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The map label for this gene is rfbB [H]

Identifier: 74317798

GI number: 74317798

Start: 1879276

End: 1880343

Strand: Reverse

Name: rfbB [H]

Synonym: Tbd_1780

Alternate gene names: 74317798

Gene position: 1880343-1879276 (Counterclockwise)

Preceding gene: 74317799

Following gene: 74317797

Centisome position: 64.62

GC content: 63.39

Gene sequence:

>1068_bases
GTGATATTGGTTACGGGGGGCGCCGGTTTCATCGGCGCAAATTTTGTTCTCGACTGGCTTCGCGCAAACGACGAGCCGGT
GCTCGATCTGGACAAGCTCACCTATGCGGGCAATCCGGAAAACCTCGCGGCGCTGCAGGGCGACCCGCGCCACATCTTCG
TGCAGGGCGACATCGGCGACCGTGGACTGGTCGGCAAATTGCTCAGCGAACACCGCCCGCGCGCGATCGTCAACTTCGCC
GCCGAGTCGCACGTCGACCGTTCGATCCACGGCCCGGAAGATTTCATCCAGACCAACGTCGTCGGCACCTTCCATCTGCT
CGACGAGGCGCGGGCCTACTGGGTGAGTCTTCCCGAGGAGGAGAAGGCGGCGTTCCGCTTTCTGCACGTGTCGACCGACG
AAGTCTACGGCTCGCTCGGCCCGACAGACGCGCCCTTCACCGAGCAGACGCCGTTCGCGCCCAACAGCCCTTACTCGGCG
AGCAAGGCGTCGTCCGATCATCTCGTGCGCGCCTATCACCACACCTACGGGCTGCCGGTGCTGACGACCAACTGCTCCAA
CAACTACGGTCCCTACCAATTCCCCGAAAAACTCATTCCGCTGATCGTGCTCAATGCCGTGCAGGGCAAGCCCCTGCCGA
TCTACGGCGACGGCATGAACGTGCGCGACTGGCTGTATGTCGGGGACCATTGCACGGCGATCCGCGCGGCGCTCGAAAAG
GGCCGACCGGGCGAAACGTACAATATCGGCGGCTGGAACGAGATGCCGAACATCGAGATCGTCAAGACGGTGTGCGCGCT
GCTCGACGAGATGCGGCCCGATCCGGCGGGCCCGTACGCGCGCTTGATCACCTATGTGACCGATCGCCCGGGACACGACC
GGCGCTACGCGATCGACGCGAGCAAGATCGACCGCGAACTCGGCTGGCGGCCGGCCGAGACCTTCGAAACCGGGATCCGC
AAGACGGTGCGCTGGTACCTCGACAACATGGCCTGGGTCGAACACGTCGCGAGCGGCGCGTATCGCAATTGGGTCGAAAC
GAATTATTCAGAAAGAGTTCAGGCATGA

Upstream 100 bases:

>100_bases
GCGTTCACTTGGGAGAAAATCCTGGGTGATTACGAGAAGCTGTTGCTGGACTGGGCGTAGCGCTCACGATCCAGCCGTTA
TTTTTTCAAGGAAGATCATC

Downstream 100 bases:

>100_bases
AGCGCAAGGGCATCATCCTCGCCGGCGGTTCCGGTACCCGCCTCTATCCGGCGACCCTGGCAGTCTCGAAGCAGCTGCTG
CCGATTTACGACAAGCCGAT

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MILVTGGAGFIGANFVLDWLRANDEPVLDLDKLTYAGNPENLAALQGDPRHIFVQGDIGDRGLVGKLLSEHRPRAIVNFA
AESHVDRSIHGPEDFIQTNVVGTFHLLDEARAYWVSLPEEEKAAFRFLHVSTDEVYGSLGPTDAPFTEQTPFAPNSPYSA
SKASSDHLVRAYHHTYGLPVLTTNCSNNYGPYQFPEKLIPLIVLNAVQGKPLPIYGDGMNVRDWLYVGDHCTAIRAALEK
GRPGETYNIGGWNEMPNIEIVKTVCALLDEMRPDPAGPYARLITYVTDRPGHDRRYAIDASKIDRELGWRPAETFETGIR
KTVRWYLDNMAWVEHVASGAYRNWVETNYSERVQA

Sequences:

>Translated_355_residues
MILVTGGAGFIGANFVLDWLRANDEPVLDLDKLTYAGNPENLAALQGDPRHIFVQGDIGDRGLVGKLLSEHRPRAIVNFA
AESHVDRSIHGPEDFIQTNVVGTFHLLDEARAYWVSLPEEEKAAFRFLHVSTDEVYGSLGPTDAPFTEQTPFAPNSPYSA
SKASSDHLVRAYHHTYGLPVLTTNCSNNYGPYQFPEKLIPLIVLNAVQGKPLPIYGDGMNVRDWLYVGDHCTAIRAALEK
GRPGETYNIGGWNEMPNIEIVKTVCALLDEMRPDPAGPYARLITYVTDRPGHDRRYAIDASKIDRELGWRPAETFETGIR
KTVRWYLDNMAWVEHVASGAYRNWVETNYSERVQA
>Mature_355_residues
MILVTGGAGFIGANFVLDWLRANDEPVLDLDKLTYAGNPENLAALQGDPRHIFVQGDIGDRGLVGKLLSEHRPRAIVNFA
AESHVDRSIHGPEDFIQTNVVGTFHLLDEARAYWVSLPEEEKAAFRFLHVSTDEVYGSLGPTDAPFTEQTPFAPNSPYSA
SKASSDHLVRAYHHTYGLPVLTTNCSNNYGPYQFPEKLIPLIVLNAVQGKPLPIYGDGMNVRDWLYVGDHCTAIRAALEK
GRPGETYNIGGWNEMPNIEIVKTVCALLDEMRPDPAGPYARLITYVTDRPGHDRRYAIDASKIDRELGWRPAETFETGIR
KTVRWYLDNMAWVEHVASGAYRNWVETNYSERVQA

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=332, Percent_Identity=37.0481927710843, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI42516563, Length=338, Percent_Identity=24.5562130177515, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI56237023, Length=356, Percent_Identity=26.9662921348315, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI56118217, Length=356, Percent_Identity=26.9662921348315, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI189083684, Length=356, Percent_Identity=26.9662921348315, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI4504031, Length=343, Percent_Identity=25.0728862973761, Blast_Score=75, Evalue=8e-14,
Organism=Escherichia coli, GI1788353, Length=359, Percent_Identity=58.2172701949861, Blast_Score=433, Evalue=1e-123,
Organism=Escherichia coli, GI48994969, Length=344, Percent_Identity=59.5930232558139, Blast_Score=423, Evalue=1e-120,
Organism=Escherichia coli, GI1786974, Length=349, Percent_Identity=28.3667621776504, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788366, Length=351, Percent_Identity=24.5014245014245, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17568069, Length=334, Percent_Identity=38.0239520958084, Blast_Score=208, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI115532424, Length=347, Percent_Identity=31.9884726224784, Blast_Score=171, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI17539532, Length=330, Percent_Identity=23.9393939393939, Blast_Score=78, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI133901786, Length=292, Percent_Identity=25.3424657534247, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17507723, Length=342, Percent_Identity=23.9766081871345, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17539422, Length=292, Percent_Identity=25.3424657534247, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI133901788, Length=292, Percent_Identity=25.3424657534247, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17539424, Length=292, Percent_Identity=25.3424657534247, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI133901790, Length=292, Percent_Identity=25.3424657534247, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6319493, Length=363, Percent_Identity=24.2424242424242, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19923002, Length=360, Percent_Identity=29.4444444444444, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24158427, Length=335, Percent_Identity=26.5671641791045, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21356223, Length=338, Percent_Identity=25.4437869822485, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39698; Mature: 39698

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILVTGGAGFIGANFVLDWLRANDEPVLDLDKLTYAGNPENLAALQGDPRHIFVQGDIGD
CEEEECCCCHHHHHHHHHHHHCCCCCCEEHHHEEECCCCCCEEEECCCCCEEEEECCCCC
RGLVGKLLSEHRPRAIVNFAAESHVDRSIHGPEDFIQTNVVGTFHLLDEARAYWVSLPEE
CHHHHHHHHCCCCHHEEEHHHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHEEEECCCH
EKAAFRFLHVSTDEVYGSLGPTDAPFTEQTPFAPNSPYSASKASSDHLVRAYHHTYGLPV
HCCEEEEEEECHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCE
LTTNCSNNYGPYQFPEKLIPLIVLNAVQGKPLPIYGDGMNVRDWLYVGDHCTAIRAALEK
EEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEECCHHHHHHHHHHC
GRPGETYNIGGWNEMPNIEIVKTVCALLDEMRPDPAGPYARLITYVTDRPGHDRRYAIDA
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCEEEECH
SKIDRELGWRPAETFETGIRKTVRWYLDNMAWVEHVASGAYRNWVETNYSERVQA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCC
>Mature Secondary Structure
MILVTGGAGFIGANFVLDWLRANDEPVLDLDKLTYAGNPENLAALQGDPRHIFVQGDIGD
CEEEECCCCHHHHHHHHHHHHCCCCCCEEHHHEEECCCCCCEEEECCCCCEEEEECCCCC
RGLVGKLLSEHRPRAIVNFAAESHVDRSIHGPEDFIQTNVVGTFHLLDEARAYWVSLPEE
CHHHHHHHHCCCCHHEEEHHHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHEEEECCCH
EKAAFRFLHVSTDEVYGSLGPTDAPFTEQTPFAPNSPYSASKASSDHLVRAYHHTYGLPV
HCCEEEEEEECHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCE
LTTNCSNNYGPYQFPEKLIPLIVLNAVQGKPLPIYGDGMNVRDWLYVGDHCTAIRAALEK
EEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEECCHHHHHHHHHHC
GRPGETYNIGGWNEMPNIEIVKTVCALLDEMRPDPAGPYARLITYVTDRPGHDRRYAIDA
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCEEEECH
SKIDRELGWRPAETFETGIRKTVRWYLDNMAWVEHVASGAYRNWVETNYSERVQA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]