIQLAILR
DQKIDFIVLARYMQVLSGDFIRHFPNRIINIHHSFLPAFHGAKPYHRAFERGVKLIGATAHYVTETLDDGPIIEQDVARI
SHRDHIDDLIHKGADLEKVVLSRAVKWHLDNRVLVYANKTVVFD
>Mature_284_residues
MRHTAVLLISCPDQRGLVAAIADFLLAHRANILHADQHQDAELKLFLMRVEWDLAGFNLDLRDFATAFQPIAERFGMTWR
LAESSRKPRMAVFVSKFDHCLADLLYRYQSGELHCELPIILSNHEDTRWLADAYRVPYQHMAVTKESKHETEQIQLAILR
DQKIDFIVLARYMQVLSGDFIRHFPNRIINIHHSFLPAFHGAKPYHRAFERGVKLIGATAHYVTETLDDGPIIEQDVARI
SHRDHIDDLIHKGADLEKVVLSRAVKWHLDNRVLVYANKTVVFD

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=176, Percent_Identity=28.4090909090909, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI209869995, Length=176, Percent_Identity=28.4090909090909, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI209869993, Length=176, Percent_Identity=28.4090909090909, Blast_Score=82, Evalue=4e-16,
Organism=Escherichia coli, GI1787483, Length=278, Percent_Identity=41.3669064748201, Blast_Score=216, Evalue=9e-58,
Organism=Escherichia coli, GI1788846, Length=114, Percent_Identity=33.3333333333333, Blast_Score=78, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17567511, Length=170, Percent_Identity=34.1176470588235, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24582400, Length=197, Percent_Identity=28.9340101522843, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32858; Mature: 32858

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHTAVLLISCPDQRGLVAAIADFLLAHRANILHADQHQDAELKLFLMRVEWDLAGFNLD
CCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCEEEEEEEEEEEECCCCCC
LRDFATAFQPIAERFGMTWRLAESSRKPRMAVFVSKFDHCLADLLYRYQSGELHCELPII
HHHHHHHHHHHHHHHCCEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
LSNHEDTRWLADAYRVPYQHMAVTKESKHETEQIQLAILRDQKIDFIVLARYMQVLSGDF
EECCCCHHHHHHHHHCCHHHHHHCCHHCCHHHHEEEEEECCCCCCHHHHHHHHHHHCCHH
IRHFPNRIINIHHSFLPAFHGAKPYHRAFERGVKLIGATAHYVTETLDDGPIIEQDVARI
HHHCCHHHEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
SHRDHIDDLIHKGADLEKVVLSRAVKWHLDNRVLVYANKTVVFD
HHHHHHHHHHHCCCCHHHHHHHHHHHEECCCEEEEEECCEEEEC
>Mature Secondary Structure
MRHTAVLLISCPDQRGLVAAIADFLLAHRANILHADQHQDAELKLFLMRVEWDLAGFNLD
CCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCEEEEEEEEEEEECCCCCC
LRDFATAFQPIAERFGMTWRLAESSRKPRMAVFVSKFDHCLADLLYRYQSGELHCELPII
HHHHHHHHHHHHHHHCCEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
LSNHEDTRWLADAYRVPYQHMAVTKESKHETEQIQLAILRDQKIDFIVLARYMQVLSGDF
EECCCCHHHHHHHHHCCHHHHHHCCHHCCHHHHEEEEEECCCCCCHHHHHHHHHHHCCHH
IRHFPNRIINIHHSFLPAFHGAKPYHRAFERGVKLIGATAHYVTETLDDGPIIEQDVARI
HHHCCHHHEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
SHRDHIDDLIHKGADLEKVVLSRAVKWHLDNRVLVYANKTVVFD
HHHHHHHHHHHCCCCHHHHHHHHHHHEECCCEEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]