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The map label for this gene is pilR [H]

Identifier: 74317825

GI number: 74317825

Start: 1909872

End: 1911194

Strand: Direct

Name: pilR [H]

Synonym: Tbd_1807

Alternate gene names: 74317825

Gene position: 1909872-1911194 (Clockwise)

Preceding gene: 74317824

Following gene: 74317826

Centisome position: 65.64

GC content: 66.44

Gene sequence:

>1323_bases
ATGAAACCCTCGAACCGCCCGCGCATCCTGCTCGTCGACGACGAGCCCGACCTGCTCGACCTGATGGAGCTCACGCTTCT
CAAGATGGGCCTCGAAACCGACCGCGCGCTGACCGTCACCGACGCCTGTTCCCGGCTCGCGAATACGCCTTATGACTTGT
GCCTGACCGACATGCGCCTCGGCGACGGCGAAGGTCTGGAAGTCATCGCCGCGGCCAATGCCCTGCCGGTCCCGGTCCCC
GCGGCCGTCATCACGGCTTACGGCAACGCCGGCAACGCCGTCGCGGCGCTCAAGGCCGGCGCCTTCGACTACCTCGCCAA
GCCGGTGGCGCTCGATCAATTGCGCACGCTCGTGAAGTCCGCGCTGCAGGTCCCGGCCTCGTCGGGTCAGGCGGCGACAG
CCCGCGGGCTCATCGGAAACTCGAGCGCGATGCAGGACATCCGTGCCCGCATCGCCAAGCTCGCGCGCACGCAGGCGCCG
GTTCACATCAGCGGCGAGTCAGGCAGCGGCAAGGAGCTCGCCGCACGTCTGATCCACGAAACGAGCAATCGGGCCGACAA
GCCGCTGGTCGCCGTCAACTGTGGCGCGATTCCCGAGGCGCTCATGGAGAGCGAATTCTTCGGCTATCGCAAGGGCGCCT
TCACGGGTGCGGAAGCCGATCGCACCGGCTTCTTCCAAGCGGCCAACGGCGGCACGCTCTTCCTCGACGAGGTCGGCGAC
CTGCCGCTGTCGATGCAGGTCAAGCTGCTGCGCGTGCTGCAGGAAAAGAAGGTGCGCAAGGTCGGCGCGACCACCGAGGA
AGACGTCGACGTGCGCATCATCAGCGCGACGCACCAGAATCTCGCGGCACTCGTCGAAGCCGGCCGATTCCGCCAGGATC
TCTATTACCGGCTCAACGTCATCGACCTGCTCATGCCGAGCCTGCGCGACCGCGCCGAGGACATCCCGGAAATGGCGCGC
TTTCTGCTCGAAAAGCTCGGCGGACGCGCGGTCGAACTCGAGCGCGACGCCGAAAAGGCACTCTGCACCTACGCTTTCCC
CGGCAACGTGCGTGAACTCGAAAACATCCTCGAACGTGCGCTCGCCCTGTGCGAGAACGCGCGCATCCGGGCCGCGGACC
TCAACCTCGTCCCCCCGCCGCCTTCGACCGCGGCGATGCCGGGAAGCAAGTATCCACTGCAGGACTACCTGGATCAGACC
GAACGCGCGGCCATCATCGAAGCGCTCGAGCAGACTCGATACAACAAGACCGCGGCGGCGCGGCTACTCGGCGTGACCTT
TCGCAGCCTGCGCTATCGGCTGGAACGCCTGGGCATCGAATAG

Upstream 100 bases:

>100_bases
TCTTCCTCGCCCGCGAACTGGCCGAAGCGAACCGCGCGGCGCTCGTCTACCTGCCCGGAGCCGGCGGCGCGAATTTCCGC
CTGAGCCTGCGTCAGGACAC

Downstream 100 bases:

>100_bases
GGTTTCCGGCGGTCCAAAAGAAAAAATCCGCAGGCCCAGCGGGCTGCGGATTTCCGGAGACGATGGTGCCGCTTGTCGGA
TTCGAACTGACGACCTACCG

Product: two component Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MKPSNRPRILLVDDEPDLLDLMELTLLKMGLETDRALTVTDACSRLANTPYDLCLTDMRLGDGEGLEVIAAANALPVPVP
AAVITAYGNAGNAVAALKAGAFDYLAKPVALDQLRTLVKSALQVPASSGQAATARGLIGNSSAMQDIRARIAKLARTQAP
VHISGESGSGKELAARLIHETSNRADKPLVAVNCGAIPEALMESEFFGYRKGAFTGAEADRTGFFQAANGGTLFLDEVGD
LPLSMQVKLLRVLQEKKVRKVGATTEEDVDVRIISATHQNLAALVEAGRFRQDLYYRLNVIDLLMPSLRDRAEDIPEMAR
FLLEKLGGRAVELERDAEKALCTYAFPGNVRELENILERALALCENARIRAADLNLVPPPPSTAAMPGSKYPLQDYLDQT
ERAAIIEALEQTRYNKTAAARLLGVTFRSLRYRLERLGIE

Sequences:

>Translated_440_residues
MKPSNRPRILLVDDEPDLLDLMELTLLKMGLETDRALTVTDACSRLANTPYDLCLTDMRLGDGEGLEVIAAANALPVPVP
AAVITAYGNAGNAVAALKAGAFDYLAKPVALDQLRTLVKSALQVPASSGQAATARGLIGNSSAMQDIRARIAKLARTQAP
VHISGESGSGKELAARLIHETSNRADKPLVAVNCGAIPEALMESEFFGYRKGAFTGAEADRTGFFQAANGGTLFLDEVGD
LPLSMQVKLLRVLQEKKVRKVGATTEEDVDVRIISATHQNLAALVEAGRFRQDLYYRLNVIDLLMPSLRDRAEDIPEMAR
FLLEKLGGRAVELERDAEKALCTYAFPGNVRELENILERALALCENARIRAADLNLVPPPPSTAAMPGSKYPLQDYLDQT
ERAAIIEALEQTRYNKTAAARLLGVTFRSLRYRLERLGIE
>Mature_440_residues
MKPSNRPRILLVDDEPDLLDLMELTLLKMGLETDRALTVTDACSRLANTPYDLCLTDMRLGDGEGLEVIAAANALPVPVP
AAVITAYGNAGNAVAALKAGAFDYLAKPVALDQLRTLVKSALQVPASSGQAATARGLIGNSSAMQDIRARIAKLARTQAP
VHISGESGSGKELAARLIHETSNRADKPLVAVNCGAIPEALMESEFFGYRKGAFTGAEADRTGFFQAANGGTLFLDEVGD
LPLSMQVKLLRVLQEKKVRKVGATTEEDVDVRIISATHQNLAALVEAGRFRQDLYYRLNVIDLLMPSLRDRAEDIPEMAR
FLLEKLGGRAVELERDAEKALCTYAFPGNVRELENILERALALCENARIRAADLNLVPPPPSTAAMPGSKYPLQDYLDQT
ERAAIIEALEQTRYNKTAAARLLGVTFRSLRYRLERLGIE

Specific function: Member of the two-component regulatory system pilS/pilR that regulates the expression of type 4 fimbriae. When activated by pilS it acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=455, Percent_Identity=40.8791208791209, Blast_Score=332, Evalue=3e-92,
Organism=Escherichia coli, GI1790437, Length=442, Percent_Identity=43.4389140271493, Blast_Score=325, Evalue=4e-90,
Organism=Escherichia coli, GI1788905, Length=439, Percent_Identity=40.5466970387244, Blast_Score=291, Evalue=4e-80,
Organism=Escherichia coli, GI1790299, Length=464, Percent_Identity=37.9310344827586, Blast_Score=288, Evalue=6e-79,
Organism=Escherichia coli, GI87082117, Length=318, Percent_Identity=43.3962264150943, Blast_Score=248, Evalue=4e-67,
Organism=Escherichia coli, GI1789233, Length=319, Percent_Identity=40.4388714733542, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI87082152, Length=328, Percent_Identity=42.0731707317073, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1789087, Length=317, Percent_Identity=39.7476340694006, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1786524, Length=326, Percent_Identity=42.0245398773006, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1787583, Length=341, Percent_Identity=37.2434017595308, Blast_Score=195, Evalue=4e-51,
Organism=Escherichia coli, GI87081872, Length=321, Percent_Identity=35.8255451713396, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI87081858, Length=290, Percent_Identity=32.4137931034483, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789828, Length=233, Percent_Identity=35.1931330472103, Blast_Score=134, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 47768; Mature: 47768

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSNRPRILLVDDEPDLLDLMELTLLKMGLETDRALTVTDACSRLANTPYDLCLTDMRL
CCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEHHHHHHHHCCCCHHHHHHHCCC
GDGEGLEVIAAANALPVPVPAAVITAYGNAGNAVAALKAGAFDYLAKPVALDQLRTLVKS
CCCCCEEEEEECCCCCCCCCHHHHEEECCCCCCHHHHHHCCHHHHHCHHHHHHHHHHHHH
ALQVPASSGQAATARGLIGNSSAMQDIRARIAKLARTQAPVHISGESGSGKELAARLIHE
HHHCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHH
TSNRADKPLVAVNCGAIPEALMESEFFGYRKGAFTGAEADRTGFFQAANGGTLFLDEVGD
HCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCEEEEECCCC
LPLSMQVKLLRVLQEKKVRKVGATTEEDVDVRIISATHQNLAALVEAGRFRQDLYYRLNV
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
IDLLMPSLRDRAEDIPEMARFLLEKLGGRAVELERDAEKALCTYAFPGNVRELENILERA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHCEEEEECCCCHHHHHHHHHHH
LALCENARIRAADLNLVPPPPSTAAMPGSKYPLQDYLDQTERAAIIEALEQTRYNKTAAA
HHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHH
RLLGVTFRSLRYRLERLGIE
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKPSNRPRILLVDDEPDLLDLMELTLLKMGLETDRALTVTDACSRLANTPYDLCLTDMRL
CCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEHHHHHHHHCCCCHHHHHHHCCC
GDGEGLEVIAAANALPVPVPAAVITAYGNAGNAVAALKAGAFDYLAKPVALDQLRTLVKS
CCCCCEEEEEECCCCCCCCCHHHHEEECCCCCCHHHHHHCCHHHHHCHHHHHHHHHHHHH
ALQVPASSGQAATARGLIGNSSAMQDIRARIAKLARTQAPVHISGESGSGKELAARLIHE
HHHCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHH
TSNRADKPLVAVNCGAIPEALMESEFFGYRKGAFTGAEADRTGFFQAANGGTLFLDEVGD
HCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCEEEEECCCC
LPLSMQVKLLRVLQEKKVRKVGATTEEDVDVRIISATHQNLAALVEAGRFRQDLYYRLNV
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
IDLLMPSLRDRAEDIPEMARFLLEKLGGRAVELERDAEKALCTYAFPGNVRELENILERA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHCEEEEECCCCHHHHHHHHHHH
LALCENARIRAADLNLVPPPPSTAAMPGSKYPLQDYLDQTERAAIIEALEQTRYNKTAAA
HHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHH
RLLGVTFRSLRYRLERLGIE
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1317379; 8097014; 7911557; 10984043 [H]