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The map label for this gene is selU [H]

Identifier: 74317775

GI number: 74317775

Start: 1847923

End: 1848963

Strand: Direct

Name: selU [H]

Synonym: Tbd_1757

Alternate gene names: 74317775

Gene position: 1847923-1848963 (Clockwise)

Preceding gene: 74317774

Following gene: 74317781

Centisome position: 63.51

GC content: 69.45

Gene sequence:

>1041_bases
TTGCCCGTCACCGTCGCAGAACTTCCCGCCTTCGCACACATCATCGATGCGCGTTCCCCCGGCGAGTTTGCCGAGGACCA
CATACCGGGCGCGATCAATCTTCCGGTGCTGGACGACGCCGAACGCGCGCGCGTCGGCACGCTCTACAAGCAGCGTTCGA
GCTTCGAGGCGAAGAAGCTCGGCGCCGCGCTGGTCTCGCGCAACATTGCCGCGCACCTCGACGCATGGTTCGCCGACAAG
CCGAAGTCCTATCGTCCGCTGGTCTACTGCTGGCGCGGCGGCAGCCGCAGCGGCGCGCTGACGCACGTGCTGCAGAAGAT
CGGCTTCGCCGCCGAGCAGCTCGACGGCGGCTACAAGGCCTATCGCCGCCATGTCGTGAGCGAGCTCGACCGCCTGCCCG
CGCTGTTTCGTTTTCGCGTGGTCTGCGGCCCGACCGGCAGCGGGAAGAGCCGGCTTCTGCATGCGCTTCGCGAAGCCGGC
GCGCAGGTGCTCGATCTCGAAGGCCTCGCCGCGCACCGCGGGTCGCTGCTCGGTGCGCTCCCGGGGCAACCCCAGCCCTC
GCAGAAGCGCTTCGAGAGCGCGATCTGGTCGGCGCTGACGCACTTCGATGCCGGCGCCACGGTCTTCGTCGAATCGGAAA
GCAAGAAGATCGGCGCCCTGCAGGTGCCGGATGCCCTCATTCGCGCGATGCGCGCGAGCCCCTGCGTACGGCTCGACGTG
CCGCTCGACGCCCGCGTGGGCCTGTTGAGCGAAGACTACGCGCATTTCCTCGTCGACCCGCAGACGCTCAACCGGCAGCT
CGGCCATCTCGCGGGACTGCGCGGCGGCGAGACCGTCGCCGCTTGGCAGGCCTTGGCCGCACGCGGCGCGTGGAACGAAC
TGGTGGCCGACCTGCTGGCGCAGCACTACGATCCGGCGTATTTCAAGTCGCTGTCGCACAACTACCCGCCGACCGCCGCC
GACCGGACGTTTCACGCCGCCGATCTCTCGCCTGCCGGCCTGCGCGACCTCGCCCGCGCGATCCTCGACGCCGCGCCCTG
A

Upstream 100 bases:

>100_bases
GCTACGCGGCGCCGAGCTTGCGCTGCGGCTCGCGCCACGCCGCAGCGCCGTCCGCATGGGACAATAGACGCTGTCCGGAT
TTCCGAAAGTCGAACTGCAC

Downstream 100 bases:

>100_bases
GCCGCGGGCGCCTGGCTGCAAAACCCTAGCTGCGCCTCACCTCGACGACGTTCTGCACGTGCTGCAGGCGGGCCAGCAGG
CGGGACAGCTGCTGCAGCTC

Product: tRNA 2-selenouridine synthase

Products: NA

Alternate protein names: Selenophosphate-dependent tRNA 2-selenouridine synthase [H]

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MPVTVAELPAFAHIIDARSPGEFAEDHIPGAINLPVLDDAERARVGTLYKQRSSFEAKKLGAALVSRNIAAHLDAWFADK
PKSYRPLVYCWRGGSRSGALTHVLQKIGFAAEQLDGGYKAYRRHVVSELDRLPALFRFRVVCGPTGSGKSRLLHALREAG
AQVLDLEGLAAHRGSLLGALPGQPQPSQKRFESAIWSALTHFDAGATVFVESESKKIGALQVPDALIRAMRASPCVRLDV
PLDARVGLLSEDYAHFLVDPQTLNRQLGHLAGLRGGETVAAWQALAARGAWNELVADLLAQHYDPAYFKSLSHNYPPTAA
DRTFHAADLSPAGLRDLARAILDAAP

Sequences:

>Translated_346_residues
MPVTVAELPAFAHIIDARSPGEFAEDHIPGAINLPVLDDAERARVGTLYKQRSSFEAKKLGAALVSRNIAAHLDAWFADK
PKSYRPLVYCWRGGSRSGALTHVLQKIGFAAEQLDGGYKAYRRHVVSELDRLPALFRFRVVCGPTGSGKSRLLHALREAG
AQVLDLEGLAAHRGSLLGALPGQPQPSQKRFESAIWSALTHFDAGATVFVESESKKIGALQVPDALIRAMRASPCVRLDV
PLDARVGLLSEDYAHFLVDPQTLNRQLGHLAGLRGGETVAAWQALAARGAWNELVADLLAQHYDPAYFKSLSHNYPPTAA
DRTFHAADLSPAGLRDLARAILDAAP
>Mature_345_residues
PVTVAELPAFAHIIDARSPGEFAEDHIPGAINLPVLDDAERARVGTLYKQRSSFEAKKLGAALVSRNIAAHLDAWFADKP
KSYRPLVYCWRGGSRSGALTHVLQKIGFAAEQLDGGYKAYRRHVVSELDRLPALFRFRVVCGPTGSGKSRLLHALREAGA
QVLDLEGLAAHRGSLLGALPGQPQPSQKRFESAIWSALTHFDAGATVFVESESKKIGALQVPDALIRAMRASPCVRLDVP
LDARVGLLSEDYAHFLVDPQTLNRQLGHLAGLRGGETVAAWQALAARGAWNELVADLLAQHYDPAYFKSLSHNYPPTAAD
RTFHAADLSPAGLRDLARAILDAAP

Specific function: Catalyzes the transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA [H]

COG id: COG2603

COG function: function code R; Predicted ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1786712, Length=247, Percent_Identity=36.4372469635627, Blast_Score=129, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR017582 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: NA

Molecular weight: Translated: 37424; Mature: 37293

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: PS00380 RHODANESE_1 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVTVAELPAFAHIIDARSPGEFAEDHIPGAINLPVLDDAERARVGTLYKQRSSFEAKKL
CCCCHHHHHHHHHHHCCCCCCCHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHCHHHHHH
GAALVSRNIAAHLDAWFADKPKSYRPLVYCWRGGSRSGALTHVLQKIGFAAEQLDGGYKA
HHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHH
YRRHVVSELDRLPALFRFRVVCGPTGSGKSRLLHALREAGAQVLDLEGLAAHRGSLLGAL
HHHHHHHHHHHCHHHHHEEEEECCCCCCHHHHHHHHHHCCCCEEECCHHHHHCCCEEECC
PGQPQPSQKRFESAIWSALTHFDAGATVFVESESKKIGALQVPDALIRAMRASPCVRLDV
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEECCHHHHHHHHCCCCEEEEC
PLDARVGLLSEDYAHFLVDPQTLNRQLGHLAGLRGGETVAAWQALAARGAWNELVADLLA
CCCCCCCCHHHHHHHHEECHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHH
QHYDPAYFKSLSHNYPPTAADRTFHAADLSPAGLRDLARAILDAAP
HHCCHHHHHHHCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
PVTVAELPAFAHIIDARSPGEFAEDHIPGAINLPVLDDAERARVGTLYKQRSSFEAKKL
CCCHHHHHHHHHHHCCCCCCCHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHCHHHHHH
GAALVSRNIAAHLDAWFADKPKSYRPLVYCWRGGSRSGALTHVLQKIGFAAEQLDGGYKA
HHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHH
YRRHVVSELDRLPALFRFRVVCGPTGSGKSRLLHALREAGAQVLDLEGLAAHRGSLLGAL
HHHHHHHHHHHCHHHHHEEEEECCCCCCHHHHHHHHHHCCCCEEECCHHHHHCCCEEECC
PGQPQPSQKRFESAIWSALTHFDAGATVFVESESKKIGALQVPDALIRAMRASPCVRLDV
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEECCHHHHHHHHCCCCEEEEC
PLDARVGLLSEDYAHFLVDPQTLNRQLGHLAGLRGGETVAAWQALAARGAWNELVADLLA
CCCCCCCCHHHHHHHHEECHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHH
QHYDPAYFKSLSHNYPPTAADRTFHAADLSPAGLRDLARAILDAAP
HHCCHHHHHHHCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]