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The map label for this gene is leuC

Identifier: 74317940

GI number: 74317940

Start: 2018730

End: 2020133

Strand: Reverse

Name: leuC

Synonym: Tbd_1922

Alternate gene names: 74317940

Gene position: 2020133-2018730 (Counterclockwise)

Preceding gene: 74317941

Following gene: 74317939

Centisome position: 69.42

GC content: 69.16

Gene sequence:

>1404_bases
ATGGCTGGCCAAACCTTATTCCAGAAGCTGTGGGACGCCCACGTCGTCCATGTCGAACCCGACGGCACGACGCTGCTCTA
CATCGATCGTCACCTCGTGCACGAGGTGACGAGTCCGCAGGCCTTCGAGGGGCTCAAGCTCGCGCAGCGCCTGCCCCGGC
GGCCCGACGCGGCGATCGCCGTGCCCGACCACAACGTTCCGACGACCGATCGCTCACAGGGCATCGCCGACCCGGTGTCG
CGCCTGCAGGTCGAAACGCTCGACGCCAACTGCGCCGAGTTCGGCATCACCGAATTCCGGATGGACGACGTCCGCCAGGG
CATCGTCCACGTCATCGGCCCCGAGGAGGGCCTGACCCTGCCGGGCATGACCGTGGTGTGCGGTGATTCGCATACCTCGA
CGCACGGGGCGTTCGGGGCATTGGCGTTCGGCATTGGCACGTCCGAGGTCGAGCACGTGCTGGCGACGCAATGCCTGTGG
CAGAAGCCGTCGAAAACGATGCTGGTCAAGGTCGAGGGCGAATTGGGCAAGGGCGTGACGGCCAAGGACATCGCGCTCGC
CGTCATCGGCCGCATCGGCACGGCCGGCGGCACCGGCTACGCGATCGAGTTCGGCGGCGCGGCGATCCGCGCGCTGTCGA
TGGAAGGCCGCATGACGCTGTGCAACATGGCGATCGAAGCGGGCGCGCGCGCCGGCATGGTTGCGGTCGATCAGACGACG
ATCGACTACGTCGAAGGGCGTCCCTATGCGCCGACGGGCGAGGCCTGGGACACGGCGGTGGCGTGGTGGAAAACCCTGCA
CTCGGACGCGGACGCCGTGTTCGACCGCGTGGTCGAACTCGACGCCGCGGCCATCGAGCCGCAGGTGACCTGGGGAACGT
CGCCCGAGATGGTGACCACGGTGGGCGCGAGCGTGCCCGACCCGAGTGCGGCGCCGAGCGAGGTCAAGCGTCAGGACTGG
GTGCGCGCACTCGAATATATGGGGCTCGCGGCCGGCACGCCGGTCAGCGCGATCGCGCTCGACAAGGTTTTCATCGGCTC
GTGCACCAACTCGCGGATCGAGGACCTGCGCGAGGCTGCGGCCGTCGCCAAGGGGCGCCGGGTCGCGCCCAACGTGAAGC
TCGCGATGGTCGTGCCGGGCTCCGGGCTGGTCAAGCAGCAGGCCGAGGCGGAGGGCCTCGACCGGATCTTCATCGACGCC
GGATTCGAGTGGCGCGAGCCGGGCTGCTCGATGTGCCTCGCAATGAACGCCGACCGGCTCGAGCCCGGCGAGCGCTGCGC
GTCCACCTCCAATCGCAACTTCGAAGGCCGCCAGGGGCAGGGCGGGCGCACCCATCTCGTGAGTCCGGCGATGGCCGCCG
CCGCGGCGTGCGCCGGCCACTTCGTCGACGTCCGCAACTTTTAA

Upstream 100 bases:

>100_bases
GGACAAACAAAACCCGTGCCAGCGCAGGCGCGATAAATGATTCATCCCGCTCAAGCTATGAAATAATCGCTGTTTTGCAC
GCTTCATCGCCCGATTCCGC

Downstream 100 bases:

>100_bases
GGAGAACGTATGAAACCGATGATCGTCGTGCTGGCTGCCGCGGCTGCGCTGATTCTTGGGGGCTGCAACACCATGGAAGG
CTTTGGTCAGGACGTCCAGA

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MAGQTLFQKLWDAHVVHVEPDGTTLLYIDRHLVHEVTSPQAFEGLKLAQRLPRRPDAAIAVPDHNVPTTDRSQGIADPVS
RLQVETLDANCAEFGITEFRMDDVRQGIVHVIGPEEGLTLPGMTVVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLW
QKPSKTMLVKVEGELGKGVTAKDIALAVIGRIGTAGGTGYAIEFGGAAIRALSMEGRMTLCNMAIEAGARAGMVAVDQTT
IDYVEGRPYAPTGEAWDTAVAWWKTLHSDADAVFDRVVELDAAAIEPQVTWGTSPEMVTTVGASVPDPSAAPSEVKRQDW
VRALEYMGLAAGTPVSAIALDKVFIGSCTNSRIEDLREAAAVAKGRRVAPNVKLAMVVPGSGLVKQQAEAEGLDRIFIDA
GFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGQGGRTHLVSPAMAAAAACAGHFVDVRNF

Sequences:

>Translated_467_residues
MAGQTLFQKLWDAHVVHVEPDGTTLLYIDRHLVHEVTSPQAFEGLKLAQRLPRRPDAAIAVPDHNVPTTDRSQGIADPVS
RLQVETLDANCAEFGITEFRMDDVRQGIVHVIGPEEGLTLPGMTVVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLW
QKPSKTMLVKVEGELGKGVTAKDIALAVIGRIGTAGGTGYAIEFGGAAIRALSMEGRMTLCNMAIEAGARAGMVAVDQTT
IDYVEGRPYAPTGEAWDTAVAWWKTLHSDADAVFDRVVELDAAAIEPQVTWGTSPEMVTTVGASVPDPSAAPSEVKRQDW
VRALEYMGLAAGTPVSAIALDKVFIGSCTNSRIEDLREAAAVAKGRRVAPNVKLAMVVPGSGLVKQQAEAEGLDRIFIDA
GFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGQGGRTHLVSPAMAAAAACAGHFVDVRNF
>Mature_466_residues
AGQTLFQKLWDAHVVHVEPDGTTLLYIDRHLVHEVTSPQAFEGLKLAQRLPRRPDAAIAVPDHNVPTTDRSQGIADPVSR
LQVETLDANCAEFGITEFRMDDVRQGIVHVIGPEEGLTLPGMTVVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLWQ
KPSKTMLVKVEGELGKGVTAKDIALAVIGRIGTAGGTGYAIEFGGAAIRALSMEGRMTLCNMAIEAGARAGMVAVDQTTI
DYVEGRPYAPTGEAWDTAVAWWKTLHSDADAVFDRVVELDAAAIEPQVTWGTSPEMVTTVGASVPDPSAAPSEVKRQDWV
RALEYMGLAAGTPVSAIALDKVFIGSCTNSRIEDLREAAAVAKGRRVAPNVKLAMVVPGSGLVKQQAEAEGLDRIFIDAG
FEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGQGGRTHLVSPAMAAAAACAGHFVDVRNF

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=365, Percent_Identity=27.3972602739726, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI41352693, Length=385, Percent_Identity=26.4935064935065, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI8659555, Length=401, Percent_Identity=26.9326683291771, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1786259, Length=464, Percent_Identity=64.8706896551724, Blast_Score=610, Evalue=1e-176,
Organism=Escherichia coli, GI1787531, Length=368, Percent_Identity=28.804347826087, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25149337, Length=365, Percent_Identity=27.6712328767123, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI32564738, Length=365, Percent_Identity=27.6712328767123, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI25149342, Length=313, Percent_Identity=26.8370607028754, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17568399, Length=394, Percent_Identity=26.9035532994924, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321429, Length=470, Percent_Identity=63.4042553191489, Blast_Score=598, Evalue=1e-172,
Organism=Saccharomyces cerevisiae, GI6320440, Length=465, Percent_Identity=26.8817204301075, Blast_Score=143, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6323335, Length=365, Percent_Identity=30.1369863013699, Blast_Score=133, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6322261, Length=365, Percent_Identity=29.5890410958904, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI281365315, Length=397, Percent_Identity=25.9445843828715, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17864292, Length=397, Percent_Identity=25.9445843828715, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI161076999, Length=397, Percent_Identity=25.9445843828715, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI28571643, Length=406, Percent_Identity=25.3694581280788, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI17137564, Length=384, Percent_Identity=26.3020833333333, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24645686, Length=385, Percent_Identity=28.3116883116883, Blast_Score=86, Evalue=6e-17,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_THIDA (Q3SHL1)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315680.1
- ProteinModelPortal:   Q3SHL1
- SMR:   Q3SHL1
- STRING:   Q3SHL1
- GeneID:   3674032
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_1922
- NMPDR:   fig|292415.3.peg.2019
- eggNOG:   COG0065
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- PhylomeDB:   Q3SHL1
- ProtClustDB:   PRK05478
- BioCyc:   TDEN292415:TBD_1922-MONOMER
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 49668; Mature: 49537

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGQTLFQKLWDAHVVHVEPDGTTLLYIDRHLVHEVTSPQAFEGLKLAQRLPRRPDAAIA
CCCHHHHHHHCCCEEEEECCCCCEEEEECHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEE
VPDHNVPTTDRSQGIADPVSRLQVETLDANCAEFGITEFRMDDVRQGIVHVIGPEEGLTL
CCCCCCCCCCCCCCCHHHHHHEEEEECCCCHHHHCCHHHHHHHHHCCEEEEECCCCCCCC
PGMTVVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLWQKPSKTMLVKVEGELGKGVT
CCEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCC
AKDIALAVIGRIGTAGGTGYAIEFGGAAIRALSMEGRMTLCNMAIEAGARAGMVAVDQTT
HHHHHHHHHHHCCCCCCCEEEEEECCEEEEEEECCCCHHHHHHHHHCCCCCCEEEEECCH
IDYVEGRPYAPTGEAWDTAVAWWKTLHSDADAVFDRVVELDAAAIEPQVTWGTSPEMVTT
HHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEECCCCCCCEEE
VGASVPDPSAAPSEVKRQDWVRALEYMGLAAGTPVSAIALDKVFIGSCTNSRIEDLREAA
ECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHEECCCCCHHHHHHHHHH
AVAKGRRVAPNVKLAMVVPGSGLVKQQAEAEGLDRIFIDAGFEWREPGCSMCLAMNADRL
HHHCCCCCCCCCEEEEEECCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEECCCCCC
EPGERCASTSNRNFEGRQGQGGRTHLVSPAMAAAAACAGHFVDVRNF
CCHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHCEEECCC
>Mature Secondary Structure 
AGQTLFQKLWDAHVVHVEPDGTTLLYIDRHLVHEVTSPQAFEGLKLAQRLPRRPDAAIA
CCHHHHHHHCCCEEEEECCCCCEEEEECHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEE
VPDHNVPTTDRSQGIADPVSRLQVETLDANCAEFGITEFRMDDVRQGIVHVIGPEEGLTL
CCCCCCCCCCCCCCCHHHHHHEEEEECCCCHHHHCCHHHHHHHHHCCEEEEECCCCCCCC
PGMTVVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLWQKPSKTMLVKVEGELGKGVT
CCEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCC
AKDIALAVIGRIGTAGGTGYAIEFGGAAIRALSMEGRMTLCNMAIEAGARAGMVAVDQTT
HHHHHHHHHHHCCCCCCCEEEEEECCEEEEEEECCCCHHHHHHHHHCCCCCCEEEEECCH
IDYVEGRPYAPTGEAWDTAVAWWKTLHSDADAVFDRVVELDAAAIEPQVTWGTSPEMVTT
HHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEECCCCCCCEEE
VGASVPDPSAAPSEVKRQDWVRALEYMGLAAGTPVSAIALDKVFIGSCTNSRIEDLREAA
ECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHEECCCCCHHHHHHHHHH
AVAKGRRVAPNVKLAMVVPGSGLVKQQAEAEGLDRIFIDAGFEWREPGCSMCLAMNADRL
HHHCCCCCCCCCEEEEEECCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEECCCCCC
EPGERCASTSNRNFEGRQGQGGRTHLVSPAMAAAAACAGHFVDVRNF
CCHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA