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The map label for this gene is fcl [H]

Identifier: 74317793

GI number: 74317793

Start: 1874762

End: 1875745

Strand: Reverse

Name: fcl [H]

Synonym: Tbd_1775

Alternate gene names: 74317793

Gene position: 1875745-1874762 (Counterclockwise)

Preceding gene: 74317794

Following gene: 74317792

Centisome position: 64.46

GC content: 64.33

Gene sequence:

>984_bases
ATGGACAAGAACGCGCGCATTTATGTGGCGGGGCACCGCGGCCTCGTCGGCTCCGCGCTGATGCGCGATCTCGAACGCAA
GGGCTACGGCAACTTCGTCACCCGCACCCACGCGGAACTCGACCTGACCAAGCAGGCGGCGGTCGAAGCTTTTTTTGCCA
GCGAGAGGCCCGACTACGTGTTTCTCGCTGCGGCGAAGGTCGGCGGCATCCACGCCAACGACACTTATCCCGCCGACTTC
ATTCGCGACAACCTCGCGATCCAGACCAACGTCATCCATGCGGCTTGGAAGAGCGACGTCAAACGCCTGCTGTTTCTCGG
CTCGAGCTGCATTTATCCGAAGTTCGCGCCCCAGCCGATGAAGGAGGCGCACCTGCTCGACGGGGAACTGGAGCCGACGA
ACCGGCCCTATGCGCTGGCGAAGATCGCGGGCATCGAGATGTGCTGGGCCTACAACCGCCAGTACAAGACCCAGTACCTC
GCCGTCATGCCGACCAATTTGTACGGGCCCGGCGACAACTACCATCCCGAGAACTCGCACGTGATCCCGGCGCTGATCCG
TCGCTTCCACGAGGCCAAGCAGGCGGGCGCGCCGAGCGTGAGCGTTTGGGGCAGCGGCACGCCCCGACGCGAATTTCTCT
ACAGCGAGGACATGGCCGACGCCTGCACCCATTTGATGGCGCTGCCGGACGCCGAATTCCTGCCCTTGCTCGGACAGGAC
CGCAACGACGGCCTGGCGCCGCTCGTGAACATCGGCGTCGGCCACGACCTCACGATTCGCGAACTCGCCGAGACGGTGAA
ATCCGTCGTCGGCTACCCCGGCGACATCGTGTTCGACGCGAGCAAGCCGGACGGCACGCCCCGAAAGCTCTTGGACGTGA
GCCGGCTGAACGCGATGGGTTGGCAGGCGCGCACCGAAATGGGGACGGGACTGCGGCGTGCATACGAGGACTTCCTGAGC
GCGAAATCTAGCGAAAGCACCTGA

Upstream 100 bases:

>100_bases
ACTCGTCACCGAAATGGTGCGCGAAGACCTCAAGGCGGCCGAGCGCGACGAGCTGGTGAAAAAGCACGGCTACAAAGCCA
TGGATTACCACGAATAAACG

Downstream 100 bases:

>100_bases
TTCGCGCGCCCGCACAGCCGGCCATGGACACGTGCCGCAGGCTGACGGTTTGTGCGCGGCGCCCAGCCTACTGACATCCG
CATGGCGGCAACAGGAGAAG

Product: GDP-L-fucose synthetase

Products: NA

Alternate protein names: GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MDKNARIYVAGHRGLVGSALMRDLERKGYGNFVTRTHAELDLTKQAAVEAFFASERPDYVFLAAAKVGGIHANDTYPADF
IRDNLAIQTNVIHAAWKSDVKRLLFLGSSCIYPKFAPQPMKEAHLLDGELEPTNRPYALAKIAGIEMCWAYNRQYKTQYL
AVMPTNLYGPGDNYHPENSHVIPALIRRFHEAKQAGAPSVSVWGSGTPRREFLYSEDMADACTHLMALPDAEFLPLLGQD
RNDGLAPLVNIGVGHDLTIRELAETVKSVVGYPGDIVFDASKPDGTPRKLLDVSRLNAMGWQARTEMGTGLRRAYEDFLS
AKSSEST

Sequences:

>Translated_327_residues
MDKNARIYVAGHRGLVGSALMRDLERKGYGNFVTRTHAELDLTKQAAVEAFFASERPDYVFLAAAKVGGIHANDTYPADF
IRDNLAIQTNVIHAAWKSDVKRLLFLGSSCIYPKFAPQPMKEAHLLDGELEPTNRPYALAKIAGIEMCWAYNRQYKTQYL
AVMPTNLYGPGDNYHPENSHVIPALIRRFHEAKQAGAPSVSVWGSGTPRREFLYSEDMADACTHLMALPDAEFLPLLGQD
RNDGLAPLVNIGVGHDLTIRELAETVKSVVGYPGDIVFDASKPDGTPRKLLDVSRLNAMGWQARTEMGTGLRRAYEDFLS
AKSSEST
>Mature_327_residues
MDKNARIYVAGHRGLVGSALMRDLERKGYGNFVTRTHAELDLTKQAAVEAFFASERPDYVFLAAAKVGGIHANDTYPADF
IRDNLAIQTNVIHAAWKSDVKRLLFLGSSCIYPKFAPQPMKEAHLLDGELEPTNRPYALAKIAGIEMCWAYNRQYKTQYL
AVMPTNLYGPGDNYHPENSHVIPALIRRFHEAKQAGAPSVSVWGSGTPRREFLYSEDMADACTHLMALPDAEFLPLLGQD
RNDGLAPLVNIGVGHDLTIRELAETVKSVVGYPGDIVFDASKPDGTPRKLLDVSRLNAMGWQARTEMGTGLRRAYEDFLS
AKSSEST

Specific function: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=295, Percent_Identity=33.5593220338983, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI42516563, Length=330, Percent_Identity=24.8484848484848, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1788365, Length=314, Percent_Identity=60.1910828025478, Blast_Score=391, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17554428, Length=286, Percent_Identity=30.0699300699301, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI19922778, Length=304, Percent_Identity=31.9078947368421, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI21356223, Length=332, Percent_Identity=25.9036144578313, Blast_Score=71, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.271 [H]

Molecular weight: Translated: 36173; Mature: 36173

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKNARIYVAGHRGLVGSALMRDLERKGYGNFVTRTHAELDLTKQAAVEAFFASERPDYV
CCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHHHCCCCCEE
FLAAAKVGGIHANDTYPADFIRDNLAIQTNVIHAAWKSDVKRLLFLGSSCIYPKFAPQPM
EEEEEEECCEECCCCCCHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
KEAHLLDGELEPTNRPYALAKIAGIEMCWAYNRQYKTQYLAVMPTNLYGPGDNYHPENSH
HHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCC
VIPALIRRFHEAKQAGAPSVSVWGSGTPRREFLYSEDMADACTHLMALPDAEFLPLLGQD
CHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCC
RNDGLAPLVNIGVGHDLTIRELAETVKSVVGYPGDIVFDASKPDGTPRKLLDVSRLNAMG
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHCC
WQARTEMGTGLRRAYEDFLSAKSSEST
CHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDKNARIYVAGHRGLVGSALMRDLERKGYGNFVTRTHAELDLTKQAAVEAFFASERPDYV
CCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHHHCCCCCEE
FLAAAKVGGIHANDTYPADFIRDNLAIQTNVIHAAWKSDVKRLLFLGSSCIYPKFAPQPM
EEEEEEECCEECCCCCCHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
KEAHLLDGELEPTNRPYALAKIAGIEMCWAYNRQYKTQYLAVMPTNLYGPGDNYHPENSH
HHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCC
VIPALIRRFHEAKQAGAPSVSVWGSGTPRREFLYSEDMADACTHLMALPDAEFLPLLGQD
CHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCC
RNDGLAPLVNIGVGHDLTIRELAETVKSVVGYPGDIVFDASKPDGTPRKLLDVSRLNAMG
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHCC
WQARTEMGTGLRRAYEDFLSAKSSEST
CHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503; 9473059; 9862812 [H]