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The map label for this gene is folC [H]

Identifier: 74317929

GI number: 74317929

Start: 2006768

End: 2008036

Strand: Reverse

Name: folC [H]

Synonym: Tbd_1911

Alternate gene names: 74317929

Gene position: 2008036-2006768 (Counterclockwise)

Preceding gene: 74317930

Following gene: 74317928

Centisome position: 69.01

GC content: 69.98

Gene sequence:

>1269_bases
TTGCCGCCTAGCGCATCGCTCGCCGCTTGGCTCGCCCGGCTCGAGACGCTGCATCCCAGCGCCATCGAACTGGGCCTCGA
ACGCGTCCGACGCGTCAAGGACGCGATGGGATTGGCACCCGACTTCCCGCTCATCGTCGTCGGCGGCACCAACGGCAAGG
GCTCGACCTGCGCCTATCTCGAGGCGGTTTTCGGCGCCGCCGGCTACAAGACCGGTCTCTATACCTCGCCCCACCTGCTG
CGCTACAACGAGCGCGTCCGCATCGCGGGCAAGGAGGTCGACGACGCCGATCTGGTCGCCGCGTTTGAGGCCGTCGACCG
GGCGCGCGGCGACGTCGCGCTGACCTATTTCGAATTCGGCACGCTTGCCGCTATGGCACAGTTCGTCGCCGCCAAGGTCG
ACGTCGCGATCCTCGAAGTCGGCCTTGGTGGACGCCTCGACGCGGTCAACGTGTTCGACGCCGACGCCGCGATCGTGACC
AGCGTCGACCTCGACCACATGGATTACCTCGGCGACACGCGCGAACAAATCGGATTCGAGAAGGCCGGTATCTACCGGGC
CGGCCGGCCCGCGGTCTGCGCCGACCCCGAACCGCCTGCCAGCCTGCTTGCCCACGCCGGGAAAGTCGGCGCCGATCTGC
GCCGGCTCGGCCGCGATTTCTCCGCCGCGCGTGCCGGGGAGCGCTGGACCTATCACAGCCGCGCGCTCAGTTGGCCGGAT
CTGCCCTTGCCGGCCATGGCCGGGCCCTGCCAGTTGCGTAACGCCGCCGGTGCGCTCGCCGTCCTCGAGGCGCTACAGGC
CCGGTTGCCGACCTCGTCCGAAGCGATCCGCCGAGGCGTCGCGGAAGCGCGCGTGGCCGGGCGCTTCCAGCGCATCGGGC
GCAGGCCCGAGGTGATCGTCGACGTCGCACATAACCCCGAGGCGGCGCGTGCGCTGGCGTCGACGCTGCGCGAGCAGCCG
ATCTCCGGGCGCACGCTCGCCGTCGTCGGAATGCTCGCCGACAAGGATGCCGGAGCGGTCGTCGCGGCGCTGGCCGACGG
CGTCGACGCGTGGTGGACATGCACACCTGATTCCCCGCGGGCCCGCGACGCCAGCTCGCTGGCCGCGGTCCTGCACGCGC
ACGGCGTGCGTAGCGTGAATGTGCAGCCGGGCGTGAAGGCGGCGCTTGCGGCGGCGCGAAGTGCAGCCGGTGAAGATGAT
AGAATCCTCGTCTTCGGTTCGTTCTACACCGTCGCTGCAGTCCTCGATCATGCCGCCACCCAGCAGTAG

Upstream 100 bases:

>100_bases
TTCCTGCAGGAGAAGGGCGCGGTCGATCGCATCATTGACCGCCGCCAGATGCGCGACGACCTGGCTGCCACGCTTGCGAT
CATGATGGGACGCCCGGCGC

Downstream 100 bases:

>100_bases
TGAAAATGAGCTCAGGCGCCGCGCCCGCCGGCGGCTGATCGGCGCGGTCGCGCTGACGCTCGTCGCGGTCATCGCGCTGC
CGCTGCTGCTCGAAGACGAA

Product: folylpolyglutamate synthetase

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase [H]

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MPPSASLAAWLARLETLHPSAIELGLERVRRVKDAMGLAPDFPLIVVGGTNGKGSTCAYLEAVFGAAGYKTGLYTSPHLL
RYNERVRIAGKEVDDADLVAAFEAVDRARGDVALTYFEFGTLAAMAQFVAAKVDVAILEVGLGGRLDAVNVFDADAAIVT
SVDLDHMDYLGDTREQIGFEKAGIYRAGRPAVCADPEPPASLLAHAGKVGADLRRLGRDFSAARAGERWTYHSRALSWPD
LPLPAMAGPCQLRNAAGALAVLEALQARLPTSSEAIRRGVAEARVAGRFQRIGRRPEVIVDVAHNPEAARALASTLREQP
ISGRTLAVVGMLADKDAGAVVAALADGVDAWWTCTPDSPRARDASSLAAVLHAHGVRSVNVQPGVKAALAAARSAAGEDD
RILVFGSFYTVAAVLDHAATQQ

Sequences:

>Translated_422_residues
MPPSASLAAWLARLETLHPSAIELGLERVRRVKDAMGLAPDFPLIVVGGTNGKGSTCAYLEAVFGAAGYKTGLYTSPHLL
RYNERVRIAGKEVDDADLVAAFEAVDRARGDVALTYFEFGTLAAMAQFVAAKVDVAILEVGLGGRLDAVNVFDADAAIVT
SVDLDHMDYLGDTREQIGFEKAGIYRAGRPAVCADPEPPASLLAHAGKVGADLRRLGRDFSAARAGERWTYHSRALSWPD
LPLPAMAGPCQLRNAAGALAVLEALQARLPTSSEAIRRGVAEARVAGRFQRIGRRPEVIVDVAHNPEAARALASTLREQP
ISGRTLAVVGMLADKDAGAVVAALADGVDAWWTCTPDSPRARDASSLAAVLHAHGVRSVNVQPGVKAALAAARSAAGEDD
RILVFGSFYTVAAVLDHAATQQ
>Mature_421_residues
PPSASLAAWLARLETLHPSAIELGLERVRRVKDAMGLAPDFPLIVVGGTNGKGSTCAYLEAVFGAAGYKTGLYTSPHLLR
YNERVRIAGKEVDDADLVAAFEAVDRARGDVALTYFEFGTLAAMAQFVAAKVDVAILEVGLGGRLDAVNVFDADAAIVTS
VDLDHMDYLGDTREQIGFEKAGIYRAGRPAVCADPEPPASLLAHAGKVGADLRRLGRDFSAARAGERWTYHSRALSWPDL
PLPAMAGPCQLRNAAGALAVLEALQARLPTSSEAIRRGVAEARVAGRFQRIGRRPEVIVDVAHNPEAARALASTLREQPI
SGRTLAVVGMLADKDAGAVVAALADGVDAWWTCTPDSPRARDASSLAAVLHAHGVRSVNVQPGVKAALAAARSAAGEDDR
ILVFGSFYTVAAVLDHAATQQ

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=169, Percent_Identity=37.8698224852071, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI66932990, Length=169, Percent_Identity=37.8698224852071, Blast_Score=122, Evalue=4e-28,
Organism=Escherichia coli, GI1788654, Length=417, Percent_Identity=44.6043165467626, Blast_Score=331, Evalue=6e-92,
Organism=Caenorhabditis elegans, GI17553148, Length=195, Percent_Identity=33.8461538461538, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17553150, Length=195, Percent_Identity=33.8461538461538, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI71984923, Length=195, Percent_Identity=33.8461538461538, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6323760, Length=428, Percent_Identity=27.1028037383178, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6324815, Length=349, Percent_Identity=27.5071633237822, Blast_Score=113, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24641571, Length=162, Percent_Identity=37.037037037037, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24581568, Length=180, Percent_Identity=34.4444444444444, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17; =6.3.2.12 [H]

Molecular weight: Translated: 44440; Mature: 44309

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPSASLAAWLARLETLHPSAIELGLERVRRVKDAMGLAPDFPLIVVGGTNGKGSTCAYL
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHH
EAVFGAAGYKTGLYTSPHLLRYNERVRIAGKEVDDADLVAAFEAVDRARGDVALTYFEFG
HHHHHCCCCCCCCCCCCHHEEECCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEHHH
TLAAMAQFVAAKVDVAILEVGLGGRLDAVNVFDADAAIVTSVDLDHMDYLGDTREQIGFE
HHHHHHHHHHHHHCEEEEEECCCCCCCEEEEECCCCEEEEECCHHHHHHHCCHHHHHCCH
KAGIYRAGRPAVCADPEPPASLLAHAGKVGADLRRLGRDFSAARAGERWTYHSRALSWPD
HHCCEECCCCCCCCCCCCHHHHHHHCCCCCHHHHHHCCHHHHHHCCCCCEECCCCCCCCC
LPLPAMAGPCQLRNAAGALAVLEALQARLPTSSEAIRRGVAEARVAGRFQRIGRRPEVIV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEE
DVAHNPEAARALASTLREQPISGRTLAVVGMLADKDAGAVVAALADGVDAWWTCTPDSPR
EECCCCHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCHHHHHHHCCCCCEEEECCCCCC
ARDASSLAAVLHAHGVRSVNVQPGVKAALAAARSAAGEDDRILVFGSFYTVAAVLDHAAT
CCCHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHC
QQ
CC
>Mature Secondary Structure 
PPSASLAAWLARLETLHPSAIELGLERVRRVKDAMGLAPDFPLIVVGGTNGKGSTCAYL
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHH
EAVFGAAGYKTGLYTSPHLLRYNERVRIAGKEVDDADLVAAFEAVDRARGDVALTYFEFG
HHHHHCCCCCCCCCCCCHHEEECCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEHHH
TLAAMAQFVAAKVDVAILEVGLGGRLDAVNVFDADAAIVTSVDLDHMDYLGDTREQIGFE
HHHHHHHHHHHHHCEEEEEECCCCCCCEEEEECCCCEEEEECCHHHHHHHCCHHHHHCCH
KAGIYRAGRPAVCADPEPPASLLAHAGKVGADLRRLGRDFSAARAGERWTYHSRALSWPD
HHCCEECCCCCCCCCCCCHHHHHHHCCCCCHHHHHHCCHHHHHHCCCCCEECCCCCCCCC
LPLPAMAGPCQLRNAAGALAVLEALQARLPTSSEAIRRGVAEARVAGRFQRIGRRPEVIV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEE
DVAHNPEAARALASTLREQPISGRTLAVVGMLADKDAGAVVAALADGVDAWWTCTPDSPR
EECCCCHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCHHHHHHHCCCCCEEEECCCCCC
ARDASSLAAVLHAHGVRSVNVQPGVKAALAAARSAAGEDDRILVFGSFYTVAAVLDHAAT
CCCHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHC
QQ
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3040739; 3040734; 9205837; 9278503; 1989505 [H]