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The map label for this gene is relA [H]

Identifier: 74317776

GI number: 74317776

Start: 1848989

End: 1851157

Strand: Reverse

Name: relA [H]

Synonym: Tbd_1758

Alternate gene names: 74317776

Gene position: 1851157-1848989 (Counterclockwise)

Preceding gene: 74317777

Following gene: 74317773

Centisome position: 63.62

GC content: 68.14

Gene sequence:

>2169_bases
ATGAACGAAATTGTCCGCCCGCGCTGCTACGAAGCGACCGACCGCGAGTCGGCGCGGCAAGCCCTCGCACGCGTGCTGCC
CGTTGCCGAAAACGATCTGCCCTGGCGCGCGCTCGACTACGCCTACGAGCGGCTCGGCGGCAGCGAGGCGTTCGCGCACA
GCGTGGGCAGTGCACTGATCGCCGCCGAACTCAAGGTCGGTGCCGACGCGGTGGCGGCGGCGCTGCTGCACGCCTGTCTT
GGTGACGAGGCGGAGATGGATGAGCGCTTCAACAGCGCTGCCCAGCTCGCGCGCGGGGTCGCGGCGATGGCCCGCATCGA
GTCGCTGGCCGCCACGACGACCGACAAACGCATCGATCCCCACGCCCAGCTCGAAGCCTTGCGTCAGATGGTGCTGGCGA
TGGTCGAGGACATTCGCGTGGTGTTGCTGAAGCTCGCCGAGCGTACCCACGCGCTGCGTTGCGCCGCGTCGCCCGCGCCG
GAGGGCGACGACCCCGACGAACGCGCGGCGCTGCGCGAGGCGCTCGGCCAACAGGCCCGCGAGCTCTTCGCCCCGCTGGC
CAACCGTCTCGGCGTGTGGCAGATCAAGTGGGAGATGGAGGACTGGGCCTTCCGCTACCTCGAACCGGAAACCTACCGCA
CGATCGCCGCGCAACTCGACGAAAAGCGCGCCGATCGCGAGGCCTACATCCTCGGGATCATCGAACGCCTGAAGGCCGAA
CTGGCGCTGCACGGCATCGAGGCCGAGGTGACGGGCCGGCCCAAGCACATCGCGAGTATCGTCAACAAGATGCGGCGCAA
GCATCTGAGCTTCGAGCAGTTGTACGACATCCGTGCCGTGCGCGTCCTGGTGCGCCAGGAAATCGACTGCTACACCGTGC
TCGGCCTCGTGCACAACCTGTGGCAGCCGATTCCCGGTGAGTTCGACGATTACATCTCGCAGCCCAAGAGCAACGACTAC
CGCTCGCTTCACACCGCGGTCATCGGGCCCGAGGATCGCGCGCTCGAGGTGCAGATACGCACCTTCGACATGCATCGCCA
CGCCGAACTCGGTGTCGCGGCGCACTGGCGCTACAAGGAAAGCGGCAAGCAGGACGCGCAGTACGAGGAGAAGATCGCGT
GGCTGCGGCGCATCCTCGAATGGAAGGACGACGTCGCCGACAGCAGCGAATTCCGCGAGCAGTTCAAGACCGAGCTCTTC
CACGACCAGGTCTATGTGCTGACGCCGCAGGGGCGGGTCGTCGCGCTCGACCGCGGCGCGACCCCGGTGGATTTCGCCTA
CGCCGTGCATACCGATCTCGGCCATCGCTGCCGCGGCGCGAAGGTCAACGGCGCGATGGTCCCGCTCAACACGGTTCTCG
ACAACAGCCAGCGCGTCGAGATCGTGACCGCCAAGGAAGGCACGCCGAGCCGCGACTGGCTCAATCCGGCACTTGGCTAT
CTCGCGACGCATCGCGCGCGGTCGAAGGTGCGGGCGTGGTTCCGTCAGCGCGACGTCGGCGAGCAGGTGAACCTCGGCCG
CAGCCTGCTCGACAAGGAACTCAAGCGCCTCGGCGTCGGCGACGTGAACCACGAAAAGCTCGCCCAGCGCCTCAAGTTTT
CCAGTGTCGACGAGTTCTTCGCCGCGCTCGGGCGCGGCGACGTCGGCCAGCGCGATCTGGTCGGCGCGCTACAGGAGCCG
GGCAAGGCGCCCCCAGCGCTTGCGCCGACGGCCAAGCCGCGGTCCCGTCCGGGCTCGGGCAGCCCGGTCGTCATTCCGGG
ACTCGGCGATGTGCCGCTGACGATGGCGCGCTGCTGCAAGCCGCGGCCGCCCGAGGCGATCGTCGCCTACACCACGGTCG
GGCGCGGCGTCACCGTGCACCGGGCCGACTGCGCGTCGCTCAGGCGCGCCAACCAGGCCCGGCAGATGCCCGCCGAATGG
GCGCTCGATCTCGGCACCGGTTTCGAGGTCGACATCCGGCTCAAGGCCTTCGATCGTCAGGGCTTGCTGCGCGACGTGTC
GGACGTGATTGCGAAGGACAAGCTCGATGTGCTGCGCGTGAACACCGAGACCCGCGGCGAGTACGCGCAGATGCAGCTCA
CGGTCCGCATCAAGGAGCTGCAGCAGCTGTCCCGCCTGCTGGCCCGCCTGCAGCACGTGCAGAACGTCGTCGAGGTGAGG
CGCAGCTAG

Upstream 100 bases:

>100_bases
GCGATCGGGCGGGGCTCGTGCGCCTGACGTTTGGACAAGCCGGAGTGACGGCCTCAGAATATCGACCCGCCCACCGCCGT
TACTGGCACGCCCCCCCGTC

Downstream 100 bases:

>100_bases
GGTTTTGCAGCCAGGCGCCCGCGGCTCAGGGCGCGGCGTCGAGGATCGCGCGGGCGAGGTCGCGCAGGCCGGCAGGCGAG
AGATCGGCGGCGTGAAACGT

Product: GTP diphosphokinase, RelA/SpoT protein

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase; ppGpp synthase I [H]

Number of amino acids: Translated: 722; Mature: 722

Protein sequence:

>722_residues
MNEIVRPRCYEATDRESARQALARVLPVAENDLPWRALDYAYERLGGSEAFAHSVGSALIAAELKVGADAVAAALLHACL
GDEAEMDERFNSAAQLARGVAAMARIESLAATTTDKRIDPHAQLEALRQMVLAMVEDIRVVLLKLAERTHALRCAASPAP
EGDDPDERAALREALGQQARELFAPLANRLGVWQIKWEMEDWAFRYLEPETYRTIAAQLDEKRADREAYILGIIERLKAE
LALHGIEAEVTGRPKHIASIVNKMRRKHLSFEQLYDIRAVRVLVRQEIDCYTVLGLVHNLWQPIPGEFDDYISQPKSNDY
RSLHTAVIGPEDRALEVQIRTFDMHRHAELGVAAHWRYKESGKQDAQYEEKIAWLRRILEWKDDVADSSEFREQFKTELF
HDQVYVLTPQGRVVALDRGATPVDFAYAVHTDLGHRCRGAKVNGAMVPLNTVLDNSQRVEIVTAKEGTPSRDWLNPALGY
LATHRARSKVRAWFRQRDVGEQVNLGRSLLDKELKRLGVGDVNHEKLAQRLKFSSVDEFFAALGRGDVGQRDLVGALQEP
GKAPPALAPTAKPRSRPGSGSPVVIPGLGDVPLTMARCCKPRPPEAIVAYTTVGRGVTVHRADCASLRRANQARQMPAEW
ALDLGTGFEVDIRLKAFDRQGLLRDVSDVIAKDKLDVLRVNTETRGEYAQMQLTVRIKELQQLSRLLARLQHVQNVVEVR
RS

Sequences:

>Translated_722_residues
MNEIVRPRCYEATDRESARQALARVLPVAENDLPWRALDYAYERLGGSEAFAHSVGSALIAAELKVGADAVAAALLHACL
GDEAEMDERFNSAAQLARGVAAMARIESLAATTTDKRIDPHAQLEALRQMVLAMVEDIRVVLLKLAERTHALRCAASPAP
EGDDPDERAALREALGQQARELFAPLANRLGVWQIKWEMEDWAFRYLEPETYRTIAAQLDEKRADREAYILGIIERLKAE
LALHGIEAEVTGRPKHIASIVNKMRRKHLSFEQLYDIRAVRVLVRQEIDCYTVLGLVHNLWQPIPGEFDDYISQPKSNDY
RSLHTAVIGPEDRALEVQIRTFDMHRHAELGVAAHWRYKESGKQDAQYEEKIAWLRRILEWKDDVADSSEFREQFKTELF
HDQVYVLTPQGRVVALDRGATPVDFAYAVHTDLGHRCRGAKVNGAMVPLNTVLDNSQRVEIVTAKEGTPSRDWLNPALGY
LATHRARSKVRAWFRQRDVGEQVNLGRSLLDKELKRLGVGDVNHEKLAQRLKFSSVDEFFAALGRGDVGQRDLVGALQEP
GKAPPALAPTAKPRSRPGSGSPVVIPGLGDVPLTMARCCKPRPPEAIVAYTTVGRGVTVHRADCASLRRANQARQMPAEW
ALDLGTGFEVDIRLKAFDRQGLLRDVSDVIAKDKLDVLRVNTETRGEYAQMQLTVRIKELQQLSRLLARLQHVQNVVEVR
RS
>Mature_722_residues
MNEIVRPRCYEATDRESARQALARVLPVAENDLPWRALDYAYERLGGSEAFAHSVGSALIAAELKVGADAVAAALLHACL
GDEAEMDERFNSAAQLARGVAAMARIESLAATTTDKRIDPHAQLEALRQMVLAMVEDIRVVLLKLAERTHALRCAASPAP
EGDDPDERAALREALGQQARELFAPLANRLGVWQIKWEMEDWAFRYLEPETYRTIAAQLDEKRADREAYILGIIERLKAE
LALHGIEAEVTGRPKHIASIVNKMRRKHLSFEQLYDIRAVRVLVRQEIDCYTVLGLVHNLWQPIPGEFDDYISQPKSNDY
RSLHTAVIGPEDRALEVQIRTFDMHRHAELGVAAHWRYKESGKQDAQYEEKIAWLRRILEWKDDVADSSEFREQFKTELF
HDQVYVLTPQGRVVALDRGATPVDFAYAVHTDLGHRCRGAKVNGAMVPLNTVLDNSQRVEIVTAKEGTPSRDWLNPALGY
LATHRARSKVRAWFRQRDVGEQVNLGRSLLDKELKRLGVGDVNHEKLAQRLKFSSVDEFFAALGRGDVGQRDLVGALQEP
GKAPPALAPTAKPRSRPGSGSPVVIPGLGDVPLTMARCCKPRPPEAIVAYTTVGRGVTVHRADCASLRRANQARQMPAEW
ALDLGTGFEVDIRLKAFDRQGLLRDVSDVIAKDKLDVLRVNTETRGEYAQMQLTVRIKELQQLSRLLARLQHVQNVVEVR
RS

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which i

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Escherichia coli, GI1789147, Length=688, Percent_Identity=43.0232558139535, Blast_Score=556, Evalue=1e-159,
Organism=Escherichia coli, GI1790082, Length=695, Percent_Identity=35.5395683453237, Blast_Score=414, Evalue=1e-117,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 80947; Mature: 80947

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEIVRPRCYEATDRESARQALARVLPVAENDLPWRALDYAYERLGGSEAFAHSVGSALI
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
AAELKVGADAVAAALLHACLGDEAEMDERFNSAAQLARGVAAMARIESLAATTTDKRIDP
HHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
HAQLEALRQMVLAMVEDIRVVLLKLAERTHALRCAASPAPEGDDPDERAALREALGQQAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
ELFAPLANRLGVWQIKWEMEDWAFRYLEPETYRTIAAQLDEKRADREAYILGIIERLKAE
HHHHHHHHCCCEEEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LALHGIEAEVTGRPKHIASIVNKMRRKHLSFEQLYDIRAVRVLVRQEIDCYTVLGLVHNL
HHHHCCCEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
WQPIPGEFDDYISQPKSNDYRSLHTAVIGPEDRALEVQIRTFDMHRHAELGVAAHWRYKE
CCCCCCCHHHHHCCCCCCCHHHHHHHEECCCCCEEEEEEEEECCHHHHCCCHHEEECCHH
SGKQDAQYEEKIAWLRRILEWKDDVADSSEFREQFKTELFHDQVYVLTPQGRVVALDRGA
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEECCCCCEEEEECCC
TPVDFAYAVHTDLGHRCRGAKVNGAMVPLNTVLDNSQRVEIVTAKEGTPSRDWLNPALGY
CCCHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHH
LATHRARSKVRAWFRQRDVGEQVNLGRSLLDKELKRLGVGDVNHEKLAQRLKFSSVDEFF
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHH
AALGRGDVGQRDLVGALQEPGKAPPALAPTAKPRSRPGSGSPVVIPGLGDVPLTMARCCK
HHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHCC
PRPPEAIVAYTTVGRGVTVHRADCASLRRANQARQMPAEWALDLGTGFEVDIRLKAFDRQ
CCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEEEEECCCC
GLLRDVSDVIAKDKLDVLRVNTETRGEYAQMQLTVRIKELQQLSRLLARLQHVQNVVEVR
CHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RS
CC
>Mature Secondary Structure
MNEIVRPRCYEATDRESARQALARVLPVAENDLPWRALDYAYERLGGSEAFAHSVGSALI
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
AAELKVGADAVAAALLHACLGDEAEMDERFNSAAQLARGVAAMARIESLAATTTDKRIDP
HHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
HAQLEALRQMVLAMVEDIRVVLLKLAERTHALRCAASPAPEGDDPDERAALREALGQQAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
ELFAPLANRLGVWQIKWEMEDWAFRYLEPETYRTIAAQLDEKRADREAYILGIIERLKAE
HHHHHHHHCCCEEEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LALHGIEAEVTGRPKHIASIVNKMRRKHLSFEQLYDIRAVRVLVRQEIDCYTVLGLVHNL
HHHHCCCEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
WQPIPGEFDDYISQPKSNDYRSLHTAVIGPEDRALEVQIRTFDMHRHAELGVAAHWRYKE
CCCCCCCHHHHHCCCCCCCHHHHHHHEECCCCCEEEEEEEEECCHHHHCCCHHEEECCHH
SGKQDAQYEEKIAWLRRILEWKDDVADSSEFREQFKTELFHDQVYVLTPQGRVVALDRGA
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEECCCCCEEEEECCC
TPVDFAYAVHTDLGHRCRGAKVNGAMVPLNTVLDNSQRVEIVTAKEGTPSRDWLNPALGY
CCCHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHH
LATHRARSKVRAWFRQRDVGEQVNLGRSLLDKELKRLGVGDVNHEKLAQRLKFSSVDEFF
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHH
AALGRGDVGQRDLVGALQEPGKAPPALAPTAKPRSRPGSGSPVVIPGLGDVPLTMARCCK
HHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHCC
PRPPEAIVAYTTVGRGVTVHRADCASLRRANQARQMPAEWALDLGTGFEVDIRLKAFDRQ
CCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEEEEECCCC
GLLRDVSDVIAKDKLDVLRVNTETRGEYAQMQLTVRIKELQQLSRLLARLQHVQNVVEVR
CHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RS
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]