Click here to switch to the map view.

The map label for this gene is leuD

Identifier: 74317939

GI number: 74317939

Start: 2017942

End: 2018580

Strand: Reverse

Name: leuD

Synonym: Tbd_1921

Alternate gene names: 74317939

Gene position: 2018580-2017942 (Counterclockwise)

Preceding gene: 74317940

Following gene: 74317938

Centisome position: 69.37

GC content: 66.67

Gene sequence:

>639_bases
ATGCAGGCATTCACGACCCTGGACGGGCTGGTCGTGCCGCTCGACCGCGCCAACGTCGACACCGACGCGATCATTCCCAA
GCAGTTCCTGAAGTCGATCAAGCGCGCGGGCTTCGGCCCCAACCTGTTCGACGAATGGCGCTACCTCGATCACGGCGAGC
CCGGCATGGACCCGGCGACGCGCCAGCCCAATCCGGCGTTCGTGCTCAATCTCCCGCGCTACGCCGGTGCCTCGGTGCTG
CTCGCGCGCGACAACTTCGGCTGCGGCTCGAGCCGCGAGCACGCGCCATGGGCGCTCGAGGACTACGGCATCCGCGCGAT
CATCGCACCGAGTTTTGCCGACATCTTTTACAACAACTGCTTCAAGAACGGCATCCTGCCGATCGTGCTCGACGCCGCCG
CAGTCGACCGCCTGTTCGGCGAATGCGAGGCGAGCGCGGGCTACCGCCTCAAGATCGACCTCGAGCAGCAGACCGTGACG
ACGCCGGGCGGCGAGGTGCTGCGCTTCGAGGTCGATGCCGGGCGCAAGCACCGGCTGCTCAACGGCCTCGACGACATCGG
CCTGACGCTTCTGCAGGCCGACAAGATCAAGGCCTACGAAGCGCGGCGCAGGCAGGAAGCGCCGTGGTTGTTTGCGTGA

Upstream 100 bases:

>100_bases
TGATTCTTGGGGGCTGCAACACCATGGAAGGCTTTGGTCAGGACGTCCAGAAGGCGGGCAGCGCCATCGAGAAGGCCGGC
AGCAAGGCCGGTAACTAGCC

Downstream 100 bases:

>100_bases
ATTTTTCATCCGTGAATGCCCGAAGGGAATTCGCGGAAAAAGGGTTTTGAGAACATGAAGATTGCAGTTTTGCCGGGTGA
CGGCATCGGTCCGGAAATCA

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MQAFTTLDGLVVPLDRANVDTDAIIPKQFLKSIKRAGFGPNLFDEWRYLDHGEPGMDPATRQPNPAFVLNLPRYAGASVL
LARDNFGCGSSREHAPWALEDYGIRAIIAPSFADIFYNNCFKNGILPIVLDAAAVDRLFGECEASAGYRLKIDLEQQTVT
TPGGEVLRFEVDAGRKHRLLNGLDDIGLTLLQADKIKAYEARRRQEAPWLFA

Sequences:

>Translated_212_residues
MQAFTTLDGLVVPLDRANVDTDAIIPKQFLKSIKRAGFGPNLFDEWRYLDHGEPGMDPATRQPNPAFVLNLPRYAGASVL
LARDNFGCGSSREHAPWALEDYGIRAIIAPSFADIFYNNCFKNGILPIVLDAAAVDRLFGECEASAGYRLKIDLEQQTVT
TPGGEVLRFEVDAGRKHRLLNGLDDIGLTLLQADKIKAYEARRRQEAPWLFA
>Mature_212_residues
MQAFTTLDGLVVPLDRANVDTDAIIPKQFLKSIKRAGFGPNLFDEWRYLDHGEPGMDPATRQPNPAFVLNLPRYAGASVL
LARDNFGCGSSREHAPWALEDYGIRAIIAPSFADIFYNNCFKNGILPIVLDAAAVDRLFGECEASAGYRLKIDLEQQTVT
TPGGEVLRFEVDAGRKHRLLNGLDDIGLTLLQADKIKAYEARRRQEAPWLFA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=202, Percent_Identity=58.9108910891089, Blast_Score=240, Evalue=5e-65,
Organism=Saccharomyces cerevisiae, GI6321429, Length=214, Percent_Identity=52.803738317757, Blast_Score=206, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_THIDA (Q3SHL2)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315679.1
- STRING:   Q3SHL2
- GeneID:   3674031
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_1921
- NMPDR:   fig|292415.3.peg.2018
- eggNOG:   COG0066
- HOGENOM:   HBG304838
- OMA:   DEISITM
- PhylomeDB:   Q3SHL2
- ProtClustDB:   PRK01641
- BioCyc:   TDEN292415:TBD_1921-MONOMER
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 23536; Mature: 23536

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAFTTLDGLVVPLDRANVDTDAIIPKQFLKSIKRAGFGPNLFDEWRYLDHGEPGMDPAT
CCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCC
RQPNPAFVLNLPRYAGASVLLARDNFGCGSSREHAPWALEDYGIRAIIAPSFADIFYNNC
CCCCCEEEEECCCCCCCEEEEEECCCCCCCCCCCCCEEHHCCCEEEEECCHHHHHHHHHH
FKNGILPIVLDAAAVDRLFGECEASAGYRLKIDLEQQTVTTPGGEVLRFEVDAGRKHRLL
HHCCCEEEEEHHHHHHHHHHHHCCCCCEEEEEEECCEEEECCCCEEEEEEECCCCHHHHH
NGLDDIGLTLLQADKIKAYEARRRQEAPWLFA
CCHHHHCEEEHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQAFTTLDGLVVPLDRANVDTDAIIPKQFLKSIKRAGFGPNLFDEWRYLDHGEPGMDPAT
CCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCC
RQPNPAFVLNLPRYAGASVLLARDNFGCGSSREHAPWALEDYGIRAIIAPSFADIFYNNC
CCCCCEEEEECCCCCCCEEEEEECCCCCCCCCCCCCEEHHCCCEEEEECCHHHHHHHHHH
FKNGILPIVLDAAAVDRLFGECEASAGYRLKIDLEQQTVTTPGGEVLRFEVDAGRKHRLL
HHCCCEEEEEHHHHHHHHHHHHCCCCCEEEEEEECCEEEECCCCEEEEEEECCCCHHHHH
NGLDDIGLTLLQADKIKAYEARRRQEAPWLFA
CCHHHHCEEEHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA